intron 27  (Addgene inc)

 
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  • 95
    Name:
    pCA G intron G Plasmid 40027
    Description:
    Standard format Plasmid sent in bacteria as agar stab
    Catalog Number:
    40027
    Price:
    None
    Category:
    Plasmid
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    Structured Review

    Addgene inc intron 27
    pCA G intron G Plasmid 40027
    Standard format Plasmid sent in bacteria as agar stab
    https://www.bioz.com/result/intron 27/product/Addgene inc
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    intron 27 - by Bioz Stars, 2021-07
    95/100 stars

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    Related Articles

    Sequencing:

    Article Title: ASH2L drives proliferation and sensitivity to bleomycin and other genotoxins in Hodgkin’s lymphoma and testicular cancer cells
    Article Snippet: .. Sequencing this plasmid revealed the presence of 82 base pairs of intron 27 before the last exon of the codon sequence. ..

    Article Title: Comparative analyses of gene expression in common marmoset and human pluripotent stem cells (PSCs) identify factors enhancing homologous recombination efficiency in the HPRT locus of human PSCs
    Article Snippet: 2.2 Vector construction The human HPRT targeting vector HPRT-TV was constructed using the GKI-2.0 system ( ). .. In brief, based on sequence information of the human X chromosome from the NCBI genome browser (GRCh38.p13, Chromosome X - NC_000023.11: 134460165..134500668), the 5’ homology arm (3.0 kb) and 3’ homology arm (12.8 kb), which are around the 2nd intron of the gene, were subcloned respectively into pDONR-P3P1r (Addgene #141014) and pDONR-P2rP4 (Addgene #141015) using the Gateway cloning system (BP reaction). .. The targeting vector containing a PGK-Neo drug-resistance cassette flanked by the HPRT homology arms was constructed by Gateway LR reaction using pENTR-L1-PGK-Neo-L2 (Addgene #141007), pUC-DEST-R3R4(R) (Addgene #141010) and the Gateway LR Clonase II Enzyme Mix (Thermo Fisher Scientific).

    Article Title: G-quadruplexes offer a conserved structural motif for NONO recruitment to NEAT1 architectural lncRNA
    Article Snippet: The pCDNA3.0-eG-FP-tRSA and pCDNA3.0-eG-FP-tRSA-(GGGGCC)70 plasmids were produced as follows. .. The pcDNA3.0 backbone was linearized with XbaI and BamHI and a GFP-coding sequence containing an intron (‘eG-FP’), from pCA-G-intron-G ( ) (gift from Liqun Luo, Addgene # 40027), and a 3′ EcoRI site was inserted using Gibson assembly to yield pCDNA3.0-eG-FP. .. A tRNA scaffolded SA ('tRSA') sequence was amplified from pcDNA3-tRSA ( ) (gift from Ian Macara, Addgene # 32200) and ligated into the EcoRI site of pCDNA3.0-eG-FP to produce pcDNA3.0-eG-FP-tRSA. (GGGGCC)70 sequence flanked by EcoRI sites was cut from pCR8-70 ( ) and inserted into the EcoRI site of pcDNA3.0-eG-FP-tRSA to produce pCDNA3.0-eG-FP-tRSA-(GGGGCC)70 .

    Plasmid Preparation:

    Article Title: ASH2L drives proliferation and sensitivity to bleomycin and other genotoxins in Hodgkin’s lymphoma and testicular cancer cells
    Article Snippet: .. Sequencing this plasmid revealed the presence of 82 base pairs of intron 27 before the last exon of the codon sequence. ..

    Clone Assay:

    Article Title: Comparative analyses of gene expression in common marmoset and human pluripotent stem cells (PSCs) identify factors enhancing homologous recombination efficiency in the HPRT locus of human PSCs
    Article Snippet: 2.2 Vector construction The human HPRT targeting vector HPRT-TV was constructed using the GKI-2.0 system ( ). .. In brief, based on sequence information of the human X chromosome from the NCBI genome browser (GRCh38.p13, Chromosome X - NC_000023.11: 134460165..134500668), the 5’ homology arm (3.0 kb) and 3’ homology arm (12.8 kb), which are around the 2nd intron of the gene, were subcloned respectively into pDONR-P3P1r (Addgene #141014) and pDONR-P2rP4 (Addgene #141015) using the Gateway cloning system (BP reaction). .. The targeting vector containing a PGK-Neo drug-resistance cassette flanked by the HPRT homology arms was constructed by Gateway LR reaction using pENTR-L1-PGK-Neo-L2 (Addgene #141007), pUC-DEST-R3R4(R) (Addgene #141010) and the Gateway LR Clonase II Enzyme Mix (Thermo Fisher Scientific).

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  • 95
    Addgene inc intron 27
    Intron 27, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/intron 27/product/Addgene inc
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    intron 27 - by Bioz Stars, 2021-07
    95/100 stars
      Buy from Supplier

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    Standard format Plasmid sent in bacteria as agar stab
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