kpn 2i  (Thermo Fisher)


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    Name:
    Kpn2I BspEI 10 U µL
    Description:
    5 T ↓C C G G A 3 3 A G G C C ↑T 5 Thermo Scientific Kpn2I BspEI restriction enzyme recognizes T CCGGA sites and cuts best at 55°C in Tango buffer See Reaction Conditions for Restriction Enzymes for a table of enzyme activity conditions for double digestion and heat inactivation for this and other restriction enzymes Note Also available as a FastDigest enzyme for rapid DNA digestion Isoschizomers AccIII Aor13HI BseAI Bsp13I BspEI MroI Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes optimized to work in one of the buffers of the Five Buffer System In addition the universal Tango buffer is provided for convenience in double digestions All of the enzymes exhibit 100 activity in the recommended buffer and reaction conditions To ensure consistent performance Thermo Scientific restriction enzyme reaction buffers contain premixed BSA which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations Features• Superior quality stringent quality control and industry leading manufacturing process• Convenient color coded Five Buffer System• Includes universal Tango buffer for double digestions• BSA premixed in reaction buffers• Wide selection of restriction endonuclease specificitiesApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern blotting• Restriction fragment length polymorphism RFLP • SNPNote For methylation sensitivity refer to product specifications
    Catalog Number:
    er0531
    Price:
    None
    Applications:
    Cloning|Restriction Enzyme Cloning
    Category:
    Proteins Enzymes Peptides
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    Structured Review

    Thermo Fisher kpn 2i
    5 T ↓C C G G A 3 3 A G G C C ↑T 5 Thermo Scientific Kpn2I BspEI restriction enzyme recognizes T CCGGA sites and cuts best at 55°C in Tango buffer See Reaction Conditions for Restriction Enzymes for a table of enzyme activity conditions for double digestion and heat inactivation for this and other restriction enzymes Note Also available as a FastDigest enzyme for rapid DNA digestion Isoschizomers AccIII Aor13HI BseAI Bsp13I BspEI MroI Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes optimized to work in one of the buffers of the Five Buffer System In addition the universal Tango buffer is provided for convenience in double digestions All of the enzymes exhibit 100 activity in the recommended buffer and reaction conditions To ensure consistent performance Thermo Scientific restriction enzyme reaction buffers contain premixed BSA which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations Features• Superior quality stringent quality control and industry leading manufacturing process• Convenient color coded Five Buffer System• Includes universal Tango buffer for double digestions• BSA premixed in reaction buffers• Wide selection of restriction endonuclease specificitiesApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern blotting• Restriction fragment length polymorphism RFLP • SNPNote For methylation sensitivity refer to product specifications
    https://www.bioz.com/result/kpn 2i/product/Thermo Fisher
    Average 93 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    kpn 2i - by Bioz Stars, 2020-07
    93/100 stars

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    Related Articles

    Amplification:

    Article Title: Specific Antibody Fragment Ligand Traps Blocking FGF1 Activity
    Article Snippet: .. In the next step, amplified scFv DNA and pLEV113-Fc expression vector (LakePharma, Belmont, CA, USA), encoding the Fc domain of human IgG1 [ ], were digested using HindIII and Kpn2I restriction enzymes (Thermo Fisher Scientific), and then the PCR product was ligated with the vector with the use of T4 DNA ligase (Thermo Fisher Scientific). .. The resulting construct, inserted into a pLEV113 expression vector, was used to stably transfect CHO-S cells. scFv-Fc fusion proteins preceded with a secretion signal peptide were expressed and purified using affinity chromatography following the procedure described for the ECD_FGFR-Fc proteins [ ].

    Mutagenesis:

    Article Title: Controller protein of restriction–modification system Kpn2I affects transcription of its gene by acting as a transcription elongation roadblock
    Article Snippet: .. Transcription reactions were set in 10 μl and 40 nM of transcription templates with kpn 2I promoters, gal P1 , or T7 A1 ( ) and 100 nM E. coli RNAP σ70 holoenzyme (or its mutant versions) in a buffer containing 40 mM Tris–HCl, pH 8.0, 40 mM KCl, 10 mM MgCl2 , 1 mM DTT, 50 μg BSA, 5% glycerol, 10 U RiboLock RNase Inhibitor (Thermo Scientific). ..

