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Electron microscopical analysis of caveolin-1 in cultured human ARPE-19 cells. (A) Micrograph of an ARPE-19 cell depicting apical microvilli (AM) and basal infoldings (BI). The nucleus (N) can also be seen (7700×). Panel A serves as a comparison for panels B–E, which are higher magnifications of the apical and basal plasma membranes of a RPE cell. (B) Higher magnification of apical microvilli labelled with an antibody against caveolin-1. No gold particles were observed (60,000×). (C) Higher magnification of the basal infoldings of RPE labelled with an antibody against caveolin-1. Arrows point to gold particles (35,500×). (D) Higher magnification of apical microvilli labelled with antibody against the Na + ,K + <t>-ATPase.</t> Gold particles were abundant (46,000×). (E) Higher magnification of the basolateral infoldings of A RPE cells labelled with an antibody against folate receptor <t>α</t> . Gold particles were present across the membrane (46,000×).
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1) Product Images from "A comparison of caveolae and caveolin-1 to folate receptor α in retina and retinal pigment epithelium"

Article Title: A comparison of caveolae and caveolin-1 to folate receptor α in retina and retinal pigment epithelium

Journal: The Histochemical journal

doi:

Electron microscopical analysis of caveolin-1 in cultured human ARPE-19 cells. (A) Micrograph of an ARPE-19 cell depicting apical microvilli (AM) and basal infoldings (BI). The nucleus (N) can also be seen (7700×). Panel A serves as a comparison for panels B–E, which are higher magnifications of the apical and basal plasma membranes of a RPE cell. (B) Higher magnification of apical microvilli labelled with an antibody against caveolin-1. No gold particles were observed (60,000×). (C) Higher magnification of the basal infoldings of RPE labelled with an antibody against caveolin-1. Arrows point to gold particles (35,500×). (D) Higher magnification of apical microvilli labelled with antibody against the Na + ,K + -ATPase. Gold particles were abundant (46,000×). (E) Higher magnification of the basolateral infoldings of A RPE cells labelled with an antibody against folate receptor α . Gold particles were present across the membrane (46,000×).
Figure Legend Snippet: Electron microscopical analysis of caveolin-1 in cultured human ARPE-19 cells. (A) Micrograph of an ARPE-19 cell depicting apical microvilli (AM) and basal infoldings (BI). The nucleus (N) can also be seen (7700×). Panel A serves as a comparison for panels B–E, which are higher magnifications of the apical and basal plasma membranes of a RPE cell. (B) Higher magnification of apical microvilli labelled with an antibody against caveolin-1. No gold particles were observed (60,000×). (C) Higher magnification of the basal infoldings of RPE labelled with an antibody against caveolin-1. Arrows point to gold particles (35,500×). (D) Higher magnification of apical microvilli labelled with antibody against the Na + ,K + -ATPase. Gold particles were abundant (46,000×). (E) Higher magnification of the basolateral infoldings of A RPE cells labelled with an antibody against folate receptor α . Gold particles were present across the membrane (46,000×).

Techniques Used: Cell Culture

Laser scanning confocal microscopic localization of caveolin-1 and folate receptor α in intact mouse RPE. (A) High magnification of mouse retina incubated with an antibody against caveolin-1. The apical and basal plasma membranes of the RPE are represented by the top (a) and bottom (b) two-headed arrows, respectively. Caveolin-1 is present on the apical (arrows) and basal (arrowheads) plasmalemmal surfaces of the RPE cell. (B) High magnification of mouse retina incubated with an antibody against the folate receptor α . Note the intense bands of fluorescence on the basolateral membrane of RPE, but no immunoreactivity in the apical region. (C) High magnification of mouse retina incubated with an antibody against the Na + ,K + -ATPase. As expected, an intense band of fluorescent labelling was observed on the apical surface of the RPE monolayer. (D) Haematoxylin and eosin-stained section of mouse retina shown for comparison (630×). Abbreviations: IS, inner segments; OS, outer segments; RPE, retinal pigment epithelium.
Figure Legend Snippet: Laser scanning confocal microscopic localization of caveolin-1 and folate receptor α in intact mouse RPE. (A) High magnification of mouse retina incubated with an antibody against caveolin-1. The apical and basal plasma membranes of the RPE are represented by the top (a) and bottom (b) two-headed arrows, respectively. Caveolin-1 is present on the apical (arrows) and basal (arrowheads) plasmalemmal surfaces of the RPE cell. (B) High magnification of mouse retina incubated with an antibody against the folate receptor α . Note the intense bands of fluorescence on the basolateral membrane of RPE, but no immunoreactivity in the apical region. (C) High magnification of mouse retina incubated with an antibody against the Na + ,K + -ATPase. As expected, an intense band of fluorescent labelling was observed on the apical surface of the RPE monolayer. (D) Haematoxylin and eosin-stained section of mouse retina shown for comparison (630×). Abbreviations: IS, inner segments; OS, outer segments; RPE, retinal pigment epithelium.

