kw 4209 cells  (Corning Life Sciences)

 
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    Corning Life Sciences kw 4209 cells
    ( a ) Schematic illustration of CM production. The total thawed cells recovered from a cryopreservation tube (approximately 1 × 10 6 cells) were directly seeded on a 150 mm-diameter dish at a density as shown in (#PT-5006: 7318 ± 99 cells/cm 2 , <t>KW-4209:</t> 6310 ± 310 cells/cm 2 ) and cultured for 96 h with the FBS-containing MEMα medium. Then, the medium was changed to produce CM. The photos show phase contrast images of cultured KW-4209 cells at the points indicated by the arrows 2 h after seeding and the time of collection of FBS+ CM. The bars indicate 100 μm (original magnification; 200×). The glucose concentration in the medium is shown as the mean ± range of the two lots, respectively. The gray dashed line indicates the initial glucose concentration of the MEMα medium. ( b ) Schematic illustration of CM production. Thawed cells were cultured with FBS-containing MEMα for 2 h to ensure the adhesion, the medium was changed to test CMs, and [ 3 H]-thymidine was added.
    Kw 4209 Cells, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kw 4209 cells/product/Corning Life Sciences
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    kw 4209 cells - by Bioz Stars, 2024-09
    86/100 stars

    Images

    1) Product Images from "Proposal of Simplified Standardization of the Cell-Growth-Promoting Activity of Human Adipose Tissue Mesenchymal Stromal Cell Culture Supernatants"

    Article Title: Proposal of Simplified Standardization of the Cell-Growth-Promoting Activity of Human Adipose Tissue Mesenchymal Stromal Cell Culture Supernatants

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms25105197

    ( a ) Schematic illustration of CM production. The total thawed cells recovered from a cryopreservation tube (approximately 1 × 10 6 cells) were directly seeded on a 150 mm-diameter dish at a density as shown in (#PT-5006: 7318 ± 99 cells/cm 2 , KW-4209: 6310 ± 310 cells/cm 2 ) and cultured for 96 h with the FBS-containing MEMα medium. Then, the medium was changed to produce CM. The photos show phase contrast images of cultured KW-4209 cells at the points indicated by the arrows 2 h after seeding and the time of collection of FBS+ CM. The bars indicate 100 μm (original magnification; 200×). The glucose concentration in the medium is shown as the mean ± range of the two lots, respectively. The gray dashed line indicates the initial glucose concentration of the MEMα medium. ( b ) Schematic illustration of CM production. Thawed cells were cultured with FBS-containing MEMα for 2 h to ensure the adhesion, the medium was changed to test CMs, and [ 3 H]-thymidine was added.
    Figure Legend Snippet: ( a ) Schematic illustration of CM production. The total thawed cells recovered from a cryopreservation tube (approximately 1 × 10 6 cells) were directly seeded on a 150 mm-diameter dish at a density as shown in (#PT-5006: 7318 ± 99 cells/cm 2 , KW-4209: 6310 ± 310 cells/cm 2 ) and cultured for 96 h with the FBS-containing MEMα medium. Then, the medium was changed to produce CM. The photos show phase contrast images of cultured KW-4209 cells at the points indicated by the arrows 2 h after seeding and the time of collection of FBS+ CM. The bars indicate 100 μm (original magnification; 200×). The glucose concentration in the medium is shown as the mean ± range of the two lots, respectively. The gray dashed line indicates the initial glucose concentration of the MEMα medium. ( b ) Schematic illustration of CM production. Thawed cells were cultured with FBS-containing MEMα for 2 h to ensure the adhesion, the medium was changed to test CMs, and [ 3 H]-thymidine was added.

    Techniques Used: Cell Culture, Concentration Assay

    Cell proliferation-promoting activities of CM after 1 year of refrigerated storage. Due to the unavailability of cells, the experiment was performed only with KW-4209. Gray dashed line indicates the activity of non-CM control medium. Asterisks indicate statistical significance; p < 0.05 by ANOVA and Fisher’s protected least significant difference (PLSD) test.
    Figure Legend Snippet: Cell proliferation-promoting activities of CM after 1 year of refrigerated storage. Due to the unavailability of cells, the experiment was performed only with KW-4209. Gray dashed line indicates the activity of non-CM control medium. Asterisks indicate statistical significance; p < 0.05 by ANOVA and Fisher’s protected least significant difference (PLSD) test.

    Techniques Used: Activity Assay

    Cell-proliferation-promoting activities of CM with young and aged cells ( n = 4 and 8, respectively). Due to the unavailability of cells, the experiment was performed only with KW-4209. Gray dashed line indicates the activity of non-CM control medium. Asterisks indicate statistical significance; p < 0.05 according to ANOVA and Fisher’s protected least significant difference (PLSD) test.
    Figure Legend Snippet: Cell-proliferation-promoting activities of CM with young and aged cells ( n = 4 and 8, respectively). Due to the unavailability of cells, the experiment was performed only with KW-4209. Gray dashed line indicates the activity of non-CM control medium. Asterisks indicate statistical significance; p < 0.05 according to ANOVA and Fisher’s protected least significant difference (PLSD) test.

