isothermal assembly reaction buffer  (New England Biolabs)


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  • 90
    Name:
    Isothermal Amplification Buffer
    Description:
    Isothermal Amplification Buffer 6 0 ml
    Catalog Number:
    b0537s
    Price:
    28
    Size:
    6 0 ml
    Category:
    Buffers
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    Structured Review

    New England Biolabs isothermal assembly reaction buffer
    Isothermal Amplification Buffer
    Isothermal Amplification Buffer 6 0 ml
    https://www.bioz.com/result/isothermal assembly reaction buffer/product/New England Biolabs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    isothermal assembly reaction buffer - by Bioz Stars, 2020-09
    90/100 stars

    Related Products / Commonly Used Together

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    phusion dna polymerase
    taq dna ligase
    dntp

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    Related Articles

    Amplification:

    Article Title: Development of a real-time loop-mediated isothermal amplification assay for detection of porcine circovirus 3
    Article Snippet: .. Development of real-time LAMP assay The real-time LAMP reaction mixture contained template DNA or cDNA, inner primers (FIP and BIP) at 1.6 mM, outer primers (F3 and B3) at 0.2 mM, loop backward primers (LB) at 0.8 mM, 1 μL of Bst DNA polymerase (M5038 L, New England Biolabs, MA, USA), betaine at 0.8 M (BCBS087V, Sigma St. Louis, MO USA), dNTPs at 1.4 mM, 2.5 μL of amplification buffer (B0537S, New England Biolabs), 8 mM of MgSO4 and SYTO-9 green fluorescent nucleic acid stain at 0.1 mM (S34854, Invitrogen, Carlsbad, CA, USA). .. Double-distilled water was used as a negative control.

    Article Title: Development of a loop-mediated isothermal amplification (LAMP) assay for rapid screening of ticks and fleas for spotted fever group rickettsia
    Article Snippet: .. Bst 2.0 LAMP Each LAMP reaction totaled 25 μl consisting of 9 μl nuclease free water, 2.5 μl 10x Isothermal Amplification Buffer Pack (contains 20mM Tris-HCl, 10mM (NH4 )2 SO4 , 50mM KCl, 2mM MgSO4 , 0.1% Tween® 20) (New England Biolabs), 3.5 μl 10 mM each dNTPs (New England Biolabs), 1 μl 100 mM MgSO4 (New England Biolabs), 3.5 μl 5M Betaine (Affymetrix), and 2.5 μl 10x primer mix (2μM F3 primer, 2μM B3 primer, 16μM FIP, 16μM BIP, 8μM LoopF and 8μM LoopB), 1.0 μl Bst 2.0 WarmStart DNA Polymerase (8 units/μl) (New England Biolabs), 1.0 μl 3mM Hydroxynaphthol blue (HNB) (Sigma-Aldrich), and 1 μl of sample DNA. ..

    Article Title: Direct and Rapid Detection and Quantification of Oenococcus oeni Cells in Wine by Cells-LAMP and Cells-qLAMP
    Article Snippet: .. Each reaction contained 1.4 mM of dNTPs, 0.2 μM of each outer primer, 1.6 μM of each inner primer, 0.8 μM of each loop primer, 8 mM of MgCl2 (Invitrogen), 1× Isothermal Amplification Buffer, 0.4 U/μL of Bst polymerase 2.0 (New England BioLabs), 11.5 μL of the mechanically lysed cells, and 0.4 μM of SYTO-9 (Invitrogen) or 1 μL of 10× SYBR Green I (Invitrogen). .. The qLAMP amplifications were performed in triplicate in a C100TM Thermal Cycler, CFX96TM Real-Time System (BioRad), which operated at a constant temperature of 62°C for 1 h. Fluorescence signals were collected every minute, followed by a melting curve analysis obtained by slow heating from 60°C to 95°C at 0.5°C every 5 s, with continuous fluorescence collection.

    Article Title: Sensitive Detection of Xanthomonas oryzae Pathovars oryzae and oryzicola by Loop-Mediated Isothermal Amplification
    Article Snippet: .. The 25-μl reaction mix contained 2.5 μl 10× isothermal amplification buffer (New England BioLabs, Ipswich, MA), 1.4 mM deoxynucleoside triphosphates, 6 mM additional MgSO4 for a final MgSO4 concentration of 8 mM (New England BioLabs, Ipswich, MA), 0.8 M betaine (Sigma-Aldrich, St. Louis, MO), 4 U Bst DNA polymerase large fragment or Bst DNA polymerase 2.0 (New England BioLabs, Ipswich, MA), 0.32 μM primers FIP and BIP, 32 nM primers F3 and B3, and 0.16 μM primers LoopF and LoopB (loop primers in pathovar-specific assays only) with 1 μl of 20 ng μl−1 DNA, heat-killed cells, or plant extract. .. Mineral oil (EMD Millipore, Darmstadt, Germany) was added on top of the reaction mixture (20 μl) to minimize the introduction of aerosolized product into the work spaces.