Techniques Used: Incubation, Fluorescence, Staining

Electron microscopical immunohistochemical analysis of caveolin-1 in mammalian retina. (A) Mouse RPE depicting apical microvilli (AM) and basal infoldings (BI). The nucleus (N) and mitochondria (M) are also visible (7700×). Panel A serves as a comparison for panels B–E, which are higher magnifications of the apical and basal plasma membranes of a RPE cell. (B) Higher magnification of apical microvilli labelled with an antibody against caveolin-1. Arrows point to gold particles indicative of positive labelling (27,500×). (C) Higher magnification of the basal infoldings of RPE labelled with an antibody against caveolin-1. Arrows point to gold particles (35,500×). (D) Higher magnification of apical microvilli labelled with antibody against the Na + ,K + -ATPase (60,000×). Gold particles were abundant. (E) Higher magnification of the basolateral infoldings of a RPE cell labelled with an antibody against folate receptor α (60,000×). Gold particles were present across the membrane.
Figure Legend Snippet: Electron microscopical immunohistochemical analysis of caveolin-1 in mammalian retina. (A) Mouse RPE depicting apical microvilli (AM) and basal infoldings (BI). The nucleus (N) and mitochondria (M) are also visible (7700×). Panel A serves as a comparison for panels B–E, which are higher magnifications of the apical and basal plasma membranes of a RPE cell. (B) Higher magnification of apical microvilli labelled with an antibody against caveolin-1. Arrows point to gold particles indicative of positive labelling (27,500×). (C) Higher magnification of the basal infoldings of RPE labelled with an antibody against caveolin-1. Arrows point to gold particles (35,500×). (D) Higher magnification of apical microvilli labelled with antibody against the Na + ,K + -ATPase (60,000×). Gold particles were abundant. (E) Higher magnification of the basolateral infoldings of a RPE cell labelled with an antibody against folate receptor α (60,000×). Gold particles were present across the membrane.

Techniques Used: Immunohistochemistry

2) Product Images from "A comparison of caveolae and caveolin-1 to folate receptor α in retina and retinal pigment epithelium"

Article Title: A comparison of caveolae and caveolin-1 to folate receptor α in retina and retinal pigment epithelium

Journal: The Histochemical journal

doi:

Electron microscopical analysis of caveolin-1 in cultured human ARPE-19 cells. (A) Micrograph of an ARPE-19 cell depicting apical microvilli (AM) and basal infoldings (BI). The nucleus (N) can also be seen (7700×). Panel A serves as a comparison for panels B–E, which are higher magnifications of the apical and basal plasma membranes of a RPE cell. (B) Higher magnification of apical microvilli labelled with an antibody against caveolin-1. No gold particles were observed (60,000×). (C) Higher magnification of the basal infoldings of RPE labelled with an antibody against caveolin-1. Arrows point to gold particles (35,500×). (D) Higher magnification of apical microvilli labelled with antibody against the Na + ,K + -ATPase. Gold particles were abundant (46,000×). (E) Higher magnification of the basolateral infoldings of A RPE cells labelled with an antibody against folate receptor α . Gold particles were present across the membrane (46,000×).
Figure Legend Snippet: Electron microscopical analysis of caveolin-1 in cultured human ARPE-19 cells. (A) Micrograph of an ARPE-19 cell depicting apical microvilli (AM) and basal infoldings (BI). The nucleus (N) can also be seen (7700×). Panel A serves as a comparison for panels B–E, which are higher magnifications of the apical and basal plasma membranes of a RPE cell. (B) Higher magnification of apical microvilli labelled with an antibody against caveolin-1. No gold particles were observed (60,000×). (C) Higher magnification of the basal infoldings of RPE labelled with an antibody against caveolin-1. Arrows point to gold particles (35,500×). (D) Higher magnification of apical microvilli labelled with antibody against the Na + ,K + -ATPase. Gold particles were abundant (46,000×). (E) Higher magnification of the basolateral infoldings of A RPE cells labelled with an antibody against folate receptor α . Gold particles were present across the membrane (46,000×).

Techniques Used: Cell Culture

Electron microscopical immunohistochemical analysis of caveolin-1 in mammalian retina. (A) Mouse RPE depicting apical microvilli (AM) and basal infoldings (BI). The nucleus (N) and mitochondria (M) are also visible (7700×). Panel A serves as a comparison for panels B–E, which are higher magnifications of the apical and basal plasma membranes of a RPE cell. (B) Higher magnification of apical microvilli labelled with an antibody against caveolin-1. Arrows point to gold particles indicative of positive labelling (27,500×). (C) Higher magnification of the basal infoldings of RPE labelled with an antibody against caveolin-1. Arrows point to gold particles (35,500×). (D) Higher magnification of apical microvilli labelled with antibody against the Na + ,K + -ATPase (60,000×). Gold particles were abundant. (E) Higher magnification of the basolateral infoldings of a RPE cell labelled with an antibody against folate receptor α (60,000×). Gold particles were present across the membrane.
Figure Legend Snippet: Electron microscopical immunohistochemical analysis of caveolin-1 in mammalian retina. (A) Mouse RPE depicting apical microvilli (AM) and basal infoldings (BI). The nucleus (N) and mitochondria (M) are also visible (7700×). Panel A serves as a comparison for panels B–E, which are higher magnifications of the apical and basal plasma membranes of a RPE cell. (B) Higher magnification of apical microvilli labelled with an antibody against caveolin-1. Arrows point to gold particles indicative of positive labelling (27,500×). (C) Higher magnification of the basal infoldings of RPE labelled with an antibody against caveolin-1. Arrows point to gold particles (35,500×). (D) Higher magnification of apical microvilli labelled with antibody against the Na + ,K + -ATPase (60,000×). Gold particles were abundant. (E) Higher magnification of the basolateral infoldings of a RPE cell labelled with an antibody against folate receptor α (60,000×). Gold particles were present across the membrane.

