Structured Review

Millipore jm109
Effects of different chaperones on HPV His 6 -E6 expression levels. Representative immunoblots of induction and purification of HPV16 His 6 -E6 produced in E. coli <t>JM109.</t> a Bacterial transformation with the pQE30-HPV16 His 6 -E6 expression plasmid alone (w/o) or with the different chaperone systems (chaperones A–E, see Table 2 ). Total proteins were extracted by re-suspension in SDS-loading buffer of E. coli cell cultures, according to their OD 600 . b Purification of the HPV16 His 6 -E6 protein without chaperones (w/o) and with the trigger factor chaperone (with chaperones; chaperone E, see Table 2 ). M ColorBurst, Sigma, lanes 1 1 µL, lanes 2 3 µL, lane 3 C + , 20 ng of purified His 6 -E6 protein positive control
Jm109, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/jm109/product/Millipore
Average 99 stars, based on 13 article reviews
Price from $9.99 to $1999.99
jm109 - by Bioz Stars, 2020-04
99/100 stars

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1) Product Images from "Production of functional, stable, unmutated recombinant human papillomavirus E6 oncoprotein: implications for HPV-tumor diagnosis and therapy"

Article Title: Production of functional, stable, unmutated recombinant human papillomavirus E6 oncoprotein: implications for HPV-tumor diagnosis and therapy

Journal: Journal of Translational Medicine

doi: 10.1186/s12967-016-0978-6

Effects of different chaperones on HPV His 6 -E6 expression levels. Representative immunoblots of induction and purification of HPV16 His 6 -E6 produced in E. coli JM109. a Bacterial transformation with the pQE30-HPV16 His 6 -E6 expression plasmid alone (w/o) or with the different chaperone systems (chaperones A–E, see Table 2 ). Total proteins were extracted by re-suspension in SDS-loading buffer of E. coli cell cultures, according to their OD 600 . b Purification of the HPV16 His 6 -E6 protein without chaperones (w/o) and with the trigger factor chaperone (with chaperones; chaperone E, see Table 2 ). M ColorBurst, Sigma, lanes 1 1 µL, lanes 2 3 µL, lane 3 C + , 20 ng of purified His 6 -E6 protein positive control
Figure Legend Snippet: Effects of different chaperones on HPV His 6 -E6 expression levels. Representative immunoblots of induction and purification of HPV16 His 6 -E6 produced in E. coli JM109. a Bacterial transformation with the pQE30-HPV16 His 6 -E6 expression plasmid alone (w/o) or with the different chaperone systems (chaperones A–E, see Table 2 ). Total proteins were extracted by re-suspension in SDS-loading buffer of E. coli cell cultures, according to their OD 600 . b Purification of the HPV16 His 6 -E6 protein without chaperones (w/o) and with the trigger factor chaperone (with chaperones; chaperone E, see Table 2 ). M ColorBurst, Sigma, lanes 1 1 µL, lanes 2 3 µL, lane 3 C + , 20 ng of purified His 6 -E6 protein positive control

Techniques Used: Expressing, Western Blot, Purification, Produced, Electroporation Bacterial Transformation, Plasmid Preparation, Positive Control

Related Articles

Clone Assay:

Article Title: Mitochondrial ribosomal proteins involved in tellurite resistance in yeast Saccharomyces cerevisiae
Article Snippet: The origin of strains BY4741, BY4742, BY4743, BY4743-20811, BY4743-27248, BY4743-22461, BY4741-811 and BY4741-2461 is the Yeast Knockout Clones and Collection of the DharmaconTM , and were provided by Carlo Erba s.r.l. (Italy). .. E. coli JM109 competent cells (Sigma-Aldrich) were used for transformation experiments.

Article Title: Hybrid Pathogenicity Island PAGI-5 Contributes to the Highly Virulent Phenotype of a Pseudomonas aeruginosa Isolate in Mammals ▿ Isolate in Mammals ▿ †
Article Snippet: Subtractive hybridization products were ligated to the pGEM-T T/A cloning vector (Promega) at 4°C overnight ( ). .. Transformation was performed by adding 2 μl of a ligation mixture to JM109 competent cells , and transformants were selected for by growth on LB agar supplemented with ampicillin (50 μg/μl), isopropyl-β- d -thiogalactopyranoside (IPTG) (50 μg/μl), and 5-bromo-4-chloro-3-indolyl-β- d -galactopyranoside (X-Gal) (50 μg/μl; Sigma-Aldrich, St. Louis, MO).

