islet1  (Abcam)

 
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    Name:
    Anti Islet 1 antibody 1B1
    Description:

    Catalog Number:
    ab86501
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    Structured Review

    Abcam islet1
    Characterization of NCPCs with specific markers. On day 10, cells presented neural progenitor cell’s morphology and positive staining for Nestin (A,D,G,J,M) , TRPV1 (B) , Peripherin (E) , BRN3A (H) , Pax6 (K) , and negative for <t>Islet1</t> (N) . Nuclei were stained with DAPI and superimposed with staining for nestin and TRPV1 (C) , Peripherin (F) , BRN3A (I) , Pax6 (L) , and Islet1 (O) . Calibration bar = 100 μm. Quantification of positive cells for each marker relative to DAPI-stained nuclei, n = 2–3 independent experiments (P) .

    https://www.bioz.com/result/islet1/product/Abcam
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    islet1 - by Bioz Stars, 2020-07
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    Images

    1) Product Images from "Generation of iPSC-Derived Human Peripheral Sensory Neurons Releasing Substance P Elicited by TRPV1 Agonists"

    Article Title: Generation of iPSC-Derived Human Peripheral Sensory Neurons Releasing Substance P Elicited by TRPV1 Agonists

    Journal: Frontiers in Molecular Neuroscience

    doi: 10.3389/fnmol.2018.00277

    Characterization of NCPCs with specific markers. On day 10, cells presented neural progenitor cell’s morphology and positive staining for Nestin (A,D,G,J,M) , TRPV1 (B) , Peripherin (E) , BRN3A (H) , Pax6 (K) , and negative for Islet1 (N) . Nuclei were stained with DAPI and superimposed with staining for nestin and TRPV1 (C) , Peripherin (F) , BRN3A (I) , Pax6 (L) , and Islet1 (O) . Calibration bar = 100 μm. Quantification of positive cells for each marker relative to DAPI-stained nuclei, n = 2–3 independent experiments (P) .
    Figure Legend Snippet: Characterization of NCPCs with specific markers. On day 10, cells presented neural progenitor cell’s morphology and positive staining for Nestin (A,D,G,J,M) , TRPV1 (B) , Peripherin (E) , BRN3A (H) , Pax6 (K) , and negative for Islet1 (N) . Nuclei were stained with DAPI and superimposed with staining for nestin and TRPV1 (C) , Peripherin (F) , BRN3A (I) , Pax6 (L) , and Islet1 (O) . Calibration bar = 100 μm. Quantification of positive cells for each marker relative to DAPI-stained nuclei, n = 2–3 independent experiments (P) .

    Techniques Used: Staining, Marker

    2) Product Images from "Generation of iPSC-Derived Human Peripheral Sensory Neurons Releasing Substance P Elicited by TRPV1 Agonists"

    Article Title: Generation of iPSC-Derived Human Peripheral Sensory Neurons Releasing Substance P Elicited by TRPV1 Agonists

    Journal: Frontiers in Molecular Neuroscience

    doi: 10.3389/fnmol.2018.00277

    Characterization of NCPCs with specific markers. On day 10, cells presented neural progenitor cell’s morphology and positive staining for Nestin (A,D,G,J,M) , TRPV1 (B) , Peripherin (E) , BRN3A (H) , Pax6 (K) , and negative for Islet1 (N) . Nuclei were stained with DAPI and superimposed with staining for nestin and TRPV1 (C) , Peripherin (F) , BRN3A (I) , Pax6 (L) , and Islet1 (O) . Calibration bar = 100 μm. Quantification of positive cells for each marker relative to DAPI-stained nuclei, n = 2–3 independent experiments (P) .
    Figure Legend Snippet: Characterization of NCPCs with specific markers. On day 10, cells presented neural progenitor cell’s morphology and positive staining for Nestin (A,D,G,J,M) , TRPV1 (B) , Peripherin (E) , BRN3A (H) , Pax6 (K) , and negative for Islet1 (N) . Nuclei were stained with DAPI and superimposed with staining for nestin and TRPV1 (C) , Peripherin (F) , BRN3A (I) , Pax6 (L) , and Islet1 (O) . Calibration bar = 100 μm. Quantification of positive cells for each marker relative to DAPI-stained nuclei, n = 2–3 independent experiments (P) .

    Techniques Used: Staining, Marker

    3) Product Images from "Generation of iPSC-Derived Human Peripheral Sensory Neurons Releasing Substance P Elicited by TRPV1 Agonists"

    Article Title: Generation of iPSC-Derived Human Peripheral Sensory Neurons Releasing Substance P Elicited by TRPV1 Agonists

    Journal: Frontiers in Molecular Neuroscience

    doi: 10.3389/fnmol.2018.00277

    Characterization of NCPCs with specific markers. On day 10, cells presented neural progenitor cell’s morphology and positive staining for Nestin (A,D,G,J,M) , TRPV1 (B) , Peripherin (E) , BRN3A (H) , Pax6 (K) , and negative for Islet1 (N) . Nuclei were stained with DAPI and superimposed with staining for nestin and TRPV1 (C) , Peripherin (F) , BRN3A (I) , Pax6 (L) , and Islet1 (O) . Calibration bar = 100 μm. Quantification of positive cells for each marker relative to DAPI-stained nuclei, n = 2–3 independent experiments (P) .
    Figure Legend Snippet: Characterization of NCPCs with specific markers. On day 10, cells presented neural progenitor cell’s morphology and positive staining for Nestin (A,D,G,J,M) , TRPV1 (B) , Peripherin (E) , BRN3A (H) , Pax6 (K) , and negative for Islet1 (N) . Nuclei were stained with DAPI and superimposed with staining for nestin and TRPV1 (C) , Peripherin (F) , BRN3A (I) , Pax6 (L) , and Islet1 (O) . Calibration bar = 100 μm. Quantification of positive cells for each marker relative to DAPI-stained nuclei, n = 2–3 independent experiments (P) .

    Techniques Used: Staining, Marker

    4) Product Images from "Generation of iPSC-Derived Human Peripheral Sensory Neurons Releasing Substance P Elicited by TRPV1 Agonists"

    Article Title: Generation of iPSC-Derived Human Peripheral Sensory Neurons Releasing Substance P Elicited by TRPV1 Agonists

    Journal: Frontiers in Molecular Neuroscience

    doi: 10.3389/fnmol.2018.00277

    Characterization of NCPCs with specific markers. On day 10, cells presented neural progenitor cell’s morphology and positive staining for Nestin (A,D,G,J,M) , TRPV1 (B) , Peripherin (E) , BRN3A (H) , Pax6 (K) , and negative for Islet1 (N) . Nuclei were stained with DAPI and superimposed with staining for nestin and TRPV1 (C) , Peripherin (F) , BRN3A (I) , Pax6 (L) , and Islet1 (O) . Calibration bar = 100 μm. Quantification of positive cells for each marker relative to DAPI-stained nuclei, n = 2–3 independent experiments (P) .
    Figure Legend Snippet: Characterization of NCPCs with specific markers. On day 10, cells presented neural progenitor cell’s morphology and positive staining for Nestin (A,D,G,J,M) , TRPV1 (B) , Peripherin (E) , BRN3A (H) , Pax6 (K) , and negative for Islet1 (N) . Nuclei were stained with DAPI and superimposed with staining for nestin and TRPV1 (C) , Peripherin (F) , BRN3A (I) , Pax6 (L) , and Islet1 (O) . Calibration bar = 100 μm. Quantification of positive cells for each marker relative to DAPI-stained nuclei, n = 2–3 independent experiments (P) .

