datp  (New England Biolabs)


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  • 99
    Name:
    dATP Solution
    Description:
    dATP Solution 25 umol
    Catalog Number:
    n0440s
    Price:
    54
    Size:
    25 umol
    Category:
    Deoxynucleotides
    Buy from Supplier


    Structured Review

    New England Biolabs datp
    dATP Solution
    dATP Solution 25 umol
    https://www.bioz.com/result/datp/product/New England Biolabs
    Average 99 stars, based on 5277 article reviews
    Price from $9.99 to $1999.99
    datp - by Bioz Stars, 2020-05
    99/100 stars

    Images

    1) Product Images from "Characterization and evolutionary history of an archaeal kinase involved in selenocysteinyl-tRNA formation"

    Article Title: Characterization and evolutionary history of an archaeal kinase involved in selenocysteinyl-tRNA formation

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkm1134

    Specificity of M. jannaschii PSTK for the phosphoryl donor. Initial velocities for the phosphotransferase activity of PSTK (6 nM PSTK without NTP or the addition of ATP, ITP, GTP, CTP, UTP or dATP or 200 nM PSTK with AMP-CPP or AMP-PCP) in the presence of the indicated phosphoryl donors (20 mM). Reactions proceeded for 6 min at 37°C and 1.5 min time points were taken and analyzed as in Figure 1 A and 2 . *ND, no detectable phosphorylation. Error bars represent the standard deviation of three experiments.
    Figure Legend Snippet: Specificity of M. jannaschii PSTK for the phosphoryl donor. Initial velocities for the phosphotransferase activity of PSTK (6 nM PSTK without NTP or the addition of ATP, ITP, GTP, CTP, UTP or dATP or 200 nM PSTK with AMP-CPP or AMP-PCP) in the presence of the indicated phosphoryl donors (20 mM). Reactions proceeded for 6 min at 37°C and 1.5 min time points were taken and analyzed as in Figure 1 A and 2 . *ND, no detectable phosphorylation. Error bars represent the standard deviation of three experiments.

    Techniques Used: Activity Assay, Conditioned Place Preference, Standard Deviation

    Related Articles

    Polymerase Chain Reaction:

    Article Title: High-Throughput Protein Expression Using a Combination of Ligation-Independent Cloning (LIC) and Infrared Fluorescent Protein (IFP) Detection
    Article Snippet: .. PCR products were treated at 22°C for 30 min with T4 DNA polymerase in the presence of dTTP, using the following reaction setup: 0.2 pmol purified PCR product, 2 µL 10× buffer 2 (NEB), 2 µL dATP (25 mM), 1 µL DTT (100 mM), 2 µL 10× BSA (10 mg/mL; NEB), 1 U T4 DNA polymerase (NEB) in a volume of 20 µL (filled up with ddH2 O). .. The reaction mix was heat inactivated for 20 min at 75°C, followed by purification using the NucleoSpin Extract II kit (Macherey & Nagel) and eluting with 20 µL elution buffer included in the kit.

    Concentration Assay:

    Article Title: Using RecA Protein to Enhance Kinetic Rates of DNA Circuits †
    Article Snippet: .. First, a 10 nM concentration of C30 was annealed with 1 uM RecA ( , solid box) in the presence 2 mM dATP and RecA buffer (70 mM Tris-HCl, 10 mM MgCl2 , 5 mM DTT, pH 7.6), a buffer from New England Biolabs designed for RecA-DNA annealing reactions. ..

    other:

    Article Title: Cell death regulation during influenza A virus infection by matrix (M1) protein: a model of viral control over the cellular survival pathway
    Article Snippet: ATP (NEB, P0756S) or dATP (NEB, N0440S) was used at 1 mM/ml.

    Article Title: Engineering human PrimPol into an efficient RNA-dependent-DNA primase/polymerase
    Article Snippet: Dpn I Restriction enzyme, polynucleotide kinase (PNK), bovine serum albumin (BSA) and 100 mM stock solutions of dATP, dTTP, dGTP and dCTP were purchased from New England Biolabs.

    Plasmid Preparation:

    Article Title: High-Throughput Protein Expression Using a Combination of Ligation-Independent Cloning (LIC) and Infrared Fluorescent Protein (IFP) Detection
    Article Snippet: .. To generate 5′ LIC overhangs (15 and 16 nt, respectively) at both ends the purified vector backbone was treated for 30 min (22°C) with T4 DNA polymerase in the presence of dATP, using the following reaction setup: 0.2 pmol purified vector backbone, 2 µL 10× buffer 2 (NEB), 2 µL dATP (25 mM), 1 µL dithiothreitol (DTT, 100 mM), 2 µL 10× (10 mg/mL) bovine serum albumin (BSA; NEB), 10 U T4 DNA polymerase (NEB) in a volume of 20 µL (filled up with ddH2 O). .. The reaction mix was heat inactivated for 20 min at 75°C, followed by purification using the NucleoSpin Extract II kit (Macherey & Nagel) and elution with 20 µL elution buffer included in the kit.

    Purification:

    Article Title: High-Throughput Protein Expression Using a Combination of Ligation-Independent Cloning (LIC) and Infrared Fluorescent Protein (IFP) Detection
    Article Snippet: .. To generate 5′ LIC overhangs (15 and 16 nt, respectively) at both ends the purified vector backbone was treated for 30 min (22°C) with T4 DNA polymerase in the presence of dATP, using the following reaction setup: 0.2 pmol purified vector backbone, 2 µL 10× buffer 2 (NEB), 2 µL dATP (25 mM), 1 µL dithiothreitol (DTT, 100 mM), 2 µL 10× (10 mg/mL) bovine serum albumin (BSA; NEB), 10 U T4 DNA polymerase (NEB) in a volume of 20 µL (filled up with ddH2 O). .. The reaction mix was heat inactivated for 20 min at 75°C, followed by purification using the NucleoSpin Extract II kit (Macherey & Nagel) and elution with 20 µL elution buffer included in the kit.

    Article Title: Ebolavirus polymerase uses an unconventional genome replication mechanism
    Article Snippet: .. Following purification, the cDNA was tailed with dCTP or dATP using terminal transferase (NEB). .. The tailed cDNA was PCR amplified using a nested virus-specific primer and a primer that annealed specifically with the dATP or the dCTP tail.

    Article Title: High-Throughput Protein Expression Using a Combination of Ligation-Independent Cloning (LIC) and Infrared Fluorescent Protein (IFP) Detection
    Article Snippet: .. PCR products were treated at 22°C for 30 min with T4 DNA polymerase in the presence of dTTP, using the following reaction setup: 0.2 pmol purified PCR product, 2 µL 10× buffer 2 (NEB), 2 µL dATP (25 mM), 1 µL DTT (100 mM), 2 µL 10× BSA (10 mg/mL; NEB), 1 U T4 DNA polymerase (NEB) in a volume of 20 µL (filled up with ddH2 O). .. The reaction mix was heat inactivated for 20 min at 75°C, followed by purification using the NucleoSpin Extract II kit (Macherey & Nagel) and eluting with 20 µL elution buffer included in the kit.