Article Title: A promoter-proximal transcript targeted by genetic polymorphism controls E-cadherin silencing in human cancers
Article Snippet: Paragraph title: Cloning and site-directed mutagenesis ... Plasmids were amplified in JM109 or DH5α competent cells and purified by GenElute Plasmid Miniprep Kit (Sigma).

Amplification:

Article Title: Sexual Phenotype Differences in zic2 mRNA Abundance in the Preoptic Area of a Protogynous Teleost, Thalassoma bifasciatum
Article Snippet: Zic2 cDNA was amplified from a plasmid isolated from this cDNA library PCR product size was estimated on a 1% agarose gel and this product was purified with a QIAquick PCR Purification Kit (Qiagen, Valencia, CA), and sequenced. .. Purified PCR product was transformed into JM109 competent cells (Sigma, St. Louis, MO).

Article Title: A promoter-proximal transcript targeted by genetic polymorphism controls E-cadherin silencing in human cancers
Article Snippet: .. Plasmids were amplified in JM109 or DH5α competent cells and purified by GenElute Plasmid Miniprep Kit (Sigma). .. DNA sequences of all constructs were confirmed by DNA sequencing.

Construct:

Article Title: A promoter-proximal transcript targeted by genetic polymorphism controls E-cadherin silencing in human cancers
Article Snippet: Plasmids were amplified in JM109 or DH5α competent cells and purified by GenElute Plasmid Miniprep Kit (Sigma). .. DNA sequences of all constructs were confirmed by DNA sequencing.

Article Title: Intracellular spectral recompositioning of light enhances algal photosynthetic efficiency
Article Snippet: .. The construct pPha-NR/eGFP was then transformed into Escherichia coli (JM109) competent cells from Sigma-Aldrich. .. After selection with ampicillin (100 μg/ml), the construct pPha-NR/eGFP was then extracted from the cell culture using the Qiagen Plasmid Mini kit from Qiagen.

Electrophoresis:

Article Title: Hybrid Pathogenicity Island PAGI-5 Contributes to the Highly Virulent Phenotype of a Pseudomonas aeruginosa Isolate in Mammals ▿ Isolate in Mammals ▿ †
Article Snippet: Transformation was performed by adding 2 μl of a ligation mixture to JM109 competent cells , and transformants were selected for by growth on LB agar supplemented with ampicillin (50 μg/μl), isopropyl-β- d -thiogalactopyranoside (IPTG) (50 μg/μl), and 5-bromo-4-chloro-3-indolyl-β- d -galactopyranoside (X-Gal) (50 μg/μl; Sigma-Aldrich, St. Louis, MO). .. Plasmid DNA was digested with BglI for 1 h at 37°C and then screened to determine the presence of an insert and the insert size following electrophoresis through a 0.8% agarose gel.

Microarray:

Article Title: Sexual Phenotype Differences in zic2 mRNA Abundance in the Preoptic Area of a Protogynous Teleost, Thalassoma bifasciatum
Article Snippet: Preparation of riboprobe template DNA Previous microarray work in our lab generated a bluehead wrasse brain cDNA library (pBluescript vector; Stratagene, La Jolla, CA) from field-collected fish. .. Purified PCR product was transformed into JM109 competent cells (Sigma, St. Louis, MO).

Transformation Assay:

Article Title: Sexual Phenotype Differences in zic2 mRNA Abundance in the Preoptic Area of a Protogynous Teleost, Thalassoma bifasciatum
Article Snippet: .. Purified PCR product was transformed into JM109 competent cells (Sigma, St. Louis, MO). .. Upon confirmation of the zic2 insert, plasmid was either linearized with ECOR1 for antisense template or HINDIII for sense template.

Article Title: Mitochondrial ribosomal proteins involved in tellurite resistance in yeast Saccharomyces cerevisiae
Article Snippet: .. E. coli JM109 competent cells (Sigma-Aldrich) were used for transformation experiments. ..

Article Title: Hybrid Pathogenicity Island PAGI-5 Contributes to the Highly Virulent Phenotype of a Pseudomonas aeruginosa Isolate in Mammals ▿ Isolate in Mammals ▿ †
Article Snippet: .. Transformation was performed by adding 2 μl of a ligation mixture to JM109 competent cells , and transformants were selected for by growth on LB agar supplemented with ampicillin (50 μg/μl), isopropyl-β- d -thiogalactopyranoside (IPTG) (50 μg/μl), and 5-bromo-4-chloro-3-indolyl-β- d -galactopyranoside (X-Gal) (50 μg/μl; Sigma-Aldrich, St. Louis, MO). .. Following growth in LB broth supplemented with ampicillin (50 μg/μl), plasmid DNA was purified from selected transformants using a spin column technique (Qiagen).