Techniques Used: Immunohistochemistry

3) Product Images from "A comparison of caveolae and caveolin-1 to folate receptor α in retina and retinal pigment epithelium"

Article Title: A comparison of caveolae and caveolin-1 to folate receptor α in retina and retinal pigment epithelium

Journal: The Histochemical journal

doi:

Electron microscopical analysis of caveolin-1 in cultured human ARPE-19 cells. (A) Micrograph of an ARPE-19 cell depicting apical microvilli (AM) and basal infoldings (BI). The nucleus (N) can also be seen (7700×). Panel A serves as a comparison for panels B–E, which are higher magnifications of the apical and basal plasma membranes of a RPE cell. (B) Higher magnification of apical microvilli labelled with an antibody against caveolin-1. No gold particles were observed (60,000×). (C) Higher magnification of the basal infoldings of RPE labelled with an antibody against caveolin-1. Arrows point to gold particles (35,500×). (D) Higher magnification of apical microvilli labelled with antibody against the Na + ,K + -ATPase. Gold particles were abundant (46,000×). (E) Higher magnification of the basolateral infoldings of A RPE cells labelled with an antibody against folate receptor α . Gold particles were present across the membrane (46,000×).
Figure Legend Snippet: Electron microscopical analysis of caveolin-1 in cultured human ARPE-19 cells. (A) Micrograph of an ARPE-19 cell depicting apical microvilli (AM) and basal infoldings (BI). The nucleus (N) can also be seen (7700×). Panel A serves as a comparison for panels B–E, which are higher magnifications of the apical and basal plasma membranes of a RPE cell. (B) Higher magnification of apical microvilli labelled with an antibody against caveolin-1. No gold particles were observed (60,000×). (C) Higher magnification of the basal infoldings of RPE labelled with an antibody against caveolin-1. Arrows point to gold particles (35,500×). (D) Higher magnification of apical microvilli labelled with antibody against the Na + ,K + -ATPase. Gold particles were abundant (46,000×). (E) Higher magnification of the basolateral infoldings of A RPE cells labelled with an antibody against folate receptor α . Gold particles were present across the membrane (46,000×).

Techniques Used: Cell Culture

Laser scanning confocal microscopic localization of caveolin-1 and folate receptor α in intact mouse RPE. (A) High magnification of mouse retina incubated with an antibody against caveolin-1. The apical and basal plasma membranes of the RPE are represented by the top (a) and bottom (b) two-headed arrows, respectively. Caveolin-1 is present on the apical (arrows) and basal (arrowheads) plasmalemmal surfaces of the RPE cell. (B) High magnification of mouse retina incubated with an antibody against the folate receptor α . Note the intense bands of fluorescence on the basolateral membrane of RPE, but no immunoreactivity in the apical region. (C) High magnification of mouse retina incubated with an antibody against the Na + ,K + -ATPase. As expected, an intense band of fluorescent labelling was observed on the apical surface of the RPE monolayer. (D) Haematoxylin and eosin-stained section of mouse retina shown for comparison (630×). Abbreviations: IS, inner segments; OS, outer segments; RPE, retinal pigment epithelium.
Figure Legend Snippet: Laser scanning confocal microscopic localization of caveolin-1 and folate receptor α in intact mouse RPE. (A) High magnification of mouse retina incubated with an antibody against caveolin-1. The apical and basal plasma membranes of the RPE are represented by the top (a) and bottom (b) two-headed arrows, respectively. Caveolin-1 is present on the apical (arrows) and basal (arrowheads) plasmalemmal surfaces of the RPE cell. (B) High magnification of mouse retina incubated with an antibody against the folate receptor α . Note the intense bands of fluorescence on the basolateral membrane of RPE, but no immunoreactivity in the apical region. (C) High magnification of mouse retina incubated with an antibody against the Na + ,K + -ATPase. As expected, an intense band of fluorescent labelling was observed on the apical surface of the RPE monolayer. (D) Haematoxylin and eosin-stained section of mouse retina shown for comparison (630×). Abbreviations: IS, inner segments; OS, outer segments; RPE, retinal pigment epithelium.

Techniques Used: Incubation, Fluorescence, Staining

Electron microscopical immunohistochemical analysis of caveolin-1 in mammalian retina. (A) Mouse RPE depicting apical microvilli (AM) and basal infoldings (BI). The nucleus (N) and mitochondria (M) are also visible (7700×). Panel A serves as a comparison for panels B–E, which are higher magnifications of the apical and basal plasma membranes of a RPE cell. (B) Higher magnification of apical microvilli labelled with an antibody against caveolin-1. Arrows point to gold particles indicative of positive labelling (27,500×). (C) Higher magnification of the basal infoldings of RPE labelled with an antibody against caveolin-1. Arrows point to gold particles (35,500×). (D) Higher magnification of apical microvilli labelled with antibody against the Na + ,K + -ATPase (60,000×). Gold particles were abundant. (E) Higher magnification of the basolateral infoldings of a RPE cell labelled with an antibody against folate receptor α (60,000×). Gold particles were present across the membrane.
Figure Legend Snippet: Electron microscopical immunohistochemical analysis of caveolin-1 in mammalian retina. (A) Mouse RPE depicting apical microvilli (AM) and basal infoldings (BI). The nucleus (N) and mitochondria (M) are also visible (7700×). Panel A serves as a comparison for panels B–E, which are higher magnifications of the apical and basal plasma membranes of a RPE cell. (B) Higher magnification of apical microvilli labelled with an antibody against caveolin-1. Arrows point to gold particles indicative of positive labelling (27,500×). (C) Higher magnification of the basal infoldings of RPE labelled with an antibody against caveolin-1. Arrows point to gold particles (35,500×). (D) Higher magnification of apical microvilli labelled with antibody against the Na + ,K + -ATPase (60,000×). Gold particles were abundant. (E) Higher magnification of the basolateral infoldings of a RPE cell labelled with an antibody against folate receptor α (60,000×). Gold particles were present across the membrane.

