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New England Biolabs itp
Itp, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/itp/product/New England Biolabs
Average 90 stars, based on 3 article reviews
Price from $9.99 to $1999.99
itp - by Bioz Stars, 2019-10
90/100 stars

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In Vitro:

Article Title: Inosine induces context-dependent recoding and translational stalling
Article Snippet: Reporter plasmids were linearized using BglII or EcoRV in case of the plasmid coding for the AGA-codon. .. In vitro transcription was done with NEB HiScribe™ T7 High Yield RNA Synthesis Kit (New England Biolabs, Ipswich, MA) according to the manufacturer's instructions for capped RNA synthesis with the following modifications: synthesis was carried out for 16 h overnight in a 37°C incubator to prevent evaporation using 10 mM of ITP instead of GTP and 2 mM of m7 G cap analog (NEB #S1404S) followed by DNaseI digestion (Thermo Fisher Scientific, MA, Waltham, #EN0521) using 5 units. .. 400 ng of transcript was used for in vitro translation using rabbit reticulocyte lysate (RRL) treated with micrococcal nuclease (Promega, Fitchburg, WI #L4960).

Magnetic Beads:

Article Title: Inosine induces context-dependent recoding and translational stalling
Article Snippet: In vitro transcription was done with NEB HiScribe™ T7 High Yield RNA Synthesis Kit (New England Biolabs, Ipswich, MA) according to the manufacturer's instructions for capped RNA synthesis with the following modifications: synthesis was carried out for 16 h overnight in a 37°C incubator to prevent evaporation using 10 mM of ITP instead of GTP and 2 mM of m7 G cap analog (NEB #S1404S) followed by DNaseI digestion (Thermo Fisher Scientific, MA, Waltham, #EN0521) using 5 units. .. Reactions were carried out in a 100 μl total volume containing 70 μl RRL, 10 mM creatine phosphate, 50 μg/ml creatine phosphokinase, 50 μg/ml calf liver tRNA, 79 mM potassium acetate, 0.5 mM magnesium acetate, 0.02 mM hemin, and a complete mix of amino acids (minus Met mixed with minus Leu) at a concentration of 1 mM.

Evaporation:

Article Title: Inosine induces context-dependent recoding and translational stalling
Article Snippet: Reporter plasmids were linearized using BglII or EcoRV in case of the plasmid coding for the AGA-codon. .. In vitro transcription was done with NEB HiScribe™ T7 High Yield RNA Synthesis Kit (New England Biolabs, Ipswich, MA) according to the manufacturer's instructions for capped RNA synthesis with the following modifications: synthesis was carried out for 16 h overnight in a 37°C incubator to prevent evaporation using 10 mM of ITP instead of GTP and 2 mM of m7 G cap analog (NEB #S1404S) followed by DNaseI digestion (Thermo Fisher Scientific, MA, Waltham, #EN0521) using 5 units. .. 400 ng of transcript was used for in vitro translation using rabbit reticulocyte lysate (RRL) treated with micrococcal nuclease (Promega, Fitchburg, WI #L4960).

Concentration Assay:

Article Title: Inosine induces context-dependent recoding and translational stalling
Article Snippet: In vitro transcription was done with NEB HiScribe™ T7 High Yield RNA Synthesis Kit (New England Biolabs, Ipswich, MA) according to the manufacturer's instructions for capped RNA synthesis with the following modifications: synthesis was carried out for 16 h overnight in a 37°C incubator to prevent evaporation using 10 mM of ITP instead of GTP and 2 mM of m7 G cap analog (NEB #S1404S) followed by DNaseI digestion (Thermo Fisher Scientific, MA, Waltham, #EN0521) using 5 units. .. 400 ng of transcript was used for in vitro translation using rabbit reticulocyte lysate (RRL) treated with micrococcal nuclease (Promega, Fitchburg, WI #L4960).

Incubation:

Article Title: Inosine induces context-dependent recoding and translational stalling
Article Snippet: In vitro transcription was done with NEB HiScribe™ T7 High Yield RNA Synthesis Kit (New England Biolabs, Ipswich, MA) according to the manufacturer's instructions for capped RNA synthesis with the following modifications: synthesis was carried out for 16 h overnight in a 37°C incubator to prevent evaporation using 10 mM of ITP instead of GTP and 2 mM of m7 G cap analog (NEB #S1404S) followed by DNaseI digestion (Thermo Fisher Scientific, MA, Waltham, #EN0521) using 5 units. .. Reactions were carried out in a 100 μl total volume containing 70 μl RRL, 10 mM creatine phosphate, 50 μg/ml creatine phosphokinase, 50 μg/ml calf liver tRNA, 79 mM potassium acetate, 0.5 mM magnesium acetate, 0.02 mM hemin, and a complete mix of amino acids (minus Met mixed with minus Leu) at a concentration of 1 mM.

Plasmid Preparation:

Article Title: Inosine induces context-dependent recoding and translational stalling
Article Snippet: Reporter plasmids were linearized using BglII or EcoRV in case of the plasmid coding for the AGA-codon. .. In vitro transcription was done with NEB HiScribe™ T7 High Yield RNA Synthesis Kit (New England Biolabs, Ipswich, MA) according to the manufacturer's instructions for capped RNA synthesis with the following modifications: synthesis was carried out for 16 h overnight in a 37°C incubator to prevent evaporation using 10 mM of ITP instead of GTP and 2 mM of m7 G cap analog (NEB #S1404S) followed by DNaseI digestion (Thermo Fisher Scientific, MA, Waltham, #EN0521) using 5 units.

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    New England Biolabs itp
    Itp, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/itp/product/New England Biolabs
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    itp - by Bioz Stars, 2019-10
    90/100 stars
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