insulin  (Millipore)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97
    Name:
    Sigma Aldrich power supply
    Description:

    Catalog Number:
    ep2001
    Price:
    None
    Buy from Supplier


    Structured Review

    Millipore insulin

    https://www.bioz.com/result/insulin/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    insulin - by Bioz Stars, 2021-03
    97/100 stars

    Images

    Related Articles

    Translocation Assay:

    Article Title: Non-Apoptotic Toxicity of Pseudomonas aeruginosa toward Murine Cells
    Article Snippet: The slides were viewed at 100× magnification under oil immersion in an epifluorescence microscope and by Deltavision microscopy. .. p65 Translocation Assays For indirect immunofluorescence analysis, 5×103 cells were grown on 18 mm glass coverslips and exposed to either PAO1, MOI = 10 or to 100 µg/ml LPS of P. aeruginosa , serotype 10 (Sigma-Aldrich L-9143), for 1 h, washed, and recultured as required. .. Cells were fixed with 4% paraformaldehyde in PBS for 15 min at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 1 min at room temperature and stained with rabbit anti-p65 (1∶100; eBioscience Ltd) in PBS containing 10% goat serum for 30 min at room temperature.

    Immunofluorescence:

    Article Title: Non-Apoptotic Toxicity of Pseudomonas aeruginosa toward Murine Cells
    Article Snippet: The slides were viewed at 100× magnification under oil immersion in an epifluorescence microscope and by Deltavision microscopy. .. p65 Translocation Assays For indirect immunofluorescence analysis, 5×103 cells were grown on 18 mm glass coverslips and exposed to either PAO1, MOI = 10 or to 100 µg/ml LPS of P. aeruginosa , serotype 10 (Sigma-Aldrich L-9143), for 1 h, washed, and recultured as required. .. Cells were fixed with 4% paraformaldehyde in PBS for 15 min at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 1 min at room temperature and stained with rabbit anti-p65 (1∶100; eBioscience Ltd) in PBS containing 10% goat serum for 30 min at room temperature.

    Transfection:

    Article Title: Caspase-1 cleaves Bid to release mitochondrial SMAC and drive secondary necrosis in the absence of GSDMD
    Article Snippet: Similarly, the cells were primed with 100 ng/ml LPS LPS-B5 (055:B5; InvivoGen) for 4 h in OptiMEM. .. LPS/FuGeneHD complexes were prepared by mixing 100 μl OptiMEM with 2 μg ultrapure LPS O111:B4 (InvivoGen) and 0.5 μl of FuGENE HD (Sigma-Aldrich) per well to be transfected. .. The transfection mixture was vortexed briefly, incubated for 10 min at room temperature, and added dropwise to the cells.

    Isolation:

    Article Title: Paternal sepsis induces alterations of the sperm methylome and dampens offspring immune responses—an animal study
    Article Snippet: For the isolation and analysis of sperm, a separate cohort of animals, both CLP as well as sham, was operated. .. Sperm isolation and analysis Mature sperm cells were isolated from the Cauda epididymis of 9 CLP and 7 sham mice into Donners medium (135 mM NaCl (Sigma-Aldrich, Steinheim, Germany), 5 mM KCl (Merck, Darmstadt, Germany), 1 mM MgSO4 (Sigma-Aldrich, Steinheim, Germany), 2 mM CaCl2 (Sigma-Aldrich, Steinheim, Germany), 30 mM HEPES pH 7.4 (Roth, Karlsruhe, Germany); freshly supplemented with 0.53% sodium lactate (Caelo, Hilden, Germany), 1 mM sodium pyruvate (Life Technologies, Darmstadt, Germany), 20 mg/mL BSA (Roth, Karlsruhe, Germany) and 25 mM NaHCO3 (Roth, Karlsruhe, Germany)) at 37 °C and 5% CO2 for 40 min via swim-up assay. ..

    Mouse Assay:

    Article Title: Paternal sepsis induces alterations of the sperm methylome and dampens offspring immune responses—an animal study
    Article Snippet: For the isolation and analysis of sperm, a separate cohort of animals, both CLP as well as sham, was operated. .. Sperm isolation and analysis Mature sperm cells were isolated from the Cauda epididymis of 9 CLP and 7 sham mice into Donners medium (135 mM NaCl (Sigma-Aldrich, Steinheim, Germany), 5 mM KCl (Merck, Darmstadt, Germany), 1 mM MgSO4 (Sigma-Aldrich, Steinheim, Germany), 2 mM CaCl2 (Sigma-Aldrich, Steinheim, Germany), 30 mM HEPES pH 7.4 (Roth, Karlsruhe, Germany); freshly supplemented with 0.53% sodium lactate (Caelo, Hilden, Germany), 1 mM sodium pyruvate (Life Technologies, Darmstadt, Germany), 20 mg/mL BSA (Roth, Karlsruhe, Germany) and 25 mM NaHCO3 (Roth, Karlsruhe, Germany)) at 37 °C and 5% CO2 for 40 min via swim-up assay. ..

    other:

    Article Title: The Antiproliferative Effect of Cyclodipeptides from Pseudomonas aeruginosa PAO1 on HeLa Cells Involves Inhibition of Phosphorylation of Akt and S6k Kinases
    Article Snippet: Antibiotic concentrations used for the P. aeruginosa were 200 μg/mL streptomycin; all reagents were purchased from Sigma-Aldrich Co.

