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GE Healthcare klenow dna polymerase
Klenow Dna Polymerase, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/klenow dna polymerase/product/GE Healthcare
Average 92 stars, based on 11 article reviews
Price from $9.99 to $1999.99
klenow dna polymerase - by Bioz Stars, 2020-07
92/100 stars

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Synthesized:

Article Title: Redox Regulation of an AP-1-Like Transcription Factor, YapA, in the Fungal Symbiont Epichloë festucae
Article Snippet: .. The DNA probes were synthesized by random priming with Klenow DNA polymerase and [α-32 P]dCTP (3,000 Ci/mmol; Amersham Biosciences), using a High Prime DNA labeling kit (Roche), or by the incorporation of digoxigenin-11-dUTP (DIG-11-dUTP) into DNA by PCR, using a PCR DIG probe synthesis kit (Roche). .. Hybridizations were performed according to the manufacturer's instructions and visualized by either autoradiography (32 P-labeled probes) or nitroblue tetrazolium–5-bromo-4-chloro-3-indolylphosphate (NBT-BCIP) color detection (DIG-labeled probes).

Northern Blot:

Article Title:
Article Snippet: .. Northern blots were performed as described previously ( , ) with the following modifications: (i) membrane prehybridization and hybridization were performed in Church buffer at 65 °C; (ii) samples were separated by electrophoresis on 1% agarose gels containing formaldehyde; (iii) PCR-labeled probes were obtained by incorporating [α-32 P]dCTP with Klenow DNA polymerase using the Megaprime DNA labeling system according to the manufacturer's instructions (Amersham Biosciences); and (iv) the PCR products were purified using the SigmaSpin Post-Reaction Clean-up Columns according to manufacturer's instructions (Sigma). .. Probes for Northern blot analysis were ∼200 bp in length.

Labeling:

Article Title: The Wide-Domain Carbon Catabolite Repressor CreA Indirectly Controls Expression of the Aspergillus nidulans xlnB Gene, Encoding the Acidic Endo-?-(1,4)-Xylanase X24 †
Article Snippet: .. Fragments 1B (−647 to −372; positions are given relative to the ATG codon), 2B (−448 to −340), 3B (−372 to −133), 4B (−174 to +5), 5B and m5B (−633 to −497), and 6B and m6B (−328 to −79) were end labeled by filling the protruding ends using Klenow DNA polymerase in the presence of [α32 P]dCTP (3,000 Ci/mmol; Amersham). ..

Article Title: Nucleosome occupancy reveals regulatory elements of the CFTR promoter
Article Snippet: .. Electromobility shift assays Complementary single-stranded oligonucleotides ( and Supplementary Table S1 for sequences) were annealed and labeled with [α-32 P]-dCTP by fill-in reactions with Klenow DNA polymerase, prior to purification with microspin G-25 columns (Amersham Biosciences). .. Labeled DNA probes were incubated for 15 min with 5 µg nuclear extract in a final reaction volume of 20 µl containing 20% (v/v) glycerol, 20 mM HEPES pH 8.0, 4 mM MgCl2 , 100 mM KCl, 32 mM NaCl, 0.4 µg/µl bovine serum albumin (BSA), 20 mM DTT and 0.05 µg/µl poly(dI–dC).

Purification:

Article Title: Nucleosome occupancy reveals regulatory elements of the CFTR promoter
Article Snippet: .. Electromobility shift assays Complementary single-stranded oligonucleotides ( and Supplementary Table S1 for sequences) were annealed and labeled with [α-32 P]-dCTP by fill-in reactions with Klenow DNA polymerase, prior to purification with microspin G-25 columns (Amersham Biosciences). .. Labeled DNA probes were incubated for 15 min with 5 µg nuclear extract in a final reaction volume of 20 µl containing 20% (v/v) glycerol, 20 mM HEPES pH 8.0, 4 mM MgCl2 , 100 mM KCl, 32 mM NaCl, 0.4 µg/µl bovine serum albumin (BSA), 20 mM DTT and 0.05 µg/µl poly(dI–dC).

Article Title:
Article Snippet: .. Northern blots were performed as described previously ( , ) with the following modifications: (i) membrane prehybridization and hybridization were performed in Church buffer at 65 °C; (ii) samples were separated by electrophoresis on 1% agarose gels containing formaldehyde; (iii) PCR-labeled probes were obtained by incorporating [α-32 P]dCTP with Klenow DNA polymerase using the Megaprime DNA labeling system according to the manufacturer's instructions (Amersham Biosciences); and (iv) the PCR products were purified using the SigmaSpin Post-Reaction Clean-up Columns according to manufacturer's instructions (Sigma). .. Probes for Northern blot analysis were ∼200 bp in length.

