instaclone pcr cloning strategy  (Thermo Fisher)


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    Name:
    InsTAclone PCR Cloning Kit
    Description:

    Catalog Number:
    k1213
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    Structured Review

    Thermo Fisher instaclone pcr cloning strategy

    https://www.bioz.com/result/instaclone pcr cloning strategy/product/Thermo Fisher
    Average 98 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    instaclone pcr cloning strategy - by Bioz Stars, 2020-07
    98/100 stars

    Related Products / Commonly Used Together

    pcr fragments
    ptz57r/t

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    Related Articles

    Clone Assay:

    Article Title: Bacterial contaminants from frozen puff pastry production process and their growth inhibition by antimicrobial substances from lactic acid bacteria. Bacterial contaminants from frozen puff pastry production process and their growth inhibition by antimicrobial substances from lactic acid bacteria
    Article Snippet: .. The obtained PCR products were purified using a B.E.Z.N.A.™ gel extraction kit (Omega Bio‐Tek, Norcross, GA, USA), and further cloned into pTZ57R/T vector (InsTAclone™ PCR cloning Kit, Fermentas, Waltham, Massachusetts, USA) using the E. coli strain DH5α as a host cell, and screened using blue/white colonies. .. The recombinant plasmids were purified using QIAprep® Spin miniprep kit (QIAGEN, Hilden, Germany), and sequenced by the 1st Based Company (Malaysia).

    Article Title: Cloning, Expression, and Purification of Hyperthermophile α-Amylase from Pyrococcus woesei
    Article Snippet: .. The amplified gene was cloned into pTZ57R/T (InsTAclone PCR Cloning Kit; Fermentas), and then subcloned into pTYB2 and transformed into E. coli BL21 (DE3) pLysS cells. .. 2.2 Purification of α-amylase Isolation and purification of α-amylase was undertaken as described previously .

    Article Title: Fungal Systematics and Evolution: FUSE 5
    Article Snippet: .. Both amplicons were cloned into a vector pTZ57R/T using the InsTAclone PCR Cloning Kit (ThermoFisher Scientific, Mumbai, India) and subsequently sequenced. .. Generated sequences were submitted to NCBI GenBank under accession numbers (SSU) and (ITS).

    Article Title: Aquaporin 5 Expression in Mouse Mammary Gland Cells Is Not Driven by Promoter Methylation
    Article Snippet: .. Plasmids were prepared from AQP5 and GAPDH PCR products, cloned with the InsTAclone PCR Cloning Kit (Fermentas), and purified with the GenElute Plasmid Midiprep Kit (Sigma). .. Analysis of DNA Methylation Bisulfite treatment was performed with the EZ DNA Methylation-Direct Kit (Zymo Research) on trypsinized cells according to manufacturer's instructions.

    Article Title: Extended protocol and real-time PCR dataset for shrimp species identification
    Article Snippet: .. The individual eluted DNA fragments were cloned into pTZ57R/T plasmid vector using an InsTAclone PCR Cloning Kit (Thermo Scientific, Massachusetts, USA) and subsequently transformed into E. coli XL10 Gold (Thermo Scientific, Massachusetts, USA). .. The individual plasmid DNA was extracted [ ] and sequencing of purified plasmids was performed using BigDye® Terminator v. 3.1 Cycle Sequencing Kit (Applied Biosystems, California, USA) followed by the manufacturer protocol in an automated DNA sequencing machine (Applied Biosystems, California, USA).

    Article Title: Aquaporin 5 Expression in Mouse Mammary Gland Cells Is Not Driven by Promoter Methylation
    Article Snippet: .. DNA was eluted in 30 μ L deionized H2 O and ligated into the pTZ57R/T vector using the InsTAclone PCR Cloning Kit (Fermentas), using an insert to vector ratio of 10 to 1. .. Ligation products were transformed into NEB 5alpha competent E. coli (High Efficiency) (New England Biolabs) and plated on ampicillin LB plates.

    Amplification:

    Article Title: Cloning, Expression, and Purification of Hyperthermophile α-Amylase from Pyrococcus woesei
    Article Snippet: .. The amplified gene was cloned into pTZ57R/T (InsTAclone PCR Cloning Kit; Fermentas), and then subcloned into pTYB2 and transformed into E. coli BL21 (DE3) pLysS cells. .. 2.2 Purification of α-amylase Isolation and purification of α-amylase was undertaken as described previously .

    Purification:

    Article Title: Bacterial contaminants from frozen puff pastry production process and their growth inhibition by antimicrobial substances from lactic acid bacteria. Bacterial contaminants from frozen puff pastry production process and their growth inhibition by antimicrobial substances from lactic acid bacteria
    Article Snippet: .. The obtained PCR products were purified using a B.E.Z.N.A.™ gel extraction kit (Omega Bio‐Tek, Norcross, GA, USA), and further cloned into pTZ57R/T vector (InsTAclone™ PCR cloning Kit, Fermentas, Waltham, Massachusetts, USA) using the E. coli strain DH5α as a host cell, and screened using blue/white colonies. .. The recombinant plasmids were purified using QIAprep® Spin miniprep kit (QIAGEN, Hilden, Germany), and sequenced by the 1st Based Company (Malaysia).

