Journal: Biomedicines

Article Title: Echinacoside Alleviates Cisplatin-Induced Acute Kidney Injury by Regulating Bap1 to Inhibit Ferroptosis

doi: 10.3390/biomedicines14092127

Figure Lengend Snippet: ECH alleviates cisplatin-induced renal injury and inflammation in vivo. ( A ) Serum BUN levels in each group. ( B ) Serum creatinine levels of each group. ( C ) Serum KIM-1 levels detected by ELISA. ( D ) Serum NGAL levels detected by ELISA. ( E ) Representative H&E, PAS and KIM-1 immunohistochemical staining images of kidney tissues and quantitative analysis. ( F ) Western blot analysis and quantitative analysis of KIM-1 and NGAL protein in kidney tissues. ( G – J ) Relative mRNA levels of IL-1β, MCP-1, TNF-α and IL-6 in kidney tissues. ** p < 0.01, *** p < 0.001 vs. control group; # p < 0.05, ## p < 0.01 vs. cisplatin group. n = 3 mice per group.

Article Snippet: After membrane blocking, primary antibodies targeting KIM-1 (A24493, ABclonal, Wuhan, China), NGAL (A2092, ABclonal, Wuhan, China), GPX4 (A27995, ABclonal, Wuhan, China), Slc7a11 (A25302, ABclonal, Wuhan, China), Bap1 (A6533SP, ABclonal, Wuhan, China) and the internal reference GAPDH (A19056, ABclonal, Wuhan, China) were incubated overnight at 4 °C, followed by incubation with corresponding secondary antibodies.

Techniques: In Vivo, Enzyme-linked Immunosorbent Assay, Immunohistochemical staining, Staining, Analysis, Western Blot, Control

Journal: Biomedicines

Article Title: Echinacoside Alleviates Cisplatin-Induced Acute Kidney Injury by Regulating Bap1 to Inhibit Ferroptosis

doi: 10.3390/biomedicines14092127

Figure Lengend Snippet: ECH relieves cisplatin-induced cell damage in vitro. ( A ) HK-2 cell viability treated after treatment with different concentrations of ECH. ( B ) HK-2 cell viability under cisplatin stimulation with gradient ECH intervention. ( C ) Representative PI staining images of HK-2 cells and quantitative analysis. ( D ) Western blot and quantitative analysis of KIM-1 and NGAL proteins in HK-2 cells. ( E – H ) Relative mRNA levels of IL-1β, MCP-1, TNF-α and IL-6 in HK-2 cells. ns, no significancant; * p < 0.05, ** p < 0.01 vs. control group; # p < 0.05 vs. cisplatin group.

Article Snippet: After membrane blocking, primary antibodies targeting KIM-1 (A24493, ABclonal, Wuhan, China), NGAL (A2092, ABclonal, Wuhan, China), GPX4 (A27995, ABclonal, Wuhan, China), Slc7a11 (A25302, ABclonal, Wuhan, China), Bap1 (A6533SP, ABclonal, Wuhan, China) and the internal reference GAPDH (A19056, ABclonal, Wuhan, China) were incubated overnight at 4 °C, followed by incubation with corresponding secondary antibodies.

Techniques: In Vitro, Staining, Analysis, Western Blot, Control

Journal: Integrative Medicine Research

Article Title: Ameliorative effects of Wiryeongtang (herbal medicine) on cardiorenal complications via modulation of inflammatory and fibrotic responses in diabetic mice

doi: 10.1016/j.imr.2026.101322

Figure Lengend Snippet: The effects of WRT on renal morphology changes in db/db mice . (A) Kidney type and (B) Right/left kidney weight change. (C) Representative periodic acid-Schiff (PAS) staining of kidney section (5 mm, Magnification: 40×, scale bars:100 μm) and (D) Glomerular surface area and mesangial surface area. (E) Urinary KIM-1 measurement for indication of tubular injury. db/m, non-diabetic control mice; db/db, diabetic mice (untreated); vilda, vildagliptin-treated mice (positive control; db/db + vildagliptin); WRT, Wiryeongtang-treated mice (db/db + WRT). Data are expressed as mean±SD (n=5). **p<0.01 vs db/m group; #p<0.05, ##p<0.01 vs db/db group.

Article Snippet: Serum insulin (Shibyagi Co., Gunma, Japan) and KIM-1 (LSBIO., Ltd., Ishihara Shibukawa, Gunma, Japan) levels were measured using ELISA kits.

Techniques: Staining, Control, Positive Control