ion pair reverse phase hplc  (Millipore)


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    Name:
    High Performance Liquid Chromatography
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    Catalog Number:
    h8147
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    Structured Review

    Millipore ion pair reverse phase hplc

    https://www.bioz.com/result/ion pair reverse phase hplc/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ion pair reverse phase hplc - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Dual positional substrate specificity of rice allene oxide synthase-1: insight into mechanism of inhibition by type II ligand imidazole
    Article Snippet: .. Products (ketols and cyclopentanone derivatives) of 13-Soybean LOX/OsAOS1 reaction collected from normal phase HPLC were derivatized with Sigma-Sil-A (Sigma, USA) at 80℃ for 5 min. Products from CaLOX1/OsAOS1 reaction were initially methylated with (trimethylsilyl)diazomethane, and/or then derivatized with TMSiCl. .. Methylated and/or trimethylsilylated products were then analyzed by GC-MS (Elite-5MS, 0.25 mm × 30 m, 0.25 μm film thickness, Perkin Elmer, USA) with an injector temperature of 260℃.

    Article Title: A Novel Metagenomic Short-Chain Dehydrogenase/Reductase Attenuates Pseudomonas aeruginosa Biofilm Formation and Virulence on Caenorhabditis elegans
    Article Snippet: .. HPLC-MS analysis For chemical analysis 2 µmol 3-oxo-C12 -HSL (Sigma-Aldrich, Heidelberg, Germany) (4 mM final concentration in 0.5 ml total volume) was mixed with 1 mM NADPH and 100 µg purified BpiB09 protein in 100 mM potassium phosphate buffer pH 7.0 with 20% DMSO as cosolvent and incubated for 16 h at 30°C. .. After incubation, the resulting mixtures were extracted twice with ethyl acetate (total of 2 volumes) and the combined organic layers were concentrated in vacuo.

    Article Title: Highly modified and immunoactive N-glycans of the canine heartworm
    Article Snippet: .. HPLC fractionation For 1D-HPLC, complete pyridylaminated N-glycomes were fractionated by reversed-phase HPLC (Ascentis Express RP-amide from Sigma-Aldrich; 150 × 4.6 mm, 2.7 µm) and a gradient of 30% (v/v) methanol (buffer B) in 100 mM ammonium acetate, pH 4 (buffer A) was applied at a flow rate of 0.8 ml/min (Shimadzu LC-30 AD pumps) as follows: 0–4 min, 0% B; 4–14 min, 0–5% B; 14–24 min, 5–15% B; 24–34 min, 15–35% B; 34–35 min, return to starting conditions . .. The RP-amide HPLC column was calibrated daily in terms of glucose units using a pyridylaminated dextran hydrolysate and the degree of polymerisation of single standards was verified by MALDI-TOF–MS .

    Article Title: N-Acetyl Cysteine (NAC)-Directed Detoxification of Methacryloxylethyl Cetyl Ammonium Chloride (DMAE-CB)
    Article Snippet: .. Analysis of DMAE-CB-NAC adduct formation with high performance liquid chromatography (HPLC) Pure DMAE-CB was added into 10 mM NAC (Sigma; St Louis, MO, USA) solution (in water) to a final concentration of 8 mM. ..

    Article Title: Molecular breeding of a novel orange-brown tomato fruit with enhanced beta-carotene and chlorophyll accumulation
    Article Snippet: .. HPLC analysis Standards for β-carotene, lycopene, phytoene, and chlorophyll were purchased from Sigma- Aldrich (Sigma Co., USA). ..

    Article Title: The function of two P450s, CYP9M10 and CYP6AA7, in the permethrin resistance of Culex quinquefasciatus
    Article Snippet: .. HPLC analysis and permethrin, PBOH, and PBCHO metabolism study Permethrin, PBOH and PBCHO (HPLC grade, Sigma Aldrich) were initially dissolved in acetonitrile and PBCOOH (HPLC grade, Sigma Aldrich) in methanol to create 1 mM standard solutions. ..

    Article Title: Effects of Mlx-8, a phospholipase A2 from Brazilian coralsnake Micrurus lemniscatus venom, on muscarinic acetylcholine receptors in rat hippocampus
    Article Snippet: .. Drugs and Radiochemicals Carbachol (carbamylcholine chloride), lithium chloride, myo-inositol, pirenzepine (pirenzepine hydrochloride), HPLC grade acetonitrile, and trifluoroacetic acid were obtained from Sigma Chemical Co. (USA). .. The 7,7-dimethyl-5,8-eicosadienoic acid (DEDA) was obtained from Abcam Laboratories (USA).

    Hydrophilic Interaction Liquid Chromatography:

    Article Title: N-glycosylation of Colorectal Cancer Tissues
    Article Snippet: .. Five μl of dried and reconstituted HILIC-HPLC fractions were desalted using a C18 ZipTipTM (Millipore, Billerica, MA) following the manufacturer's instructions. .. AA-labeled glycans were eluted with 1.5 μl of 2,5-dihydroxybenzoic acid (10 mg/ml in 50/50, ACN/water containing 0.1% TFA) directly onto a stainless steel MALDI target plate and allowed to dry.

