internal control glyceraldehyde 3 phosphate dehydrogenase gapdh  (Millipore)


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    Structured Review

    Millipore internal control glyceraldehyde 3 phosphate dehydrogenase gapdh
    Internal Control Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/internal control glyceraldehyde 3 phosphate dehydrogenase gapdh/product/Millipore
    Average 95 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    internal control glyceraldehyde 3 phosphate dehydrogenase gapdh - by Bioz Stars, 2020-04
    95/100 stars

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    Incubation:

    Article Title: KMUP-1, a GPCR Modulator, Attenuates Triglyceride Accumulation Involved MAPKs/Akt/PPARγ and PKA/PKG/HSL Signaling in 3T3-L1 Preadipocytes
    Article Snippet: The blot was then incubated with anti-mouse or anti–goat IgG linked to alkaline phosphatase (1:1000) for 1 h. Immunoreactive bands were visualized using horseradish peroxidase–conjugated secondary antibodies with subsequent enhanced chemiluminescence (ECL) detection (GE Healthcare Bio-Sciences Corp., Piscataway, NJ, USA). .. Mouse or rabbit primary antibodies of p-ERK, p-p38, p-JNK, p-Akt, p-HSL (1:1000 dilution; Cell Signaling, Boston, MA, USA), PPARγ (1:1000 dilution; Santa Cruz Biotechnology, Santa Cruz, CA, USA), PKA, PKG (1:1000 dilution; Abcam, London, UK), and internal control glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:2000 dilution; Millipore, Temecula, CA, USA) were used in Western blot analyses.

    Blocking Assay:

    Article Title: KMUP-1, a GPCR Modulator, Attenuates Triglyceride Accumulation Involved MAPKs/Akt/PPARγ and PKA/PKG/HSL Signaling in 3T3-L1 Preadipocytes
    Article Snippet: Separated proteins were transferred to polyvinylidene fluoride (PVDF) membranes treated with 5% fat-free milk powder to block nonspecific IgGs (90 min, 100 V) and incubated for 1 h with specific protein antibody. .. Mouse or rabbit primary antibodies of p-ERK, p-p38, p-JNK, p-Akt, p-HSL (1:1000 dilution; Cell Signaling, Boston, MA, USA), PPARγ (1:1000 dilution; Santa Cruz Biotechnology, Santa Cruz, CA, USA), PKA, PKG (1:1000 dilution; Abcam, London, UK), and internal control glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:2000 dilution; Millipore, Temecula, CA, USA) were used in Western blot analyses.

    Electrophoresis:

    Article Title: KMUP-1, a GPCR Modulator, Attenuates Triglyceride Accumulation Involved MAPKs/Akt/PPARγ and PKA/PKG/HSL Signaling in 3T3-L1 Preadipocytes
    Article Snippet: Electrophoresis was performed using 10% SDS-polyacrylamide gel (1 h, 100 V, 40 mA, 20 µg protein). .. Mouse or rabbit primary antibodies of p-ERK, p-p38, p-JNK, p-Akt, p-HSL (1:1000 dilution; Cell Signaling, Boston, MA, USA), PPARγ (1:1000 dilution; Santa Cruz Biotechnology, Santa Cruz, CA, USA), PKA, PKG (1:1000 dilution; Abcam, London, UK), and internal control glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:2000 dilution; Millipore, Temecula, CA, USA) were used in Western blot analyses.

    Protein Extraction:

    Article Title: KMUP-1, a GPCR Modulator, Attenuates Triglyceride Accumulation Involved MAPKs/Akt/PPARγ and PKA/PKG/HSL Signaling in 3T3-L1 Preadipocytes
    Article Snippet: Western Blotting Analysis After 5 days of culture, 3T3-L1 cells were placed in an extraction buffer (Tris 10 mM, pH 7.0, NaCl 140 mM, PMSF 2 mM, DTT 5 mM, NP-40 0.5%, pepstatin A 0.05 mM, and leupeptin 0.2 mM) for protein extraction, and centrifuged at 12,500 g for 30 min. To measure protein expression levels, the total cell proteins were extracted after incubation with KMUP-1 (1, 10, 20, or 40 µM) and then Western blotting analyses were performed as described previously [ , ]. .. Mouse or rabbit primary antibodies of p-ERK, p-p38, p-JNK, p-Akt, p-HSL (1:1000 dilution; Cell Signaling, Boston, MA, USA), PPARγ (1:1000 dilution; Santa Cruz Biotechnology, Santa Cruz, CA, USA), PKA, PKG (1:1000 dilution; Abcam, London, UK), and internal control glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:2000 dilution; Millipore, Temecula, CA, USA) were used in Western blot analyses.

    Expressing:

    Article Title: KMUP-1, a GPCR Modulator, Attenuates Triglyceride Accumulation Involved MAPKs/Akt/PPARγ and PKA/PKG/HSL Signaling in 3T3-L1 Preadipocytes
    Article Snippet: Western Blotting Analysis After 5 days of culture, 3T3-L1 cells were placed in an extraction buffer (Tris 10 mM, pH 7.0, NaCl 140 mM, PMSF 2 mM, DTT 5 mM, NP-40 0.5%, pepstatin A 0.05 mM, and leupeptin 0.2 mM) for protein extraction, and centrifuged at 12,500 g for 30 min. To measure protein expression levels, the total cell proteins were extracted after incubation with KMUP-1 (1, 10, 20, or 40 µM) and then Western blotting analyses were performed as described previously [ , ]. .. Mouse or rabbit primary antibodies of p-ERK, p-p38, p-JNK, p-Akt, p-HSL (1:1000 dilution; Cell Signaling, Boston, MA, USA), PPARγ (1:1000 dilution; Santa Cruz Biotechnology, Santa Cruz, CA, USA), PKA, PKG (1:1000 dilution; Abcam, London, UK), and internal control glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:2000 dilution; Millipore, Temecula, CA, USA) were used in Western blot analyses.

    Western Blot:

    Article Title: KMUP-1, a GPCR Modulator, Attenuates Triglyceride Accumulation Involved MAPKs/Akt/PPARγ and PKA/PKG/HSL Signaling in 3T3-L1 Preadipocytes
    Article Snippet: .. Mouse or rabbit primary antibodies of p-ERK, p-p38, p-JNK, p-Akt, p-HSL (1:1000 dilution; Cell Signaling, Boston, MA, USA), PPARγ (1:1000 dilution; Santa Cruz Biotechnology, Santa Cruz, CA, USA), PKA, PKG (1:1000 dilution; Abcam, London, UK), and internal control glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:2000 dilution; Millipore, Temecula, CA, USA) were used in Western blot analyses. .. Real-Time Quantitative Polymerase Chain Reaction (qPCR) mRNAs of PPARγ1 and PPARγ2 were evaluated by the qPCR method in incubation with KMUP-1 (1, 10, 20, and 40 µM) over different time periods.

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  • 95
    Millipore internal control glyceraldehyde 3 phosphate dehydrogenase gapdh
    Internal Control Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/internal control glyceraldehyde 3 phosphate dehydrogenase gapdh/product/Millipore
    Average 95 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    internal control glyceraldehyde 3 phosphate dehydrogenase gapdh - by Bioz Stars, 2020-04
    95/100 stars
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