Techniques Used: Immunohistochemistry

4) Product Images from "A comparison of caveolae and caveolin-1 to folate receptor α in retina and retinal pigment epithelium"

Article Title: A comparison of caveolae and caveolin-1 to folate receptor α in retina and retinal pigment epithelium

Journal: The Histochemical journal

doi:

Electron microscopical analysis of caveolin-1 in cultured human ARPE-19 cells. (A) Micrograph of an ARPE-19 cell depicting apical microvilli (AM) and basal infoldings (BI). The nucleus (N) can also be seen (7700×). Panel A serves as a comparison for panels B–E, which are higher magnifications of the apical and basal plasma membranes of a RPE cell. (B) Higher magnification of apical microvilli labelled with an antibody against caveolin-1. No gold particles were observed (60,000×). (C) Higher magnification of the basal infoldings of RPE labelled with an antibody against caveolin-1. Arrows point to gold particles (35,500×). (D) Higher magnification of apical microvilli labelled with antibody against the Na + ,K + -ATPase. Gold particles were abundant (46,000×). (E) Higher magnification of the basolateral infoldings of A RPE cells labelled with an antibody against folate receptor α . Gold particles were present across the membrane (46,000×).
Figure Legend Snippet: Electron microscopical analysis of caveolin-1 in cultured human ARPE-19 cells. (A) Micrograph of an ARPE-19 cell depicting apical microvilli (AM) and basal infoldings (BI). The nucleus (N) can also be seen (7700×). Panel A serves as a comparison for panels B–E, which are higher magnifications of the apical and basal plasma membranes of a RPE cell. (B) Higher magnification of apical microvilli labelled with an antibody against caveolin-1. No gold particles were observed (60,000×). (C) Higher magnification of the basal infoldings of RPE labelled with an antibody against caveolin-1. Arrows point to gold particles (35,500×). (D) Higher magnification of apical microvilli labelled with antibody against the Na + ,K + -ATPase. Gold particles were abundant (46,000×). (E) Higher magnification of the basolateral infoldings of A RPE cells labelled with an antibody against folate receptor α . Gold particles were present across the membrane (46,000×).

Techniques Used: Cell Culture

Laser scanning confocal microscopic localization of caveolin-1 and folate receptor α in intact mouse RPE. (A) High magnification of mouse retina incubated with an antibody against caveolin-1. The apical and basal plasma membranes of the RPE are represented by the top (a) and bottom (b) two-headed arrows, respectively. Caveolin-1 is present on the apical (arrows) and basal (arrowheads) plasmalemmal surfaces of the RPE cell. (B) High magnification of mouse retina incubated with an antibody against the folate receptor α . Note the intense bands of fluorescence on the basolateral membrane of RPE, but no immunoreactivity in the apical region. (C) High magnification of mouse retina incubated with an antibody against the Na + ,K + -ATPase. As expected, an intense band of fluorescent labelling was observed on the apical surface of the RPE monolayer. (D) Haematoxylin and eosin-stained section of mouse retina shown for comparison (630×). Abbreviations: IS, inner segments; OS, outer segments; RPE, retinal pigment epithelium.
Figure Legend Snippet: Laser scanning confocal microscopic localization of caveolin-1 and folate receptor α in intact mouse RPE. (A) High magnification of mouse retina incubated with an antibody against caveolin-1. The apical and basal plasma membranes of the RPE are represented by the top (a) and bottom (b) two-headed arrows, respectively. Caveolin-1 is present on the apical (arrows) and basal (arrowheads) plasmalemmal surfaces of the RPE cell. (B) High magnification of mouse retina incubated with an antibody against the folate receptor α . Note the intense bands of fluorescence on the basolateral membrane of RPE, but no immunoreactivity in the apical region. (C) High magnification of mouse retina incubated with an antibody against the Na + ,K + -ATPase. As expected, an intense band of fluorescent labelling was observed on the apical surface of the RPE monolayer. (D) Haematoxylin and eosin-stained section of mouse retina shown for comparison (630×). Abbreviations: IS, inner segments; OS, outer segments; RPE, retinal pigment epithelium.

Techniques Used: Incubation, Fluorescence, Staining

Electron microscopical immunohistochemical analysis of caveolin-1 in mammalian retina. (A) Mouse RPE depicting apical microvilli (AM) and basal infoldings (BI). The nucleus (N) and mitochondria (M) are also visible (7700×). Panel A serves as a comparison for panels B–E, which are higher magnifications of the apical and basal plasma membranes of a RPE cell. (B) Higher magnification of apical microvilli labelled with an antibody against caveolin-1. Arrows point to gold particles indicative of positive labelling (27,500×). (C) Higher magnification of the basal infoldings of RPE labelled with an antibody against caveolin-1. Arrows point to gold particles (35,500×). (D) Higher magnification of apical microvilli labelled with antibody against the Na + ,K + -ATPase (60,000×). Gold particles were abundant. (E) Higher magnification of the basolateral infoldings of a RPE cell labelled with an antibody against folate receptor α (60,000×). Gold particles were present across the membrane.
Figure Legend Snippet: Electron microscopical immunohistochemical analysis of caveolin-1 in mammalian retina. (A) Mouse RPE depicting apical microvilli (AM) and basal infoldings (BI). The nucleus (N) and mitochondria (M) are also visible (7700×). Panel A serves as a comparison for panels B–E, which are higher magnifications of the apical and basal plasma membranes of a RPE cell. (B) Higher magnification of apical microvilli labelled with an antibody against caveolin-1. Arrows point to gold particles indicative of positive labelling (27,500×). (C) Higher magnification of the basal infoldings of RPE labelled with an antibody against caveolin-1. Arrows point to gold particles (35,500×). (D) Higher magnification of apical microvilli labelled with antibody against the Na + ,K + -ATPase (60,000×). Gold particles were abundant. (E) Higher magnification of the basolateral infoldings of a RPE cell labelled with an antibody against folate receptor α (60,000×). Gold particles were present across the membrane.

