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Bio-Rad itaq dna polymerase
Itaq Dna Polymerase, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/itaq dna polymerase/product/Bio-Rad
Average 93 stars, based on 85 article reviews
Price from $9.99 to $1999.99
itaq dna polymerase - by Bioz Stars, 2020-04
93/100 stars

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Electrophoresis:

Article Title: Novel spa and Multi-Locus Sequence Types (MLST) of Staphylococcus Aureus Samples Isolated from Clinical Specimens in Korean
Article Snippet: The following reaction mixture was added to each sample: 10 pmol of each primer, 2 μL DNA (100 ng), and 10 μL iQTM cyber (SYBR)® Green supermix (2× reaction buffer with dNTP(dATP, dCTP,dTTP, dGTP)s, iTaq DNA polymerase, SYBR® Green I, fluorescein, and stabilizers; Bio-Rad, Hercules, CA, USA). .. The PCR conditions in the thermal cycler (TC-512, UK) were as follows: 94 °C for 3 min, followed by 35 cycles of denaturation at 94 °C for 30 s, annealing at 56 °C for 30 s, and extension at 72 °C for 45 s. The reaction was ended with a final extension step at 72 °C for 10 min. PCR products were subjected to electrophoresis using 2% agarose gel in 1× TBE buffer at 100 V for 25 min.

Multiplex Assay:

Article Title: Novel spa and Multi-Locus Sequence Types (MLST) of Staphylococcus Aureus Samples Isolated from Clinical Specimens in Korean
Article Snippet: Paragraph title: 4.4. Identification of MecA, BlaTEM, and the Detection of Genes Associated with Antimicrobial Resistance by Multiplex PCR ... The following reaction mixture was added to each sample: 10 pmol of each primer, 2 μL DNA (100 ng), and 10 μL iQTM cyber (SYBR)® Green supermix (2× reaction buffer with dNTP(dATP, dCTP,dTTP, dGTP)s, iTaq DNA polymerase, SYBR® Green I, fluorescein, and stabilizers; Bio-Rad, Hercules, CA, USA).

Agarose Gel Electrophoresis:

Article Title: Novel spa and Multi-Locus Sequence Types (MLST) of Staphylococcus Aureus Samples Isolated from Clinical Specimens in Korean
Article Snippet: The following reaction mixture was added to each sample: 10 pmol of each primer, 2 μL DNA (100 ng), and 10 μL iQTM cyber (SYBR)® Green supermix (2× reaction buffer with dNTP(dATP, dCTP,dTTP, dGTP)s, iTaq DNA polymerase, SYBR® Green I, fluorescein, and stabilizers; Bio-Rad, Hercules, CA, USA). .. The PCR conditions in the thermal cycler (TC-512, UK) were as follows: 94 °C for 3 min, followed by 35 cycles of denaturation at 94 °C for 30 s, annealing at 56 °C for 30 s, and extension at 72 °C for 45 s. The reaction was ended with a final extension step at 72 °C for 10 min. PCR products were subjected to electrophoresis using 2% agarose gel in 1× TBE buffer at 100 V for 25 min.

Synthesized:

Article Title: Gustatory Expression Pattern of the Human TAS2R Bitter Receptor Gene Family Reveals a Heterogenous Population of Bitter Responsive Taste Receptor Cells
Article Snippet: For quantification analyses, the housekeeping gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH), for which primers and probe were designed and synthesized by the Applied Biosystem (Foster City, CA) TaqMan Gene Expression Assay customer service, served as internal control. .. For the PCR reaction, 100 ng of cDNA template was incubated with iTaq Supermix with ROX containing dNTPs with dUTP and iTaq DNA polymerase (P/N 170-8854; Bio-Rad, Hercules, CA) in a final volume of 25 μl.

