l arginine 13c6 15n4 hydrochloride  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    L Arginine 13C6 15N4 hydrochloride
    Description:

    Catalog Number:
    608033
    Price:
    None
    Buy from Supplier


    Structured Review

    Millipore l arginine 13c6 15n4 hydrochloride
    L Arginine 13C6 15N4 hydrochloride

    https://www.bioz.com/result/l arginine 13c6 15n4 hydrochloride/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    l arginine 13c6 15n4 hydrochloride - by Bioz Stars, 2020-03
    99/100 stars

    Images

    Related Articles

    Centrifugation:

    Article Title: Selective usage of isozymes for stress response
    Article Snippet: Saccharomyces cerevisiae BY4741 strain (HL813Y) was cultured in synthetic complete media (20 mL) with heavy lysine (Sigma 608041) (0.086 g L−1 ) and arginine (Sigma 608033) (0.086 g L−1 ) till OD600 reached approximately 0.5. .. Cells were harvested by centrifugation at 5000 rpm for 5 min and lysed with 600 μL of glass beads (OPS Diagnostics BAWG400-200-04) and 1mL lysis buffer containing 50 mM Tris pH 8.0, 150 mM sodium chloride and 1 mM phenylmethanesulfonyl fluoride.

    Sample Prep:

    Article Title: Selective usage of isozymes for stress response
    Article Snippet: Paragraph title: Sample preparation for SILAC study. ... Saccharomyces cerevisiae BY4741 strain (HL813Y) was cultured in synthetic complete media (20 mL) with heavy lysine (Sigma 608041) (0.086 g L−1 ) and arginine (Sigma 608033) (0.086 g L−1 ) till OD600 reached approximately 0.5.

    Labeling:

    Article Title: PRMT1-mediated methylation of the microprocessor-associated proteins regulates microRNA biogenesis
    Article Snippet: .. For standard SILAC labeling, HeLa cells were grown in ‘‘Light’’ and ‘‘Heavy’’ SILAC DMEM (Thermo Fisher Scientific, 88420) supplemented with either L-arginine and L-lysine, or their heavy isotope-counterparts l -arginine-13 C6 , 15 N4 hydrochloride (Arg10, Sigma, 608033) and L-lysine-13 C6 , 15 N2 hydrochloride (Lys 8, Sigma, 608041) ( ). .. All media were supplemented with 10% dialyzed FBS (26400-044 Gibco, Life Technology), 1% glutamine, 100 U/ml Penicillin and 100 mg/ml Streptomycin.

    Article Title: Cell-surface HSP70 associates with thrombomodulin in endothelial cells
    Article Snippet: .. Briefly, cells were grown in DMEM/F12 SILAC (stable isotope labeling by amino acids in cell culture) Media (Thermo Scientific, A2494301) supplemented with EGM, 2% dialyzed FBS, arginine 13 C6 , 15N4 (Sigma #608033), lysine 13 C6 15 N2 (Sigma#608041) for heavy media, or regular arginine and lysine for light media. .. Cells were replicated until passage five, when > 90% of proteins were labeled with heavy amino acids.

    Generated:

    Article Title: PRMT1-mediated methylation of the microprocessor-associated proteins regulates microRNA biogenesis
    Article Snippet: HeLa Flp-In T-REx cells were in-house generated according to the guidelines of Thermo Fisher Scientific and grown in DMEM supplemented with 10% FBS Tetracycline free (Euroclone, ECS01822), 1% glutamine and 100 U/ml Penicillin and Streptomycin. .. For standard SILAC labeling, HeLa cells were grown in ‘‘Light’’ and ‘‘Heavy’’ SILAC DMEM (Thermo Fisher Scientific, 88420) supplemented with either L-arginine and L-lysine, or their heavy isotope-counterparts l -arginine-13 C6 , 15 N4 hydrochloride (Arg10, Sigma, 608033) and L-lysine-13 C6 , 15 N2 hydrochloride (Lys 8, Sigma, 608041) ( ).

    Cell Culture:

    Article Title: PRMT1-mediated methylation of the microprocessor-associated proteins regulates microRNA biogenesis
    Article Snippet: Paragraph title: Cell culture, heavy methyl SILAC and SILAC labeling of cells ... For standard SILAC labeling, HeLa cells were grown in ‘‘Light’’ and ‘‘Heavy’’ SILAC DMEM (Thermo Fisher Scientific, 88420) supplemented with either L-arginine and L-lysine, or their heavy isotope-counterparts l -arginine-13 C6 , 15 N4 hydrochloride (Arg10, Sigma, 608033) and L-lysine-13 C6 , 15 N2 hydrochloride (Lys 8, Sigma, 608041) ( ).

    Article Title: Selective usage of isozymes for stress response
    Article Snippet: .. Saccharomyces cerevisiae BY4741 strain (HL813Y) was cultured in synthetic complete media (20 mL) with heavy lysine (Sigma 608041) (0.086 g L−1 ) and arginine (Sigma 608033) (0.086 g L−1 ) till OD600 reached approximately 0.5. .. The BY4741 strain treated with 10 nM Rapamycin was cultured in synthetic complete media with light lysine and arginine till OD600 reached approximately 0.5.

    Article Title: Cell-surface HSP70 associates with thrombomodulin in endothelial cells
    Article Snippet: .. Briefly, cells were grown in DMEM/F12 SILAC (stable isotope labeling by amino acids in cell culture) Media (Thermo Scientific, A2494301) supplemented with EGM, 2% dialyzed FBS, arginine 13 C6 , 15N4 (Sigma #608033), lysine 13 C6 15 N2 (Sigma#608041) for heavy media, or regular arginine and lysine for light media. .. Cells were replicated until passage five, when > 90% of proteins were labeled with heavy amino acids.

    Lysis:

    Article Title: Selective usage of isozymes for stress response
    Article Snippet: Saccharomyces cerevisiae BY4741 strain (HL813Y) was cultured in synthetic complete media (20 mL) with heavy lysine (Sigma 608041) (0.086 g L−1 ) and arginine (Sigma 608033) (0.086 g L−1 ) till OD600 reached approximately 0.5. .. Cells were harvested by centrifugation at 5000 rpm for 5 min and lysed with 600 μL of glass beads (OPS Diagnostics BAWG400-200-04) and 1mL lysis buffer containing 50 mM Tris pH 8.0, 150 mM sodium chloride and 1 mM phenylmethanesulfonyl fluoride.