isoamyl alcohol rnasin plus rnase inhibitor  (Promega)

 
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    Structured Review

    Promega isoamyl alcohol rnasin plus rnase inhibitor
    Isoamyl Alcohol Rnasin Plus Rnase Inhibitor, supplied by Promega, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/isoamyl alcohol rnasin plus rnase inhibitor/product/Promega
    Average 78 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    isoamyl alcohol rnasin plus rnase inhibitor - by Bioz Stars, 2020-03
    78/100 stars

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    Polymerase Chain Reaction:

    Article Title: Genetic Manipulation of Stenotrophomonas maltophilia
    Article Snippet: Note: Use 2 to 5 μl of supernatant per PCR reaction. .. Viable S. maltophilia on a petri plate (see UNIT 6F.1 ) Luria Bertani (LB) Broth (APPENDIX 4A) Acid phenol:chloroform (pH 4.3 to 4.7) Chloroform ddH2 O, nuclease free 70% ethanol in nuclease-free ddH2 O, ice-cold 100% (absolute) ethanol, ice-cold Isoamyl alcohol RNasin® Plus RNase Inhibitor (Promega) Dithiothreitol (DTT), 0.1 M RQ1 RNase-Free DNase RQ1 RNase-Free DNase buffer, 10× Sample Buffer (see recipe) Stop Solution (see recipe) Sodium acetate, 3 M pH 5.2 Sodium dodecyl sulfate (SDS), 20% TE buffer, pH 8.0 (APPENDIX 2A) Bead beating tubes, 8 ml 15 ml and 50 ml conical tube, sterile Acid-washed zirconium beads, 0.1 mm (see recipe) Beadbeater (Biospec Products) Microcentrifuge tubes, sterile Swinging bucket centrifuge, pre-chilled to 4° C Orbital shaker set to 37° C, 225 rpm Water bath set to 37° C Water bath set to 65° C Ice bath Inoculate a single isolated colony of S. maltophilia into 10 ml LB broth and incubate at 37° C until stationary phase is reached, shaking at 225 rpm ( UNIT 6F.1 ).

    Isolation:

    Article Title: Genetic Manipulation of Stenotrophomonas maltophilia
    Article Snippet: .. Viable S. maltophilia on a petri plate (see UNIT 6F.1 ) Luria Bertani (LB) Broth (APPENDIX 4A) Acid phenol:chloroform (pH 4.3 to 4.7) Chloroform ddH2 O, nuclease free 70% ethanol in nuclease-free ddH2 O, ice-cold 100% (absolute) ethanol, ice-cold Isoamyl alcohol RNasin® Plus RNase Inhibitor (Promega) Dithiothreitol (DTT), 0.1 M RQ1 RNase-Free DNase RQ1 RNase-Free DNase buffer, 10× Sample Buffer (see recipe) Stop Solution (see recipe) Sodium acetate, 3 M pH 5.2 Sodium dodecyl sulfate (SDS), 20% TE buffer, pH 8.0 (APPENDIX 2A) Bead beating tubes, 8 ml 15 ml and 50 ml conical tube, sterile Acid-washed zirconium beads, 0.1 mm (see recipe) Beadbeater (Biospec Products) Microcentrifuge tubes, sterile Swinging bucket centrifuge, pre-chilled to 4° C Orbital shaker set to 37° C, 225 rpm Water bath set to 37° C Water bath set to 65° C Ice bath Inoculate a single isolated colony of S. maltophilia into 10 ml LB broth and incubate at 37° C until stationary phase is reached, shaking at 225 rpm ( UNIT 6F.1 ). .. Add 1 ml Stop Solution to the culture and briefly vortex for 10 seconds.

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  • 78
    Promega isoamyl alcohol rnasin plus rnase inhibitor
    Isoamyl Alcohol Rnasin Plus Rnase Inhibitor, supplied by Promega, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/isoamyl alcohol rnasin plus rnase inhibitor/product/Promega
    Average 78 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    isoamyl alcohol rnasin plus rnase inhibitor - by Bioz Stars, 2020-03
    78/100 stars
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