    Construct:

    Article Title: An efficient procedure for purification of recombinant human ? heat shock protein 90
    Article Snippet: .. The recombinant plasmid was constructed by using the Kpn2 I and BstE II restriction enzymes (Fermentas, Lituvania). .. Expression, primary purification and analysis of the human Hsp90β subunit The Nova Blue strain of E. coli was transformed using above construct and protein expression was performed in LB (Lorian Bertani) medium containing 15µg/ml of kanamycine (Fermentas, Lituvania) ( , ).

    Purification:

    Article Title: Engineering TATA-binding protein Spt15 to improve ethanol tolerance and production in Kluyveromyces marxianus
    Article Snippet: .. Then the pooled PCR products were purified using EasyPure PCR Purification Kit (TransGen Biotech, Beijing, China) and digested overnight at 37 °C using Kpn I and Kpn 2I (ThermoFisher Scientific, Waltham, MA, USA). .. The vector pKmLP2-SPT15 was also digested with the same restriction enzymes and the pKmLP2 backbone was purified using EasyPure Quick Gel Extraction Kit (TransGen Biotech, Beijing, China) after agarose gel electrophoresis.

    Article Title: Engineering TATA-binding protein Spt15 to improve ethanol tolerance and production in Kluyveromyces marxianus
    Article Snippet: .. Then the pooled PCR products were purified using EasyPure PCR Purification Kit (TransGen Biotech, Beijing, China) and digested overnight at 37 °C using Kpn I and Kpn 2I (ThermoFisher Scientific, Waltham, MA, USA). .. The vector pKmLP2- SPT15 was also digested with the same restriction enzymes and the pKmLP2 backbone was purified using EasyPure Quick Gel Extraction Kit (TransGen Biotech, Beijing, China) after agarose gel electrophoresis.

    Polymerase Chain Reaction:

    Article Title: Specific Antibody Fragment Ligand Traps Blocking FGF1 Activity
    Article Snippet: .. In the next step, amplified scFv DNA and pLEV113-Fc expression vector (LakePharma, Belmont, CA, USA), encoding the Fc domain of human IgG1 [ ], were digested using HindIII and Kpn2I restriction enzymes (Thermo Fisher Scientific), and then the PCR product was ligated with the vector with the use of T4 DNA ligase (Thermo Fisher Scientific). .. The resulting construct, inserted into a pLEV113 expression vector, was used to stably transfect CHO-S cells. scFv-Fc fusion proteins preceded with a secretion signal peptide were expressed and purified using affinity chromatography following the procedure described for the ECD_FGFR-Fc proteins [ ].

    Article Title: Engineering TATA-binding protein Spt15 to improve ethanol tolerance and production in Kluyveromyces marxianus
    Article Snippet: .. Then the pooled PCR products were purified using EasyPure PCR Purification Kit (TransGen Biotech, Beijing, China) and digested overnight at 37 °C using Kpn I and Kpn 2I (ThermoFisher Scientific, Waltham, MA, USA). .. The vector pKmLP2-SPT15 was also digested with the same restriction enzymes and the pKmLP2 backbone was purified using EasyPure Quick Gel Extraction Kit (TransGen Biotech, Beijing, China) after agarose gel electrophoresis.

    Article Title: Engineering TATA-binding protein Spt15 to improve ethanol tolerance and production in Kluyveromyces marxianus
    Article Snippet: .. Then the pooled PCR products were purified using EasyPure PCR Purification Kit (TransGen Biotech, Beijing, China) and digested overnight at 37 °C using Kpn I and Kpn 2I (ThermoFisher Scientific, Waltham, MA, USA). .. The vector pKmLP2- SPT15 was also digested with the same restriction enzymes and the pKmLP2 backbone was purified using EasyPure Quick Gel Extraction Kit (TransGen Biotech, Beijing, China) after agarose gel electrophoresis.