Techniques Used: Immunohistochemistry

5) Product Images from "A comparison of caveolae and caveolin-1 to folate receptor α in retina and retinal pigment epithelium"

Article Title: A comparison of caveolae and caveolin-1 to folate receptor α in retina and retinal pigment epithelium

Journal: The Histochemical journal

doi:

Electron microscopical analysis of caveolin-1 in cultured human ARPE-19 cells. (A) Micrograph of an ARPE-19 cell depicting apical microvilli (AM) and basal infoldings (BI). The nucleus (N) can also be seen (7700×). Panel A serves as a comparison for panels B–E, which are higher magnifications of the apical and basal plasma membranes of a RPE cell. (B) Higher magnification of apical microvilli labelled with an antibody against caveolin-1. No gold particles were observed (60,000×). (C) Higher magnification of the basal infoldings of RPE labelled with an antibody against caveolin-1. Arrows point to gold particles (35,500×). (D) Higher magnification of apical microvilli labelled with antibody against the Na + ,K + -ATPase. Gold particles were abundant (46,000×). (E) Higher magnification of the basolateral infoldings of A RPE cells labelled with an antibody against folate receptor α . Gold particles were present across the membrane (46,000×).
Figure Legend Snippet: Electron microscopical analysis of caveolin-1 in cultured human ARPE-19 cells. (A) Micrograph of an ARPE-19 cell depicting apical microvilli (AM) and basal infoldings (BI). The nucleus (N) can also be seen (7700×). Panel A serves as a comparison for panels B–E, which are higher magnifications of the apical and basal plasma membranes of a RPE cell. (B) Higher magnification of apical microvilli labelled with an antibody against caveolin-1. No gold particles were observed (60,000×). (C) Higher magnification of the basal infoldings of RPE labelled with an antibody against caveolin-1. Arrows point to gold particles (35,500×). (D) Higher magnification of apical microvilli labelled with antibody against the Na + ,K + -ATPase. Gold particles were abundant (46,000×). (E) Higher magnification of the basolateral infoldings of A RPE cells labelled with an antibody against folate receptor α . Gold particles were present across the membrane (46,000×).

Techniques Used: Cell Culture

Electron microscopical immunohistochemical analysis of caveolin-1 in mammalian retina. (A) Mouse RPE depicting apical microvilli (AM) and basal infoldings (BI). The nucleus (N) and mitochondria (M) are also visible (7700×). Panel A serves as a comparison for panels B–E, which are higher magnifications of the apical and basal plasma membranes of a RPE cell. (B) Higher magnification of apical microvilli labelled with an antibody against caveolin-1. Arrows point to gold particles indicative of positive labelling (27,500×). (C) Higher magnification of the basal infoldings of RPE labelled with an antibody against caveolin-1. Arrows point to gold particles (35,500×). (D) Higher magnification of apical microvilli labelled with antibody against the Na + ,K + -ATPase (60,000×). Gold particles were abundant. (E) Higher magnification of the basolateral infoldings of a RPE cell labelled with an antibody against folate receptor α (60,000×). Gold particles were present across the membrane.
Figure Legend Snippet: Electron microscopical immunohistochemical analysis of caveolin-1 in mammalian retina. (A) Mouse RPE depicting apical microvilli (AM) and basal infoldings (BI). The nucleus (N) and mitochondria (M) are also visible (7700×). Panel A serves as a comparison for panels B–E, which are higher magnifications of the apical and basal plasma membranes of a RPE cell. (B) Higher magnification of apical microvilli labelled with an antibody against caveolin-1. Arrows point to gold particles indicative of positive labelling (27,500×). (C) Higher magnification of the basal infoldings of RPE labelled with an antibody against caveolin-1. Arrows point to gold particles (35,500×). (D) Higher magnification of apical microvilli labelled with antibody against the Na + ,K + -ATPase (60,000×). Gold particles were abundant. (E) Higher magnification of the basolateral infoldings of a RPE cell labelled with an antibody against folate receptor α (60,000×). Gold particles were present across the membrane.

Techniques Used: Immunohistochemistry

6) Product Images from "A comparison of caveolae and caveolin-1 to folate receptor α in retina and retinal pigment epithelium"

Article Title: A comparison of caveolae and caveolin-1 to folate receptor α in retina and retinal pigment epithelium

Journal: The Histochemical journal

doi:

