isobutylmethylxanthine ibmx  (Millipore)


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    Structured Review

    Millipore isobutylmethylxanthine ibmx
    Activation of HOXB4 resulted in a modest increase in the proportion of immature CD235a + /CD71 + erythroid cells. HOXB4 was activated with 4OHT from days 10 to 24 (A) . Production of erythroid cells was monitored by expression of CD235a and CD71 by flow cytometry at day 24 (B–D) . Data were generated from four independent experiments with error bars representing the SEM. ∗, p = .05. Abbreviations: 4OHT, tamoxifen; BMP4, bone morphogenetic protein 4; C, cell; CFU, colony-forming unit; EPO, erythropoietin; FGF a, fibroblast growth factor-α; FLT3L, FMS-like tyrosine kinase receptor 3 ligand; GSK, glycogen synthase kinase; <t>IBMX,</t> <t>isobutylmethylxanthine;</t> IGF1, IGF2, insulin-like growth factor 1, 2; IL, interleukin; Q, quartile; SCF, stem cell factor; TPO, thrombopoietin; VEGF, vascular endothelial growth factor.
    Isobutylmethylxanthine Ibmx, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/isobutylmethylxanthine ibmx/product/Millipore
    Average 99 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    isobutylmethylxanthine ibmx - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "Enforced Expression of HOXB4 in Human Embryonic Stem Cells Enhances the Production of Hematopoietic Progenitors but Has No Effect on the Maturation of Red Blood Cells"

    Article Title: Enforced Expression of HOXB4 in Human Embryonic Stem Cells Enhances the Production of Hematopoietic Progenitors but Has No Effect on the Maturation of Red Blood Cells

    Journal: Stem Cells Translational Medicine

    doi: 10.5966/sctm.2015-0324

    Activation of HOXB4 resulted in a modest increase in the proportion of immature CD235a + /CD71 + erythroid cells. HOXB4 was activated with 4OHT from days 10 to 24 (A) . Production of erythroid cells was monitored by expression of CD235a and CD71 by flow cytometry at day 24 (B–D) . Data were generated from four independent experiments with error bars representing the SEM. ∗, p = .05. Abbreviations: 4OHT, tamoxifen; BMP4, bone morphogenetic protein 4; C, cell; CFU, colony-forming unit; EPO, erythropoietin; FGF a, fibroblast growth factor-α; FLT3L, FMS-like tyrosine kinase receptor 3 ligand; GSK, glycogen synthase kinase; IBMX, isobutylmethylxanthine; IGF1, IGF2, insulin-like growth factor 1, 2; IL, interleukin; Q, quartile; SCF, stem cell factor; TPO, thrombopoietin; VEGF, vascular endothelial growth factor.
    Figure Legend Snippet: Activation of HOXB4 resulted in a modest increase in the proportion of immature CD235a + /CD71 + erythroid cells. HOXB4 was activated with 4OHT from days 10 to 24 (A) . Production of erythroid cells was monitored by expression of CD235a and CD71 by flow cytometry at day 24 (B–D) . Data were generated from four independent experiments with error bars representing the SEM. ∗, p = .05. Abbreviations: 4OHT, tamoxifen; BMP4, bone morphogenetic protein 4; C, cell; CFU, colony-forming unit; EPO, erythropoietin; FGF a, fibroblast growth factor-α; FLT3L, FMS-like tyrosine kinase receptor 3 ligand; GSK, glycogen synthase kinase; IBMX, isobutylmethylxanthine; IGF1, IGF2, insulin-like growth factor 1, 2; IL, interleukin; Q, quartile; SCF, stem cell factor; TPO, thrombopoietin; VEGF, vascular endothelial growth factor.

    Techniques Used: Activation Assay, Expressing, Flow Cytometry, Cytometry, Generated

