Structured Review

Promega iu rnasin
Iu Rnasin, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/iu rnasin/product/Promega
Average 91 stars, based on 1 article reviews
Price from $9.99 to $1999.99
iu rnasin - by Bioz Stars, 2020-04
91/100 stars

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Negative Control:

Article Title: Maternal Nutrition during Pregnancy Affects Testicular and Bone Development, Glucose Metabolism and Response to Overnutrition in Weaned Horses Up to Two Years
Article Snippet: Total RNA (250 ng) was used for reverse transcription with 100 IU Moloney murine leukemia virus reverse transcriptase (Promega, France), 20 IU RNasin (Promega, France), 500 μM dNTPs (Promega, France) in a 20 μl final volume for 90 min at 37°C. .. Negative control contained water and all set primers were designed to be placed on different exons.

Amplification:

Article Title: Electroacupuncture enhances extragonadal aromatization in ovariectomized rats
Article Snippet: RNA analysis Total RNA (2 μg) was transcribed in reverse, in a final volume of 20 μl, using 200 IU M-MLV reverse transcriptase in the presence of 25 pmol downstream primer (Sangon Inc), 0.5 mM deoxy-NTP and 20 IU Rnasin (from Promega) for 60 min at 42°C before heat denatured for 5 min at 95°C. .. The cDNAs obtained were further amplified by PCR using 25 pmol of upstream primer (Sangon Inc) designed to amplify P450 arom highly conserved sequence (289 bp length) including helical and aromatic region, (upstream Aro-Ex8, 5'GCT TCT CAT CGC AGA GTA TCC GG-3' located in the exon 8; downstream Aro-Ex9, 5'-CAA GGG TAA ATT CAT TGG GCT TGG-3' located in the exon 9; amplified product 289 bp) [ ].

Sample Prep:

Article Title: Analysis of androgen receptor and anti-Müllerian hormone pathways in human granulosa cells under luteinizing hormone treatment
Article Snippet: .. cDNA synthesis Two micrograms of total RNA from the sample preparation were reverse-transcribed in 25 μL as follows: 0.1 μg of random hexamer primers (Amersham Pharmacia Biotech, Inc., Buckinghamshire, UK) were denatured for 10 minutes at 70°C in a Gradient Cycler (DNA Engine, Watertown, MA, USA), and then the reverse transcription reaction was performed at 42°C for 1 hour by adding 5× reverse transcriptase buffer (500 mM of each dNTP, 3 mM MgCl2 , 75 mM KCl, and 50 mM Tris-HCl [pH 8.3]), 1 mM dNTP (Promega, Madison, WI, USA), 10 IU RNase inhibitor (Promega), and 100 U MMLV reverse transcriptase (Promega). .. Quantitative RT-PCR Specific PCR amplification products were detected using an ABI PRISM 7700 sequence detector system (Perkin-Elmer Applied Biosystems, Los Angeles, CA, USA) and the SYBR green PCR master mix kit (Applied Biosystems, Foster City, USA), according to the manufacturer’s protocol.

Real-time Polymerase Chain Reaction:

Article Title: Maternal Nutrition during Pregnancy Affects Testicular and Bone Development, Glucose Metabolism and Response to Overnutrition in Weaned Horses Up to Two Years
Article Snippet: Paragraph title: Quantitative real time PCR ... Total RNA (250 ng) was used for reverse transcription with 100 IU Moloney murine leukemia virus reverse transcriptase (Promega, France), 20 IU RNasin (Promega, France), 500 μM dNTPs (Promega, France) in a 20 μl final volume for 90 min at 37°C.

Polymerase Chain Reaction:

Article Title: Maternal Nutrition during Pregnancy Affects Testicular and Bone Development, Glucose Metabolism and Response to Overnutrition in Weaned Horses Up to Two Years
Article Snippet: Total RNA (250 ng) was used for reverse transcription with 100 IU Moloney murine leukemia virus reverse transcriptase (Promega, France), 20 IU RNasin (Promega, France), 500 μM dNTPs (Promega, France) in a 20 μl final volume for 90 min at 37°C. .. PCR reactions were performed in a Stratagene Mx3005P apparatus (Agilent, France).

Article Title: Electroacupuncture enhances extragonadal aromatization in ovariectomized rats
Article Snippet: RNA analysis Total RNA (2 μg) was transcribed in reverse, in a final volume of 20 μl, using 200 IU M-MLV reverse transcriptase in the presence of 25 pmol downstream primer (Sangon Inc), 0.5 mM deoxy-NTP and 20 IU Rnasin (from Promega) for 60 min at 42°C before heat denatured for 5 min at 95°C. .. The cDNAs obtained were further amplified by PCR using 25 pmol of upstream primer (Sangon Inc) designed to amplify P450 arom highly conserved sequence (289 bp length) including helical and aromatic region, (upstream Aro-Ex8, 5'GCT TCT CAT CGC AGA GTA TCC GG-3' located in the exon 8; downstream Aro-Ex9, 5'-CAA GGG TAA ATT CAT TGG GCT TGG-3' located in the exon 9; amplified product 289 bp) [ ].