    Article Title: A field survey using LAMP assay for detection of Schistosoma mansoni in a low-transmission area of schistosomiasis in Umbuzeiro, Brazil: Assessment in human and snail samples
    Article Snippet: .. Briefly, the reaction was carried out in 25 μL reaction mixture containing 40 pmol each of FIP and BIP primers, 5 pmol of each F3 and B3 primers, 1.4 mM of each dNTP (Intron), 1x Isothermal Amplification Buffer -20 mM Tris-HCl (pH 8.8), 50 mM KCl, 10 mM (NH4 )2 SO4 , 2 mM MgSO4 , 0.1% Tween20- (New England Biolabs, UK), 1 M betaine (Sigma, USA), supplementary 6 mM of MgSO4 (New England Biolabs, UK) and 8 U of Bst 2.0 WarmStart DNA polymerase (New England Biolabs, UK) with 2 μL of template DNA. .. Reaction tubes were placed in a heating block at a constant temperature of 63°C for 60 min and then heated at 80°C for 5 min to stop the reaction.

    Article Title: A smartphone-based diagnostic platform for rapid detection of Zika, chikungunya, and dengue viruses
    Article Snippet: .. The fresh reaction mixture contained 1x Isothermal Amplification Buffer (20 mM Tris-HCl, 10 mM (NH4 )2SO4 , 50 mM KCl, 2 mM MgSO4 , 0.1% Tween® 20, pH 8.8@25 °C, NEB), 700 mM of Betaine (Sigma), 0.14 mM of each nucleotide, 8 mM MgSO4 , 2 μM SYTO® 9 Stain (Thermo Fisher Scientific), 2 units of Porcine RNase Inhibitor , 0.2 μM each F3 and B3, 0.8 μM each LF and LB, 1.6 μM each FIP and BIP primers , 3.2 units of avian myeloblastosis virus (AMV) reverse transcriptase (Life Sciences Advanced Technologies Inc., St Petersburg, FL), and 3.2 units of Bst 2.0 WarmStart® DNA Polymerase (NEB). .. To make the dried reagent formulation, isothermal amplification buffer, betaine and magnesium sulfate were removed.

    Article Title: Loop-Mediated Isothermal Amplification (LAMP) for Rapid Detection and Quantification of Dehalococcoides Biomarker Genes in Commercial Reductive Dechlorinating Cultures KB-1 and SDC-9
    Article Snippet: .. Each 20-μl LAMP reaction mixture contained 1× isothermal amplification buffer (catalog no. B0537S; NEB), 1.4 mM deoxynucleoside triphosphates (dNTPs), 0.8 mM betaine, 6.0 mM MgSO4 , 1.6 U of Bst 2.0 WarmStart (NEB), 0.8 μl of Syto82 orange fluorescent dye (Life Technologies, Inc., Grand Island, NY), 0.8 μl of Pluronic (Life Technologies, Inc.), 0.8 μl of bovine serum albumin, a 0.25 μM concentration of 10× primer mix, and balance water to make up 18 μl. ..

    SYBR Green Assay:

    Article Title: Direct and Rapid Detection and Quantification of Oenococcus oeni Cells in Wine by Cells-LAMP and Cells-qLAMP
    Article Snippet: .. Each reaction contained 1.4 mM of dNTPs, 0.2 μM of each outer primer, 1.6 μM of each inner primer, 0.8 μM of each loop primer, 8 mM of MgCl2 (Invitrogen), 1× Isothermal Amplification Buffer, 0.4 U/μL of Bst polymerase 2.0 (New England BioLabs), 11.5 μL of the mechanically lysed cells, and 0.4 μM of SYTO-9 (Invitrogen) or 1 μL of 10× SYBR Green I (Invitrogen). .. The qLAMP amplifications were performed in triplicate in a C100TM Thermal Cycler, CFX96TM Real-Time System (BioRad), which operated at a constant temperature of 62°C for 1 h. Fluorescence signals were collected every minute, followed by a melting curve analysis obtained by slow heating from 60°C to 95°C at 0.5°C every 5 s, with continuous fluorescence collection.

    Concentration Assay:

    Article Title: Sensitive Detection of Xanthomonas oryzae Pathovars oryzae and oryzicola by Loop-Mediated Isothermal Amplification
    Article Snippet: .. The 25-μl reaction mix contained 2.5 μl 10× isothermal amplification buffer (New England BioLabs, Ipswich, MA), 1.4 mM deoxynucleoside triphosphates, 6 mM additional MgSO4 for a final MgSO4 concentration of 8 mM (New England BioLabs, Ipswich, MA), 0.8 M betaine (Sigma-Aldrich, St. Louis, MO), 4 U Bst DNA polymerase large fragment or Bst DNA polymerase 2.0 (New England BioLabs, Ipswich, MA), 0.32 μM primers FIP and BIP, 32 nM primers F3 and B3, and 0.16 μM primers LoopF and LoopB (loop primers in pathovar-specific assays only) with 1 μl of 20 ng μl−1 DNA, heat-killed cells, or plant extract. .. Mineral oil (EMD Millipore, Darmstadt, Germany) was added on top of the reaction mixture (20 μl) to minimize the introduction of aerosolized product into the work spaces.