    Techniques Used: Staining, Marker

    5) Product Images from "Generation of iPSC-Derived Human Peripheral Sensory Neurons Releasing Substance P Elicited by TRPV1 Agonists"

    Article Title: Generation of iPSC-Derived Human Peripheral Sensory Neurons Releasing Substance P Elicited by TRPV1 Agonists

    Journal: Frontiers in Molecular Neuroscience

    doi: 10.3389/fnmol.2018.00277

    Characterization of NCPCs with specific markers. On day 10, cells presented neural progenitor cell’s morphology and positive staining for Nestin (A,D,G,J,M) , TRPV1 (B) , Peripherin (E) , BRN3A (H) , Pax6 (K) , and negative for Islet1 (N) . Nuclei were stained with DAPI and superimposed with staining for nestin and TRPV1 (C) , Peripherin (F) , BRN3A (I) , Pax6 (L) , and Islet1 (O) . Calibration bar = 100 μm. Quantification of positive cells for each marker relative to DAPI-stained nuclei, n = 2–3 independent experiments (P) .
    Figure Legend Snippet: Characterization of NCPCs with specific markers. On day 10, cells presented neural progenitor cell’s morphology and positive staining for Nestin (A,D,G,J,M) , TRPV1 (B) , Peripherin (E) , BRN3A (H) , Pax6 (K) , and negative for Islet1 (N) . Nuclei were stained with DAPI and superimposed with staining for nestin and TRPV1 (C) , Peripherin (F) , BRN3A (I) , Pax6 (L) , and Islet1 (O) . Calibration bar = 100 μm. Quantification of positive cells for each marker relative to DAPI-stained nuclei, n = 2–3 independent experiments (P) .

    Techniques Used: Staining, Marker

    6) Product Images from "A cellular model for sporadic ALS using patient-derived induced pluripotent stem cells"

    Article Title: A cellular model for sporadic ALS using patient-derived induced pluripotent stem cells

    Journal: Molecular and cellular neurosciences

    doi: 10.1016/j.mcn.2013.07.007

    TDP-43 localization in patient iPSC-derived motor neurons (A) iPSC-MN stained for TDP-43 shows TDP-43 is ubiquitously expressed and nuclear in all cells including in ISLET1-positive neurons from control (left panel) and ALS (right panel) patients. ISLET1-positive cells from sporadic ALS iPSC-MN have nuclear staining as well as nuclear aggregates that stain with higher intensity for TDP-43 (arrowheads, right panel) but aggregates are not present in healthy controls (left panel). Fibroblasts from healthy control and sALS patients do not show nuclear aggregates. Scale bar: 30 µm. (B) Sporadic ALS patient-derived iPSC-MN cells stained with nuclear envelop marker LAMIN-A (green) and TDP-43 (red) shows TDP-43 aggregates are inside the nuclear envelope; scale bar: 20µm. (C) Quantification of healthy control IPRN.0013 and sALS IPRN.0048 clone 1 iPSC-MN cultures shows TDP-43 aggregation is present in higher fraction of ISLET1 or HB9-positive cells compared to negative cells. (30.7% of ISLET1/HB9-positive motor neurons as compared to 16.2% of ISLET1/HB9-negative cells in sALS iPSC-MN cultures. Bars are standard deviation 9% and 8% respectively; P
    Figure Legend Snippet: TDP-43 localization in patient iPSC-derived motor neurons (A) iPSC-MN stained for TDP-43 shows TDP-43 is ubiquitously expressed and nuclear in all cells including in ISLET1-positive neurons from control (left panel) and ALS (right panel) patients. ISLET1-positive cells from sporadic ALS iPSC-MN have nuclear staining as well as nuclear aggregates that stain with higher intensity for TDP-43 (arrowheads, right panel) but aggregates are not present in healthy controls (left panel). Fibroblasts from healthy control and sALS patients do not show nuclear aggregates. Scale bar: 30 µm. (B) Sporadic ALS patient-derived iPSC-MN cells stained with nuclear envelop marker LAMIN-A (green) and TDP-43 (red) shows TDP-43 aggregates are inside the nuclear envelope; scale bar: 20µm. (C) Quantification of healthy control IPRN.0013 and sALS IPRN.0048 clone 1 iPSC-MN cultures shows TDP-43 aggregation is present in higher fraction of ISLET1 or HB9-positive cells compared to negative cells. (30.7% of ISLET1/HB9-positive motor neurons as compared to 16.2% of ISLET1/HB9-negative cells in sALS iPSC-MN cultures. Bars are standard deviation 9% and 8% respectively; P