Random Hexamer Labeling:

Article Title: Analysis of androgen receptor and anti-Müllerian hormone pathways in human granulosa cells under luteinizing hormone treatment
Article Snippet: .. cDNA synthesis Two micrograms of total RNA from the sample preparation were reverse-transcribed in 25 μL as follows: 0.1 μg of random hexamer primers (Amersham Pharmacia Biotech, Inc., Buckinghamshire, UK) were denatured for 10 minutes at 70°C in a Gradient Cycler (DNA Engine, Watertown, MA, USA), and then the reverse transcription reaction was performed at 42°C for 1 hour by adding 5× reverse transcriptase buffer (500 mM of each dNTP, 3 mM MgCl2 , 75 mM KCl, and 50 mM Tris-HCl [pH 8.3]), 1 mM dNTP (Promega, Madison, WI, USA), 10 IU RNase inhibitor (Promega), and 100 U MMLV reverse transcriptase (Promega). .. Quantitative RT-PCR Specific PCR amplification products were detected using an ABI PRISM 7700 sequence detector system (Perkin-Elmer Applied Biosystems, Los Angeles, CA, USA) and the SYBR green PCR master mix kit (Applied Biosystems, Foster City, USA), according to the manufacturer’s protocol.

Expressing:

Article Title: Electroacupuncture enhances extragonadal aromatization in ovariectomized rats
Article Snippet: RNA analysis Total RNA (2 μg) was transcribed in reverse, in a final volume of 20 μl, using 200 IU M-MLV reverse transcriptase in the presence of 25 pmol downstream primer (Sangon Inc), 0.5 mM deoxy-NTP and 20 IU Rnasin (from Promega) for 60 min at 42°C before heat denatured for 5 min at 95°C. .. For P450 arom, we used 35 PCR amplification cycles, and 20 cycles for β-actin gene expression.

Sequencing:

Article Title: Electroacupuncture enhances extragonadal aromatization in ovariectomized rats
Article Snippet: RNA analysis Total RNA (2 μg) was transcribed in reverse, in a final volume of 20 μl, using 200 IU M-MLV reverse transcriptase in the presence of 25 pmol downstream primer (Sangon Inc), 0.5 mM deoxy-NTP and 20 IU Rnasin (from Promega) for 60 min at 42°C before heat denatured for 5 min at 95°C. .. The cDNAs obtained were further amplified by PCR using 25 pmol of upstream primer (Sangon Inc) designed to amplify P450 arom highly conserved sequence (289 bp length) including helical and aromatic region, (upstream Aro-Ex8, 5'GCT TCT CAT CGC AGA GTA TCC GG-3' located in the exon 8; downstream Aro-Ex9, 5'-CAA GGG TAA ATT CAT TGG GCT TGG-3' located in the exon 9; amplified product 289 bp) [ ].

Chloramphenicol Acetyltransferase Assay:

Article Title: Electroacupuncture enhances extragonadal aromatization in ovariectomized rats
Article Snippet: RNA analysis Total RNA (2 μg) was transcribed in reverse, in a final volume of 20 μl, using 200 IU M-MLV reverse transcriptase in the presence of 25 pmol downstream primer (Sangon Inc), 0.5 mM deoxy-NTP and 20 IU Rnasin (from Promega) for 60 min at 42°C before heat denatured for 5 min at 95°C. .. The cDNAs obtained were further amplified by PCR using 25 pmol of upstream primer (Sangon Inc) designed to amplify P450 arom highly conserved sequence (289 bp length) including helical and aromatic region, (upstream Aro-Ex8, 5'GCT TCT CAT CGC AGA GTA TCC GG-3' located in the exon 8; downstream Aro-Ex9, 5'-CAA GGG TAA ATT CAT TGG GCT TGG-3' located in the exon 9; amplified product 289 bp) [ ].

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    Promega iu rnasin
    Iu Rnasin, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/iu rnasin/product/Promega
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    iu rnasin - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

    99
    Promega 120 iu rnasin ribonuclease inhibitor
    120 Iu Rnasin Ribonuclease Inhibitor, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/120 iu rnasin ribonuclease inhibitor/product/Promega
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    120 iu rnasin ribonuclease inhibitor - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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