klebsiella pneumoniae atcc 700603  (ATCC)

Journal: BMC Microbiology

Article Title: Isolation, characterization, and genomic analysis of novel bacteriophage AEV23 against multidrug-resistant Klebsiella pneumoniae isolated from Cystic Fibrosis patients

doi: 10.1186/s12866-025-04243-6

Figure Lengend Snippet: MOIs of AEV23 phage (0.1, 1 and 10) for K. pneumoniae . The phage exhibited similar activity at MOIs of 0.1, 1, and 10, but the highest progeny was observed at MOI = 1. Data are shown as the mean ± SD from the triplicate experiments. The asterisks (*** P < 0.001, ** P < 0.01 or * P < 0.05) indicate a significant difference between each phage-treated group and the control with no phage treatment and, the ns shows non-significant difference

Article Snippet: The lytic activity of the phage suspension was evaluated against 9 clinical isolates of K. pneumoniae (1.5 × 108 CFU/mL), 2 Pseudomonas aeruginosa isolates (1.5 × 108 CFU/mL), 2 Staphylococcus aureus isolates (1.5 × 108 CFU/mL) from Cystic Fibrosis patients, and one standard strain of K. pneumoniae (ATCC 700603) using the spot assay.

Techniques: Activity Assay, Control

Journal: BMC Microbiology

Article Title: Isolation, characterization, and genomic analysis of novel bacteriophage AEV23 against multidrug-resistant Klebsiella pneumoniae isolated from Cystic Fibrosis patients

doi: 10.1186/s12866-025-04243-6

Figure Lengend Snippet: Adsorption ( A ) and One-step growth curve of phage ( B ) in K. pneumoniae . ( A ) The absorbed bacteriophage titration showed that 92% of the isolated phages were absorbed by bacterial cells in 5 min. ( B ) The one-step growth experiment revealed a latent period of 30 min and a rise period of 40 min. The burst size was approximately 98 phage particles per infected bacterium. The bars represent the mean ± SD from the triplicate experiments

Article Snippet: The lytic activity of the phage suspension was evaluated against 9 clinical isolates of K. pneumoniae (1.5 × 108 CFU/mL), 2 Pseudomonas aeruginosa isolates (1.5 × 108 CFU/mL), 2 Staphylococcus aureus isolates (1.5 × 108 CFU/mL) from Cystic Fibrosis patients, and one standard strain of K. pneumoniae (ATCC 700603) using the spot assay.

Techniques: Adsorption, Titration, Isolation, Infection

Journal: BMC Microbiology

Article Title: Isolation, characterization, and genomic analysis of novel bacteriophage AEV23 against multidrug-resistant Klebsiella pneumoniae isolated from Cystic Fibrosis patients

doi: 10.1186/s12866-025-04243-6

Figure Lengend Snippet: Phage biofilm removal assay. ( A ) The highest biofilm removal of K. pneumoniae isolate 5 was observed at a dilution of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\:{10}^{-2}$$\end{document} ( B ) The highest biofilm removal of K. pneumoniae standard strain was observed at a dilution of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\:{10}^{-1}$$\end{document} . Data are shown as the mean ± SD from the triplicate experiments. The asterisks (*** P < 0.001, ** P < 0.01 or * P < 0.05) indicate a significant difference between each phage-treated group and the control with no phage treatment, and the ns shows a non-significant difference

Article Snippet: The lytic activity of the phage suspension was evaluated against 9 clinical isolates of K. pneumoniae (1.5 × 108 CFU/mL), 2 Pseudomonas aeruginosa isolates (1.5 × 108 CFU/mL), 2 Staphylococcus aureus isolates (1.5 × 108 CFU/mL) from Cystic Fibrosis patients, and one standard strain of K. pneumoniae (ATCC 700603) using the spot assay.

Techniques: Control

Journal: BMC Microbiology

Article Title: Isolation, characterization, and genomic analysis of novel bacteriophage AEV23 against multidrug-resistant Klebsiella pneumoniae isolated from Cystic Fibrosis patients

doi: 10.1186/s12866-025-04243-6

Figure Lengend Snippet: SEM micrograph of K. pneumoniae biofilm formation. K. pneumoniae formed biofilms on the cover glass. The scale of the left image is 2 μm, and that of the right image is 20 μm

Article Snippet: The lytic activity of the phage suspension was evaluated against 9 clinical isolates of K. pneumoniae (1.5 × 108 CFU/mL), 2 Pseudomonas aeruginosa isolates (1.5 × 108 CFU/mL), 2 Staphylococcus aureus isolates (1.5 × 108 CFU/mL) from Cystic Fibrosis patients, and one standard strain of K. pneumoniae (ATCC 700603) using the spot assay.

Techniques:

Journal: BMC Microbiology

Article Title: Isolation, characterization, and genomic analysis of novel bacteriophage AEV23 against multidrug-resistant Klebsiella pneumoniae isolated from Cystic Fibrosis patients

doi: 10.1186/s12866-025-04243-6

Figure Lengend Snippet: SEM micrograph of biofilm destruction by phage. The phages could destroy the biofilm formed by K. pneumoniae. The scale of the left image is 10 μm, the middle image is 2 μm, and the right image is 1 μm

Article Snippet: The lytic activity of the phage suspension was evaluated against 9 clinical isolates of K. pneumoniae (1.5 × 108 CFU/mL), 2 Pseudomonas aeruginosa isolates (1.5 × 108 CFU/mL), 2 Staphylococcus aureus isolates (1.5 × 108 CFU/mL) from Cystic Fibrosis patients, and one standard strain of K. pneumoniae (ATCC 700603) using the spot assay.

Techniques:

Journal: Scientific Reports

Article Title: Isolation and characterization of lytic bacteriophages with therapeutic potential against multidrug resistant Klebsiella pneumoniae from Ethiopia

doi: 10.1038/s41598-026-39153-8

Figure Lengend Snippet: Agarose gel electrophoresis analysis of the PCR products of K. pneumoniae phages. Lane M: DNA ladder (100 bp), lane NC: negative control, lanes with a number indicate sample lanes representing the PCR products of the corresponding genus-specific genes. “ A ” Putative tail tube-associated baseplate protein-encoding gene of the genus Taipeivirus (326 bp), “B” putative base plate gene of the genus Slopekvirus (411 bp), “ C ” capsid protein-encoding gene of the genus Drulisvirus (728 bp), “ D ” putative major head subunit precursor gene of the genus Webervirus (422 bp), “E” putative helicase gene of the genus Mydovirus (302 bp), “F” internal virion protein D of the genus Przondovirus (463 bp).

Article Snippet: Among the phages tested, 42.2% (43/102) demonstrated lytic activity against K. pneumoniae ATCC 700,603.

Techniques: Agarose Gel Electrophoresis, Negative Control