    Techniques Used: Derivative Assay, Staining, Marker, Standard Deviation

    Patient-specific iPSC and iPSC-derived motor neurons Representative images of: (A) iPSC colonies from one control and one ALS patient in phase-contrast. (B) iPSC colonies stained with antibodies for pluripotency markers NANOG and TRA-1-60. (C) iPSC-MN cultures stained with nuclear marker DAPI (blue) and antibodies to motor neuron markers ISLET1 (red) and HB9 (green). (D) iPSC-MN cultures stained with antibody to axonal marker Neurofilament (SMI31). iPSC and iPSC-MN shown are from healthy control IPRN.0013 and fALS patient IPRN.0028. (Scale bars a-b: 200 µm, c-d: 75 µm). (E) (i-ii), Representative trace showing electrical activity of iPS-MN from a healthy individual (Scale bars: 100 mV; 100 ms. 8 neurons recorded from fired robust and repetitive action potentials (bursts) to depolarizing current steps. (Iii) Example trace showing action potential elicited by the rebound depolarization following 300 pA hyperpolarizing step for 100 ms (Scales bars: 100 mV; 100 ms) and example trace of spontaneous bursts of action potentials and spontaneous post-synaptic potentials from iPS derived motor neurons (50 mV; 3 Sec). (Insets) Examples of EPSP (scales: 3 mV; 250 ms), and IPSP (scales: 30 mV, 100 ms). (F) (i) Sporadic ALS iPSC-MN responses −10, 0, and 10 pA current steps for 300 msec (scale: 25 mV). (ii) APs elicited by rebound depolarization following hyperpolarizing current step (300 pA; 300 msec). (iii) Spontaneous activity of sALS iPSC-MN (scale bars; 50 mV; 500 msec) (insets example of sEPSP and sIPSP (scale bars: 5 mV; 200 msec))( n = 7).
    Figure Legend Snippet: Patient-specific iPSC and iPSC-derived motor neurons Representative images of: (A) iPSC colonies from one control and one ALS patient in phase-contrast. (B) iPSC colonies stained with antibodies for pluripotency markers NANOG and TRA-1-60. (C) iPSC-MN cultures stained with nuclear marker DAPI (blue) and antibodies to motor neuron markers ISLET1 (red) and HB9 (green). (D) iPSC-MN cultures stained with antibody to axonal marker Neurofilament (SMI31). iPSC and iPSC-MN shown are from healthy control IPRN.0013 and fALS patient IPRN.0028. (Scale bars a-b: 200 µm, c-d: 75 µm). (E) (i-ii), Representative trace showing electrical activity of iPS-MN from a healthy individual (Scale bars: 100 mV; 100 ms. 8 neurons recorded from fired robust and repetitive action potentials (bursts) to depolarizing current steps. (Iii) Example trace showing action potential elicited by the rebound depolarization following 300 pA hyperpolarizing step for 100 ms (Scales bars: 100 mV; 100 ms) and example trace of spontaneous bursts of action potentials and spontaneous post-synaptic potentials from iPS derived motor neurons (50 mV; 3 Sec). (Insets) Examples of EPSP (scales: 3 mV; 250 ms), and IPSP (scales: 30 mV, 100 ms). (F) (i) Sporadic ALS iPSC-MN responses −10, 0, and 10 pA current steps for 300 msec (scale: 25 mV). (ii) APs elicited by rebound depolarization following hyperpolarizing current step (300 pA; 300 msec). (iii) Spontaneous activity of sALS iPSC-MN (scale bars; 50 mV; 500 msec) (insets example of sEPSP and sIPSP (scale bars: 5 mV; 200 msec))( n = 7).

    Techniques Used: Derivative Assay, Staining, Marker, Activity Assay, Mass Spectrometry, Size-exclusion Chromatography

    7) Product Images from "A cellular model for sporadic ALS using patient-derived induced pluripotent stem cells"

    Article Title: A cellular model for sporadic ALS using patient-derived induced pluripotent stem cells

    Journal: Molecular and cellular neurosciences

    doi: 10.1016/j.mcn.2013.07.007

    TDP-43 localization in patient iPSC-derived motor neurons (A) iPSC-MN stained for TDP-43 shows TDP-43 is ubiquitously expressed and nuclear in all cells including in ISLET1-positive neurons from control (left panel) and ALS (right panel) patients. ISLET1-positive cells from sporadic ALS iPSC-MN have nuclear staining as well as nuclear aggregates that stain with higher intensity for TDP-43 (arrowheads, right panel) but aggregates are not present in healthy controls (left panel). Fibroblasts from healthy control and sALS patients do not show nuclear aggregates. Scale bar: 30 µm. (B) Sporadic ALS patient-derived iPSC-MN cells stained with nuclear envelop marker LAMIN-A (green) and TDP-43 (red) shows TDP-43 aggregates are inside the nuclear envelope; scale bar: 20µm. (C) Quantification of healthy control IPRN.0013 and sALS IPRN.0048 clone 1 iPSC-MN cultures shows TDP-43 aggregation is present in higher fraction of ISLET1 or HB9-positive cells compared to negative cells. (30.7% of ISLET1/HB9-positive motor neurons as compared to 16.2% of ISLET1/HB9-negative cells in sALS iPSC-MN cultures. Bars are standard deviation 9% and 8% respectively; P
    Figure Legend Snippet: TDP-43 localization in patient iPSC-derived motor neurons (A) iPSC-MN stained for TDP-43 shows TDP-43 is ubiquitously expressed and nuclear in all cells including in ISLET1-positive neurons from control (left panel) and ALS (right panel) patients. ISLET1-positive cells from sporadic ALS iPSC-MN have nuclear staining as well as nuclear aggregates that stain with higher intensity for TDP-43 (arrowheads, right panel) but aggregates are not present in healthy controls (left panel). Fibroblasts from healthy control and sALS patients do not show nuclear aggregates. Scale bar: 30 µm. (B) Sporadic ALS patient-derived iPSC-MN cells stained with nuclear envelop marker LAMIN-A (green) and TDP-43 (red) shows TDP-43 aggregates are inside the nuclear envelope; scale bar: 20µm. (C) Quantification of healthy control IPRN.0013 and sALS IPRN.0048 clone 1 iPSC-MN cultures shows TDP-43 aggregation is present in higher fraction of ISLET1 or HB9-positive cells compared to negative cells. (30.7% of ISLET1/HB9-positive motor neurons as compared to 16.2% of ISLET1/HB9-negative cells in sALS iPSC-MN cultures. Bars are standard deviation 9% and 8% respectively; P

    Techniques Used: Derivative Assay, Staining, Marker, Standard Deviation

    Patient-specific iPSC and iPSC-derived motor neurons Representative images of: (A) iPSC colonies from one control and one ALS patient in phase-contrast. (B) iPSC colonies stained with antibodies for pluripotency markers NANOG and TRA-1-60. (C) iPSC-MN cultures stained with nuclear marker DAPI (blue) and antibodies to motor neuron markers ISLET1 (red) and HB9 (green). (D) iPSC-MN cultures stained with antibody to axonal marker Neurofilament (SMI31). iPSC and iPSC-MN shown are from healthy control IPRN.0013 and fALS patient IPRN.0028. (Scale bars a-b: 200 µm, c-d: 75 µm). (E) (i-ii), Representative trace showing electrical activity of iPS-MN from a healthy individual (Scale bars: 100 mV; 100 ms. 8 neurons recorded from fired robust and repetitive action potentials (bursts) to depolarizing current steps. (Iii) Example trace showing action potential elicited by the rebound depolarization following 300 pA hyperpolarizing step for 100 ms (Scales bars: 100 mV; 100 ms) and example trace of spontaneous bursts of action potentials and spontaneous post-synaptic potentials from iPS derived motor neurons (50 mV; 3 Sec). (Insets) Examples of EPSP (scales: 3 mV; 250 ms), and IPSP (scales: 30 mV, 100 ms). (F) (i) Sporadic ALS iPSC-MN responses −10, 0, and 10 pA current steps for 300 msec (scale: 25 mV). (ii) APs elicited by rebound depolarization following hyperpolarizing current step (300 pA; 300 msec). (iii) Spontaneous activity of sALS iPSC-MN (scale bars; 50 mV; 500 msec) (insets example of sEPSP and sIPSP (scale bars: 5 mV; 200 msec))( n = 7).
    Figure Legend Snippet: Patient-specific iPSC and iPSC-derived motor neurons Representative images of: (A) iPSC colonies from one control and one ALS patient in phase-contrast. (B) iPSC colonies stained with antibodies for pluripotency markers NANOG and TRA-1-60. (C) iPSC-MN cultures stained with nuclear marker DAPI (blue) and antibodies to motor neuron markers ISLET1 (red) and HB9 (green). (D) iPSC-MN cultures stained with antibody to axonal marker Neurofilament (SMI31). iPSC and iPSC-MN shown are from healthy control IPRN.0013 and fALS patient IPRN.0028. (Scale bars a-b: 200 µm, c-d: 75 µm). (E) (i-ii), Representative trace showing electrical activity of iPS-MN from a healthy individual (Scale bars: 100 mV; 100 ms. 8 neurons recorded from fired robust and repetitive action potentials (bursts) to depolarizing current steps. (Iii) Example trace showing action potential elicited by the rebound depolarization following 300 pA hyperpolarizing step for 100 ms (Scales bars: 100 mV; 100 ms) and example trace of spontaneous bursts of action potentials and spontaneous post-synaptic potentials from iPS derived motor neurons (50 mV; 3 Sec). (Insets) Examples of EPSP (scales: 3 mV; 250 ms), and IPSP (scales: 30 mV, 100 ms). (F) (i) Sporadic ALS iPSC-MN responses −10, 0, and 10 pA current steps for 300 msec (scale: 25 mV). (ii) APs elicited by rebound depolarization following hyperpolarizing current step (300 pA; 300 msec). (iii) Spontaneous activity of sALS iPSC-MN (scale bars; 50 mV; 500 msec) (insets example of sEPSP and sIPSP (scale bars: 5 mV; 200 msec))( n = 7).

