invitrolon pvdf filter paper sandwich  (Thermo Fisher)


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    Structured Review

    Thermo Fisher invitrolon pvdf filter paper sandwich
    Invitrolon Pvdf Filter Paper Sandwich, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/invitrolon pvdf filter paper sandwich/product/Thermo Fisher
    Average 97 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    invitrolon pvdf filter paper sandwich - by Bioz Stars, 2020-04
    97/100 stars

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    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: MIR93 (microRNA -93) regulates tumorigenicity and therapy response of glioblastoma by targeting autophagy
    Article Snippet: .. Equal amounts of protein samples (10 μg/lane) were fractionated on SDS–polyacrylamide gel electrophoresis and transferred onto a polyvinylidene difluoride (PVDF) membrane (Thermo Fisher Scientific, LC2005) in 25 mM Tris and 192 mM glycine. .. Membranes were blocked with 5% nonfat dry milk in PBS (Thermo Fisher Scientific, AM9625), 0.05% Tween-20 (Sigma-Aldrich, P9416) for 1 h and then probed with the indicated antibodies at desired dilutions at 4°C overnight, followed by corresponding secondary antibodies (DAKO, anti-rabbit immunoglobulins, P0217, anti-mouse immunoglobulins, P0260; and anti-goat immunoglobulins, P0449).

    Centrifugation:

    Article Title: CHRFAM7A alters Binding to the Neuronal alpha-7 Nicotinic Acetylcholine Receptor
    Article Snippet: The protein was extracted after 30 minutes incubation on ice by centrifugation at 13000 rpm for 15 minuntes. .. Fifty μg of total PC12 protein extracts, and a standard molecular weight marker (New England Biolabs) were size-fractionated on 4–12% SDS-PAGE mini-gel and transferred to a PVDF membrane (Invitrogen, #LC2005).

    Protease Inhibitor:

    Article Title: MIR93 (microRNA -93) regulates tumorigenicity and therapy response of glioblastoma by targeting autophagy
    Article Snippet: The cell lysates were prepared in RIPA buffer (50 mM Tris-HCl, pH 8.0 [Thermo Fisher Scientific, 15568-025], 150 mM sodium chloride [Sigma-Aldrich, S9888], 1% NP-40 [Sigma-Aldrich, I3021, 0.5% sodium deoxycholate [Sigma-Aldrich, D6750], 0.1% sodium dodecyl sulfate [Wako, 191-07145], 2 mM EDTA [Dojindo, 345-01865]) containing 1 X protease inhibitor cocktail (Nacalai Tesque, 25955-11). .. Equal amounts of protein samples (10 μg/lane) were fractionated on SDS–polyacrylamide gel electrophoresis and transferred onto a polyvinylidene difluoride (PVDF) membrane (Thermo Fisher Scientific, LC2005) in 25 mM Tris and 192 mM glycine.

    Transfection:

    Article Title: CHRFAM7A alters Binding to the Neuronal alpha-7 Nicotinic Acetylcholine Receptor
    Article Snippet: Fifty μg of total PC12 protein extracts, and a standard molecular weight marker (New England Biolabs) were size-fractionated on 4–12% SDS-PAGE mini-gel and transferred to a PVDF membrane (Invitrogen, #LC2005). .. Specificity of the antibody for CHRFAM7A and its inability to cross-react with the α7nAchR was established by immunoblotting transfected cells, respectively.

    Blocking Assay:

    Article Title: CHRFAM7A alters Binding to the Neuronal alpha-7 Nicotinic Acetylcholine Receptor
    Article Snippet: Fifty μg of total PC12 protein extracts, and a standard molecular weight marker (New England Biolabs) were size-fractionated on 4–12% SDS-PAGE mini-gel and transferred to a PVDF membrane (Invitrogen, #LC2005). .. The membranes were incubated for 1 hour with blocking buffer (5% BSA in TBS with 0.1% Tween - TBST), following incubation with anti-CHRFAM7A (1μg/ml).

    Enzyme-linked Immunosorbent Assay:

    Article Title: CHRFAM7A alters Binding to the Neuronal alpha-7 Nicotinic Acetylcholine Receptor
    Article Snippet: Fifty μg of total PC12 protein extracts, and a standard molecular weight marker (New England Biolabs) were size-fractionated on 4–12% SDS-PAGE mini-gel and transferred to a PVDF membrane (Invitrogen, #LC2005). .. For creation of a specific antibody for CHRFAM7A, antibodies to the 27 amino acid peptide that differentiates CHRFAM7A from CHRNA7 were raised in rabbits by contract with GenWay (San Diego, CA), affinity purified by the supplier, and titers measured by ELISA with the synthetic peptide.

