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GE Healthcare kd mwco protein concentrator
Kd Mwco Protein Concentrator, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/kd mwco protein concentrator/product/GE Healthcare
Average 89 stars, based on 1 article reviews
Price from $9.99 to $1999.99
kd mwco protein concentrator - by Bioz Stars, 2020-04
89/100 stars

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Centrifugation:

Article Title: Structure of the Varicella Zoster Virus Thymidylate Synthase Establishes Functional and Structural Similarities as the Human Enzyme and Potentiates Itself as a Target of Brivudine
Article Snippet: The cell lysate was sonicated before clarifying by centrifugation at 47000 g for 25 minutes at 4°C and filtration of the supernatant with an 1.2 μm syringe filter. .. The fractions were analyzed on a 4–12% NuPAGE Bis-Tris gel (Life Technologies) and the purest fractions were pooled and concentrated to 12 mg/mL with a 10 kD MWCO protein concentrator (GE Healthcare).

Filtration:

Article Title: Structure of the Varicella Zoster Virus Thymidylate Synthase Establishes Functional and Structural Similarities as the Human Enzyme and Potentiates Itself as a Target of Brivudine
Article Snippet: The samples eluted with 335 mM imidazole were pooled and concentrated to 5 mL before loading onto a pre-equilibrated (20 mM Na-HEPES pH 7.5, 300 mM NaCl, 10% glycerol and 0.5 mM TCEP) HiLoad 16/600 Superdex 200 gel filtration column (GE Healthcare). .. The fractions were analyzed on a 4–12% NuPAGE Bis-Tris gel (Life Technologies) and the purest fractions were pooled and concentrated to 12 mg/mL with a 10 kD MWCO protein concentrator (GE Healthcare).

Protease Inhibitor:

Article Title: Structure of the Varicella Zoster Virus Thymidylate Synthase Establishes Functional and Structural Similarities as the Human Enzyme and Potentiates Itself as a Target of Brivudine
Article Snippet: Ice-cold lysis buffer (100 mM Na-HEPES pH 8.0 (Sigma Aldrich), 500 mM NaCl (Sigma Aldrich), 10 mM imidazole pH 8.0 (Sigma Aldrich), 10% glycerol (Sigma Aldrich) and 0.5 mM TCEP (Sigma Aldrich)) supplemented with 0.1 mg/mL lysozyme (Sigma Aldrich), 1 μL/mL protease inhibitor cocktail (Nacalai) and 125 U/mL benzonase (Merck Millipore) was added to the frozen TSVZV cell pellet. .. The fractions were analyzed on a 4–12% NuPAGE Bis-Tris gel (Life Technologies) and the purest fractions were pooled and concentrated to 12 mg/mL with a 10 kD MWCO protein concentrator (GE Healthcare).

Purification:

Article Title: Structure of the Varicella Zoster Virus Thymidylate Synthase Establishes Functional and Structural Similarities as the Human Enzyme and Potentiates Itself as a Target of Brivudine
Article Snippet: The filtrate was loaded onto a pre-equilibrated (10 mM Na-HEPES pH 7.5, 500 mM NaCl, 10 mM imidazole pH 7.5, 10% glycerol and 0.5 mM TCEP) 5 mL HiTrap IMAC HP column (GE Healthcare) and the column was washed with 25 mL of purification wash buffer (10 mM Na-HEPES pH 7.5, 500 mM NaCl, 10 mM imidazole pH 7.5, 10% glycerol). .. The fractions were analyzed on a 4–12% NuPAGE Bis-Tris gel (Life Technologies) and the purest fractions were pooled and concentrated to 12 mg/mL with a 10 kD MWCO protein concentrator (GE Healthcare).

Protein Purification:

Article Title: Structure of the Varicella Zoster Virus Thymidylate Synthase Establishes Functional and Structural Similarities as the Human Enzyme and Potentiates Itself as a Target of Brivudine
Article Snippet: Paragraph title: Protein purification ... The fractions were analyzed on a 4–12% NuPAGE Bis-Tris gel (Life Technologies) and the purest fractions were pooled and concentrated to 12 mg/mL with a 10 kD MWCO protein concentrator (GE Healthcare).

Sonication:

Article Title: Structure of the Varicella Zoster Virus Thymidylate Synthase Establishes Functional and Structural Similarities as the Human Enzyme and Potentiates Itself as a Target of Brivudine
Article Snippet: The cell lysate was sonicated before clarifying by centrifugation at 47000 g for 25 minutes at 4°C and filtration of the supernatant with an 1.2 μm syringe filter. .. The fractions were analyzed on a 4–12% NuPAGE Bis-Tris gel (Life Technologies) and the purest fractions were pooled and concentrated to 12 mg/mL with a 10 kD MWCO protein concentrator (GE Healthcare).

Lysis:

Article Title: Structure of the Varicella Zoster Virus Thymidylate Synthase Establishes Functional and Structural Similarities as the Human Enzyme and Potentiates Itself as a Target of Brivudine
Article Snippet: Ice-cold lysis buffer (100 mM Na-HEPES pH 8.0 (Sigma Aldrich), 500 mM NaCl (Sigma Aldrich), 10 mM imidazole pH 8.0 (Sigma Aldrich), 10% glycerol (Sigma Aldrich) and 0.5 mM TCEP (Sigma Aldrich)) supplemented with 0.1 mg/mL lysozyme (Sigma Aldrich), 1 μL/mL protease inhibitor cocktail (Nacalai) and 125 U/mL benzonase (Merck Millipore) was added to the frozen TSVZV cell pellet. .. The fractions were analyzed on a 4–12% NuPAGE Bis-Tris gel (Life Technologies) and the purest fractions were pooled and concentrated to 12 mg/mL with a 10 kD MWCO protein concentrator (GE Healthcare).

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    GE Healthcare kd mwco protein concentrator
    Kd Mwco Protein Concentrator, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kd mwco protein concentrator/product/GE Healthcare
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    kd mwco protein concentrator - by Bioz Stars, 2020-04
    89/100 stars
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