Article Title: Intracellular spectral recompositioning of light enhances algal photosynthetic efficiency
Article Snippet: .. The construct pPha-NR/eGFP was then transformed into Escherichia coli (JM109) competent cells from Sigma-Aldrich. .. After selection with ampicillin (100 μg/ml), the construct pPha-NR/eGFP was then extracted from the cell culture using the Qiagen Plasmid Mini kit from Qiagen.

Genetically Modified:

Article Title: Intracellular spectral recompositioning of light enhances algal photosynthetic efficiency
Article Snippet: Paragraph title: Generation of genetically modified P. tricornutum cells ... The construct pPha-NR/eGFP was then transformed into Escherichia coli (JM109) competent cells from Sigma-Aldrich.

Hybridization:

Article Title: Hybrid Pathogenicity Island PAGI-5 Contributes to the Highly Virulent Phenotype of a Pseudomonas aeruginosa Isolate in Mammals ▿ Isolate in Mammals ▿ †
Article Snippet: Paragraph title: Generation of the subtractive hybridization library. ... Transformation was performed by adding 2 μl of a ligation mixture to JM109 competent cells , and transformants were selected for by growth on LB agar supplemented with ampicillin (50 μg/μl), isopropyl-β- d -thiogalactopyranoside (IPTG) (50 μg/μl), and 5-bromo-4-chloro-3-indolyl-β- d -galactopyranoside (X-Gal) (50 μg/μl; Sigma-Aldrich, St. Louis, MO).

Electroporation:

Article Title: Intracellular spectral recompositioning of light enhances algal photosynthetic efficiency
Article Snippet: The construct pPha-NR/eGFP was then transformed into Escherichia coli (JM109) competent cells from Sigma-Aldrich. .. For transformation of different constructs with eGFP gene to the diatom P. tricornutum , the multipulse electroporation protocol ( ) was followed with slight modifications on the washing step to adjust the resistance of mixture in the cuvette.

Ligation:

Article Title: Hybrid Pathogenicity Island PAGI-5 Contributes to the Highly Virulent Phenotype of a Pseudomonas aeruginosa Isolate in Mammals ▿ Isolate in Mammals ▿ †
Article Snippet: .. Transformation was performed by adding 2 μl of a ligation mixture to JM109 competent cells , and transformants were selected for by growth on LB agar supplemented with ampicillin (50 μg/μl), isopropyl-β- d -thiogalactopyranoside (IPTG) (50 μg/μl), and 5-bromo-4-chloro-3-indolyl-β- d -galactopyranoside (X-Gal) (50 μg/μl; Sigma-Aldrich, St. Louis, MO). .. Following growth in LB broth supplemented with ampicillin (50 μg/μl), plasmid DNA was purified from selected transformants using a spin column technique (Qiagen).

Cell Culture:

Article Title: Intracellular spectral recompositioning of light enhances algal photosynthetic efficiency
Article Snippet: The construct pPha-NR/eGFP was then transformed into Escherichia coli (JM109) competent cells from Sigma-Aldrich. .. After selection with ampicillin (100 μg/ml), the construct pPha-NR/eGFP was then extracted from the cell culture using the Qiagen Plasmid Mini kit from Qiagen.

Generated:

Article Title: Sexual Phenotype Differences in zic2 mRNA Abundance in the Preoptic Area of a Protogynous Teleost, Thalassoma bifasciatum
Article Snippet: Preparation of riboprobe template DNA Previous microarray work in our lab generated a bluehead wrasse brain cDNA library (pBluescript vector; Stratagene, La Jolla, CA) from field-collected fish. .. Purified PCR product was transformed into JM109 competent cells (Sigma, St. Louis, MO).

DNA Sequencing:

Article Title: Hybrid Pathogenicity Island PAGI-5 Contributes to the Highly Virulent Phenotype of a Pseudomonas aeruginosa Isolate in Mammals ▿ Isolate in Mammals ▿ †
Article Snippet: Transformation was performed by adding 2 μl of a ligation mixture to JM109 competent cells , and transformants were selected for by growth on LB agar supplemented with ampicillin (50 μg/μl), isopropyl-β- d -thiogalactopyranoside (IPTG) (50 μg/μl), and 5-bromo-4-chloro-3-indolyl-β- d -galactopyranoside (X-Gal) (50 μg/μl; Sigma-Aldrich, St. Louis, MO). .. Plasmids containing inserts were sequenced by the University of Chicago Cancer Research Center DNA Sequencing Facility (Chicago, IL).