    Techniques Used: Derivative Assay, Staining, Marker, Activity Assay, Mass Spectrometry, Size-exclusion Chromatography

    8) Product Images from "Generation of iPSC-Derived Human Peripheral Sensory Neurons Releasing Substance P Elicited by TRPV1 Agonists"

    Article Title: Generation of iPSC-Derived Human Peripheral Sensory Neurons Releasing Substance P Elicited by TRPV1 Agonists

    Journal: Frontiers in Molecular Neuroscience

    doi: 10.3389/fnmol.2018.00277

    Characterization of NCPCs with specific markers. On day 10, cells presented neural progenitor cell’s morphology and positive staining for Nestin (A,D,G,J,M) , TRPV1 (B) , Peripherin (E) , BRN3A (H) , Pax6 (K) , and negative for Islet1 (N) . Nuclei were stained with DAPI and superimposed with staining for nestin and TRPV1 (C) , Peripherin (F) , BRN3A (I) , Pax6 (L) , and Islet1 (O) . Calibration bar = 100 μm. Quantification of positive cells for each marker relative to DAPI-stained nuclei, n = 2–3 independent experiments (P) .
    Figure Legend Snippet: Characterization of NCPCs with specific markers. On day 10, cells presented neural progenitor cell’s morphology and positive staining for Nestin (A,D,G,J,M) , TRPV1 (B) , Peripherin (E) , BRN3A (H) , Pax6 (K) , and negative for Islet1 (N) . Nuclei were stained with DAPI and superimposed with staining for nestin and TRPV1 (C) , Peripherin (F) , BRN3A (I) , Pax6 (L) , and Islet1 (O) . Calibration bar = 100 μm. Quantification of positive cells for each marker relative to DAPI-stained nuclei, n = 2–3 independent experiments (P) .

    Techniques Used: Staining, Marker

    9) Product Images from "Derivation and Identification of Motor Neurons from Human Urine-Derived Induced Pluripotent Stem Cells"

    Article Title: Derivation and Identification of Motor Neurons from Human Urine-Derived Induced Pluripotent Stem Cells

    Journal: Stem Cells International

    doi: 10.1155/2018/3628578

    Immunocytochemical staining and ratios of positive labeling of the MNPs and MNs. (a) After 13 days of induction, cells derived from all three iPSC lines, UC005, UE017, and B-iPSC, were positive for the MNP markers, Olig2 and Pax6. Cell nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI). The scale bar is 50 μ m. (b) After 19 days of induction, the cells derived from all three iPSC lines, UC005, UE017, and B-iPSC, were positive for the MN markers, HB9 and Islet1. Cell nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI). The scale bar is 50 μ m. (c) Cells from the UC005 and B-iPSC lines expressed higher levels of the MNP markers than the cells from the UE017 line. (d) The ratios of positive labeling for HB9 and Islet1 in MNPs after 19 days of induction. The ratios of positive labeling for HB9 were 79%, 74%, and 81% in MNs derived from UC005, UE017, and B-iPSC, respectively, but the difference was not statistically significant. A total of 84% of MNs from B-iPSC expressed Islet1, which was significantly higher than that of MNs from UC005 and UE017. The ratio of positive labeling (%) = (number of positive cells/total number) × 100%. ∗∗∗ P
    Figure Legend Snippet: Immunocytochemical staining and ratios of positive labeling of the MNPs and MNs. (a) After 13 days of induction, cells derived from all three iPSC lines, UC005, UE017, and B-iPSC, were positive for the MNP markers, Olig2 and Pax6. Cell nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI). The scale bar is 50 μ m. (b) After 19 days of induction, the cells derived from all three iPSC lines, UC005, UE017, and B-iPSC, were positive for the MN markers, HB9 and Islet1. Cell nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI). The scale bar is 50 μ m. (c) Cells from the UC005 and B-iPSC lines expressed higher levels of the MNP markers than the cells from the UE017 line. (d) The ratios of positive labeling for HB9 and Islet1 in MNPs after 19 days of induction. The ratios of positive labeling for HB9 were 79%, 74%, and 81% in MNs derived from UC005, UE017, and B-iPSC, respectively, but the difference was not statistically significant. A total of 84% of MNs from B-iPSC expressed Islet1, which was significantly higher than that of MNs from UC005 and UE017. The ratio of positive labeling (%) = (number of positive cells/total number) × 100%. ∗∗∗ P

    Techniques Used: Staining, Labeling, Derivative Assay

    10) Product Images from "Generation of iPSC-Derived Human Peripheral Sensory Neurons Releasing Substance P Elicited by TRPV1 Agonists"

    Article Title: Generation of iPSC-Derived Human Peripheral Sensory Neurons Releasing Substance P Elicited by TRPV1 Agonists

    Journal: Frontiers in Molecular Neuroscience

    doi: 10.3389/fnmol.2018.00277

    Characterization of NCPCs with specific markers. On day 10, cells presented neural progenitor cell’s morphology and positive staining for Nestin (A,D,G,J,M) , TRPV1 (B) , Peripherin (E) , BRN3A (H) , Pax6 (K) , and negative for Islet1 (N) . Nuclei were stained with DAPI and superimposed with staining for nestin and TRPV1 (C) , Peripherin (F) , BRN3A (I) , Pax6 (L) , and Islet1 (O) . Calibration bar = 100 μm. Quantification of positive cells for each marker relative to DAPI-stained nuclei, n = 2–3 independent experiments (P) .
    Figure Legend Snippet: Characterization of NCPCs with specific markers. On day 10, cells presented neural progenitor cell’s morphology and positive staining for Nestin (A,D,G,J,M) , TRPV1 (B) , Peripherin (E) , BRN3A (H) , Pax6 (K) , and negative for Islet1 (N) . Nuclei were stained with DAPI and superimposed with staining for nestin and TRPV1 (C) , Peripherin (F) , BRN3A (I) , Pax6 (L) , and Islet1 (O) . Calibration bar = 100 μm. Quantification of positive cells for each marker relative to DAPI-stained nuclei, n = 2–3 independent experiments (P) .