    Expressing:

    Article Title: Specific Antibody Fragment Ligand Traps Blocking FGF1 Activity
    Article Snippet: .. In the next step, amplified scFv DNA and pLEV113-Fc expression vector (LakePharma, Belmont, CA, USA), encoding the Fc domain of human IgG1 [ ], were digested using HindIII and Kpn2I restriction enzymes (Thermo Fisher Scientific), and then the PCR product was ligated with the vector with the use of T4 DNA ligase (Thermo Fisher Scientific). .. The resulting construct, inserted into a pLEV113 expression vector, was used to stably transfect CHO-S cells. scFv-Fc fusion proteins preceded with a secretion signal peptide were expressed and purified using affinity chromatography following the procedure described for the ECD_FGFR-Fc proteins [ ].

    Sequencing:

    Article Title: Combined strategies for improving expression of Citrobacter amalonaticus phytase in Pichia pastoris
    Article Snippet: .. Yeast transformation Plasmids HKA, Phy, AOXm, SP-D, αE10, α∆57-70, 2c, 4c, and 6c were linearized with Kpn 2I (Thermo Scientific), whose cleavage site was located in the his4 sequence, and transformed into P. pastoris GS115 competent cells using the standard lithium chloride transformation method according to the manufacturer’s protocol (Invitrogen). ..

    Transformation Assay:

    Article Title: Combined strategies for improving expression of Citrobacter amalonaticus phytase in Pichia pastoris
    Article Snippet: .. Yeast transformation Plasmids HKA, Phy, AOXm, SP-D, αE10, α∆57-70, 2c, 4c, and 6c were linearized with Kpn 2I (Thermo Scientific), whose cleavage site was located in the his4 sequence, and transformed into P. pastoris GS115 competent cells using the standard lithium chloride transformation method according to the manufacturer’s protocol (Invitrogen). ..

    Article Title: Fhl1p protein, a positive transcription factor in Pichia pastoris, enhances the expression of recombinant proteins
    Article Snippet: .. Yeast transformation and regeneration of selectable markers The plasmid pmRFP was linearized with Kpn 2I (Thermo Scientific, Waltham, MA) and transformed into GS115 competent cells, creating GS115/mRFP (mRFP). ..

    Recombinant:

    Article Title: An efficient procedure for purification of recombinant human ? heat shock protein 90
    Article Snippet: .. The recombinant plasmid was constructed by using the Kpn2 I and BstE II restriction enzymes (Fermentas, Lituvania). .. Expression, primary purification and analysis of the human Hsp90β subunit The Nova Blue strain of E. coli was transformed using above construct and protein expression was performed in LB (Lorian Bertani) medium containing 15µg/ml of kanamycine (Fermentas, Lituvania) ( , ).

    Plasmid Preparation:

    Article Title: Specific Antibody Fragment Ligand Traps Blocking FGF1 Activity
    Article Snippet: .. In the next step, amplified scFv DNA and pLEV113-Fc expression vector (LakePharma, Belmont, CA, USA), encoding the Fc domain of human IgG1 [ ], were digested using HindIII and Kpn2I restriction enzymes (Thermo Fisher Scientific), and then the PCR product was ligated with the vector with the use of T4 DNA ligase (Thermo Fisher Scientific). .. The resulting construct, inserted into a pLEV113 expression vector, was used to stably transfect CHO-S cells. scFv-Fc fusion proteins preceded with a secretion signal peptide were expressed and purified using affinity chromatography following the procedure described for the ECD_FGFR-Fc proteins [ ].

    Article Title: An efficient procedure for purification of recombinant human ? heat shock protein 90
    Article Snippet: .. The recombinant plasmid was constructed by using the Kpn2 I and BstE II restriction enzymes (Fermentas, Lituvania). .. Expression, primary purification and analysis of the human Hsp90β subunit The Nova Blue strain of E. coli was transformed using above construct and protein expression was performed in LB (Lorian Bertani) medium containing 15µg/ml of kanamycine (Fermentas, Lituvania) ( , ).

    Article Title: Fhl1p protein, a positive transcription factor in Pichia pastoris, enhances the expression of recombinant proteins
    Article Snippet: .. Yeast transformation and regeneration of selectable markers The plasmid pmRFP was linearized with Kpn 2I (Thermo Scientific, Waltham, MA) and transformed into GS115 competent cells, creating GS115/mRFP (mRFP). ..

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  • 93
    Thermo Fisher kpn 2i promoters
    Kpn 2i Promoters, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kpn 2i promoters/product/Thermo Fisher
    Average 93 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    kpn 2i promoters - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

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