Article Title: A promoter-proximal transcript targeted by genetic polymorphism controls E-cadherin silencing in human cancers
Article Snippet: Plasmids were amplified in JM109 or DH5α competent cells and purified by GenElute Plasmid Miniprep Kit (Sigma). .. DNA sequences of all constructs were confirmed by DNA sequencing.

Polymerase Chain Reaction:

Article Title: Sexual Phenotype Differences in zic2 mRNA Abundance in the Preoptic Area of a Protogynous Teleost, Thalassoma bifasciatum
Article Snippet: .. Purified PCR product was transformed into JM109 competent cells (Sigma, St. Louis, MO). .. Upon confirmation of the zic2 insert, plasmid was either linearized with ECOR1 for antisense template or HINDIII for sense template.

Article Title: Mitochondrial ribosomal proteins involved in tellurite resistance in yeast Saccharomyces cerevisiae
Article Snippet: The yeast knock-out mutants were obtained by using a PCR based strategy to replace each ORF with a kanMX cassette . .. E. coli JM109 competent cells (Sigma-Aldrich) were used for transformation experiments.

Article Title: A promoter-proximal transcript targeted by genetic polymorphism controls E-cadherin silencing in human cancers
Article Snippet: To generate the pGL3-CDH1-TS promoter vector the transcription termination sequence (SV40 polyA site) in the pGL3-basic plasmid was PCR amplified and cloned in the ApaI-EcoRI sites of pGL3-CDH1-T1 promoter vector. .. Plasmids were amplified in JM109 or DH5α competent cells and purified by GenElute Plasmid Miniprep Kit (Sigma).

Article Title: Intracellular spectral recompositioning of light enhances algal photosynthetic efficiency
Article Snippet: The polymerase chain reaction (PCR) products and the vector pPha-NR were then digested respectively using the restriction enzymes Eco RI and Hind III (New England Biolabs Inc.) according to the manufacturer’s manual. .. The construct pPha-NR/eGFP was then transformed into Escherichia coli (JM109) competent cells from Sigma-Aldrich.

Mutagenesis:

Article Title: A promoter-proximal transcript targeted by genetic polymorphism controls E-cadherin silencing in human cancers
Article Snippet: Paragraph title: Cloning and site-directed mutagenesis ... Plasmids were amplified in JM109 or DH5α competent cells and purified by GenElute Plasmid Miniprep Kit (Sigma).

Isolation:

Article Title: Sexual Phenotype Differences in zic2 mRNA Abundance in the Preoptic Area of a Protogynous Teleost, Thalassoma bifasciatum
Article Snippet: Zic2 cDNA was amplified from a plasmid isolated from this cDNA library PCR product size was estimated on a 1% agarose gel and this product was purified with a QIAquick PCR Purification Kit (Qiagen, Valencia, CA), and sequenced. .. Purified PCR product was transformed into JM109 competent cells (Sigma, St. Louis, MO).

Purification:

Article Title: Sexual Phenotype Differences in zic2 mRNA Abundance in the Preoptic Area of a Protogynous Teleost, Thalassoma bifasciatum
Article Snippet: .. Purified PCR product was transformed into JM109 competent cells (Sigma, St. Louis, MO). .. Upon confirmation of the zic2 insert, plasmid was either linearized with ECOR1 for antisense template or HINDIII for sense template.

Article Title: Hybrid Pathogenicity Island PAGI-5 Contributes to the Highly Virulent Phenotype of a Pseudomonas aeruginosa Isolate in Mammals ▿ Isolate in Mammals ▿ †
Article Snippet: Transformation was performed by adding 2 μl of a ligation mixture to JM109 competent cells , and transformants were selected for by growth on LB agar supplemented with ampicillin (50 μg/μl), isopropyl-β- d -thiogalactopyranoside (IPTG) (50 μg/μl), and 5-bromo-4-chloro-3-indolyl-β- d -galactopyranoside (X-Gal) (50 μg/μl; Sigma-Aldrich, St. Louis, MO). .. Following growth in LB broth supplemented with ampicillin (50 μg/μl), plasmid DNA was purified from selected transformants using a spin column technique (Qiagen).