    Activation of HOXB4 enhanced the production of hematopoietic progenitors. Tamoxifen (labeled + or −) was added to cultures to activate HOXB4 in H1 human embryonic stem cells (hESCs) expressing HOXB4-ER T2 (labeled HOXB4) or parental H1 hESCs (labeled control) between days 0 and 10 (A) and then assessed for CFU-C activity (B) and expression of hematopoietic progenitor markers, CD34 and CD43, by flow cytometry (C, D) . Data were generated from four independent experiments, with error bars representing SEM. ∗, p = .03; ∗∗, p = .04. Abbreviations: 4OHT, tamoxifen; BFU-E, burst-forming unit erythroid; BMP4, bone morphogenetic protein 4; C, cell; CFU, colony-forming unit; FGF a, fibroblast growth factor-α; GM, granulocyte macrophage; GSK, glycogen synthase kinase; IBMX, isobutylmethylxanthine; IGF2, insulin-like growth factor 2; M, macrophage; Q, quartile; SCF, stem cell factor; TPO, thrombopoietin; VEGF, vascular endothelial growth factor.
    Figure Legend Snippet: Activation of HOXB4 enhanced the production of hematopoietic progenitors. Tamoxifen (labeled + or −) was added to cultures to activate HOXB4 in H1 human embryonic stem cells (hESCs) expressing HOXB4-ER T2 (labeled HOXB4) or parental H1 hESCs (labeled control) between days 0 and 10 (A) and then assessed for CFU-C activity (B) and expression of hematopoietic progenitor markers, CD34 and CD43, by flow cytometry (C, D) . Data were generated from four independent experiments, with error bars representing SEM. ∗, p = .03; ∗∗, p = .04. Abbreviations: 4OHT, tamoxifen; BFU-E, burst-forming unit erythroid; BMP4, bone morphogenetic protein 4; C, cell; CFU, colony-forming unit; FGF a, fibroblast growth factor-α; GM, granulocyte macrophage; GSK, glycogen synthase kinase; IBMX, isobutylmethylxanthine; IGF2, insulin-like growth factor 2; M, macrophage; Q, quartile; SCF, stem cell factor; TPO, thrombopoietin; VEGF, vascular endothelial growth factor.

    Techniques Used: Activation Assay, Labeling, Expressing, Activity Assay, Flow Cytometry, Cytometry, Generated

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    Staining:

    Article Title: Interaction between von Hippel-Lindau Protein and Fatty Acid Synthase Modulates Hypoxia Target Gene Expression
    Article Snippet: .. 3T3-L1 cells (ATCC, CL-173) were cultured in DMEM and differentiated with insulin, dexamethasone, and 3-isobutyl-1-methylxanthine (Sigma-Aldrich, I-6634, D-4902, and I-5879 respectively), and stained with Oil Red O (Sigma-Aldrich, O-0625) as previously reported . .. MG132 (C-2211) and 25-hydroxycholesterol (H1015) were purchased from Sigma-Aldrich.

    Incubation:

    Article Title: Prolyl Isomerase Pin1 Regulates Mouse Embryonic Fibroblast Differentiation into Adipose Cells
    Article Snippet: .. Differentiation of Cells NIH3T3-L1 (control and Pin1-knockdown) and MEFs (wild-type and Pin1−/− ) were incubated in DMEM medium, containing 0.5 mM 3-isobutyl- 1-methylxanthine (Nakalai), 1 µM dexamethasone (Sigma) and 1.7 µM insulin (Wako) for 48 hours. .. Cells were cultured in insulin-containing medium for 48 hours.

    Recombinant:

    Article Title: Isolation of alveolar epithelial type II progenitor cells from adult human lungs
    Article Snippet: .. After 4 h, the media was changed to DMEM containing 5% FBS with 10 ng/ml human recombinant keratinocyte growth factor (KGF; PeproTech, Rocky Hill, NJ, USA), 0.1 mM 8-bromo cAMP (cAMP; Sigma-Aldrich) and 0.1 mM IBMX (Sigma-Aldrich). .. For hematoxylin–eosin staining, whole cell-embedded gels were fixed with 10% formalin and embedded with paraffin.

    other:

    Article Title: Intracellular potentiation between two second messenger systems may contribute to cholera toxin induced intestinal secretion in humans
    Article Snippet: ACh chloride, barium chloride, cAMP, dimethyl sulphoxide, forskolin, histamine dihydrochloride, hyoscine hydrobromide, IBMX, charybdotoxin, apamin, and vasoactive intestinal peptide were all obtained from Sigma (UK).

    Cell Culture:

    Article Title: Interaction between von Hippel-Lindau Protein and Fatty Acid Synthase Modulates Hypoxia Target Gene Expression
    Article Snippet: .. 3T3-L1 cells (ATCC, CL-173) were cultured in DMEM and differentiated with insulin, dexamethasone, and 3-isobutyl-1-methylxanthine (Sigma-Aldrich, I-6634, D-4902, and I-5879 respectively), and stained with Oil Red O (Sigma-Aldrich, O-0625) as previously reported . .. MG132 (C-2211) and 25-hydroxycholesterol (H1015) were purchased from Sigma-Aldrich.

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