kcl  (Worthington Biochemical)


Bioz Verified Symbol Worthington Biochemical is a verified supplier
Bioz Manufacturer Symbol Worthington Biochemical manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Worthington Biochemical kcl
    Kcl, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kcl/product/Worthington Biochemical
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    kcl - by Bioz Stars, 2021-03
    86/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Helical Structure of the Cooh Terminus of S3 and Its Contribution to the Gating Modifier Toxin Receptor in Voltage-Gated Ion Channels
    Article Snippet: Mutations were confirmed by automated DNA sequencing. cDNAs encoding wild-type and mutant channels were linearized with NotI and transcribed with T7 RNA polymerase. .. Oocytes from Xenopus laevis frogs were removed surgically and incubated with agitation for 1–1.5 h in a solution containing (in mM): 82.5 NaCl, 2.5 KCl, 1 MgCl2 , 5 HEPES, and 2 mg/ml collagenase (Worthington Biochemical Corp.), pH 7.6 with NaOH. .. Defolliculated Xenopus oocytes were injected with cRNA and incubated at 17°C in a solution containing (in mM): 96 NaCl, 2 KCl, 1 MgCl2 , 1.8 CaCl2 , 5 HEPES, and 50 μg/ml gentamicin (GIBCO BRL), pH 7.6 with NaOH for 1–4 d before recording.

    Article Title: Chromatin remodeling during in vivo neural stem cells differentiating to neurons in early Drosophila embryos
    Article Snippet: To examine bead binding, DAPI-stained total and affinity-purified nuclei were counted on a hemacytometer, respectively. .. MNase-seq and ChIP-seq Affinity-purified nuclei were pelleted by centrifugation at 1000 × g for 5 min and suspended with 500 μ l of 37 °C preheated MNase (micrococcal nuclease) digestion buffer (10 mM (pH 7.5) Tris-HCl, 15 mM NaCl, 60 mM KCl, 2 mM CaCl2 , 0.15 mM spermine, 0.5 mM spermidine, 1 × PI) with 12 U MNase (Worthington Biochemical Corporation, Lakewood, NJ, USA) and incubated at 37 °C for 20 min. .. The reaction was terminated on ice by adding EDTA to a final concentration of 10 mM for 10 min.

    Article Title: The ribbon-associated protein C-terminal-binding protein 1 is not essential for the structure and function of retinal ribbon synapses
    Article Snippet: .. Briefly, retinas were dissected from eyecups in standard solution, (NaCl 135 mM, KCl 5 mM, CaCl2 0.5 mM, MgCl2 1 mM, HEPES 10 mM, pH 7.4), treated for 25 min with hyaluronidase (500 units/ml; Worthington Biochemical, Lakewood, NJ), and then transferred to cold saline containing 2.7 mM DL-cysteine (Sigma-Aldrich, St. Louis, MO), cut into small pieces, and incubated 25–30 min in saline containing DL-cysteine and 15–30 units/ml papain (Sigma) at room temperature. .. A piece of retina was triturated via a flame-polished Pasteur pipette, and dissociated cells were plated onto glass-bottomed dishes containing saline with CaCl2 increased to 2.5 mM for patch-clamp recording.

    Article Title: Intrinsic versus extrinsic voltage sensitivity of blocker interaction with an ion channel pore
    Article Snippet: The cRNA was synthesized with T7 polymerase (Promega) using linearized cDNA as a template. .. Oocytes harvested from Xenopus laevis were incubated in a solution containing (in mM): 82.5 NaCl, 2.5 KCl, 1.0 MgCl2 , and 5.0 HEPES, pH 7.6, and 2–4 mg/ml collagenase (Worthington Biochemical Corp.). ..

    Isolation:

    Article Title: Plin2-deficiency reduces lipophagy and results in increased lipid accumulation in the heart
    Article Snippet: Bands were quantified with Image lab software (Bio-rad) and normalized to either GAPDH, HPRT or Na,K ATPase. .. Isolation of primary cardiomyocytes and cardiac fibroblasts Mice were anesthetized with isoflurane and the heart was rapidly excised, the aorta was cannulated and the heart was perfused retrogradely, initially with perfusion buffer alone (120 mM NaCl, 14.7 mM KCl, 0.6 mM KH2 PO4 , 0.6 mM Na2 HPO4, 1.2 mM MgSO4 , 10 mM Na-Hepes, 5.5 mM Glucose, 4.6 mM NaHCO3 , 30 mM Taurine, 10 mM BDM, pH 7) for 4 minutes, and the with the addition of Collagenase type 2 (230 u/mg dw) (Worthington) for 3 minutes and thereafter with digestion buffer together with 100 mM CaCl2 for 8 minutes on a Langendorff system (PanLab). .. After collagenase inhibition with fetal calf serum (FCS) (Thermofisher) the heart was torn apart and cardiomyocytes were separated from non-cardiomyocytes by centrifugation at 20 g for 3 minutes, the supernatant containing non-cardiomyocytes was used for fibroblast isolation.

