kodxtreme taq polymerase  (Millipore)


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    Structured Review

    Millipore kodxtreme taq polymerase
    Kodxtreme Taq Polymerase, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kodxtreme taq polymerase/product/Millipore
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    kodxtreme taq polymerase - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Negative Control:

    Article Title: SMAD2 Inactivation Inhibits CLDN6 Methylation to Suppress Migration and Invasion of Breast Cancer Cells
    Article Snippet: .. Antibodies included anti-DNMT1 (1:500, Cell Signaling Technology), anti-IgG (Millipore), and anti-DNA polymerase II (Millipore); anti-IgG was the negative control and anti-DNA polymerase II was the positive control. .. Transfection With Short Hairpin RNA Cells were transfected with short hairpin RNA (shRNA) by using Lipofectamine 2000 (Invitrogen), following the procedure recommended by the manufacturer. shRNA targeting of CLDN6 (5’-GTGCAAGGTGTACGACTCA-3’) and a negative control shRNA were purchased from GeneChem Co. Ltd.

    Amplification:

    Article Title: Molecular Characterization of Natural Erwinia pyrifoliae Strains Deficient in Hypersensitive Response
    Article Snippet: .. To reconfirm the hrpL sequences, DNA from strains Ep2/97, Ep4/97, and Ep16/96 was also amplified with a proofreading DNA polymerase (Accu Taq ; Sigma). .. The same sequence as in Fig. was obtained for the three wild-type strains, and the change from C to T at position 277 for strain Ep2/97 was observed.

    Positive Control:

    Article Title: SMAD2 Inactivation Inhibits CLDN6 Methylation to Suppress Migration and Invasion of Breast Cancer Cells
    Article Snippet: .. Antibodies included anti-DNMT1 (1:500, Cell Signaling Technology), anti-IgG (Millipore), and anti-DNA polymerase II (Millipore); anti-IgG was the negative control and anti-DNA polymerase II was the positive control. .. Transfection With Short Hairpin RNA Cells were transfected with short hairpin RNA (shRNA) by using Lipofectamine 2000 (Invitrogen), following the procedure recommended by the manufacturer. shRNA targeting of CLDN6 (5’-GTGCAAGGTGTACGACTCA-3’) and a negative control shRNA were purchased from GeneChem Co. Ltd.

    Labeling:

    Article Title: E2F1 Regulates the Base Excision Repair Gene XRCC1 and Promotes DNA Repair *
    Article Snippet: .. Cells were rinsed in phosphate-buffered saline, permeabilized with 1% Triton X-100 for 20 min, and then incubated in a moist air chamber at 37 °C for 90 min in a labeling mixture containing 10 μ m each dGTP, dATP, and dCTP and 7 μ m dTTP, 3 μ m FITC-dUTP, 20 units/ml Escherichia coli DNA polymerase I (Sigma) in reaction buffer containing 5 m m MgCl2 , 10 m m 2-mercaptoethanol, and 20 μg/ml bovine serum albumin. ..

    Concentration Assay:

    Article Title: The Heme Metabolite Carbon Monoxide Facilitates KSHV Infection by Inhibiting TLR4 Signaling in Endothelial Cells
    Article Snippet: .. The viral DNA polymerase inhibitor Foscarnet (FOS, Sigma) was used at a concentration of 400 μM. .. The CO releasing molecule CORM-2 (Sigma) was used at a concentration of 10 μM.

    Incubation:

    Article Title: E2F1 Regulates the Base Excision Repair Gene XRCC1 and Promotes DNA Repair *
    Article Snippet: .. Cells were rinsed in phosphate-buffered saline, permeabilized with 1% Triton X-100 for 20 min, and then incubated in a moist air chamber at 37 °C for 90 min in a labeling mixture containing 10 μ m each dGTP, dATP, and dCTP and 7 μ m dTTP, 3 μ m FITC-dUTP, 20 units/ml Escherichia coli DNA polymerase I (Sigma) in reaction buffer containing 5 m m MgCl2 , 10 m m 2-mercaptoethanol, and 20 μg/ml bovine serum albumin. ..

    Activity Assay:

    Article Title: Characterisation of Muta(TM)Mouse ?gt10-lacZ transgene: evidence for in vivo rearrangements
    Article Snippet: .. These custom-made primers (Cortec DNA Service Laboratories Inc., Kingston, Canada) were used to generate polymerase chain reaction (PCR) fragments in reactions involving a DNA polymerase deficient in 3′–5′ exonuclease activity (KOD polymerase; Novagen, Gibbstown, NJ, USA) to minimise proofreading errors. .. Fragments generated, either by PCR or cloning in E.coli , were sequenced in both directions using an ABI Prism® 3100 Genetic Analyzer and BigDye® Terminator Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA).

    Polymerase Chain Reaction:

    Article Title: Characterisation of Muta(TM)Mouse ?gt10-lacZ transgene: evidence for in vivo rearrangements
    Article Snippet: .. These custom-made primers (Cortec DNA Service Laboratories Inc., Kingston, Canada) were used to generate polymerase chain reaction (PCR) fragments in reactions involving a DNA polymerase deficient in 3′–5′ exonuclease activity (KOD polymerase; Novagen, Gibbstown, NJ, USA) to minimise proofreading errors. .. Fragments generated, either by PCR or cloning in E.coli , were sequenced in both directions using an ABI Prism® 3100 Genetic Analyzer and BigDye® Terminator Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA).

    Article Title: Promoting the accumulation of scopolamine and hyoscyamine in Hyoscyamus niger L. through EMS based mutagenesis
    Article Snippet: .. PCR amplifications were carried out in a reaction volume of 50μl containing 50–100 ng of genomic DNA, 0.2 mM of each dNTP, 0.1μM of each primer, and 0.01 units of Taq/μl DNA polymerase (Sigma) in 1x Taq Buffer containing Tris-HCl, KCl, (NH4 )2 SO4 , 1.5mM MgCl2 ; pH 8.7. .. Amplification was done using PCR program as denaturation at 94οC for 10 min, followed by denaturation for 30 seconds, annealing at 45°C (H6H gene) and 54°C (PMT gene), extension at 72°C for 1 minute and a final extension at 72°C for 5 minutes.

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  • 99
    Millipore kodxtreme taq polymerase
    Kodxtreme Taq Polymerase, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kodxtreme taq polymerase/product/Millipore
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    kodxtreme taq polymerase - by Bioz Stars, 2020-08
    99/100 stars
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