    Techniques Used: Staining, Marker

    11) Product Images from "Human Induced Pluripotent Cell‐Derived Sensory Neurons for Fate Commitment of Bone Marrow‐Derived Schwann Cells: Implications for Remyelination Therapy"

    Article Title: Human Induced Pluripotent Cell‐Derived Sensory Neurons for Fate Commitment of Bone Marrow‐Derived Schwann Cells: Implications for Remyelination Therapy

    Journal: Stem Cells Translational Medicine

    doi: 10.5966/sctm.2015-0424

    Stability of human iPSC‐derived sensory neurons in terms of marker expression and cell‐cycle profile. (A): Semiquantitative reverse transcription‐polymerase chain reaction analysis revealed sustained expression of mRNA for TUJ1, Islet1, peripherin, and BRN3A in iPSC‐iSNs on day 8 of SMI treatment (+SMIs) and those on day 14 of maintenance treatment with neurobasal medium and growth factors (+GFs) as shown with ethidium bromide‐stained gel patterns (Aa) and densitometric scans of the specific marker relative to that of GAPDH (Ab) . ∗∗, p
    Figure Legend Snippet: Stability of human iPSC‐derived sensory neurons in terms of marker expression and cell‐cycle profile. (A): Semiquantitative reverse transcription‐polymerase chain reaction analysis revealed sustained expression of mRNA for TUJ1, Islet1, peripherin, and BRN3A in iPSC‐iSNs on day 8 of SMI treatment (+SMIs) and those on day 14 of maintenance treatment with neurobasal medium and growth factors (+GFs) as shown with ethidium bromide‐stained gel patterns (Aa) and densitometric scans of the specific marker relative to that of GAPDH (Ab) . ∗∗, p

    Techniques Used: Derivative Assay, Marker, Expressing, Reverse Transcription Polymerase Chain Reaction, Staining

    One‐step protocol for the differentiation of hiPSCs to sensory neurons. (A): Schematic outline of the protocol. (B): Morphological changes in the progression from hiPSCs to sensory neurons as viewed under phase‐contrast microscopy. Scale bars = 50 μm. (C): Neuronal lineage marker expression by day‐8 cells as detected by double immunofluorescence for TUJ1 and BRN3A (Ca) , peripherin and neurofilament (Cb) , Islet1 and BRN3A (Cc) , and Islet1 and peripherin (Cd) . Scale bars = 50 μm. Abbreviations: hiPSC, human induced pluripotent stem cell; SN, sensory neuron.
    Figure Legend Snippet: One‐step protocol for the differentiation of hiPSCs to sensory neurons. (A): Schematic outline of the protocol. (B): Morphological changes in the progression from hiPSCs to sensory neurons as viewed under phase‐contrast microscopy. Scale bars = 50 μm. (C): Neuronal lineage marker expression by day‐8 cells as detected by double immunofluorescence for TUJ1 and BRN3A (Ca) , peripherin and neurofilament (Cb) , Islet1 and BRN3A (Cc) , and Islet1 and peripherin (Cd) . Scale bars = 50 μm. Abbreviations: hiPSC, human induced pluripotent stem cell; SN, sensory neuron.

    Techniques Used: Microscopy, Marker, Expressing, Immunofluorescence

    Continuing culture of human iPSC‐derived neurons in maintenance medium for 14 days. (A): Neuronal lineage marker expression by the iPSC‐derived neurons as detected by double immunofluorescence for TUJ1 and neurofilament (Aa) , peripherin and Islet1 (Ab) , and peripherin and BRN3A (Ac) . Scale bars = 50 μm. (B): Representative dot plots of flow cytometric data showing the percentage of iPSC‐derived cells positive for TUJ1 (91.41%), neurofilament (92.39%), Islet1 (80.17%), and NeuN (74.65%) but not for PAX6, Nestin, AP2, and HNK1 (
    Figure Legend Snippet: Continuing culture of human iPSC‐derived neurons in maintenance medium for 14 days. (A): Neuronal lineage marker expression by the iPSC‐derived neurons as detected by double immunofluorescence for TUJ1 and neurofilament (Aa) , peripherin and Islet1 (Ab) , and peripherin and BRN3A (Ac) . Scale bars = 50 μm. (B): Representative dot plots of flow cytometric data showing the percentage of iPSC‐derived cells positive for TUJ1 (91.41%), neurofilament (92.39%), Islet1 (80.17%), and NeuN (74.65%) but not for PAX6, Nestin, AP2, and HNK1 (

    Techniques Used: Derivative Assay, Marker, Expressing, Immunofluorescence, Flow Cytometry

    12) Product Images from "Generation of iPSC-Derived Human Peripheral Sensory Neurons Releasing Substance P Elicited by TRPV1 Agonists"

    Article Title: Generation of iPSC-Derived Human Peripheral Sensory Neurons Releasing Substance P Elicited by TRPV1 Agonists

    Journal: Frontiers in Molecular Neuroscience

    doi: 10.3389/fnmol.2018.00277

    Characterization of NCPCs with specific markers. On day 10, cells presented neural progenitor cell’s morphology and positive staining for Nestin (A,D,G,J,M) , TRPV1 (B) , Peripherin (E) , BRN3A (H) , Pax6 (K) , and negative for Islet1 (N) . Nuclei were stained with DAPI and superimposed with staining for nestin and TRPV1 (C) , Peripherin (F) , BRN3A (I) , Pax6 (L) , and Islet1 (O) . Calibration bar = 100 μm. Quantification of positive cells for each marker relative to DAPI-stained nuclei, n = 2–3 independent experiments (P) .
    Figure Legend Snippet: Characterization of NCPCs with specific markers. On day 10, cells presented neural progenitor cell’s morphology and positive staining for Nestin (A,D,G,J,M) , TRPV1 (B) , Peripherin (E) , BRN3A (H) , Pax6 (K) , and negative for Islet1 (N) . Nuclei were stained with DAPI and superimposed with staining for nestin and TRPV1 (C) , Peripherin (F) , BRN3A (I) , Pax6 (L) , and Islet1 (O) . Calibration bar = 100 μm. Quantification of positive cells for each marker relative to DAPI-stained nuclei, n = 2–3 independent experiments (P) .

