l arginine r0k0  (Millipore)


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  • 90

    Structured Review

    Millipore l arginine r0k0
    L Arginine R0k0, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l arginine r0k0/product/Millipore
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    l arginine r0k0 - by Bioz Stars, 2020-04
    90/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Quantitative phosphoproteomic analysis of acquired cancer drug resistance to pazopanib and dasatinib
    Article Snippet: For SILAC experiments, A204 cells and resistant sublines were cultured in SILAC DMEM media (Thermo Fisher Scientific) supplemented with light lysine and arginine (R0K0) (Sigma) and heavy lysine and arginine (R10K8) (Goss Scientific), respectively. .. To generate resistant sublines, A204 cells were grown initially in DMEM media containing dasatinib and pazopanib (LC laboratories) at a concentration of 500 nM .

    Labeling:

    Article Title: Regulation of Platelet Derived Growth Factor Signaling by Leukocyte Common Antigen-related (LAR) Protein Tyrosine Phosphatase: A Quantitative Phosphoproteomics Study *
    Article Snippet: .. For SILAC labeling, cells were cultured in SILAC DMEM (Thermo Fisher Scientific, Loughborough, UK) supplemented with either “light” isotopically normal l -Lysine and l -Arginine (R0K0) (Sigma-Aldrich), “medium” 13 C6 l -Arginine and 4,4,5,5-D4 l -Lysine (R6K4), or “heavy” 13 C6 15 N4 l -Arginine and 13 C6 15 N2 l -Lysine (R10K8) (Goss Scientific, Crewe, UK), with 0.5 mg/ml proline (Sigma), 0.1 mg/ml streptomycin, 100 U/ml penicillin, 250 μg/ml amphotericin B and 10% v/v dialyzed fetal bovine serum (Labtech International, East Sussex, UK). .. Cells were grown in SILAC media for seven doubling times before being analyzed for incorporation efficiency of the SILAC amino acids (see ).

    Cell Culture:

    Article Title: Regulation of Platelet Derived Growth Factor Signaling by Leukocyte Common Antigen-related (LAR) Protein Tyrosine Phosphatase: A Quantitative Phosphoproteomics Study *
    Article Snippet: .. For SILAC labeling, cells were cultured in SILAC DMEM (Thermo Fisher Scientific, Loughborough, UK) supplemented with either “light” isotopically normal l -Lysine and l -Arginine (R0K0) (Sigma-Aldrich), “medium” 13 C6 l -Arginine and 4,4,5,5-D4 l -Lysine (R6K4), or “heavy” 13 C6 15 N4 l -Arginine and 13 C6 15 N2 l -Lysine (R10K8) (Goss Scientific, Crewe, UK), with 0.5 mg/ml proline (Sigma), 0.1 mg/ml streptomycin, 100 U/ml penicillin, 250 μg/ml amphotericin B and 10% v/v dialyzed fetal bovine serum (Labtech International, East Sussex, UK). .. Cells were grown in SILAC media for seven doubling times before being analyzed for incorporation efficiency of the SILAC amino acids (see ).

    Article Title: Quantitative phosphoproteomic analysis of acquired cancer drug resistance to pazopanib and dasatinib
    Article Snippet: .. For SILAC experiments, A204 cells and resistant sublines were cultured in SILAC DMEM media (Thermo Fisher Scientific) supplemented with light lysine and arginine (R0K0) (Sigma) and heavy lysine and arginine (R10K8) (Goss Scientific), respectively. .. To generate resistant sublines, A204 cells were grown initially in DMEM media containing dasatinib and pazopanib (LC laboratories) at a concentration of 500 nM .

    Article Title: Novel Binding Partners and Differentially Regulated Phosphorylation Sites Clarify Eps8 as a Multi-Functional Adaptor
    Article Snippet: .. For SILAC labelling, 293T cells were cultured in SILAC DMEM (Thermo Fisher Scientific) supplemented with either 0.1 mg/ml “light” isotopically normal L-Lysine and L-Arginine (R0K0) (Sigma), “medium” 13 C6 L-Lysine and 4,4,5,5-D4 L-Lysine (R6K4), or “heavy” 13 C6 15 N4 L-Arginine and 13 C6 15 N2 L-Lysine (R10K8) (Goss Scientific), 0.5 mg/ml proline (Sigma), 0.1 mg/ml streptomycin, 0.2 U/ml penicillin, and 10% v/v dialysed fetal bovine serum (Labtech International). .. Cloning and Transfection The human open reading frames for Eps8 and IRS4 were supplied in Gateway (Invitrogen™) pDONR vectors from Open Biosystems.

    Mouse Assay:

    Article Title: Regulation of Platelet Derived Growth Factor Signaling by Leukocyte Common Antigen-related (LAR) Protein Tyrosine Phosphatase: A Quantitative Phosphoproteomics Study *
    Article Snippet: Mouse embryonic fibroblasts (MEFs) from mice where the LAR phosphatase domains had been deleted (LARΔP) and from littermate wild-type (WT) , were used in the study (both cell types are kind gifts from Wiljan J.A.J. .. For SILAC labeling, cells were cultured in SILAC DMEM (Thermo Fisher Scientific, Loughborough, UK) supplemented with either “light” isotopically normal l -Lysine and l -Arginine (R0K0) (Sigma-Aldrich), “medium” 13 C6 l -Arginine and 4,4,5,5-D4 l -Lysine (R6K4), or “heavy” 13 C6 15 N4 l -Arginine and 13 C6 15 N2 l -Lysine (R10K8) (Goss Scientific, Crewe, UK), with 0.5 mg/ml proline (Sigma), 0.1 mg/ml streptomycin, 100 U/ml penicillin, 250 μg/ml amphotericin B and 10% v/v dialyzed fetal bovine serum (Labtech International, East Sussex, UK).