Electron microscopical analysis of caveolin-1 in cultured human ARPE-19 cells. (A) Micrograph of an ARPE-19 cell depicting apical microvilli (AM) and basal infoldings (BI). The nucleus (N) can also be seen (7700×). Panel A serves as a comparison for panels B–E, which are higher magnifications of the apical and basal plasma membranes of a RPE cell. (B) Higher magnification of apical microvilli labelled with an antibody against caveolin-1. No gold particles were observed (60,000×). (C) Higher magnification of the basal infoldings of RPE labelled with an antibody against caveolin-1. Arrows point to gold particles (35,500×). (D) Higher magnification of apical microvilli labelled with antibody against the Na + ,K + -ATPase. Gold particles were abundant (46,000×). (E) Higher magnification of the basolateral infoldings of A RPE cells labelled with an antibody against folate receptor α . Gold particles were present across the membrane (46,000×).
Figure Legend Snippet: Electron microscopical analysis of caveolin-1 in cultured human ARPE-19 cells. (A) Micrograph of an ARPE-19 cell depicting apical microvilli (AM) and basal infoldings (BI). The nucleus (N) can also be seen (7700×). Panel A serves as a comparison for panels B–E, which are higher magnifications of the apical and basal plasma membranes of a RPE cell. (B) Higher magnification of apical microvilli labelled with an antibody against caveolin-1. No gold particles were observed (60,000×). (C) Higher magnification of the basal infoldings of RPE labelled with an antibody against caveolin-1. Arrows point to gold particles (35,500×). (D) Higher magnification of apical microvilli labelled with antibody against the Na + ,K + -ATPase. Gold particles were abundant (46,000×). (E) Higher magnification of the basolateral infoldings of A RPE cells labelled with an antibody against folate receptor α . Gold particles were present across the membrane (46,000×).

Techniques Used: Cell Culture

Laser scanning confocal microscopic localization of caveolin-1 and folate receptor α in intact mouse RPE. (A) High magnification of mouse retina incubated with an antibody against caveolin-1. The apical and basal plasma membranes of the RPE are represented by the top (a) and bottom (b) two-headed arrows, respectively. Caveolin-1 is present on the apical (arrows) and basal (arrowheads) plasmalemmal surfaces of the RPE cell. (B) High magnification of mouse retina incubated with an antibody against the folate receptor α . Note the intense bands of fluorescence on the basolateral membrane of RPE, but no immunoreactivity in the apical region. (C) High magnification of mouse retina incubated with an antibody against the Na + ,K + -ATPase. As expected, an intense band of fluorescent labelling was observed on the apical surface of the RPE monolayer. (D) Haematoxylin and eosin-stained section of mouse retina shown for comparison (630×). Abbreviations: IS, inner segments; OS, outer segments; RPE, retinal pigment epithelium.
Figure Legend Snippet: Laser scanning confocal microscopic localization of caveolin-1 and folate receptor α in intact mouse RPE. (A) High magnification of mouse retina incubated with an antibody against caveolin-1. The apical and basal plasma membranes of the RPE are represented by the top (a) and bottom (b) two-headed arrows, respectively. Caveolin-1 is present on the apical (arrows) and basal (arrowheads) plasmalemmal surfaces of the RPE cell. (B) High magnification of mouse retina incubated with an antibody against the folate receptor α . Note the intense bands of fluorescence on the basolateral membrane of RPE, but no immunoreactivity in the apical region. (C) High magnification of mouse retina incubated with an antibody against the Na + ,K + -ATPase. As expected, an intense band of fluorescent labelling was observed on the apical surface of the RPE monolayer. (D) Haematoxylin and eosin-stained section of mouse retina shown for comparison (630×). Abbreviations: IS, inner segments; OS, outer segments; RPE, retinal pigment epithelium.

Techniques Used: Incubation, Fluorescence, Staining

Electron microscopical immunohistochemical analysis of caveolin-1 in mammalian retina. (A) Mouse RPE depicting apical microvilli (AM) and basal infoldings (BI). The nucleus (N) and mitochondria (M) are also visible (7700×). Panel A serves as a comparison for panels B–E, which are higher magnifications of the apical and basal plasma membranes of a RPE cell. (B) Higher magnification of apical microvilli labelled with an antibody against caveolin-1. Arrows point to gold particles indicative of positive labelling (27,500×). (C) Higher magnification of the basal infoldings of RPE labelled with an antibody against caveolin-1. Arrows point to gold particles (35,500×). (D) Higher magnification of apical microvilli labelled with antibody against the Na + ,K + -ATPase (60,000×). Gold particles were abundant. (E) Higher magnification of the basolateral infoldings of a RPE cell labelled with an antibody against folate receptor α (60,000×). Gold particles were present across the membrane.
Figure Legend Snippet: Electron microscopical immunohistochemical analysis of caveolin-1 in mammalian retina. (A) Mouse RPE depicting apical microvilli (AM) and basal infoldings (BI). The nucleus (N) and mitochondria (M) are also visible (7700×). Panel A serves as a comparison for panels B–E, which are higher magnifications of the apical and basal plasma membranes of a RPE cell. (B) Higher magnification of apical microvilli labelled with an antibody against caveolin-1. Arrows point to gold particles indicative of positive labelling (27,500×). (C) Higher magnification of the basal infoldings of RPE labelled with an antibody against caveolin-1. Arrows point to gold particles (35,500×). (D) Higher magnification of apical microvilli labelled with antibody against the Na + ,K + -ATPase (60,000×). Gold particles were abundant. (E) Higher magnification of the basolateral infoldings of a RPE cell labelled with an antibody against folate receptor α (60,000×). Gold particles were present across the membrane.

Techniques Used: Immunohistochemistry

Related Articles

Marker:

Article Title: A comparison of caveolae and caveolin-1 to folate receptor α in retina and retinal pigment epithelium
Article Snippet: .. The Na+ ,K+ -ATPase was used as a marker for the apical plasma membrane of the RPE cells ( , , , , ). .. Folate receptor α served as a marker for the basolateral plasmalemmal surface of the RPE ( , ).