    Techniques Used: Staining, Marker

    13) Product Images from "Generation of iPSC-Derived Human Peripheral Sensory Neurons Releasing Substance P Elicited by TRPV1 Agonists"

    Article Title: Generation of iPSC-Derived Human Peripheral Sensory Neurons Releasing Substance P Elicited by TRPV1 Agonists

    Journal: Frontiers in Molecular Neuroscience

    doi: 10.3389/fnmol.2018.00277

    Characterization of NCPCs with specific markers. On day 10, cells presented neural progenitor cell’s morphology and positive staining for Nestin (A,D,G,J,M) , TRPV1 (B) , Peripherin (E) , BRN3A (H) , Pax6 (K) , and negative for Islet1 (N) . Nuclei were stained with DAPI and superimposed with staining for nestin and TRPV1 (C) , Peripherin (F) , BRN3A (I) , Pax6 (L) , and Islet1 (O) . Calibration bar = 100 μm. Quantification of positive cells for each marker relative to DAPI-stained nuclei, n = 2–3 independent experiments (P) .
    Figure Legend Snippet: Characterization of NCPCs with specific markers. On day 10, cells presented neural progenitor cell’s morphology and positive staining for Nestin (A,D,G,J,M) , TRPV1 (B) , Peripherin (E) , BRN3A (H) , Pax6 (K) , and negative for Islet1 (N) . Nuclei were stained with DAPI and superimposed with staining for nestin and TRPV1 (C) , Peripherin (F) , BRN3A (I) , Pax6 (L) , and Islet1 (O) . Calibration bar = 100 μm. Quantification of positive cells for each marker relative to DAPI-stained nuclei, n = 2–3 independent experiments (P) .

    Techniques Used: Staining, Marker

    14) Product Images from "Generation of iPSC-Derived Human Peripheral Sensory Neurons Releasing Substance P Elicited by TRPV1 Agonists"

    Article Title: Generation of iPSC-Derived Human Peripheral Sensory Neurons Releasing Substance P Elicited by TRPV1 Agonists

    Journal: Frontiers in Molecular Neuroscience

    doi: 10.3389/fnmol.2018.00277

    Characterization of NCPCs with specific markers. On day 10, cells presented neural progenitor cell’s morphology and positive staining for Nestin (A,D,G,J,M) , TRPV1 (B) , Peripherin (E) , BRN3A (H) , Pax6 (K) , and negative for Islet1 (N) . Nuclei were stained with DAPI and superimposed with staining for nestin and TRPV1 (C) , Peripherin (F) , BRN3A (I) , Pax6 (L) , and Islet1 (O) . Calibration bar = 100 μm. Quantification of positive cells for each marker relative to DAPI-stained nuclei, n = 2–3 independent experiments (P) .
    Figure Legend Snippet: Characterization of NCPCs with specific markers. On day 10, cells presented neural progenitor cell’s morphology and positive staining for Nestin (A,D,G,J,M) , TRPV1 (B) , Peripherin (E) , BRN3A (H) , Pax6 (K) , and negative for Islet1 (N) . Nuclei were stained with DAPI and superimposed with staining for nestin and TRPV1 (C) , Peripherin (F) , BRN3A (I) , Pax6 (L) , and Islet1 (O) . Calibration bar = 100 μm. Quantification of positive cells for each marker relative to DAPI-stained nuclei, n = 2–3 independent experiments (P) .

    Techniques Used: Staining, Marker

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    Abcam islet1
    Characterization of NCPCs with specific markers. On day 10, cells presented neural progenitor cell’s morphology and positive staining for Nestin (A,D,G,J,M) , TRPV1 (B) , Peripherin (E) , BRN3A (H) , Pax6 (K) , and negative for <t>Islet1</t> (N) . Nuclei were stained with DAPI and superimposed with staining for nestin and TRPV1 (C) , Peripherin (F) , BRN3A (I) , Pax6 (L) , and Islet1 (O) . Calibration bar = 100 μm. Quantification of positive cells for each marker relative to DAPI-stained nuclei, n = 2–3 independent experiments (P) .
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    Analysis of epistasis between Brn3a and <t>Islet1</t> mutations
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    Characterization of NCPCs with specific markers. On day 10, cells presented neural progenitor cell’s morphology and positive staining for Nestin (A,D,G,J,M) , TRPV1 (B) , Peripherin (E) , BRN3A (H) , Pax6 (K) , and negative for Islet1 (N) . Nuclei were stained with DAPI and superimposed with staining for nestin and TRPV1 (C) , Peripherin (F) , BRN3A (I) , Pax6 (L) , and Islet1 (O) . Calibration bar = 100 μm. Quantification of positive cells for each marker relative to DAPI-stained nuclei, n = 2–3 independent experiments (P) .

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Generation of iPSC-Derived Human Peripheral Sensory Neurons Releasing Substance P Elicited by TRPV1 Agonists

    doi: 10.3389/fnmol.2018.00277

    Figure Lengend Snippet: Characterization of NCPCs with specific markers. On day 10, cells presented neural progenitor cell’s morphology and positive staining for Nestin (A,D,G,J,M) , TRPV1 (B) , Peripherin (E) , BRN3A (H) , Pax6 (K) , and negative for Islet1 (N) . Nuclei were stained with DAPI and superimposed with staining for nestin and TRPV1 (C) , Peripherin (F) , BRN3A (I) , Pax6 (L) , and Islet1 (O) . Calibration bar = 100 μm. Quantification of positive cells for each marker relative to DAPI-stained nuclei, n = 2–3 independent experiments (P) .

    Article Snippet: The expression of all these markers, except Islet1, was also detected among RNA-Seq transcripts (see Figure ).

    Techniques: Staining, Marker

    Characterization of NCPCs with specific markers. On day 10, cells presented neural progenitor cell’s morphology and positive staining for Nestin (A,D,G,J,M) , TRPV1 (B) , Peripherin (E) , BRN3A (H) , Pax6 (K) , and negative for Islet1 (N) . Nuclei were stained with DAPI and superimposed with staining for nestin and TRPV1 (C) , Peripherin (F) , BRN3A (I) , Pax6 (L) , and Islet1 (O) . Calibration bar = 100 μm. Quantification of positive cells for each marker relative to DAPI-stained nuclei, n = 2–3 independent experiments (P) .