    Article Title: Aquaporin 5 Expression in Mouse Mammary Gland Cells Is Not Driven by Promoter Methylation
    Article Snippet: .. Plasmids were prepared from AQP5 and GAPDH PCR products, cloned with the InsTAclone PCR Cloning Kit (Fermentas), and purified with the GenElute Plasmid Midiprep Kit (Sigma). .. Analysis of DNA Methylation Bisulfite treatment was performed with the EZ DNA Methylation-Direct Kit (Zymo Research) on trypsinized cells according to manufacturer's instructions.

    Polymerase Chain Reaction:

    Article Title: Bacterial contaminants from frozen puff pastry production process and their growth inhibition by antimicrobial substances from lactic acid bacteria. Bacterial contaminants from frozen puff pastry production process and their growth inhibition by antimicrobial substances from lactic acid bacteria
    Article Snippet: .. The obtained PCR products were purified using a B.E.Z.N.A.™ gel extraction kit (Omega Bio‐Tek, Norcross, GA, USA), and further cloned into pTZ57R/T vector (InsTAclone™ PCR cloning Kit, Fermentas, Waltham, Massachusetts, USA) using the E. coli strain DH5α as a host cell, and screened using blue/white colonies. .. The recombinant plasmids were purified using QIAprep® Spin miniprep kit (QIAGEN, Hilden, Germany), and sequenced by the 1st Based Company (Malaysia).

    Article Title: Cloning, Expression, and Purification of Hyperthermophile α-Amylase from Pyrococcus woesei
    Article Snippet: .. The amplified gene was cloned into pTZ57R/T (InsTAclone PCR Cloning Kit; Fermentas), and then subcloned into pTYB2 and transformed into E. coli BL21 (DE3) pLysS cells. .. 2.2 Purification of α-amylase Isolation and purification of α-amylase was undertaken as described previously .

    Article Title: Fungal Systematics and Evolution: FUSE 5
    Article Snippet: .. Both amplicons were cloned into a vector pTZ57R/T using the InsTAclone PCR Cloning Kit (ThermoFisher Scientific, Mumbai, India) and subsequently sequenced. .. Generated sequences were submitted to NCBI GenBank under accession numbers (SSU) and (ITS).

    Article Title: Aquaporin 5 Expression in Mouse Mammary Gland Cells Is Not Driven by Promoter Methylation
    Article Snippet: .. Plasmids were prepared from AQP5 and GAPDH PCR products, cloned with the InsTAclone PCR Cloning Kit (Fermentas), and purified with the GenElute Plasmid Midiprep Kit (Sigma). .. Analysis of DNA Methylation Bisulfite treatment was performed with the EZ DNA Methylation-Direct Kit (Zymo Research) on trypsinized cells according to manufacturer's instructions.

    Article Title: Extended protocol and real-time PCR dataset for shrimp species identification
    Article Snippet: .. The individual eluted DNA fragments were cloned into pTZ57R/T plasmid vector using an InsTAclone PCR Cloning Kit (Thermo Scientific, Massachusetts, USA) and subsequently transformed into E. coli XL10 Gold (Thermo Scientific, Massachusetts, USA). .. The individual plasmid DNA was extracted [ ] and sequencing of purified plasmids was performed using BigDye® Terminator v. 3.1 Cycle Sequencing Kit (Applied Biosystems, California, USA) followed by the manufacturer protocol in an automated DNA sequencing machine (Applied Biosystems, California, USA).

    Article Title: Aquaporin 5 Expression in Mouse Mammary Gland Cells Is Not Driven by Promoter Methylation
    Article Snippet: .. DNA was eluted in 30 μ L deionized H2 O and ligated into the pTZ57R/T vector using the InsTAclone PCR Cloning Kit (Fermentas), using an insert to vector ratio of 10 to 1. .. Ligation products were transformed into NEB 5alpha competent E. coli (High Efficiency) (New England Biolabs) and plated on ampicillin LB plates.

    Gel Extraction:

    Article Title: Bacterial contaminants from frozen puff pastry production process and their growth inhibition by antimicrobial substances from lactic acid bacteria. Bacterial contaminants from frozen puff pastry production process and their growth inhibition by antimicrobial substances from lactic acid bacteria
    Article Snippet: .. The obtained PCR products were purified using a B.E.Z.N.A.™ gel extraction kit (Omega Bio‐Tek, Norcross, GA, USA), and further cloned into pTZ57R/T vector (InsTAclone™ PCR cloning Kit, Fermentas, Waltham, Massachusetts, USA) using the E. coli strain DH5α as a host cell, and screened using blue/white colonies. .. The recombinant plasmids were purified using QIAprep® Spin miniprep kit (QIAGEN, Hilden, Germany), and sequenced by the 1st Based Company (Malaysia).

