kpni xhoi opened pbluescript ii sk  (Thermo Fisher)


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    Structured Review

    Thermo Fisher kpni xhoi opened pbluescript ii sk
    Kpni Xhoi Opened Pbluescript Ii Sk, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kpni xhoi opened pbluescript ii sk/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    kpni xhoi opened pbluescript ii sk - by Bioz Stars, 2020-04
    86/100 stars

    Related Products / Commonly Used Together

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    Related Articles

    Isolation:

    Article Title: Fungal Gene Expression on Demand: an Inducible, Tunable, and Metabolism-Independent Expression System for Aspergillus niger ▿ ▿ †
    Article Snippet: The respective 5′ PCR products were digested with KpnI and XhoI and ligated into KpnI-XhoI-opened pBluescript II SK(+) (Fermentas). .. A 1.7-kb XhoI-HindIII fragment of the A. oryzae pyrG gene was isolated from pMA172 , and the cfrX 3′ flanking sequences were digested with HindIII and NotI.

    Construct:

    Article Title: Fungal Gene Expression on Demand: an Inducible, Tunable, and Metabolism-Independent Expression System for Aspergillus niger ▿ ▿ †
    Article Snippet: Constructs to delete the cfrA (An08g08280), cfrB (An07g07170), cfrC (An16g05020), cfrE (An11g05330), cfrF (An18g03740), or cfrG (An17g02350) gene were made as follows: cfrX 5′ flanking sequences (length, ∼700 bp) were obtained as KpnI-XhoI fragments by PCR using genomic DNA from strain N402 as a template and the primer pairs cfrXP1KpnI and cfrXP2XhoI. cfrX 3′ flanking sequences (length, ∼700 bp) were obtained as HindIII-NotI fragments from N402 genomic DNA by PCR using the primer pairs cfrXP3HindIII and cfrXP4NotI (the only exception was cfrG ; there, the 5′ flanking sequence was NotI-HindIII and the 3′ flanking sequence was XhoI-KpnI). .. The respective 5′ PCR products were digested with KpnI and XhoI and ligated into KpnI-XhoI-opened pBluescript II SK(+) (Fermentas).

    Polymerase Chain Reaction:

    Article Title: Fungal Gene Expression on Demand: an Inducible, Tunable, and Metabolism-Independent Expression System for Aspergillus niger ▿ ▿ †
    Article Snippet: .. The respective 5′ PCR products were digested with KpnI and XhoI and ligated into KpnI-XhoI-opened pBluescript II SK(+) (Fermentas). .. A 1.7-kb XhoI-HindIII fragment of the A. oryzae pyrG gene was isolated from pMA172 , and the cfrX 3′ flanking sequences were digested with HindIII and NotI.

    Sequencing:

    Article Title: Fungal Gene Expression on Demand: an Inducible, Tunable, and Metabolism-Independent Expression System for Aspergillus niger ▿ ▿ †
    Article Snippet: Constructs to delete the cfrA (An08g08280), cfrB (An07g07170), cfrC (An16g05020), cfrE (An11g05330), cfrF (An18g03740), or cfrG (An17g02350) gene were made as follows: cfrX 5′ flanking sequences (length, ∼700 bp) were obtained as KpnI-XhoI fragments by PCR using genomic DNA from strain N402 as a template and the primer pairs cfrXP1KpnI and cfrXP2XhoI. cfrX 3′ flanking sequences (length, ∼700 bp) were obtained as HindIII-NotI fragments from N402 genomic DNA by PCR using the primer pairs cfrXP3HindIII and cfrXP4NotI (the only exception was cfrG ; there, the 5′ flanking sequence was NotI-HindIII and the 3′ flanking sequence was XhoI-KpnI). .. The respective 5′ PCR products were digested with KpnI and XhoI and ligated into KpnI-XhoI-opened pBluescript II SK(+) (Fermentas).

    Transformation Assay:

    Article Title: Fungal Gene Expression on Demand: an Inducible, Tunable, and Metabolism-Independent Expression System for Aspergillus niger ▿ ▿ †
    Article Snippet: The respective 5′ PCR products were digested with KpnI and XhoI and ligated into KpnI-XhoI-opened pBluescript II SK(+) (Fermentas). .. The pyrG − strain MA70.15 was used as a recipient strain for transformation.

    Over Expression:

    Article Title: Fungal Gene Expression on Demand: an Inducible, Tunable, and Metabolism-Independent Expression System for Aspergillus niger ▿ ▿ †
    Article Snippet: Paragraph title: Construction of cfr deletion and overexpression strains. ... The respective 5′ PCR products were digested with KpnI and XhoI and ligated into KpnI-XhoI-opened pBluescript II SK(+) (Fermentas).

    Plasmid Preparation:

    Article Title: Fungal Gene Expression on Demand: an Inducible, Tunable, and Metabolism-Independent Expression System for Aspergillus niger ▿ ▿ †
    Article Snippet: The respective 5′ PCR products were digested with KpnI and XhoI and ligated into KpnI-XhoI-opened pBluescript II SK(+) (Fermentas). .. Both fragments were ligated into XhoI-NotI-opened pBluescript II SK(+) vector containing the respective cfrX 5′ flanking sequence.