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Generation of iPSC-Derived Human Peripheral Sensory Neurons Releasing Substance P Elicited by TRPV1 Agonists

    doi: 10.3389/fnmol.2018.00277

    Figure Lengend Snippet: Characterization of NCPCs with specific markers. On day 10, cells presented neural progenitor cell’s morphology and positive staining for Nestin (A,D,G,J,M) , TRPV1 (B) , Peripherin (E) , BRN3A (H) , Pax6 (K) , and negative for Islet1 (N) . Nuclei were stained with DAPI and superimposed with staining for nestin and TRPV1 (C) , Peripherin (F) , BRN3A (I) , Pax6 (L) , and Islet1 (O) . Calibration bar = 100 μm. Quantification of positive cells for each marker relative to DAPI-stained nuclei, n = 2–3 independent experiments (P) .

    Article Snippet: We assessed the expression of TRPV1 ( Figures – ), Peripherin ( Figures – ), and Islet1 ( Figures – ).

    Techniques: Staining, Marker

    Analysis of epistasis between Brn3a and Islet1 mutations

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Brn3a and Islet1 act epistatically to regulate the gene expression program of sensory differentiation

    doi: 10.1523/JNEUROSCI.0901-11.2011

    Figure Lengend Snippet: Analysis of epistasis between Brn3a and Islet1 mutations

    Article Snippet: Other antisera included: rabbit anti-Etv1/Er81 and anti-Runx3 (gifts of S. Arber, ; ); rabbit anti-Runx1 (gift of T. Jessell), anti-DRG11 (gift of D. Lima, ), anti-caspase-3 (Cell Signal Tech), anti-peripherin (Millipore) and anti-Islet1 (Abcam); guinea pig anti-Sox 10 (gift of M. Wegner), and anti-Islet2 (gift of S. Pfaff, ); goat anti-TrkB and anti-TrkC (R & D Systems), anti-cRET (Fitzgerald) and anti-βgalactosidase (Biogenesis), and rabbit monoclonal anti-tubulinβ3 (Covance).

    Techniques:

    The Brn3a/Islet1 compound sensory phenotype at mid-gestation

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Brn3a and Islet1 act epistatically to regulate the gene expression program of sensory differentiation

    doi: 10.1523/JNEUROSCI.0901-11.2011

    Figure Lengend Snippet: The Brn3a/Islet1 compound sensory phenotype at mid-gestation

    Article Snippet: Other antisera included: rabbit anti-Etv1/Er81 and anti-Runx3 (gifts of S. Arber, ; ); rabbit anti-Runx1 (gift of T. Jessell), anti-DRG11 (gift of D. Lima, ), anti-caspase-3 (Cell Signal Tech), anti-peripherin (Millipore) and anti-Islet1 (Abcam); guinea pig anti-Sox 10 (gift of M. Wegner), and anti-Islet2 (gift of S. Pfaff, ); goat anti-TrkB and anti-TrkC (R & D Systems), anti-cRET (Fitzgerald) and anti-βgalactosidase (Biogenesis), and rabbit monoclonal anti-tubulinβ3 (Covance).

    Techniques:

    Immunofluorescence and in situ hybridization of novel Brn3a/Islet1 DKO targets

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Brn3a and Islet1 act epistatically to regulate the gene expression program of sensory differentiation

    doi: 10.1523/JNEUROSCI.0901-11.2011

    Figure Lengend Snippet: Immunofluorescence and in situ hybridization of novel Brn3a/Islet1 DKO targets

    Article Snippet: Other antisera included: rabbit anti-Etv1/Er81 and anti-Runx3 (gifts of S. Arber, ; ); rabbit anti-Runx1 (gift of T. Jessell), anti-DRG11 (gift of D. Lima, ), anti-caspase-3 (Cell Signal Tech), anti-peripherin (Millipore) and anti-Islet1 (Abcam); guinea pig anti-Sox 10 (gift of M. Wegner), and anti-Islet2 (gift of S. Pfaff, ); goat anti-TrkB and anti-TrkC (R & D Systems), anti-cRET (Fitzgerald) and anti-βgalactosidase (Biogenesis), and rabbit monoclonal anti-tubulinβ3 (Covance).

    Techniques: Immunofluorescence, In Situ Hybridization

    Chromatin immunoprecipitation of Islet1 bound to the Neurod4 locus

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Brn3a and Islet1 act epistatically to regulate the gene expression program of sensory differentiation

    doi: 10.1523/JNEUROSCI.0901-11.2011

    Figure Lengend Snippet: Chromatin immunoprecipitation of Islet1 bound to the Neurod4 locus

    Article Snippet: Other antisera included: rabbit anti-Etv1/Er81 and anti-Runx3 (gifts of S. Arber, ; ); rabbit anti-Runx1 (gift of T. Jessell), anti-DRG11 (gift of D. Lima, ), anti-caspase-3 (Cell Signal Tech), anti-peripherin (Millipore) and anti-Islet1 (Abcam); guinea pig anti-Sox 10 (gift of M. Wegner), and anti-Islet2 (gift of S. Pfaff, ); goat anti-TrkB and anti-TrkC (R & D Systems), anti-cRET (Fitzgerald) and anti-βgalactosidase (Biogenesis), and rabbit monoclonal anti-tubulinβ3 (Covance).

    Techniques: Chromatin Immunoprecipitation

    Displacement of cranial DRG and loss of peripheral sensory projections in Brn3a/Islet1 DKO embryos

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Brn3a and Islet1 act epistatically to regulate the gene expression program of sensory differentiation

    doi: 10.1523/JNEUROSCI.0901-11.2011

    Figure Lengend Snippet: Displacement of cranial DRG and loss of peripheral sensory projections in Brn3a/Islet1 DKO embryos

    Article Snippet: Other antisera included: rabbit anti-Etv1/Er81 and anti-Runx3 (gifts of S. Arber, ; ); rabbit anti-Runx1 (gift of T. Jessell), anti-DRG11 (gift of D. Lima, ), anti-caspase-3 (Cell Signal Tech), anti-peripherin (Millipore) and anti-Islet1 (Abcam); guinea pig anti-Sox 10 (gift of M. Wegner), and anti-Islet2 (gift of S. Pfaff, ); goat anti-TrkB and anti-TrkC (R & D Systems), anti-cRET (Fitzgerald) and anti-βgalactosidase (Biogenesis), and rabbit monoclonal anti-tubulinβ3 (Covance).

    Techniques:

    Loss of subtype specification in Brn3a/Islet1 DKO embryos

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Brn3a and Islet1 act epistatically to regulate the gene expression program of sensory differentiation

    doi: 10.1523/JNEUROSCI.0901-11.2011

    Figure Lengend Snippet: Loss of subtype specification in Brn3a/Islet1 DKO embryos

    Article Snippet: Other antisera included: rabbit anti-Etv1/Er81 and anti-Runx3 (gifts of S. Arber, ; ); rabbit anti-Runx1 (gift of T. Jessell), anti-DRG11 (gift of D. Lima, ), anti-caspase-3 (Cell Signal Tech), anti-peripherin (Millipore) and anti-Islet1 (Abcam); guinea pig anti-Sox 10 (gift of M. Wegner), and anti-Islet2 (gift of S. Pfaff, ); goat anti-TrkB and anti-TrkC (R & D Systems), anti-cRET (Fitzgerald) and anti-βgalactosidase (Biogenesis), and rabbit monoclonal anti-tubulinβ3 (Covance).