    Incubation:

    Article Title: CHRFAM7A alters Binding to the Neuronal alpha-7 Nicotinic Acetylcholine Receptor
    Article Snippet: The protein was extracted after 30 minutes incubation on ice by centrifugation at 13000 rpm for 15 minuntes. .. Fifty μg of total PC12 protein extracts, and a standard molecular weight marker (New England Biolabs) were size-fractionated on 4–12% SDS-PAGE mini-gel and transferred to a PVDF membrane (Invitrogen, #LC2005).

    BIA-KA:

    Article Title: CHRFAM7A alters Binding to the Neuronal alpha-7 Nicotinic Acetylcholine Receptor
    Article Snippet: Cell protein concentrations were measured using the BCA method. .. Fifty μg of total PC12 protein extracts, and a standard molecular weight marker (New England Biolabs) were size-fractionated on 4–12% SDS-PAGE mini-gel and transferred to a PVDF membrane (Invitrogen, #LC2005).

    Marker:

    Article Title: CHRFAM7A alters Binding to the Neuronal alpha-7 Nicotinic Acetylcholine Receptor
    Article Snippet: .. Fifty μg of total PC12 protein extracts, and a standard molecular weight marker (New England Biolabs) were size-fractionated on 4–12% SDS-PAGE mini-gel and transferred to a PVDF membrane (Invitrogen, #LC2005). .. The membranes were incubated for 1 hour with blocking buffer (5% BSA in TBS with 0.1% Tween - TBST), following incubation with anti-CHRFAM7A (1μg/ml).

    Sonication:

    Article Title: CHRFAM7A alters Binding to the Neuronal alpha-7 Nicotinic Acetylcholine Receptor
    Article Snippet: The pellets were resuspended in 300 μl of Triton X-100-containing buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, protease and phosphatase inhibitors (Sigma-Aldrich Co.), 5 mM DTT and 0.2 mM PMSF) and sonicated at moderate speed on ice (three times for 20 seconds with 30 second intervals). .. Fifty μg of total PC12 protein extracts, and a standard molecular weight marker (New England Biolabs) were size-fractionated on 4–12% SDS-PAGE mini-gel and transferred to a PVDF membrane (Invitrogen, #LC2005).

    Affinity Purification:

    Article Title: CHRFAM7A alters Binding to the Neuronal alpha-7 Nicotinic Acetylcholine Receptor
    Article Snippet: Fifty μg of total PC12 protein extracts, and a standard molecular weight marker (New England Biolabs) were size-fractionated on 4–12% SDS-PAGE mini-gel and transferred to a PVDF membrane (Invitrogen, #LC2005). .. For creation of a specific antibody for CHRFAM7A, antibodies to the 27 amino acid peptide that differentiates CHRFAM7A from CHRNA7 were raised in rabbits by contract with GenWay (San Diego, CA), affinity purified by the supplier, and titers measured by ELISA with the synthetic peptide.

    SDS Page:

    Article Title: CHRFAM7A alters Binding to the Neuronal alpha-7 Nicotinic Acetylcholine Receptor
    Article Snippet: .. Fifty μg of total PC12 protein extracts, and a standard molecular weight marker (New England Biolabs) were size-fractionated on 4–12% SDS-PAGE mini-gel and transferred to a PVDF membrane (Invitrogen, #LC2005). .. The membranes were incubated for 1 hour with blocking buffer (5% BSA in TBS with 0.1% Tween - TBST), following incubation with anti-CHRFAM7A (1μg/ml).

    Molecular Weight:

    Article Title: CHRFAM7A alters Binding to the Neuronal alpha-7 Nicotinic Acetylcholine Receptor
    Article Snippet: .. Fifty μg of total PC12 protein extracts, and a standard molecular weight marker (New England Biolabs) were size-fractionated on 4–12% SDS-PAGE mini-gel and transferred to a PVDF membrane (Invitrogen, #LC2005). .. The membranes were incubated for 1 hour with blocking buffer (5% BSA in TBS with 0.1% Tween - TBST), following incubation with anti-CHRFAM7A (1μg/ml).

    Software:

    Article Title: MIR93 (microRNA -93) regulates tumorigenicity and therapy response of glioblastoma by targeting autophagy
    Article Snippet: Equal amounts of protein samples (10 μg/lane) were fractionated on SDS–polyacrylamide gel electrophoresis and transferred onto a polyvinylidene difluoride (PVDF) membrane (Thermo Fisher Scientific, LC2005) in 25 mM Tris and 192 mM glycine. .. The results of IB analyses were quantified by using ImageJ software.

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  • 99
    Thermo Fisher invitrolon pvdf filter paper sandwich
    Invitrolon Pvdf Filter Paper Sandwich, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/invitrolon pvdf filter paper sandwich/product/Thermo Fisher
    Average 99 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    invitrolon pvdf filter paper sandwich - by Bioz Stars, 2020-04
    99/100 stars
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