    Techniques Used: Activity Assay

    Cell-proliferation-promoting activities of CM fractionated by molecular weight. FBS-containing CMs from #PT-5006 and KW-4209 were mixed and fractionated with limiting membranes. The activities were assayed with KW-4209 cells. In the preliminary experiment, the activities were not recovered with FBS-free CM. Asterisks indicates statistical significance; p < 0.05 according to ANOVA and Fisher’s protected least significant difference (PLSD) test.
    Figure Legend Snippet: Cell-proliferation-promoting activities of CM fractionated by molecular weight. FBS-containing CMs from #PT-5006 and KW-4209 were mixed and fractionated with limiting membranes. The activities were assayed with KW-4209 cells. In the preliminary experiment, the activities were not recovered with FBS-free CM. Asterisks indicates statistical significance; p < 0.05 according to ANOVA and Fisher’s protected least significant difference (PLSD) test.

    Techniques Used: Molecular Weight

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    • kw 4209 cells  (Corning Life Sciences)
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    Corning Life Sciences kw 4209 cells
    ( a ) Schematic illustration of CM production. The total thawed cells recovered from a cryopreservation tube (approximately 1 × 10 6 cells) were directly seeded on a 150 mm-diameter dish at a density as shown in (#PT-5006: 7318 ± 99 cells/cm 2 , <t>KW-4209:</t> 6310 ± 310 cells/cm 2 ) and cultured for 96 h with the FBS-containing MEMα medium. Then, the medium was changed to produce CM. The photos show phase contrast images of cultured KW-4209 cells at the points indicated by the arrows 2 h after seeding and the time of collection of FBS+ CM. The bars indicate 100 μm (original magnification; 200×). The glucose concentration in the medium is shown as the mean ± range of the two lots, respectively. The gray dashed line indicates the initial glucose concentration of the MEMα medium. ( b ) Schematic illustration of CM production. Thawed cells were cultured with FBS-containing MEMα for 2 h to ensure the adhesion, the medium was changed to test CMs, and [ 3 H]-thymidine was added.
    Kw 4209 Cells, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kw 4209 cells/product/Corning Life Sciences
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    kw 4209 cells - by Bioz Stars, 2024-09
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    ( a ) Schematic illustration of CM production. The total thawed cells recovered from a cryopreservation tube (approximately 1 × 10 6 cells) were directly seeded on a 150 mm-diameter dish at a density as shown in (#PT-5006: 7318 ± 99 cells/cm 2 , KW-4209: 6310 ± 310 cells/cm 2 ) and cultured for 96 h with the FBS-containing MEMα medium. Then, the medium was changed to produce CM. The photos show phase contrast images of cultured KW-4209 cells at the points indicated by the arrows 2 h after seeding and the time of collection of FBS+ CM. The bars indicate 100 μm (original magnification; 200×). The glucose concentration in the medium is shown as the mean ± range of the two lots, respectively. The gray dashed line indicates the initial glucose concentration of the MEMα medium. ( b ) Schematic illustration of CM production. Thawed cells were cultured with FBS-containing MEMα for 2 h to ensure the adhesion, the medium was changed to test CMs, and [ 3 H]-thymidine was added.

    Journal: International Journal of Molecular Sciences

    Article Title: Proposal of Simplified Standardization of the Cell-Growth-Promoting Activity of Human Adipose Tissue Mesenchymal Stromal Cell Culture Supernatants

    doi: 10.3390/ijms25105197

    Figure Lengend Snippet: ( a ) Schematic illustration of CM production. The total thawed cells recovered from a cryopreservation tube (approximately 1 × 10 6 cells) were directly seeded on a 150 mm-diameter dish at a density as shown in (#PT-5006: 7318 ± 99 cells/cm 2 , KW-4209: 6310 ± 310 cells/cm 2 ) and cultured for 96 h with the FBS-containing MEMα medium. Then, the medium was changed to produce CM. The photos show phase contrast images of cultured KW-4209 cells at the points indicated by the arrows 2 h after seeding and the time of collection of FBS+ CM. The bars indicate 100 μm (original magnification; 200×). The glucose concentration in the medium is shown as the mean ± range of the two lots, respectively. The gray dashed line indicates the initial glucose concentration of the MEMα medium. ( b ) Schematic illustration of CM production. Thawed cells were cultured with FBS-containing MEMα for 2 h to ensure the adhesion, the medium was changed to test CMs, and [ 3 H]-thymidine was added.