    Mouse Assay:

    Article Title: Plin2-deficiency reduces lipophagy and results in increased lipid accumulation in the heart
    Article Snippet: Bands were quantified with Image lab software (Bio-rad) and normalized to either GAPDH, HPRT or Na,K ATPase. .. Isolation of primary cardiomyocytes and cardiac fibroblasts Mice were anesthetized with isoflurane and the heart was rapidly excised, the aorta was cannulated and the heart was perfused retrogradely, initially with perfusion buffer alone (120 mM NaCl, 14.7 mM KCl, 0.6 mM KH2 PO4 , 0.6 mM Na2 HPO4, 1.2 mM MgSO4 , 10 mM Na-Hepes, 5.5 mM Glucose, 4.6 mM NaHCO3 , 30 mM Taurine, 10 mM BDM, pH 7) for 4 minutes, and the with the addition of Collagenase type 2 (230 u/mg dw) (Worthington) for 3 minutes and thereafter with digestion buffer together with 100 mM CaCl2 for 8 minutes on a Langendorff system (PanLab). .. After collagenase inhibition with fetal calf serum (FCS) (Thermofisher) the heart was torn apart and cardiomyocytes were separated from non-cardiomyocytes by centrifugation at 20 g for 3 minutes, the supernatant containing non-cardiomyocytes was used for fibroblast isolation.

    ChIP-sequencing:

    Article Title: Chromatin remodeling during in vivo neural stem cells differentiating to neurons in early Drosophila embryos
    Article Snippet: To examine bead binding, DAPI-stained total and affinity-purified nuclei were counted on a hemacytometer, respectively. .. MNase-seq and ChIP-seq Affinity-purified nuclei were pelleted by centrifugation at 1000 × g for 5 min and suspended with 500 μ l of 37 °C preheated MNase (micrococcal nuclease) digestion buffer (10 mM (pH 7.5) Tris-HCl, 15 mM NaCl, 60 mM KCl, 2 mM CaCl2 , 0.15 mM spermine, 0.5 mM spermidine, 1 × PI) with 12 U MNase (Worthington Biochemical Corporation, Lakewood, NJ, USA) and incubated at 37 °C for 20 min. .. The reaction was terminated on ice by adding EDTA to a final concentration of 10 mM for 10 min.

    Affinity Purification:

    Article Title: Chromatin remodeling during in vivo neural stem cells differentiating to neurons in early Drosophila embryos
    Article Snippet: To examine bead binding, DAPI-stained total and affinity-purified nuclei were counted on a hemacytometer, respectively. .. MNase-seq and ChIP-seq Affinity-purified nuclei were pelleted by centrifugation at 1000 × g for 5 min and suspended with 500 μ l of 37 °C preheated MNase (micrococcal nuclease) digestion buffer (10 mM (pH 7.5) Tris-HCl, 15 mM NaCl, 60 mM KCl, 2 mM CaCl2 , 0.15 mM spermine, 0.5 mM spermidine, 1 × PI) with 12 U MNase (Worthington Biochemical Corporation, Lakewood, NJ, USA) and incubated at 37 °C for 20 min. .. The reaction was terminated on ice by adding EDTA to a final concentration of 10 mM for 10 min.

    Centrifugation:

    Article Title: Chromatin remodeling during in vivo neural stem cells differentiating to neurons in early Drosophila embryos
    Article Snippet: To examine bead binding, DAPI-stained total and affinity-purified nuclei were counted on a hemacytometer, respectively. .. MNase-seq and ChIP-seq Affinity-purified nuclei were pelleted by centrifugation at 1000 × g for 5 min and suspended with 500 μ l of 37 °C preheated MNase (micrococcal nuclease) digestion buffer (10 mM (pH 7.5) Tris-HCl, 15 mM NaCl, 60 mM KCl, 2 mM CaCl2 , 0.15 mM spermine, 0.5 mM spermidine, 1 × PI) with 12 U MNase (Worthington Biochemical Corporation, Lakewood, NJ, USA) and incubated at 37 °C for 20 min. .. The reaction was terminated on ice by adding EDTA to a final concentration of 10 mM for 10 min.

    Microscopy:

    Article Title: Eps15 Homology Domain-containing Protein 3 Regulates Cardiac T-type Ca2+ Channel Targeting and Function in the Atria *
    Article Snippet: .. Following enzymatic digestion, the heart was transferred to a dissecting microscope and atria were physically separated from the ventricles and placed into modified Tyrode solution (in m m : NaCl (137); KCl (5.4); MgCl2 (4.5); NaH2 PO4 (0.16), NaHCO3 (3); CaCl2 (10); HEPES-NaOH (20); glucose (10); taurine (1.3 mg/ml), pH 7.4) supplemented with collagenase II (Worthington, Type II) and protease (P-5147, Sigma). ..

    Modification:

    Article Title: Eps15 Homology Domain-containing Protein 3 Regulates Cardiac T-type Ca2+ Channel Targeting and Function in the Atria *
    Article Snippet: .. Following enzymatic digestion, the heart was transferred to a dissecting microscope and atria were physically separated from the ventricles and placed into modified Tyrode solution (in m m : NaCl (137); KCl (5.4); MgCl2 (4.5); NaH2 PO4 (0.16), NaHCO3 (3); CaCl2 (10); HEPES-NaOH (20); glucose (10); taurine (1.3 mg/ml), pH 7.4) supplemented with collagenase II (Worthington, Type II) and protease (P-5147, Sigma). ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    Worthington Biochemical hbss solution
    Hbss Solution, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hbss solution/product/Worthington Biochemical
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hbss solution - by Bioz Stars, 2021-03
    95/100 stars
      Buy from Supplier

    93
    Worthington Biochemical morpholinepropanesulfonic acid mops
    Morpholinepropanesulfonic Acid Mops, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/morpholinepropanesulfonic acid mops/product/Worthington Biochemical
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    morpholinepropanesulfonic acid mops - by Bioz Stars, 2021-03
    93/100 stars
      Buy from Supplier

    Image Search Results