    Article Title: Loop-Mediated Isothermal Amplification (LAMP) for Rapid Detection and Quantification of Dehalococcoides Biomarker Genes in Commercial Reductive Dechlorinating Cultures KB-1 and SDC-9
    Article Snippet: .. Each 20-μl LAMP reaction mixture contained 1× isothermal amplification buffer (catalog no. B0537S; NEB), 1.4 mM deoxynucleoside triphosphates (dNTPs), 0.8 mM betaine, 6.0 mM MgSO4 , 1.6 U of Bst 2.0 WarmStart (NEB), 0.8 μl of Syto82 orange fluorescent dye (Life Technologies, Inc., Grand Island, NY), 0.8 μl of Pluronic (Life Technologies, Inc.), 0.8 μl of bovine serum albumin, a 0.25 μM concentration of 10× primer mix, and balance water to make up 18 μl. ..

    Lamp Assay:

    Article Title: Development of a real-time loop-mediated isothermal amplification assay for detection of porcine circovirus 3
    Article Snippet: .. Development of real-time LAMP assay The real-time LAMP reaction mixture contained template DNA or cDNA, inner primers (FIP and BIP) at 1.6 mM, outer primers (F3 and B3) at 0.2 mM, loop backward primers (LB) at 0.8 mM, 1 μL of Bst DNA polymerase (M5038 L, New England Biolabs, MA, USA), betaine at 0.8 M (BCBS087V, Sigma St. Louis, MO USA), dNTPs at 1.4 mM, 2.5 μL of amplification buffer (B0537S, New England Biolabs), 8 mM of MgSO4 and SYTO-9 green fluorescent nucleic acid stain at 0.1 mM (S34854, Invitrogen, Carlsbad, CA, USA). .. Double-distilled water was used as a negative control.

    Modification:

    Article Title: A hydrogel beads based platform for single-cell phenotypic analysis and digital molecular detection
    Article Snippet: .. Each 20 μL of modified LAMP mix for digital single bacteria LAMP contained 1× isothermal buffer, 6 mM total MgSO4, 1.4 mM dNTP, 640 U/mL Bst 2.0 WarmStart polymerase, 1.6 μM FIB and BIP, 0.2 μM F3 and B3, 0.8 μM LF and LB, 1.5 mg/mL BSA, 1× LAMP dye ( ). ..

    Staining:

    Article Title: Development of a real-time loop-mediated isothermal amplification assay for detection of porcine circovirus 3
    Article Snippet: .. Development of real-time LAMP assay The real-time LAMP reaction mixture contained template DNA or cDNA, inner primers (FIP and BIP) at 1.6 mM, outer primers (F3 and B3) at 0.2 mM, loop backward primers (LB) at 0.8 mM, 1 μL of Bst DNA polymerase (M5038 L, New England Biolabs, MA, USA), betaine at 0.8 M (BCBS087V, Sigma St. Louis, MO USA), dNTPs at 1.4 mM, 2.5 μL of amplification buffer (B0537S, New England Biolabs), 8 mM of MgSO4 and SYTO-9 green fluorescent nucleic acid stain at 0.1 mM (S34854, Invitrogen, Carlsbad, CA, USA). .. Double-distilled water was used as a negative control.

    Article Title: A smartphone-based diagnostic platform for rapid detection of Zika, chikungunya, and dengue viruses
    Article Snippet: .. The fresh reaction mixture contained 1x Isothermal Amplification Buffer (20 mM Tris-HCl, 10 mM (NH4 )2SO4 , 50 mM KCl, 2 mM MgSO4 , 0.1% Tween® 20, pH 8.8@25 °C, NEB), 700 mM of Betaine (Sigma), 0.14 mM of each nucleotide, 8 mM MgSO4 , 2 μM SYTO® 9 Stain (Thermo Fisher Scientific), 2 units of Porcine RNase Inhibitor , 0.2 μM each F3 and B3, 0.8 μM each LF and LB, 1.6 μM each FIP and BIP primers , 3.2 units of avian myeloblastosis virus (AMV) reverse transcriptase (Life Sciences Advanced Technologies Inc., St Petersburg, FL), and 3.2 units of Bst 2.0 WarmStart® DNA Polymerase (NEB). .. To make the dried reagent formulation, isothermal amplification buffer, betaine and magnesium sulfate were removed.

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  • News
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  • 90
    New England Biolabs isothermal buffer
    Isothermal Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/isothermal buffer/product/New England Biolabs
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    isothermal buffer - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    90
    New England Biolabs isothermal assembly reaction buffer
    Isothermal Assembly Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/isothermal assembly reaction buffer/product/New England Biolabs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    isothermal assembly reaction buffer - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

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