    Techniques:

    Sox13 overexpression partly antagonizes Phox2b activity. (a-m

    Journal: Neural Development

    Article Title: Identification of Phox2b-regulated genes by expression profiling of cranial motoneuron precursors

    doi: 10.1186/1749-8104-3-14

    Figure Lengend Snippet: Sox13 overexpression partly antagonizes Phox2b activity. (a-m") Chicken neural tubes electroporated at HH 12–13 either separately with pCAGGS::Phox2b-IRES-EGFP (a-a", d-d", g-g", j-j") or pCAGGS::Sox13-IRES-EGFP (b-b", e-e", h-h", k-k") or with pCAGGS::Phox2b-IRES-EGFP plus pCAGGS::Sox13-IRES-EGFP (c-c", f-f", i-i", l-l", m-m") were analyzed 48 h after electroporation. Transverse sections were stained with anti-GFP antibodies in combination with anti-NeuN (a-c"), anti-Sox2 (g-i"), anti-Islet1/2 (j-l") or anti-Lhx1,5 immunohistochemistry (m-m") or in combination with anti-BrdU antibodies (d-f") after BrdU injection into the amniotic cavity 2 h before fixation. Phox2b induces and Sox13 represses NeuN (asterisks in (a', a", b')) in the ML. When co-expressed with Sox13 , Phox2b is still capable of inducing NeuN, which, however, is now also switched on in the VZ (c', c"). Cells electroporated with Phox2b are BrdU-negative whether Sox13 was co-transfected (f) or not (d). Cells electroporated with Sox13 whether co-transfected with Phox2b (i) or not (h) express Sox2, while cells electroporated with Phox2b alone are always Sox2-negative (g). Islet1,2 induction by Phox2b (j) is abolished by co-transfection of Sox13 (l). Repression of Lhx1,5 by Phox2b is not prevented by co-expressing Sox13 (m). Co-transfection of Sox13 together with Phox2b inhibits relocation to the ML (c, f, i, l, m) implemented by expression of Phox2b alone (a, d, g, j).

    Article Snippet: The following antibodies were used: rabbit anti-axonin-1 [ ], mouse monoclonal anti-BrdU (1/100; Sigma), mouse monoclonal (Roche, Basel, Switzerland) and rabbit (Chemicon, Temecula, CA, USA) anti-GFP (1/400), mouse anti-Islet1,2 (1/100) [ ], rabbit anti-Islet1 (1/500; Abcam, Paris, France), mouse anti-Lhx1,5 (Developmental Studies Hybridoma Bank), mouse anti-NeuN (1/500), rabbit anti-Sox2 (1/1,000; Abcam), rabbit anti-Sox13 [ ] and adequate fluorescent secondary antibodies.

    Techniques: Over Expression, Activity Assay, Electroporation, Staining, Immunohistochemistry, Injection, Transfection, Cotransfection, Expressing

    Sfrp1 partially restores a commissural axonal phenotype. (a-c) Chicken neural tubes electroporated at HH12-13 with pCAGGS::Sfrp1-IRES-EGFP (a), pCAGGS::Phox2b-IRES-EGFP (b) or pCAGGS::Sfrp1-IRES-EGFP plus pCAGGS::Phox2b-IRES-EGFP (c) were analyzed by anti-GFP immunohistochemistry. Expression of Sfrp1 did not grossly alter the morphology of neuroepithelial cells, neither did it prevent Phox2b from promoting relocation to the ML. However, Sfrp1 prevented Phox2b from repressing the growth of commissural axons (arrowheads). (d-d

    Journal: Neural Development

    Article Title: Identification of Phox2b-regulated genes by expression profiling of cranial motoneuron precursors

    doi: 10.1186/1749-8104-3-14

    Figure Lengend Snippet: Sfrp1 partially restores a commissural axonal phenotype. (a-c) Chicken neural tubes electroporated at HH12-13 with pCAGGS::Sfrp1-IRES-EGFP (a), pCAGGS::Phox2b-IRES-EGFP (b) or pCAGGS::Sfrp1-IRES-EGFP plus pCAGGS::Phox2b-IRES-EGFP (c) were analyzed by anti-GFP immunohistochemistry. Expression of Sfrp1 did not grossly alter the morphology of neuroepithelial cells, neither did it prevent Phox2b from promoting relocation to the ML. However, Sfrp1 prevented Phox2b from repressing the growth of commissural axons (arrowheads). (d-d") Chicken neural tubes electroporated with pCAGGS::Sfrp1-IRES-EGFP and a Phox2b expression vector were analyzed by anti-GFP and anti-Phox2b immunohistochemistry as indicated. Virtually all the transfected cells express both GFP and Phox2b. (e-e") Chicken neural tubes electroporated with pCAGGS::Sfrp1-IRES-EGFP plus pCAGGS::Phox2b-IRES-EGFP were analyzed by anti-GFP and anti-Islet1,2 immunohistochemistry as indicated. Co-expression of Sfrp1 did not prevent Phox2b from inducing Islet1,2 (bracket). ( f-h') Chicken neural tubes electroporated with pCAGGS::Phox2b-IRES-EGFP (f, f'), pCAGGS::Sfrp1-IRES-EGFP (g, g') or with pCAGGS::Sfrp1-IRES-EGFP plus pCAGGS::Phox2b-IRES-EGFP (h, h') were analyzed by anti-axonin-1 and anti-GFP immunohistochemistry. In (f, g, h), the anti-axonin-1 staining is shown alone, and in (f', g', h') it is merged with the anti-GFP immunofluorescence. The fascicle formed by the commissural fibers en route to the floor plate is marked by an asterisk; it is absent after Phox2b transfection and partially restored by co-expressing Sfrp1 .

    Article Snippet: The following antibodies were used: rabbit anti-axonin-1 [ ], mouse monoclonal anti-BrdU (1/100; Sigma), mouse monoclonal (Roche, Basel, Switzerland) and rabbit (Chemicon, Temecula, CA, USA) anti-GFP (1/400), mouse anti-Islet1,2 (1/100) [ ], rabbit anti-Islet1 (1/500; Abcam, Paris, France), mouse anti-Lhx1,5 (Developmental Studies Hybridoma Bank), mouse anti-NeuN (1/500), rabbit anti-Sox2 (1/1,000; Abcam), rabbit anti-Sox13 [ ] and adequate fluorescent secondary antibodies.

    Techniques: Immunohistochemistry, Expressing, Plasmid Preparation, Transfection, Staining, Immunofluorescence