Electrophoresis:

Article Title:
Article Snippet: .. Northern blots were performed as described previously ( , ) with the following modifications: (i) membrane prehybridization and hybridization were performed in Church buffer at 65 °C; (ii) samples were separated by electrophoresis on 1% agarose gels containing formaldehyde; (iii) PCR-labeled probes were obtained by incorporating [α-32 P]dCTP with Klenow DNA polymerase using the Megaprime DNA labeling system according to the manufacturer's instructions (Amersham Biosciences); and (iv) the PCR products were purified using the SigmaSpin Post-Reaction Clean-up Columns according to manufacturer's instructions (Sigma). .. Probes for Northern blot analysis were ∼200 bp in length.

DNA Labeling:

Article Title: Redox Regulation of an AP-1-Like Transcription Factor, YapA, in the Fungal Symbiont Epichloë festucae
Article Snippet: .. The DNA probes were synthesized by random priming with Klenow DNA polymerase and [α-32 P]dCTP (3,000 Ci/mmol; Amersham Biosciences), using a High Prime DNA labeling kit (Roche), or by the incorporation of digoxigenin-11-dUTP (DIG-11-dUTP) into DNA by PCR, using a PCR DIG probe synthesis kit (Roche). .. Hybridizations were performed according to the manufacturer's instructions and visualized by either autoradiography (32 P-labeled probes) or nitroblue tetrazolium–5-bromo-4-chloro-3-indolylphosphate (NBT-BCIP) color detection (DIG-labeled probes).

Article Title:
Article Snippet: .. Northern blots were performed as described previously ( , ) with the following modifications: (i) membrane prehybridization and hybridization were performed in Church buffer at 65 °C; (ii) samples were separated by electrophoresis on 1% agarose gels containing formaldehyde; (iii) PCR-labeled probes were obtained by incorporating [α-32 P]dCTP with Klenow DNA polymerase using the Megaprime DNA labeling system according to the manufacturer's instructions (Amersham Biosciences); and (iv) the PCR products were purified using the SigmaSpin Post-Reaction Clean-up Columns according to manufacturer's instructions (Sigma). .. Probes for Northern blot analysis were ∼200 bp in length.

Polymerase Chain Reaction:

Article Title: Redox Regulation of an AP-1-Like Transcription Factor, YapA, in the Fungal Symbiont Epichloë festucae
Article Snippet: .. The DNA probes were synthesized by random priming with Klenow DNA polymerase and [α-32 P]dCTP (3,000 Ci/mmol; Amersham Biosciences), using a High Prime DNA labeling kit (Roche), or by the incorporation of digoxigenin-11-dUTP (DIG-11-dUTP) into DNA by PCR, using a PCR DIG probe synthesis kit (Roche). .. Hybridizations were performed according to the manufacturer's instructions and visualized by either autoradiography (32 P-labeled probes) or nitroblue tetrazolium–5-bromo-4-chloro-3-indolylphosphate (NBT-BCIP) color detection (DIG-labeled probes).

Article Title:
Article Snippet: .. Northern blots were performed as described previously ( , ) with the following modifications: (i) membrane prehybridization and hybridization were performed in Church buffer at 65 °C; (ii) samples were separated by electrophoresis on 1% agarose gels containing formaldehyde; (iii) PCR-labeled probes were obtained by incorporating [α-32 P]dCTP with Klenow DNA polymerase using the Megaprime DNA labeling system according to the manufacturer's instructions (Amersham Biosciences); and (iv) the PCR products were purified using the SigmaSpin Post-Reaction Clean-up Columns according to manufacturer's instructions (Sigma). .. Probes for Northern blot analysis were ∼200 bp in length.

Hybridization:

Article Title:
Article Snippet: .. Northern blots were performed as described previously ( , ) with the following modifications: (i) membrane prehybridization and hybridization were performed in Church buffer at 65 °C; (ii) samples were separated by electrophoresis on 1% agarose gels containing formaldehyde; (iii) PCR-labeled probes were obtained by incorporating [α-32 P]dCTP with Klenow DNA polymerase using the Megaprime DNA labeling system according to the manufacturer's instructions (Amersham Biosciences); and (iv) the PCR products were purified using the SigmaSpin Post-Reaction Clean-up Columns according to manufacturer's instructions (Sigma). .. Probes for Northern blot analysis were ∼200 bp in length.

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  • 92
    GE Healthcare klenow polymerase
    Klenow Polymerase, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/klenow polymerase/product/GE Healthcare
    Average 92 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    klenow polymerase - by Bioz Stars, 2020-07
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    92
    GE Healthcare klenow fragment
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    GE Healthcare klenow fragment polymerase labeling
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