    Flow Cytometry:

    Article Title: Highly modified and immunoactive N-glycans of the canine heartworm
    Article Snippet: .. HPLC fractionation For 1D-HPLC, complete pyridylaminated N-glycomes were fractionated by reversed-phase HPLC (Ascentis Express RP-amide from Sigma-Aldrich; 150 × 4.6 mm, 2.7 µm) and a gradient of 30% (v/v) methanol (buffer B) in 100 mM ammonium acetate, pH 4 (buffer A) was applied at a flow rate of 0.8 ml/min (Shimadzu LC-30 AD pumps) as follows: 0–4 min, 0% B; 4–14 min, 0–5% B; 14–24 min, 5–15% B; 24–34 min, 15–35% B; 34–35 min, return to starting conditions . .. The RP-amide HPLC column was calibrated daily in terms of glucose units using a pyridylaminated dextran hydrolysate and the degree of polymerisation of single standards was verified by MALDI-TOF–MS .

    Methylation:

    Article Title: Dual positional substrate specificity of rice allene oxide synthase-1: insight into mechanism of inhibition by type II ligand imidazole
    Article Snippet: .. Products (ketols and cyclopentanone derivatives) of 13-Soybean LOX/OsAOS1 reaction collected from normal phase HPLC were derivatized with Sigma-Sil-A (Sigma, USA) at 80℃ for 5 min. Products from CaLOX1/OsAOS1 reaction were initially methylated with (trimethylsilyl)diazomethane, and/or then derivatized with TMSiCl. .. Methylated and/or trimethylsilylated products were then analyzed by GC-MS (Elite-5MS, 0.25 mm × 30 m, 0.25 μm film thickness, Perkin Elmer, USA) with an injector temperature of 260℃.

    Purification:

    Article Title: A Novel Metagenomic Short-Chain Dehydrogenase/Reductase Attenuates Pseudomonas aeruginosa Biofilm Formation and Virulence on Caenorhabditis elegans
    Article Snippet: .. HPLC-MS analysis For chemical analysis 2 µmol 3-oxo-C12 -HSL (Sigma-Aldrich, Heidelberg, Germany) (4 mM final concentration in 0.5 ml total volume) was mixed with 1 mM NADPH and 100 µg purified BpiB09 protein in 100 mM potassium phosphate buffer pH 7.0 with 20% DMSO as cosolvent and incubated for 16 h at 30°C. .. After incubation, the resulting mixtures were extracted twice with ethyl acetate (total of 2 volumes) and the combined organic layers were concentrated in vacuo.

    Concentration Assay:

    Article Title: A Novel Metagenomic Short-Chain Dehydrogenase/Reductase Attenuates Pseudomonas aeruginosa Biofilm Formation and Virulence on Caenorhabditis elegans
    Article Snippet: .. HPLC-MS analysis For chemical analysis 2 µmol 3-oxo-C12 -HSL (Sigma-Aldrich, Heidelberg, Germany) (4 mM final concentration in 0.5 ml total volume) was mixed with 1 mM NADPH and 100 µg purified BpiB09 protein in 100 mM potassium phosphate buffer pH 7.0 with 20% DMSO as cosolvent and incubated for 16 h at 30°C. .. After incubation, the resulting mixtures were extracted twice with ethyl acetate (total of 2 volumes) and the combined organic layers were concentrated in vacuo.

    Article Title: N-Acetyl Cysteine (NAC)-Directed Detoxification of Methacryloxylethyl Cetyl Ammonium Chloride (DMAE-CB)
    Article Snippet: .. Analysis of DMAE-CB-NAC adduct formation with high performance liquid chromatography (HPLC) Pure DMAE-CB was added into 10 mM NAC (Sigma; St Louis, MO, USA) solution (in water) to a final concentration of 8 mM. ..

    Incubation:

    Article Title: A Novel Metagenomic Short-Chain Dehydrogenase/Reductase Attenuates Pseudomonas aeruginosa Biofilm Formation and Virulence on Caenorhabditis elegans
    Article Snippet: .. HPLC-MS analysis For chemical analysis 2 µmol 3-oxo-C12 -HSL (Sigma-Aldrich, Heidelberg, Germany) (4 mM final concentration in 0.5 ml total volume) was mixed with 1 mM NADPH and 100 µg purified BpiB09 protein in 100 mM potassium phosphate buffer pH 7.0 with 20% DMSO as cosolvent and incubated for 16 h at 30°C. .. After incubation, the resulting mixtures were extracted twice with ethyl acetate (total of 2 volumes) and the combined organic layers were concentrated in vacuo.

    Fractionation:

    Article Title: Highly modified and immunoactive N-glycans of the canine heartworm
    Article Snippet: .. HPLC fractionation For 1D-HPLC, complete pyridylaminated N-glycomes were fractionated by reversed-phase HPLC (Ascentis Express RP-amide from Sigma-Aldrich; 150 × 4.6 mm, 2.7 µm) and a gradient of 30% (v/v) methanol (buffer B) in 100 mM ammonium acetate, pH 4 (buffer A) was applied at a flow rate of 0.8 ml/min (Shimadzu LC-30 AD pumps) as follows: 0–4 min, 0% B; 4–14 min, 0–5% B; 14–24 min, 5–15% B; 24–34 min, 15–35% B; 34–35 min, return to starting conditions . .. The RP-amide HPLC column was calibrated daily in terms of glucose units using a pyridylaminated dextran hydrolysate and the degree of polymerisation of single standards was verified by MALDI-TOF–MS .

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    Millipore trifluoroacetic acid
    Trifluoroacetic Acid, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 601 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trifluoroacetic acid/product/Millipore
    Average 99 stars, based on 601 article reviews
    Price from $9.99 to $1999.99
    trifluoroacetic acid - by Bioz Stars, 2020-07
    99/100 stars
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