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Illumina Inc klenow dna polymerase
Klenow Dna Polymerase, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 93/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/klenow dna polymerase/product/Illumina Inc
Average 93 stars, based on 33 article reviews
Price from $9.99 to $1999.99
klenow dna polymerase - by Bioz Stars, 2020-07
93/100 stars

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DNA Ligation:

Article Title: Unique Epigenetic Programming Distinguishes Regenerative Spermatogonial Stem Cells in the Developing Mouse Testis
Article Snippet: .. In brief, samples were end-repaired in 1× T4 DNA ligase buffer, 0.4 mM dNTP mix, 2.25U T4 DNA polymerase, 0.75U Klenow DNA polymerase and 7.5U T4 polynucleotide kinase for 30 min at 21-25°C, then A-tailed in 1× NEB buffer 2, 0.4 mM dNTPs and 3.75U of Klenow(exo-) for 30 min at 37°C and then ligated in 1× rapid DNA ligation buffer plus 1mM Illumina PE adapters and 1,600U DNA ligase for 1-8h at 21-25°C. .. Ligated fragments were amplified using dual-indexed primers for Illumina (NEB #E7600S) for 8-10 PCR cycles.

Ligation:

Article Title: T cell epigenetic remodeling and accelerated epigenetic aging are linked to long-term immune alterations in childhood cancer survivors
Article Snippet: .. Genomic DNA was fragmented by overnight incubation with Msp1 restriction enzyme and adenylated with dATP and Klenow DNA polymerase (3′ to 5′ exo minus) before ligation to TruSeq adapters (Illumina, CA, USA). .. Twelve ligated samples were pooled and processed for bisulfite conversion using EZ DNA methylation kit (Zymo Research, CA, USA).

Article Title: Loss of the Notch effector RBPJ promotes tumorigenesis
Article Snippet: .. This involved DNA end-repair, and phosphorylation by T4 DNA polymerase, Klenow DNA Polymerase, and T4 polynucleotide kinase, respectively, in a single reaction and subsequent 3′ A overhang generation by Klenow fragment (3′ to 5′ exo minus) and ligation to Illumina SE adapters (with 5′ overhangs). .. Adapter-ligated products were purified on Qiaquick spin columns (QIAGEN) and PCR-amplified using Phusion DNA polymerase for 10 cycles and the SE primer set (Illumina).

Article Title: Distribution, recognition and regulation of non-CpG methylation in the adult mammalian brain
Article Snippet: .. These cDNA fragments went through an end-repair process using T4 DNA polymerase, T4 PNK and Klenow DNA polymerase, and the addition of a single ‘A’ base using Klenow Fragment (3' to 5' exo minus), then the ligation of the Illumina PE adapters using T4 DNA ligase. .. An index (up to 12) was inserted into Illumina adapters so that multiple samples can be sequenced in one lane of 8-lane flow cell if necessary.

Article Title: Lack of adrenomedullin in mouse endothelial cells results in defective angiogenesis, enhanced vascular permeability, less metastasis, and more brain damage
Article Snippet: .. The cDNA products were incubated with Klenow DNA polymerase to generate 3′ Adenine overhangs, therefore allowing ligation to Illumina indexing adapters to the double stranded cDNA ends. .. The adapter-ligated products were purified with Ampure XP magnetic beads (Agencourt Bioscience Corporation, Beverly, MA, USA) and libraries were amplified by 15 cycles of PCR with Phusion DNA polymerase (Finnzymes Reagents, Vantaa, Finland).

Purification:

Article Title: Comparison of Clinical Targeted Next-Generation Sequence Data from Formalin-Fixed and Fresh-Frozen Tissue Specimens
Article Snippet: .. Fragmentation was verified on an Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA), and the fragmented DNA was purified with Agencourt AmpureXP beads (Beckman Coulter, Danvers, MA), end-repaired and A-tailed with Klenow DNA polymerase, and finally ligated to universal Illumina adapters. .. Library fragments were then bead purified and analyzed for adequate ligation on an Agilent 2100 bioanalyzer (Agilent, Santa Clara, CA).

Modification:

Article Title: Gene expression profiling of oxidative stress response of C. elegans aging defective AMPK mutants using massively parallel transcriptome sequencing
Article Snippet: .. The gel-purified cDNA products were modified for sequencing using the genomic DNA prep kit (FC-102-1002, Illumina) as follows: Size-selected cDNAs were subject to end-repair, 3' A overhangs generation, and phosphorylation by T4 DNA polymerase, Klenow DNA Polymerase, and T4 polynucleotide kinase respectively in a single reaction, and then ligated to Illumina adapters, which contain 5' T overhangs. .. The adapter-ligated products were purified on Qiaquick spin columns (Qiagen), then PCR-amplified with Phusion DNA Polymerase in 10 cycles using Illumina's genomic DNA primer set (Illumina).

Incubation:

Article Title: T cell epigenetic remodeling and accelerated epigenetic aging are linked to long-term immune alterations in childhood cancer survivors
Article Snippet: .. Genomic DNA was fragmented by overnight incubation with Msp1 restriction enzyme and adenylated with dATP and Klenow DNA polymerase (3′ to 5′ exo minus) before ligation to TruSeq adapters (Illumina, CA, USA). .. Twelve ligated samples were pooled and processed for bisulfite conversion using EZ DNA methylation kit (Zymo Research, CA, USA).

Article Title: Lack of adrenomedullin in mouse endothelial cells results in defective angiogenesis, enhanced vascular permeability, less metastasis, and more brain damage
Article Snippet: .. The cDNA products were incubated with Klenow DNA polymerase to generate 3′ Adenine overhangs, therefore allowing ligation to Illumina indexing adapters to the double stranded cDNA ends. .. The adapter-ligated products were purified with Ampure XP magnetic beads (Agencourt Bioscience Corporation, Beverly, MA, USA) and libraries were amplified by 15 cycles of PCR with Phusion DNA polymerase (Finnzymes Reagents, Vantaa, Finland).

Sequencing:

Article Title: Gene expression profiling of oxidative stress response of C. elegans aging defective AMPK mutants using massively parallel transcriptome sequencing
Article Snippet: .. The gel-purified cDNA products were modified for sequencing using the genomic DNA prep kit (FC-102-1002, Illumina) as follows: Size-selected cDNAs were subject to end-repair, 3' A overhangs generation, and phosphorylation by T4 DNA polymerase, Klenow DNA Polymerase, and T4 polynucleotide kinase respectively in a single reaction, and then ligated to Illumina adapters, which contain 5' T overhangs. .. The adapter-ligated products were purified on Qiaquick spin columns (Qiagen), then PCR-amplified with Phusion DNA Polymerase in 10 cycles using Illumina's genomic DNA primer set (Illumina).

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    Illumina Inc dna polymerase i
    Dna Polymerase I, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 93/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna polymerase i/product/Illumina Inc
    Average 93 stars, based on 67 article reviews
    Price from $9.99 to $1999.99
    dna polymerase i - by Bioz Stars, 2020-07
    93/100 stars
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