Article Title: A promoter-proximal transcript targeted by genetic polymorphism controls E-cadherin silencing in human cancers
Article Snippet: .. Plasmids were amplified in JM109 or DH5α competent cells and purified by GenElute Plasmid Miniprep Kit (Sigma). .. DNA sequences of all constructs were confirmed by DNA sequencing.

Article Title: Intracellular spectral recompositioning of light enhances algal photosynthetic efficiency
Article Snippet: After digestion, the DNA fragments were purified and ligated using T4 DNA ligase to connect pPha-NR with eGFP gene through their cohesive ends according to the manufacturer’s manual. .. The construct pPha-NR/eGFP was then transformed into Escherichia coli (JM109) competent cells from Sigma-Aldrich.

Sequencing:

Article Title: Sexual Phenotype Differences in zic2 mRNA Abundance in the Preoptic Area of a Protogynous Teleost, Thalassoma bifasciatum
Article Snippet: Comparison of the 727 bp sequence using BLAST (NCBI; sequence submission HQ423137) showed greatest sequence similarity to Gasterosteus aculeatus (three-spined stickleback; 91%; BT027912.1) and Danio rerio zic2a (zebrafish; 84%; AF151535.1) and supported the identification as zic2 . .. Purified PCR product was transformed into JM109 competent cells (Sigma, St. Louis, MO).

Article Title: A promoter-proximal transcript targeted by genetic polymorphism controls E-cadherin silencing in human cancers
Article Snippet: To generate the pGL3-CDH1-TS promoter vector the transcription termination sequence (SV40 polyA site) in the pGL3-basic plasmid was PCR amplified and cloned in the ApaI-EcoRI sites of pGL3-CDH1-T1 promoter vector. .. Plasmids were amplified in JM109 or DH5α competent cells and purified by GenElute Plasmid Miniprep Kit (Sigma).

cDNA Library Assay:

Article Title: Sexual Phenotype Differences in zic2 mRNA Abundance in the Preoptic Area of a Protogynous Teleost, Thalassoma bifasciatum
Article Snippet: Zic2 cDNA was amplified from a plasmid isolated from this cDNA library PCR product size was estimated on a 1% agarose gel and this product was purified with a QIAquick PCR Purification Kit (Qiagen, Valencia, CA), and sequenced. .. Purified PCR product was transformed into JM109 competent cells (Sigma, St. Louis, MO).

In Situ Hybridization:

Article Title: Sexual Phenotype Differences in zic2 mRNA Abundance in the Preoptic Area of a Protogynous Teleost, Thalassoma bifasciatum
Article Snippet: Purified PCR product was transformed into JM109 competent cells (Sigma, St. Louis, MO). .. To further assess probe specificity, an alternate 315 bp antisense zic2 template was cut from the same plasmid and used in in situ hybridization.

Plasmid Preparation:

Article Title: PucC and LhaA direct efficient assembly of the light‐harvesting complexes in Rhodobacter sphaeroides
Article Snippet: JM109 chemically competent cells purchased from Sigma and S17‐1 (Simon et al ., ). .. S17‐1 cells were used for plasmid transfer into Rba. sphaeroides strains and were made electrocompetent.

Article Title: Sexual Phenotype Differences in zic2 mRNA Abundance in the Preoptic Area of a Protogynous Teleost, Thalassoma bifasciatum
Article Snippet: Zic2 cDNA was amplified from a plasmid isolated from this cDNA library PCR product size was estimated on a 1% agarose gel and this product was purified with a QIAquick PCR Purification Kit (Qiagen, Valencia, CA), and sequenced. .. Purified PCR product was transformed into JM109 competent cells (Sigma, St. Louis, MO).

Article Title: Hybrid Pathogenicity Island PAGI-5 Contributes to the Highly Virulent Phenotype of a Pseudomonas aeruginosa Isolate in Mammals ▿ Isolate in Mammals ▿ †
Article Snippet: Subtractive hybridization products were ligated to the pGEM-T T/A cloning vector (Promega) at 4°C overnight ( ). .. Transformation was performed by adding 2 μl of a ligation mixture to JM109 competent cells , and transformants were selected for by growth on LB agar supplemented with ampicillin (50 μg/μl), isopropyl-β- d -thiogalactopyranoside (IPTG) (50 μg/μl), and 5-bromo-4-chloro-3-indolyl-β- d -galactopyranoside (X-Gal) (50 μg/μl; Sigma-Aldrich, St. Louis, MO).

