l arginine ethyl ester dihydrochloride  (Millipore)


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    Name:
    L Arginine ethyl ester dihydrochloride
    Description:

    Catalog Number:
    a2883
    Price:
    None
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    Structured Review

    Millipore l arginine ethyl ester dihydrochloride
    L Arginine ethyl ester dihydrochloride

    https://www.bioz.com/result/l arginine ethyl ester dihydrochloride/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    l arginine ethyl ester dihydrochloride - by Bioz Stars, 2020-04
    99/100 stars

    Images

    1) Product Images from "An engineered l-arginine sensor of Chlamydia pneumoniae enables arginine-adjustable transcription control in mammalian cells and mice"

    Article Title: An engineered l-arginine sensor of Chlamydia pneumoniae enables arginine-adjustable transcription control in mammalian cells and mice

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkm652

    Impact of l -arginine and its derivatives on ART-controlled gene expression. ( A ) The structure of the l -arginine derivatives used in this study. ( B ) Sixty hours after co-transfection with pSH91 (P SV40 -ARG2-pA) and pSH93 (P ART1 -SEAP-pA), SEAP expression of CHO-K1 was measured. Cells were incubated in cell culture media containing 10 mg/l l -arginine and supplemented with 57.4, 574 or 5740 μM of l -arginine, l -ornithine, l -citrulline, agmatine, l -homoarginine, l -arginine methyl ester, l -arginine ethyl ester or l -canavanine (5740 μM correspond to 1 g/l l -arginine).
    Figure Legend Snippet: Impact of l -arginine and its derivatives on ART-controlled gene expression. ( A ) The structure of the l -arginine derivatives used in this study. ( B ) Sixty hours after co-transfection with pSH91 (P SV40 -ARG2-pA) and pSH93 (P ART1 -SEAP-pA), SEAP expression of CHO-K1 was measured. Cells were incubated in cell culture media containing 10 mg/l l -arginine and supplemented with 57.4, 574 or 5740 μM of l -arginine, l -ornithine, l -citrulline, agmatine, l -homoarginine, l -arginine methyl ester, l -arginine ethyl ester or l -canavanine (5740 μM correspond to 1 g/l l -arginine).

    Techniques Used: Expressing, Cotransfection, Incubation, Cell Culture

    Related Articles

    Luminescence Assay:

    Article Title: SHMT2 drives glioma cell survival in the tumor microenvironment but imposes a dependence on glycine clearance
    Article Snippet: The following cell culture reagents were used: Neurobasal medium, N-2 and B-27 supplements from Invitrogen; Recombinant Human FGF basic (4114-TC) and EGF (236-EG) from R & D systems; DMEM and RPMI-1640 media, doxycycline (D9891) from Sigma; Leucine ethyl ester hydrochloride (61850), Arginine ethyl ester hydrochloride (A2883), Alanine ethyl ester hydrochloride (855669), Valine ethyl ester hydrochloride (220698), Lysine ethyl ester dihydrochloride (62880), Ethylamine (395064) from Sigma; Glycine ethyl ester hydrochloride (sc-295020) and Polybrene (sc-134220) from Santa Cruz. .. Additional materials used: formalin from VWR; Borg Decloaker RTU solution and pressurized Decloaking Chamber from Biocare Medical; Prolong Gold Antifade reagent from Invitrogen; CellTiter-Glo Luminescent Assay from Promega; U14 C-Serine from MP Biomedicals; Matrigel (356230) from BD Biosciences.

    Recombinant:

    Article Title: SHMT2 drives glioma cell survival in the tumor microenvironment but imposes a dependence on glycine clearance
    Article Snippet: .. The following cell culture reagents were used: Neurobasal medium, N-2 and B-27 supplements from Invitrogen; Recombinant Human FGF basic (4114-TC) and EGF (236-EG) from R & D systems; DMEM and RPMI-1640 media, doxycycline (D9891) from Sigma; Leucine ethyl ester hydrochloride (61850), Arginine ethyl ester hydrochloride (A2883), Alanine ethyl ester hydrochloride (855669), Valine ethyl ester hydrochloride (220698), Lysine ethyl ester dihydrochloride (62880), Ethylamine (395064) from Sigma; Glycine ethyl ester hydrochloride (sc-295020) and Polybrene (sc-134220) from Santa Cruz. .. Additional materials used: formalin from VWR; Borg Decloaker RTU solution and pressurized Decloaking Chamber from Biocare Medical; Prolong Gold Antifade reagent from Invitrogen; CellTiter-Glo Luminescent Assay from Promega; U14 C-Serine from MP Biomedicals; Matrigel (356230) from BD Biosciences.

    other:

    Article Title: An engineered l-arginine sensor of Chlamydia pneumoniae enables arginine-adjustable transcription control in mammalian cells and mice
    Article Snippet: Regulating l -arginine and l -arginine analogs l -arginine stock solution was prepared in water by adjusting 50 g/l l -arginine base (0.287 M, AppliChem, Darmstadt, Germany, Cat. No. A3653,0100) to pH 7.2 with HCl and used at the final concentrations indicated. l -ornithine hydrochloride (Fluka, Buchs, Switzerland, Cat. No. 75470) and l -citrulline (Fluka, Buchs, Switzerland, Cat. No. 27510), l -homoarginine hydrochloride (Acros Organics, Basel, Switzerland, Cat. No. 169090050) were dissolved in water (0.287 M) with pH adjusted to 7.2 by NaOH. l -arginine methyl ester dihydrochloride (Fluka, Buchs, Switzerland, Cat. No. 11030), l -arginine ethyl ester dihydrochloride (Sigma-Aldrich, Steinheim, Germany, Cat. No. A2883), agmatine sulfate (Sigma-Aldrich, Cat. No. A7127) and l -canavanine sulfate (Sigma-Aldrich, Cat. No. C9758) stock solutions were prepared by adjusting the pH of a 0.144 M solution to pH 7.2.

    Cell Culture:

    Article Title: SHMT2 drives glioma cell survival in the tumor microenvironment but imposes a dependence on glycine clearance
    Article Snippet: .. The following cell culture reagents were used: Neurobasal medium, N-2 and B-27 supplements from Invitrogen; Recombinant Human FGF basic (4114-TC) and EGF (236-EG) from R & D systems; DMEM and RPMI-1640 media, doxycycline (D9891) from Sigma; Leucine ethyl ester hydrochloride (61850), Arginine ethyl ester hydrochloride (A2883), Alanine ethyl ester hydrochloride (855669), Valine ethyl ester hydrochloride (220698), Lysine ethyl ester dihydrochloride (62880), Ethylamine (395064) from Sigma; Glycine ethyl ester hydrochloride (sc-295020) and Polybrene (sc-134220) from Santa Cruz. .. Additional materials used: formalin from VWR; Borg Decloaker RTU solution and pressurized Decloaking Chamber from Biocare Medical; Prolong Gold Antifade reagent from Invitrogen; CellTiter-Glo Luminescent Assay from Promega; U14 C-Serine from MP Biomedicals; Matrigel (356230) from BD Biosciences.

    Purification:

    Article Title: A change in the aggregation pathway of bovine serum albumin in the presence of arginine and its derivatives
    Article Snippet: .. Materials BSA (catalogue no. A7638, 99+% of purity), L-arginine monohydrochloride (Arg), L-arginine amide hydrochloride (ArgEE) and L-arginine ethyl ester hydrochloride (ArgEE) were purchased from Sigma–Aldrich and used without further purification. .. All solutions for the experiments were prepared using deionized water obtained with Easy-Pure II RF system (Barnstead, USA).

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