    Article Title: Polyurethane/Nanosilver-Doped Halloysite Nanocomposites: Thermal, Mechanical Properties, and Antibacterial Properties
    Article Snippet: PTMG (Mw = 1000) was obtained from Sigma-Aldrich.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97
    Millipore pro insulin c peptide mouse monoclonal antibody
    In vitro  differentiation of mESCs into pancreatic islet-like endocrine cells (PILECs). (A) Phase contrast images of wild-type (WT) mESCs and menin-null (KO) mESCs differentiated into PILECs. (B) RT-PCR analysis measuring mRNA levels of genes expressed in islet cells using RNA from mESCs and mESC-derived PILECs. WT: wild-type cells; KO:  Men1 -ko cells (menin-null). RNA from the mouse insulinoma cell line MIN6 was used as a positive control. Gapdh served as an internal control for RT-PCR using a 1∶10 dilution of the oligo-dT primed first strand cDNA template. (C) Western blot analysis of whole cell protein extracts from wt or menin-null mESCs before and after differentiation into PILECs with antibodies against menin, an ESC pluripotency marker Oct3/4, and an islet differentiation marker NeuroD1. Tubulin served as protein loading control. (D)  In vitro  differentiation of pancreatic precursor cells (step-3) derived from mESCs into PILECS was performed in gelatinized chamber slides and processed for immunofluorescence (red) staining with a pro-insulin C-peptide mouse monoclonal antibody to detect insulin. MIN6 cells cultured in chamber slides were used as a positive control.
    Pro Insulin C Peptide Mouse Monoclonal Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pro insulin c peptide mouse monoclonal antibody/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pro insulin c peptide mouse monoclonal antibody - by Bioz Stars, 2021-03
    97/100 stars
      Buy from Supplier

    94
    Millipore insulin radioimmunoassay
    Serum insulin levels and gene expression are decreased with leptin treatment. A. Sera from BBDR rats in the AdLeptin + pIC+KRV, AdBetagal + pIC+KRV, PBS + pIC+KRV or PBS only groups were analyzed for insulin levels by <t>radioimmunoassay</t> on day 0 (day of
    Insulin Radioimmunoassay, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/insulin radioimmunoassay/product/Millipore
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    insulin radioimmunoassay - by Bioz Stars, 2021-03
    94/100 stars
      Buy from Supplier

    93
    Millipore mouse rat insulin elisa kit
    Circulating levels of <t>IGF-1</t> and FFAs from control, control + Hex, STZ, and STZ + Hex rats. After the STZ or Hex treatment, terminal blood samples were collected. Circulating levels of ( A ) IGF-1 and ( B ) FFAs relative to fat tissue were determined by IGF-1 and FFAs <t>ELISA</t> kits. n = 6 from each group, data are shown as the mean ± SEM. *** p
    Mouse Rat Insulin Elisa Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse rat insulin elisa kit/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse rat insulin elisa kit - by Bioz Stars, 2021-03
    93/100 stars
      Buy from Supplier

    99
    Millipore glycation end
    Level of advanced <t>glycation</t> end product in serum of control, diabetic, and ginseng extract-fed diabetic groups. Results were expressed as the mean ± standard error; * P
    Glycation End, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glycation end/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    glycation end - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

    Image Search Results


    In vitro  differentiation of mESCs into pancreatic islet-like endocrine cells (PILECs). (A) Phase contrast images of wild-type (WT) mESCs and menin-null (KO) mESCs differentiated into PILECs. (B) RT-PCR analysis measuring mRNA levels of genes expressed in islet cells using RNA from mESCs and mESC-derived PILECs. WT: wild-type cells; KO:  Men1 -ko cells (menin-null). RNA from the mouse insulinoma cell line MIN6 was used as a positive control. Gapdh served as an internal control for RT-PCR using a 1∶10 dilution of the oligo-dT primed first strand cDNA template. (C) Western blot analysis of whole cell protein extracts from wt or menin-null mESCs before and after differentiation into PILECs with antibodies against menin, an ESC pluripotency marker Oct3/4, and an islet differentiation marker NeuroD1. Tubulin served as protein loading control. (D)  In vitro  differentiation of pancreatic precursor cells (step-3) derived from mESCs into PILECS was performed in gelatinized chamber slides and processed for immunofluorescence (red) staining with a pro-insulin C-peptide mouse monoclonal antibody to detect insulin. MIN6 cells cultured in chamber slides were used as a positive control.

