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Biotech AB kinase buffer
Kinase Buffer, supplied by Biotech AB, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/kinase buffer/product/Biotech AB
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
kinase buffer - by Bioz Stars, 2020-09
92/100 stars

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Related Articles

Incubation:

Article Title: Biochemical Characterization of the Tobacco 42-kD Protein Kinase Activated by Osmotic Stress 1Biochemical Characterization of the Tobacco 42-kD Protein Kinase Activated by Osmotic Stress 1 [w]
Article Snippet: .. Phosphorylation of protein and peptide substrates for NtOSAK kinetic studies: 10- μ L aliquots of purified enzyme (about 0.08 μ g) were incubated with one of the protein (MBP, casein, GST-ACC) or peptide ( SAMS , AMARA ) substrates in concentrations as indicated in “Results” and with 50 μ m ATP supplemented with 1 μ Ci [ γ 32 P]ATP (Amersham Pharmacia Biotech AB) in kinase buffer (25 m m β -glycerophosphate, pH 7.5, 1.25 m m EGTA, 1 m m DTT, 30 m m MgCl2 , and 150 μ m Na3 VO4 ). .. The final volume of an incubation sample was 25 μ L. After 10 min of incubation at 37°C, 20 μ L of the mixture was spotted onto a P81 phosphocellulose (in case of MBP and the peptides) or onto a 3 MM filter strip (in case of casein), and the reaction was terminated by washing the filter with 0.85% H3 PO4 or with 5% TCA, respectively.

Purification:

Article Title: Biochemical Characterization of the Tobacco 42-kD Protein Kinase Activated by Osmotic Stress 1Biochemical Characterization of the Tobacco 42-kD Protein Kinase Activated by Osmotic Stress 1 [w]
Article Snippet: .. Phosphorylation of protein and peptide substrates for NtOSAK kinetic studies: 10- μ L aliquots of purified enzyme (about 0.08 μ g) were incubated with one of the protein (MBP, casein, GST-ACC) or peptide ( SAMS , AMARA ) substrates in concentrations as indicated in “Results” and with 50 μ m ATP supplemented with 1 μ Ci [ γ 32 P]ATP (Amersham Pharmacia Biotech AB) in kinase buffer (25 m m β -glycerophosphate, pH 7.5, 1.25 m m EGTA, 1 m m DTT, 30 m m MgCl2 , and 150 μ m Na3 VO4 ). .. The final volume of an incubation sample was 25 μ L. After 10 min of incubation at 37°C, 20 μ L of the mixture was spotted onto a P81 phosphocellulose (in case of MBP and the peptides) or onto a 3 MM filter strip (in case of casein), and the reaction was terminated by washing the filter with 0.85% H3 PO4 or with 5% TCA, respectively.

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    Biotech AB kinase buffer
    Kinase Buffer, supplied by Biotech AB, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kinase buffer/product/Biotech AB
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    kinase buffer - by Bioz Stars, 2020-09
    92/100 stars
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