Article Title: Assembly of functional photosystem complexes in Rhodobacter sphaeroides incorporating carotenoids from the spirilloxanthin pathway
Article Snippet: 2.1 Escherichia coli strains and plasmids Two strains of E. coli were primarily used in this work; JM109 chemically competent cells purchased from Sigma, and S17-1 . .. S17-1 cells were used for plasmid transfer into Rba. sphaeroides strains and were made electrocompetent.

Article Title: A promoter-proximal transcript targeted by genetic polymorphism controls E-cadherin silencing in human cancers
Article Snippet: .. Plasmids were amplified in JM109 or DH5α competent cells and purified by GenElute Plasmid Miniprep Kit (Sigma). .. DNA sequences of all constructs were confirmed by DNA sequencing.

Article Title: Intracellular spectral recompositioning of light enhances algal photosynthetic efficiency
Article Snippet: The polymerase chain reaction (PCR) products and the vector pPha-NR were then digested respectively using the restriction enzymes Eco RI and Hind III (New England Biolabs Inc.) according to the manufacturer’s manual. .. The construct pPha-NR/eGFP was then transformed into Escherichia coli (JM109) competent cells from Sigma-Aldrich.

Selection:

Article Title: Mitochondrial ribosomal proteins involved in tellurite resistance in yeast Saccharomyces cerevisiae
Article Snippet: When required G418 (200 μg/ml) was for strain selection. .. E. coli JM109 competent cells (Sigma-Aldrich) were used for transformation experiments.

Article Title: Intracellular spectral recompositioning of light enhances algal photosynthetic efficiency
Article Snippet: The construct pPha-NR/eGFP was then transformed into Escherichia coli (JM109) competent cells from Sigma-Aldrich. .. After selection with ampicillin (100 μg/ml), the construct pPha-NR/eGFP was then extracted from the cell culture using the Qiagen Plasmid Mini kit from Qiagen.

Agarose Gel Electrophoresis:

Article Title: Sexual Phenotype Differences in zic2 mRNA Abundance in the Preoptic Area of a Protogynous Teleost, Thalassoma bifasciatum
Article Snippet: Zic2 cDNA was amplified from a plasmid isolated from this cDNA library PCR product size was estimated on a 1% agarose gel and this product was purified with a QIAquick PCR Purification Kit (Qiagen, Valencia, CA), and sequenced. .. Purified PCR product was transformed into JM109 competent cells (Sigma, St. Louis, MO).

Article Title: Hybrid Pathogenicity Island PAGI-5 Contributes to the Highly Virulent Phenotype of a Pseudomonas aeruginosa Isolate in Mammals ▿ Isolate in Mammals ▿ †
Article Snippet: Transformation was performed by adding 2 μl of a ligation mixture to JM109 competent cells , and transformants were selected for by growth on LB agar supplemented with ampicillin (50 μg/μl), isopropyl-β- d -thiogalactopyranoside (IPTG) (50 μg/μl), and 5-bromo-4-chloro-3-indolyl-β- d -galactopyranoside (X-Gal) (50 μg/μl; Sigma-Aldrich, St. Louis, MO). .. Plasmid DNA was digested with BglI for 1 h at 37°C and then screened to determine the presence of an insert and the insert size following electrophoresis through a 0.8% agarose gel.

Knock-Out:

Article Title: Mitochondrial ribosomal proteins involved in tellurite resistance in yeast Saccharomyces cerevisiae
Article Snippet: The yeast knock-out mutants were obtained by using a PCR based strategy to replace each ORF with a kanMX cassette . .. E. coli JM109 competent cells (Sigma-Aldrich) were used for transformation experiments.

Fluorescence In Situ Hybridization:

Article Title: Sexual Phenotype Differences in zic2 mRNA Abundance in the Preoptic Area of a Protogynous Teleost, Thalassoma bifasciatum
Article Snippet: Preparation of riboprobe template DNA Previous microarray work in our lab generated a bluehead wrasse brain cDNA library (pBluescript vector; Stratagene, La Jolla, CA) from field-collected fish. .. Purified PCR product was transformed into JM109 competent cells (Sigma, St. Louis, MO).

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    Millipore e coli jm109
    E Coli Jm109, supplied by Millipore, used in various techniques. Bioz Stars score: 87/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli jm109/product/Millipore
    Average 87 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    e coli jm109 - by Bioz Stars, 2020-04
    87/100 stars
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