Incubation:

Article Title: A comparison of caveolae and caveolin-1 to folate receptor α in retina and retinal pigment epithelium
Article Snippet: .. After blocking with 4% normal goat serum, the sections were incubated for 6 h at room temperature with a polyclonal IgG against caveolin-1 (1 : 100), a polyclonal IgG against folate receptor α (1 : 50), or a polyclonal antibody against Na+ ,K+ -ATPase (1 : 50). .. Sections were rinsed in PBS and incubated for 1 h at room temperature with an 18 nm anti-rabbit colloidal gold conjugated IgG at a dilution of 1 : 100 (Jackson ImmunoResearch Laboratories).

Article Title: A comparison of caveolae and caveolin-1 to folate receptor α in retina and retinal pigment epithelium
Article Snippet: .. Cells were incubated overnight at 4 °C with either an antibody against caveolin-1 at a dilution of 1 : 100 or an antibody against the Na+ ,K+ -ATPase at a dilution of 1 : 50. .. After washing, cells were incubated overnight at 4 °C with a FITC-conjugated IgG antibody.

other:

Article Title: A comparison of caveolae and caveolin-1 to folate receptor α in retina and retinal pigment epithelium
Article Snippet: To rule out the possibility that the positive basolateral labelling was due to autofluorescence of Bruch’s membrane or RPE, we performed immunolabelling studies of the Na+ ,K+ -ATPase as well.

Article Title: A comparison of caveolae and caveolin-1 to folate receptor α in retina and retinal pigment epithelium
Article Snippet: The Na+ ,K+ -ATPase was localized to the apical microvilli ( ) while folate receptor α was localized to the basolateral infoldings ( ).

Article Title: A comparison of caveolae and caveolin-1 to folate receptor α in retina and retinal pigment epithelium
Article Snippet: As positive controls, folate receptor α and the Na+ ,K+ -ATPase were localized in the RPE.

Article Title: A comparison of caveolae and caveolin-1 to folate receptor α in retina and retinal pigment epithelium
Article Snippet: The Na+ ,K+ -ATPase was expressed abundantly on the apical microvilli ( ) while folate receptor α was detected only on the basolateral infoldings ( ).

Blocking Assay:

Article Title: A comparison of caveolae and caveolin-1 to folate receptor α in retina and retinal pigment epithelium
Article Snippet: .. After blocking with 4% normal goat serum, the sections were incubated for 6 h at room temperature with a polyclonal IgG against caveolin-1 (1 : 100), a polyclonal IgG against folate receptor α (1 : 50), or a polyclonal antibody against Na+ ,K+ -ATPase (1 : 50). .. Sections were rinsed in PBS and incubated for 1 h at room temperature with an 18 nm anti-rabbit colloidal gold conjugated IgG at a dilution of 1 : 100 (Jackson ImmunoResearch Laboratories).

Positive Control:

Article Title: A comparison of caveolae and caveolin-1 to folate receptor α in retina and retinal pigment epithelium
Article Snippet: .. As a positive control, the Na+ ,K+ -ATPase and folate receptor α were localized in these cells. .. The Na+ ,K+ -ATPase was localized to the apical microvilli ( ) while folate receptor α was localized to the basolateral infoldings ( ).

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    Accurate Chemical & Scientific Corporation k atpase
    Electron microscopical analysis of caveolin-1 in cultured human ARPE-19 cells. (A) Micrograph of an ARPE-19 cell depicting apical microvilli (AM) and basal infoldings (BI). The nucleus (N) can also be seen (7700×). Panel A serves as a comparison for panels B–E, which are higher magnifications of the apical and basal plasma membranes of a RPE cell. (B) Higher magnification of apical microvilli labelled with an antibody against caveolin-1. No gold particles were observed (60,000×). (C) Higher magnification of the basal infoldings of RPE labelled with an antibody against caveolin-1. Arrows point to gold particles (35,500×). (D) Higher magnification of apical microvilli labelled with antibody against the Na + ,K + <t>-ATPase.</t> Gold particles were abundant (46,000×). (E) Higher magnification of the basolateral infoldings of A RPE cells labelled with an antibody against folate receptor <t>α</t> . Gold particles were present across the membrane (46,000×).
    K Atpase, supplied by Accurate Chemical & Scientific Corporation, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Electron microscopical analysis of caveolin-1 in cultured human ARPE-19 cells. (A) Micrograph of an ARPE-19 cell depicting apical microvilli (AM) and basal infoldings (BI). The nucleus (N) can also be seen (7700×). Panel A serves as a comparison for panels B–E, which are higher magnifications of the apical and basal plasma membranes of a RPE cell. (B) Higher magnification of apical microvilli labelled with an antibody against caveolin-1. No gold particles were observed (60,000×). (C) Higher magnification of the basal infoldings of RPE labelled with an antibody against caveolin-1. Arrows point to gold particles (35,500×). (D) Higher magnification of apical microvilli labelled with antibody against the Na + ,K + -ATPase. Gold particles were abundant (46,000×). (E) Higher magnification of the basolateral infoldings of A RPE cells labelled with an antibody against folate receptor α . Gold particles were present across the membrane (46,000×).

    Journal: The Histochemical journal

    Article Title: A comparison of caveolae and caveolin-1 to folate receptor α in retina and retinal pigment epithelium

    doi:

    Figure Lengend Snippet: Electron microscopical analysis of caveolin-1 in cultured human ARPE-19 cells. (A) Micrograph of an ARPE-19 cell depicting apical microvilli (AM) and basal infoldings (BI). The nucleus (N) can also be seen (7700×). Panel A serves as a comparison for panels B–E, which are higher magnifications of the apical and basal plasma membranes of a RPE cell. (B) Higher magnification of apical microvilli labelled with an antibody against caveolin-1. No gold particles were observed (60,000×). (C) Higher magnification of the basal infoldings of RPE labelled with an antibody against caveolin-1. Arrows point to gold particles (35,500×). (D) Higher magnification of apical microvilli labelled with antibody against the Na + ,K + -ATPase. Gold particles were abundant (46,000×). (E) Higher magnification of the basolateral infoldings of A RPE cells labelled with an antibody against folate receptor α . Gold particles were present across the membrane (46,000×).