    Transformation Assay:

    Article Title: Cloning, Expression, and Purification of Hyperthermophile α-Amylase from Pyrococcus woesei
    Article Snippet: .. The amplified gene was cloned into pTZ57R/T (InsTAclone PCR Cloning Kit; Fermentas), and then subcloned into pTYB2 and transformed into E. coli BL21 (DE3) pLysS cells. .. 2.2 Purification of α-amylase Isolation and purification of α-amylase was undertaken as described previously .

    Article Title: Extended protocol and real-time PCR dataset for shrimp species identification
    Article Snippet: .. The individual eluted DNA fragments were cloned into pTZ57R/T plasmid vector using an InsTAclone PCR Cloning Kit (Thermo Scientific, Massachusetts, USA) and subsequently transformed into E. coli XL10 Gold (Thermo Scientific, Massachusetts, USA). .. The individual plasmid DNA was extracted [ ] and sequencing of purified plasmids was performed using BigDye® Terminator v. 3.1 Cycle Sequencing Kit (Applied Biosystems, California, USA) followed by the manufacturer protocol in an automated DNA sequencing machine (Applied Biosystems, California, USA).

    Plasmid Preparation:

    Article Title: Bacterial contaminants from frozen puff pastry production process and their growth inhibition by antimicrobial substances from lactic acid bacteria. Bacterial contaminants from frozen puff pastry production process and their growth inhibition by antimicrobial substances from lactic acid bacteria
    Article Snippet: .. The obtained PCR products were purified using a B.E.Z.N.A.™ gel extraction kit (Omega Bio‐Tek, Norcross, GA, USA), and further cloned into pTZ57R/T vector (InsTAclone™ PCR cloning Kit, Fermentas, Waltham, Massachusetts, USA) using the E. coli strain DH5α as a host cell, and screened using blue/white colonies. .. The recombinant plasmids were purified using QIAprep® Spin miniprep kit (QIAGEN, Hilden, Germany), and sequenced by the 1st Based Company (Malaysia).

    Article Title: Fungal Systematics and Evolution: FUSE 5
    Article Snippet: .. Both amplicons were cloned into a vector pTZ57R/T using the InsTAclone PCR Cloning Kit (ThermoFisher Scientific, Mumbai, India) and subsequently sequenced. .. Generated sequences were submitted to NCBI GenBank under accession numbers (SSU) and (ITS).

    Article Title: Aquaporin 5 Expression in Mouse Mammary Gland Cells Is Not Driven by Promoter Methylation
    Article Snippet: .. Plasmids were prepared from AQP5 and GAPDH PCR products, cloned with the InsTAclone PCR Cloning Kit (Fermentas), and purified with the GenElute Plasmid Midiprep Kit (Sigma). .. Analysis of DNA Methylation Bisulfite treatment was performed with the EZ DNA Methylation-Direct Kit (Zymo Research) on trypsinized cells according to manufacturer's instructions.

    Article Title: Extended protocol and real-time PCR dataset for shrimp species identification
    Article Snippet: .. The individual eluted DNA fragments were cloned into pTZ57R/T plasmid vector using an InsTAclone PCR Cloning Kit (Thermo Scientific, Massachusetts, USA) and subsequently transformed into E. coli XL10 Gold (Thermo Scientific, Massachusetts, USA). .. The individual plasmid DNA was extracted [ ] and sequencing of purified plasmids was performed using BigDye® Terminator v. 3.1 Cycle Sequencing Kit (Applied Biosystems, California, USA) followed by the manufacturer protocol in an automated DNA sequencing machine (Applied Biosystems, California, USA).

    Article Title: Aquaporin 5 Expression in Mouse Mammary Gland Cells Is Not Driven by Promoter Methylation
    Article Snippet: .. DNA was eluted in 30 μ L deionized H2 O and ligated into the pTZ57R/T vector using the InsTAclone PCR Cloning Kit (Fermentas), using an insert to vector ratio of 10 to 1. .. Ligation products were transformed into NEB 5alpha competent E. coli (High Efficiency) (New England Biolabs) and plated on ampicillin LB plates.

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  • About
  • News
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  • Contact
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  • 98
    Thermo Fisher instaclone pcr cloning strategy
    Instaclone Pcr Cloning Strategy, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/instaclone pcr cloning strategy/product/Thermo Fisher
    Average 98 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    instaclone pcr cloning strategy - by Bioz Stars, 2020-07
    98/100 stars
      Buy from Supplier

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