    Article Snippet: To estimate the growth-promoting activity of CM, #PT-5006 or KW-4209 cells were thawed and cultured with 10% FBS-containing MEMα for 2 h at 1.5 × 10 4 live cells/0.1 mL/well, 4.7 × 10 4 live cells/cm 2 (4 wells each; 3599, Corning 96-well cell culture plate; practical cultivation area, 0.32 cm 2 ) at 37 °C under 5% CO 2 .

    Techniques: Cell Culture, Concentration Assay

    Cell proliferation-promoting activities of CM after 1 year of refrigerated storage. Due to the unavailability of cells, the experiment was performed only with KW-4209. Gray dashed line indicates the activity of non-CM control medium. Asterisks indicate statistical significance; p < 0.05 by ANOVA and Fisher’s protected least significant difference (PLSD) test.

    Journal: International Journal of Molecular Sciences

    Article Title: Proposal of Simplified Standardization of the Cell-Growth-Promoting Activity of Human Adipose Tissue Mesenchymal Stromal Cell Culture Supernatants

    doi: 10.3390/ijms25105197

    Figure Lengend Snippet: Cell proliferation-promoting activities of CM after 1 year of refrigerated storage. Due to the unavailability of cells, the experiment was performed only with KW-4209. Gray dashed line indicates the activity of non-CM control medium. Asterisks indicate statistical significance; p < 0.05 by ANOVA and Fisher’s protected least significant difference (PLSD) test.

    Article Snippet: To estimate the growth-promoting activity of CM, #PT-5006 or KW-4209 cells were thawed and cultured with 10% FBS-containing MEMα for 2 h at 1.5 × 10 4 live cells/0.1 mL/well, 4.7 × 10 4 live cells/cm 2 (4 wells each; 3599, Corning 96-well cell culture plate; practical cultivation area, 0.32 cm 2 ) at 37 °C under 5% CO 2 .

    Techniques: Activity Assay

    Cell-proliferation-promoting activities of CM with young and aged cells ( n = 4 and 8, respectively). Due to the unavailability of cells, the experiment was performed only with KW-4209. Gray dashed line indicates the activity of non-CM control medium. Asterisks indicate statistical significance; p < 0.05 according to ANOVA and Fisher’s protected least significant difference (PLSD) test.

    Journal: International Journal of Molecular Sciences

    Article Title: Proposal of Simplified Standardization of the Cell-Growth-Promoting Activity of Human Adipose Tissue Mesenchymal Stromal Cell Culture Supernatants

    doi: 10.3390/ijms25105197

    Figure Lengend Snippet: Cell-proliferation-promoting activities of CM with young and aged cells ( n = 4 and 8, respectively). Due to the unavailability of cells, the experiment was performed only with KW-4209. Gray dashed line indicates the activity of non-CM control medium. Asterisks indicate statistical significance; p < 0.05 according to ANOVA and Fisher’s protected least significant difference (PLSD) test.

    Article Snippet: To estimate the growth-promoting activity of CM, #PT-5006 or KW-4209 cells were thawed and cultured with 10% FBS-containing MEMα for 2 h at 1.5 × 10 4 live cells/0.1 mL/well, 4.7 × 10 4 live cells/cm 2 (4 wells each; 3599, Corning 96-well cell culture plate; practical cultivation area, 0.32 cm 2 ) at 37 °C under 5% CO 2 .

    Techniques: Activity Assay

    Cell-proliferation-promoting activities of CM fractionated by molecular weight. FBS-containing CMs from #PT-5006 and KW-4209 were mixed and fractionated with limiting membranes. The activities were assayed with KW-4209 cells. In the preliminary experiment, the activities were not recovered with FBS-free CM. Asterisks indicates statistical significance; p < 0.05 according to ANOVA and Fisher’s protected least significant difference (PLSD) test.

    Journal: International Journal of Molecular Sciences

    Article Title: Proposal of Simplified Standardization of the Cell-Growth-Promoting Activity of Human Adipose Tissue Mesenchymal Stromal Cell Culture Supernatants

    doi: 10.3390/ijms25105197

    Figure Lengend Snippet: Cell-proliferation-promoting activities of CM fractionated by molecular weight. FBS-containing CMs from #PT-5006 and KW-4209 were mixed and fractionated with limiting membranes. The activities were assayed with KW-4209 cells. In the preliminary experiment, the activities were not recovered with FBS-free CM. Asterisks indicates statistical significance; p < 0.05 according to ANOVA and Fisher’s protected least significant difference (PLSD) test.

    Article Snippet: To estimate the growth-promoting activity of CM, #PT-5006 or KW-4209 cells were thawed and cultured with 10% FBS-containing MEMα for 2 h at 1.5 × 10 4 live cells/0.1 mL/well, 4.7 × 10 4 live cells/cm 2 (4 wells each; 3599, Corning 96-well cell culture plate; practical cultivation area, 0.32 cm 2 ) at 37 °C under 5% CO 2 .

    Techniques: Molecular Weight