Quantitative RT-PCR:

Article Title: Tumor-Targeted Dendrimer Nanoparticles for Combinatorial Delivery of siRNA and Chemotherapy for Cancer Treatment.
Article Snippet: In current cancer therapy, the combined targeted delivery of treatments is an important method to enhance the therapeutic efficiency and reduce adverse side effects. .. In current cancer therapy, the combined targeted delivery of treatments is an important method to enhance the therapeutic efficiency and reduce adverse side effects.

Article Title: Gustatory Expression Pattern of the Human TAS2R Bitter Receptor Gene Family Reveals a Heterogenous Population of Bitter Responsive Taste Receptor Cells
Article Snippet: Paragraph title: Quantitative RT-PCR. ... For the PCR reaction, 100 ng of cDNA template was incubated with iTaq Supermix with ROX containing dNTPs with dUTP and iTaq DNA polymerase (P/N 170-8854; Bio-Rad, Hercules, CA) in a final volume of 25 μl.

SYBR Green Assay:

Article Title: Novel spa and Multi-Locus Sequence Types (MLST) of Staphylococcus Aureus Samples Isolated from Clinical Specimens in Korean
Article Snippet: .. The following reaction mixture was added to each sample: 10 pmol of each primer, 2 μL DNA (100 ng), and 10 μL iQTM cyber (SYBR)® Green supermix (2× reaction buffer with dNTP(dATP, dCTP,dTTP, dGTP)s, iTaq DNA polymerase, SYBR® Green I, fluorescein, and stabilizers; Bio-Rad, Hercules, CA, USA). ..

Concentration Assay:

Article Title: Gustatory Expression Pattern of the Human TAS2R Bitter Receptor Gene Family Reveals a Heterogenous Population of Bitter Responsive Taste Receptor Cells
Article Snippet: For the PCR reaction, 100 ng of cDNA template was incubated with iTaq Supermix with ROX containing dNTPs with dUTP and iTaq DNA polymerase (P/N 170-8854; Bio-Rad, Hercules, CA) in a final volume of 25 μl. .. For the PCR reaction, 100 ng of cDNA template was incubated with iTaq Supermix with ROX containing dNTPs with dUTP and iTaq DNA polymerase (P/N 170-8854; Bio-Rad, Hercules, CA) in a final volume of 25 μl.

Incubation:

Article Title: Gustatory Expression Pattern of the Human TAS2R Bitter Receptor Gene Family Reveals a Heterogenous Population of Bitter Responsive Taste Receptor Cells
Article Snippet: .. For the PCR reaction, 100 ng of cDNA template was incubated with iTaq Supermix with ROX containing dNTPs with dUTP and iTaq DNA polymerase (P/N 170-8854; Bio-Rad, Hercules, CA) in a final volume of 25 μl. ..

Polymerase Chain Reaction:

Article Title: Novel spa and Multi-Locus Sequence Types (MLST) of Staphylococcus Aureus Samples Isolated from Clinical Specimens in Korean
Article Snippet: Paragraph title: 4.4. Identification of MecA, BlaTEM, and the Detection of Genes Associated with Antimicrobial Resistance by Multiplex PCR ... The following reaction mixture was added to each sample: 10 pmol of each primer, 2 μL DNA (100 ng), and 10 μL iQTM cyber (SYBR)® Green supermix (2× reaction buffer with dNTP(dATP, dCTP,dTTP, dGTP)s, iTaq DNA polymerase, SYBR® Green I, fluorescein, and stabilizers; Bio-Rad, Hercules, CA, USA).

Article Title: Gustatory Expression Pattern of the Human TAS2R Bitter Receptor Gene Family Reveals a Heterogenous Population of Bitter Responsive Taste Receptor Cells
Article Snippet: .. For the PCR reaction, 100 ng of cDNA template was incubated with iTaq Supermix with ROX containing dNTPs with dUTP and iTaq DNA polymerase (P/N 170-8854; Bio-Rad, Hercules, CA) in a final volume of 25 μl. ..