    Journal: PLoS ONE

    Article Title: Genome-Wide Characterization of Menin-Dependent H3K4me3 Reveals a Specific Role for Menin in the Regulation of Genes Implicated in MEN1-Like Tumors

    doi: 10.1371/journal.pone.0037952

    Figure Lengend Snippet: In vitro differentiation of mESCs into pancreatic islet-like endocrine cells (PILECs). (A) Phase contrast images of wild-type (WT) mESCs and menin-null (KO) mESCs differentiated into PILECs. (B) RT-PCR analysis measuring mRNA levels of genes expressed in islet cells using RNA from mESCs and mESC-derived PILECs. WT: wild-type cells; KO: Men1 -ko cells (menin-null). RNA from the mouse insulinoma cell line MIN6 was used as a positive control. Gapdh served as an internal control for RT-PCR using a 1∶10 dilution of the oligo-dT primed first strand cDNA template. (C) Western blot analysis of whole cell protein extracts from wt or menin-null mESCs before and after differentiation into PILECs with antibodies against menin, an ESC pluripotency marker Oct3/4, and an islet differentiation marker NeuroD1. Tubulin served as protein loading control. (D) In vitro differentiation of pancreatic precursor cells (step-3) derived from mESCs into PILECS was performed in gelatinized chamber slides and processed for immunofluorescence (red) staining with a pro-insulin C-peptide mouse monoclonal antibody to detect insulin. MIN6 cells cultured in chamber slides were used as a positive control.

    Article Snippet: Immunofluorescence mESCs differentiated into PILECs in gelatinized chamber slides were fixed in 4% paraformaldehyde, permeabilized with 0.5% Triton X-100, and stained with pro-insulin C-peptide mouse monoclonal antibody (Millipore, AB1342) and anti-mouse secondary antibody conjugated to Texas Red (Jackson ImmunoResearch Laboratories).

    Techniques: In Vitro, Reverse Transcription Polymerase Chain Reaction, Derivative Assay, Positive Control, Western Blot, Marker, Immunofluorescence, Staining, Cell Culture

    Serum insulin levels and gene expression are decreased with leptin treatment. A. Sera from BBDR rats in the AdLeptin + pIC+KRV, AdBetagal + pIC+KRV, PBS + pIC+KRV or PBS only groups were analyzed for insulin levels by radioimmunoassay on day 0 (day of

    Journal: Autoimmunity

    Article Title: Leptin treatment confers clinical benefit at multiple stages of virally induced type 1 diabetes in BB rats

    doi: 10.3109/08916934.2010.482116

    Figure Lengend Snippet: Serum insulin levels and gene expression are decreased with leptin treatment. A. Sera from BBDR rats in the AdLeptin + pIC+KRV, AdBetagal + pIC+KRV, PBS + pIC+KRV or PBS only groups were analyzed for insulin levels by radioimmunoassay on day 0 (day of

    Article Snippet: Insulin radioimmunoassay was performed by Millipore (Billerica, MA).

    Techniques: Expressing, RIA Assay

    Circulating levels of IGF-1 and FFAs from control, control + Hex, STZ, and STZ + Hex rats. After the STZ or Hex treatment, terminal blood samples were collected. Circulating levels of ( A ) IGF-1 and ( B ) FFAs relative to fat tissue were determined by IGF-1 and FFAs ELISA kits. n = 6 from each group, data are shown as the mean ± SEM. *** p

    Journal: International Journal of Molecular Sciences

    Article Title: Enhanced Pulsatile Growth Hormone Secretion and Altered Metabolic Hormones by in Vivo Hexarelin Treatment in Streptozotocin-Induced Diabetic Rats

    doi: 10.3390/ijms19103067

    Figure Lengend Snippet: Circulating levels of IGF-1 and FFAs from control, control + Hex, STZ, and STZ + Hex rats. After the STZ or Hex treatment, terminal blood samples were collected. Circulating levels of ( A ) IGF-1 and ( B ) FFAs relative to fat tissue were determined by IGF-1 and FFAs ELISA kits. n = 6 from each group, data are shown as the mean ± SEM. *** p

    Article Snippet: Circulating levels of IGF-1, insulin, and FFAs were determined by Mouse/Rat IGF-1 Quantikine ELISA kit (R & D Systems, Minneapolis, MN, USA), Mouse/Rat Insulin ELISA kit (EMD Millipore, St. Charles, MI, USA), and nonesterified fatty acids (NEFA-C) Assay (Wako, Osaka, Japan), respectively.

    Techniques: Enzyme-linked Immunosorbent Assay

    Level of advanced glycation end product in serum of control, diabetic, and ginseng extract-fed diabetic groups. Results were expressed as the mean ± standard error; * P

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Ginseng Extracts Restore High-Glucose Induced Vascular Dysfunctions by Altering Triglyceride Metabolism and Downregulation of Atherosclerosis-Related Genes

    doi: 10.1155/2013/797310

    Figure Lengend Snippet: Level of advanced glycation end product in serum of control, diabetic, and ginseng extract-fed diabetic groups. Results were expressed as the mean ± standard error; * P

    Article Snippet: Serum insulin and glycation end products were measured using kits purchased from Millipore (EZRMI-13K) and Cusabio (CSB-E08140r), respectively.

    Techniques: