k252b  (Alomone Labs)


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    Alomone Labs k252b
    K252b, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k252b/product/Alomone Labs
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    k252b - by Bioz Stars, 2023-01
    85/100 stars

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    k252b  (Alomone Labs)


    Bioz Verified Symbol Alomone Labs is a verified supplier
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  • 86

    Structured Review

    Alomone Labs k252b
    K252b, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k252b/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    k252b - by Bioz Stars, 2023-01
    86/100 stars

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    k252b  (Alomone Labs)


    Bioz Verified Symbol Alomone Labs is a verified supplier
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  • 85

    Structured Review

    Alomone Labs k252b
    K252b, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k252b/product/Alomone Labs
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    k252b - by Bioz Stars, 2023-01
    85/100 stars

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    k252b  (Alomone Labs)


    Bioz Verified Symbol Alomone Labs is a verified supplier
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    Alomone Labs k252b
    A, peak amplitude of responses to GABA (20 μm) plotted as a function of time before, during and after exposure to 100 ng ml−1 BDNF. The continuous line denotes time and duration of BDNF application. GABA responses were monitored in normal bath solution without exposure to BDNF during recording (□), in normal bath solution with a 1 min exposure to BDNF (▪), and with added K252a (200 nm; ○), <t>K252b</t> (200 nm; •) and TrkB-IgG (5 μg ml−1; ▵). Each point represents data averaged from 8–10 cells. B, bar graph summarizing mean peak amplitude of GABA responses as a function of the conditions in A.
    K252b, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k252b/product/Alomone Labs
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    k252b - by Bioz Stars, 2023-01
    85/100 stars

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    1) Product Images from "Brain-derived neurotrophic factor attenuates mouse cerebellar granule cell GABA A receptor-mediated responses via postsynaptic mechanisms"

    Article Title: Brain-derived neurotrophic factor attenuates mouse cerebellar granule cell GABA A receptor-mediated responses via postsynaptic mechanisms

    Journal:

    doi: 10.1113/jphysiol.2002.037846

    A, peak amplitude of responses to GABA (20 μm) plotted as a function of time before, during and after exposure to 100 ng ml−1 BDNF. The continuous line denotes time and duration of BDNF application. GABA responses were monitored in normal bath solution without exposure to BDNF during recording (□), in normal bath solution with a 1 min exposure to BDNF (▪), and with added K252a (200 nm; ○), K252b (200 nm; •) and TrkB-IgG (5 μg ml−1; ▵). Each point represents data averaged from 8–10 cells. B, bar graph summarizing mean peak amplitude of GABA responses as a function of the conditions in A.
    Figure Legend Snippet: A, peak amplitude of responses to GABA (20 μm) plotted as a function of time before, during and after exposure to 100 ng ml−1 BDNF. The continuous line denotes time and duration of BDNF application. GABA responses were monitored in normal bath solution without exposure to BDNF during recording (□), in normal bath solution with a 1 min exposure to BDNF (▪), and with added K252a (200 nm; ○), K252b (200 nm; •) and TrkB-IgG (5 μg ml−1; ▵). Each point represents data averaged from 8–10 cells. B, bar graph summarizing mean peak amplitude of GABA responses as a function of the conditions in A.

    Techniques Used:

    Confocal images of cultured cerebellar granule cells before (A) and following a 60 min exposure to BDNF (100 ng ml−1) (B, C and D). The cultures were fixed and co-stained with antibodies against GABAA receptor β2/3 subunits (green fluorescence) and TrkB receptors (red fluorescence). GABAA receptor β2/3 subunit immunoreactivity, illustrated in A, is markedly reduced following exposure to BDNF (B). C and D, addition of K252a (200 nm) prevented the BDNF-induced reduction of GABAA receptor β2/3 subunit immunoreactivity (C), which persisted in the presence of K252b (200 nm; D). Scale bar (applies to all panels), 50 μm.
    Figure Legend Snippet: Confocal images of cultured cerebellar granule cells before (A) and following a 60 min exposure to BDNF (100 ng ml−1) (B, C and D). The cultures were fixed and co-stained with antibodies against GABAA receptor β2/3 subunits (green fluorescence) and TrkB receptors (red fluorescence). GABAA receptor β2/3 subunit immunoreactivity, illustrated in A, is markedly reduced following exposure to BDNF (B). C and D, addition of K252a (200 nm) prevented the BDNF-induced reduction of GABAA receptor β2/3 subunit immunoreactivity (C), which persisted in the presence of K252b (200 nm; D). Scale bar (applies to all panels), 50 μm.

