cfpure cell free dna purification kit  (AMS Biotechnology)

Journal: Micromachines

Article Title: Salivary Exosome and Cell-Free DNA for Cancer Detection

doi: 10.3390/mi9070340

Figure Lengend Snippet: Applicable technologies for isolation of circulating biomarkers in saliva.

Article Snippet: The cfPure™ cell-free DNA purification kit (Amsbio) uses silica-coated paramagnetic particles with a specific buffer optimized for efficient recovery of 100–500 bp DNA fragments.

Techniques: Isolation, Size-exclusion Chromatography, Filtration, Electrophoresis, Purification, DNA Extraction, Column Chromatography, Ethanol Precipitation, Digital PCR, Mutagenesis, DNA Purification, Amplification, Concentration Assay, Real-time Polymerase Chain Reaction

Journal: Micromachines

Article Title: Salivary Exosome and Cell-Free DNA for Cancer Detection

doi: 10.3390/mi9070340

Figure Lengend Snippet: Microfluidic devices for exosome and cell-free DNA research. ( a ) Multistage exosome microfluidic for immunomagnetic isolation of exosome, exosome lysis, protein capture, and intravesicular protein analysis; ( b ) PPM membrane based microfluidic system for exosome filtration; ( c ) Droplet-based microfluidic device for detecting mutated DNA in a quantitative manner. Reproduced from ref. [ , , ] with permission from 2014 Royal Society of Chemistry, 2012 Royal Society of Chemistry, and 2011 Royal Society of Chemistry.

Article Snippet: The cfPure™ cell-free DNA purification kit (Amsbio) uses silica-coated paramagnetic particles with a specific buffer optimized for efficient recovery of 100–500 bp DNA fragments.

Techniques: Isolation, Lysis, Filtration

Journal: Nature biotechnology

Article Title: Detection of low-frequency DNA variants by targeted sequencing of the Watson and Crick strands

doi: 10.1038/s41587-021-00900-z

Figure Lengend Snippet: Analysis of 33 ng of plasma cell-free DNA from healthy individuals admixed with cell-free plasma DNA from an individual with cancer. Mixtures were created to generate a high frequency (~0.5–1%) of mutation (blue bars), low frequency (~0.01–0.1%) of mutation (orange bars) or no mutation (gray bars). The admixed TP53 p.R342X sample was assayed with SafeSeqs (a) and SaferSeqS (b). Similarly, the admixed TP53 p.L264fs sample was assayed with SafeSeqs (c) and SaferSeqS (d), and the admixed TP53 p.P190L sample was assayed with SafeSeqs (e) and SaferSeqS (f). Mutation numbers represent each of the 153 distinct mutations observed with SafeSeqS defined in Supplementary Table 2.

Article Snippet: DNA was purified from plasma using a cfPure MAX Cell-Free DNA Extraction Kit (BioChain, K5011625MA), as specified by the manufacturer’s instructions.

Techniques: Mutagenesis