Expressing:

Article Title: Gustatory Expression Pattern of the Human TAS2R Bitter Receptor Gene Family Reveals a Heterogenous Population of Bitter Responsive Taste Receptor Cells
Article Snippet: For quantification analyses, the housekeeping gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH), for which primers and probe were designed and synthesized by the Applied Biosystem (Foster City, CA) TaqMan Gene Expression Assay customer service, served as internal control. .. For the PCR reaction, 100 ng of cDNA template was incubated with iTaq Supermix with ROX containing dNTPs with dUTP and iTaq DNA polymerase (P/N 170-8854; Bio-Rad, Hercules, CA) in a final volume of 25 μl.

Marker:

Article Title: Novel spa and Multi-Locus Sequence Types (MLST) of Staphylococcus Aureus Samples Isolated from Clinical Specimens in Korean
Article Snippet: The following reaction mixture was added to each sample: 10 pmol of each primer, 2 μL DNA (100 ng), and 10 μL iQTM cyber (SYBR)® Green supermix (2× reaction buffer with dNTP(dATP, dCTP,dTTP, dGTP)s, iTaq DNA polymerase, SYBR® Green I, fluorescein, and stabilizers; Bio-Rad, Hercules, CA, USA). .. The 100 bp DNA ladder (Bioneer, Daejeon, Korea) was used as a molecular size marker.

Real-time Polymerase Chain Reaction:

Article Title: Tumor-Targeted Dendrimer Nanoparticles for Combinatorial Delivery of siRNA and Chemotherapy for Cancer Treatment.
Article Snippet: In current cancer therapy, the combined targeted delivery of treatments is an important method to enhance the therapeutic efficiency and reduce adverse side effects. .. In current cancer therapy, the combined targeted delivery of treatments is an important method to enhance the therapeutic efficiency and reduce adverse side effects.

Article Title: Gustatory Expression Pattern of the Human TAS2R Bitter Receptor Gene Family Reveals a Heterogenous Population of Bitter Responsive Taste Receptor Cells
Article Snippet: Quantitative real-time PCR analysis was performed on a TaqMan ABI Prism 7300 Real Time PCR System (Applied Biosystems, Darmstadt, Germany). .. For the PCR reaction, 100 ng of cDNA template was incubated with iTaq Supermix with ROX containing dNTPs with dUTP and iTaq DNA polymerase (P/N 170-8854; Bio-Rad, Hercules, CA) in a final volume of 25 μl.

Transmission Electron Microscopy:

Article Title: Novel spa and Multi-Locus Sequence Types (MLST) of Staphylococcus Aureus Samples Isolated from Clinical Specimens in Korean
Article Snippet: Identification of MecA, BlaTEM, and the Detection of Genes Associated with Antimicrobial Resistance by Multiplex PCR The PCR primers used to detect mecA , bla TEM, and antimicrobial resistance genes are listed in references [ , , ]. .. The following reaction mixture was added to each sample: 10 pmol of each primer, 2 μL DNA (100 ng), and 10 μL iQTM cyber (SYBR)® Green supermix (2× reaction buffer with dNTP(dATP, dCTP,dTTP, dGTP)s, iTaq DNA polymerase, SYBR® Green I, fluorescein, and stabilizers; Bio-Rad, Hercules, CA, USA).

Software:

Article Title: Gustatory Expression Pattern of the Human TAS2R Bitter Receptor Gene Family Reveals a Heterogenous Population of Bitter Responsive Taste Receptor Cells
Article Snippet: For the PCR reaction, 100 ng of cDNA template was incubated with iTaq Supermix with ROX containing dNTPs with dUTP and iTaq DNA polymerase (P/N 170-8854; Bio-Rad, Hercules, CA) in a final volume of 25 μl. .. The open reading frames of all 25 hTAS2Rs were cloned into a vector cassette adding an N-terminal rat somatostatin receptor subtype 3 (sst3) tag and a C-terminally located herpes simplex virus glycoprotein D epitope (HSV) originally described by .

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    Bio-Rad biorad iq supermix
    Biorad Iq Supermix, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Price from $9.99 to $1999.99
    iqtm sybr green supermix buffer - by Bioz Stars, 2020-04
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