    Techniques Used: Cell Culture, Staining, Fluorescence

    k252b  (Alomone Labs)


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    Alomone Labs k252b
    A, peak amplitude of responses to GABA (20 μm) plotted as a function of time before, during and after exposure to 100 ng ml−1 BDNF. The continuous line denotes time and duration of BDNF application. GABA responses were monitored in normal bath solution without exposure to BDNF during recording (□), in normal bath solution with a 1 min exposure to BDNF (▪), and with added K252a (200 nm; ○), <t>K252b</t> (200 nm; •) and TrkB-IgG (5 μg ml−1; ▵). Each point represents data averaged from 8–10 cells. B, bar graph summarizing mean peak amplitude of GABA responses as a function of the conditions in A.
    K252b, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k252b/product/Alomone Labs
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    k252b - by Bioz Stars, 2023-01
    85/100 stars

    Images

    1) Product Images from "Brain-derived neurotrophic factor attenuates mouse cerebellar granule cell GABA A receptor-mediated responses via postsynaptic mechanisms"

    Article Title: Brain-derived neurotrophic factor attenuates mouse cerebellar granule cell GABA A receptor-mediated responses via postsynaptic mechanisms

    Journal:

    doi: 10.1113/jphysiol.2002.037846

    A, peak amplitude of responses to GABA (20 μm) plotted as a function of time before, during and after exposure to 100 ng ml−1 BDNF. The continuous line denotes time and duration of BDNF application. GABA responses were monitored in normal bath solution without exposure to BDNF during recording (□), in normal bath solution with a 1 min exposure to BDNF (▪), and with added K252a (200 nm; ○), K252b (200 nm; •) and TrkB-IgG (5 μg ml−1; ▵). Each point represents data averaged from 8–10 cells. B, bar graph summarizing mean peak amplitude of GABA responses as a function of the conditions in A.
    Figure Legend Snippet: A, peak amplitude of responses to GABA (20 μm) plotted as a function of time before, during and after exposure to 100 ng ml−1 BDNF. The continuous line denotes time and duration of BDNF application. GABA responses were monitored in normal bath solution without exposure to BDNF during recording (□), in normal bath solution with a 1 min exposure to BDNF (▪), and with added K252a (200 nm; ○), K252b (200 nm; •) and TrkB-IgG (5 μg ml−1; ▵). Each point represents data averaged from 8–10 cells. B, bar graph summarizing mean peak amplitude of GABA responses as a function of the conditions in A.

    Techniques Used:

    Confocal images of cultured cerebellar granule cells before (A) and following a 60 min exposure to BDNF (100 ng ml−1) (B, C and D). The cultures were fixed and co-stained with antibodies against GABAA receptor β2/3 subunits (green fluorescence) and TrkB receptors (red fluorescence). GABAA receptor β2/3 subunit immunoreactivity, illustrated in A, is markedly reduced following exposure to BDNF (B). C and D, addition of K252a (200 nm) prevented the BDNF-induced reduction of GABAA receptor β2/3 subunit immunoreactivity (C), which persisted in the presence of K252b (200 nm; D). Scale bar (applies to all panels), 50 μm.
    Figure Legend Snippet: Confocal images of cultured cerebellar granule cells before (A) and following a 60 min exposure to BDNF (100 ng ml−1) (B, C and D). The cultures were fixed and co-stained with antibodies against GABAA receptor β2/3 subunits (green fluorescence) and TrkB receptors (red fluorescence). GABAA receptor β2/3 subunit immunoreactivity, illustrated in A, is markedly reduced following exposure to BDNF (B). C and D, addition of K252a (200 nm) prevented the BDNF-induced reduction of GABAA receptor β2/3 subunit immunoreactivity (C), which persisted in the presence of K252b (200 nm; D). Scale bar (applies to all panels), 50 μm.

    Techniques Used: Cell Culture, Staining, Fluorescence

    k252b  (Alomone Labs)


    Bioz Verified Symbol Alomone Labs is a verified supplier
    Bioz Manufacturer Symbol Alomone Labs manufactures this product  
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  • 85

    Structured Review

    Alomone Labs k252b
    K252b, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k252b/product/Alomone Labs
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    k252b - by Bioz Stars, 2023-01
    85/100 stars

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    Alomone Labs k252b
    K252b, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k252b/product/Alomone Labs
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    k252b - by Bioz Stars, 2023-01
    85/100 stars
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