    Article Snippet: After blocking with 4% normal goat serum, the sections were incubated for 6 h at room temperature with a polyclonal IgG against caveolin-1 (1 : 100), a polyclonal IgG against folate receptor α (1 : 50), or a polyclonal antibody against Na+ ,K+ -ATPase (1 : 50).

    Techniques: Cell Culture

    Laser scanning confocal microscopic localization of caveolin-1 and folate receptor α in intact mouse RPE. (A) High magnification of mouse retina incubated with an antibody against caveolin-1. The apical and basal plasma membranes of the RPE are represented by the top (a) and bottom (b) two-headed arrows, respectively. Caveolin-1 is present on the apical (arrows) and basal (arrowheads) plasmalemmal surfaces of the RPE cell. (B) High magnification of mouse retina incubated with an antibody against the folate receptor α . Note the intense bands of fluorescence on the basolateral membrane of RPE, but no immunoreactivity in the apical region. (C) High magnification of mouse retina incubated with an antibody against the Na + ,K + -ATPase. As expected, an intense band of fluorescent labelling was observed on the apical surface of the RPE monolayer. (D) Haematoxylin and eosin-stained section of mouse retina shown for comparison (630×). Abbreviations: IS, inner segments; OS, outer segments; RPE, retinal pigment epithelium.

    Journal: The Histochemical journal

    Article Title: A comparison of caveolae and caveolin-1 to folate receptor α in retina and retinal pigment epithelium

    doi:

    Figure Lengend Snippet: Laser scanning confocal microscopic localization of caveolin-1 and folate receptor α in intact mouse RPE. (A) High magnification of mouse retina incubated with an antibody against caveolin-1. The apical and basal plasma membranes of the RPE are represented by the top (a) and bottom (b) two-headed arrows, respectively. Caveolin-1 is present on the apical (arrows) and basal (arrowheads) plasmalemmal surfaces of the RPE cell. (B) High magnification of mouse retina incubated with an antibody against the folate receptor α . Note the intense bands of fluorescence on the basolateral membrane of RPE, but no immunoreactivity in the apical region. (C) High magnification of mouse retina incubated with an antibody against the Na + ,K + -ATPase. As expected, an intense band of fluorescent labelling was observed on the apical surface of the RPE monolayer. (D) Haematoxylin and eosin-stained section of mouse retina shown for comparison (630×). Abbreviations: IS, inner segments; OS, outer segments; RPE, retinal pigment epithelium.

    Article Snippet: After blocking with 4% normal goat serum, the sections were incubated for 6 h at room temperature with a polyclonal IgG against caveolin-1 (1 : 100), a polyclonal IgG against folate receptor α (1 : 50), or a polyclonal antibody against Na+ ,K+ -ATPase (1 : 50).

    Techniques: Incubation, Fluorescence, Staining

    Electron microscopical immunohistochemical analysis of caveolin-1 in mammalian retina. (A) Mouse RPE depicting apical microvilli (AM) and basal infoldings (BI). The nucleus (N) and mitochondria (M) are also visible (7700×). Panel A serves as a comparison for panels B–E, which are higher magnifications of the apical and basal plasma membranes of a RPE cell. (B) Higher magnification of apical microvilli labelled with an antibody against caveolin-1. Arrows point to gold particles indicative of positive labelling (27,500×). (C) Higher magnification of the basal infoldings of RPE labelled with an antibody against caveolin-1. Arrows point to gold particles (35,500×). (D) Higher magnification of apical microvilli labelled with antibody against the Na + ,K + -ATPase (60,000×). Gold particles were abundant. (E) Higher magnification of the basolateral infoldings of a RPE cell labelled with an antibody against folate receptor α (60,000×). Gold particles were present across the membrane.

    Journal: The Histochemical journal

    Article Title: A comparison of caveolae and caveolin-1 to folate receptor α in retina and retinal pigment epithelium

    doi:

    Figure Lengend Snippet: Electron microscopical immunohistochemical analysis of caveolin-1 in mammalian retina. (A) Mouse RPE depicting apical microvilli (AM) and basal infoldings (BI). The nucleus (N) and mitochondria (M) are also visible (7700×). Panel A serves as a comparison for panels B–E, which are higher magnifications of the apical and basal plasma membranes of a RPE cell. (B) Higher magnification of apical microvilli labelled with an antibody against caveolin-1. Arrows point to gold particles indicative of positive labelling (27,500×). (C) Higher magnification of the basal infoldings of RPE labelled with an antibody against caveolin-1. Arrows point to gold particles (35,500×). (D) Higher magnification of apical microvilli labelled with antibody against the Na + ,K + -ATPase (60,000×). Gold particles were abundant. (E) Higher magnification of the basolateral infoldings of a RPE cell labelled with an antibody against folate receptor α (60,000×). Gold particles were present across the membrane.

    Article Snippet: After blocking with 4% normal goat serum, the sections were incubated for 6 h at room temperature with a polyclonal IgG against caveolin-1 (1 : 100), a polyclonal IgG against folate receptor α (1 : 50), or a polyclonal antibody against Na+ ,K+ -ATPase (1 : 50).

    Techniques: Immunohistochemistry