inhibitor s3i 201  (Millipore)

Journal: Journal of Cellular and Molecular Medicine

Article Title: Inhibition of STAT3 by S3I ‐201 suppress peritoneal fibroblast phenotype conversion and alleviate peritoneal fibrosis

doi: 10.1111/jcmm.18381

Figure Lengend Snippet: Increased expression of collagen‐I, α‐SMA and P‐STAT3 induced by TGF‐β1 was suppressed by S3I‐201, as assessed by western blot. * p <0.05; ** p <0.01.

Article Snippet: The STAT3‐specific inhibitor S3I‐201 (#SML0330) and CG (#C9394) were purchased from Sigma‐Aldrich (Darmstadt, Germany).

Techniques: Expressing, Western Blot

Journal: Journal of Cellular and Molecular Medicine

Article Title: Inhibition of STAT3 by S3I ‐201 suppress peritoneal fibroblast phenotype conversion and alleviate peritoneal fibrosis

doi: 10.1111/jcmm.18381

Figure Lengend Snippet: Effects of different concentrations of S3I‐201 on the expression of collagen‐I and α‐SMA in RPFBs. * p <0.05; ** p <0.01.

Article Snippet: The STAT3‐specific inhibitor S3I‐201 (#SML0330) and CG (#C9394) were purchased from Sigma‐Aldrich (Darmstadt, Germany).

Techniques: Expressing

Journal: Journal of Cellular and Molecular Medicine

Article Title: Inhibition of STAT3 by S3I ‐201 suppress peritoneal fibroblast phenotype conversion and alleviate peritoneal fibrosis

doi: 10.1111/jcmm.18381

Figure Lengend Snippet: Effects of different concentrations of S3I‐201 on the expression of collagen‐I and α‐SMA in RPFBs. * p <0.05; ** p <0.01.

Article Snippet: The STAT3‐specific inhibitor S3I‐201 (#SML0330) and CG (#C9394) were purchased from Sigma‐Aldrich (Darmstadt, Germany).

Techniques: Expressing

Journal: Journal of Cellular and Molecular Medicine

Article Title: Inhibition of STAT3 by S3I ‐201 suppress peritoneal fibroblast phenotype conversion and alleviate peritoneal fibrosis

doi: 10.1111/jcmm.18381

Figure Lengend Snippet: S3I‐201 administration significantly induced reduction of the peritoneal thickness, and collagen‐I‐positive area in mice treated with CG. (A) haematoxylin and eosin staining, (B) Masson and (C) IHC staining of mice parietal peritoneum. (D‐E) S3I‐201 markedly decreased synthesis of Collagen I and a‐SMA in fibroblasts induced by CG, as assessed by western blot. * p <0.05; ** p <0.01; *** p <0.001.

Article Snippet: The STAT3‐specific inhibitor S3I‐201 (#SML0330) and CG (#C9394) were purchased from Sigma‐Aldrich (Darmstadt, Germany).

Techniques: Staining, Immunohistochemistry, Western Blot

Journal: Journal of Translational Medicine

Article Title: Short-chain fatty acid-producing bacterial strains attenuate experimental ulcerative colitis by promoting M2 macrophage polarization via JAK/STAT3/FOXO3 axis inactivation

doi: 10.1186/s12967-024-05122-w

Figure Lengend Snippet: Treatment with a mixture of seven short-chain fatty acid-producing bacterial strains (7-mix) and the resulting culture supernatant mixture (mix-sup) inhibits JAK/STAT3/FOXO3 pathway activation in macrophages. A-B . qRT-PCR analysis of the effect of 7-mix and mix-sup on the regulation of the JAK/STAT3/FOXO3 axis in colitic mice treated or not with clodronate (CLD) liposomes. C . Effect of 7-mix and mix-sup on the immunohistochemical expression of STAT3 and FOXO3 in colitic mice treated with CLD. D . Flow cytometry analysis of the effect of S3I-201 and fedratinib on macrophage polarization. E . qRT-PCR analysis of the effect of 7-mix, mix-sup, S3I-201, and fedratinib on the mRNA expression of JAK2, STAT3, and FOXO3 in LPS-treated RAW264.7 cells. F . Western blot analysis of the effect of 7-mix, mix-sup, S3I-201, and fedratinib on the protein expression of JAK2, p-JAK2, STAT3, p-STAT3, and FOXO3 in LPS-treated RAW264.7 cells. N = 3. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: To evaluate the effect of the STAT3 inhibitor S3I-201 (Abcam, UK) and the JAK2 inhibitor fedratinib (SAR302503, Selleck, China) on JAK/STAT3/FOXO3 signaling, cells were incubated with 7-mix (2 − 2 , namely 2.5 × 10 7 CFU), mix-sup (2 − 4 ), S3I-201 (50 µM), fedratinib (10 µM), or S3I-201 + fedratinib for 4 h.

Techniques: Activation Assay, Quantitative RT-PCR, Liposomes, Immunohistochemical staining, Expressing, Flow Cytometry, Western Blot

Journal: Journal of Translational Medicine

Article Title: Short-chain fatty acid-producing bacterial strains attenuate experimental ulcerative colitis by promoting M2 macrophage polarization via JAK/STAT3/FOXO3 axis inactivation

doi: 10.1186/s12967-024-05122-w

Figure Lengend Snippet: Schematic representation of the regulation of dextran sulfate sodium-induced colitis by short-chain fatty acid (SCFA)-producing bacterial strains and SCFAs. Colitis damages the gut barrier, inducing the activation of M1 macrophages, which produce pro-inflammatory cytokines, including IL-1β, IL-6, and TNF-α. In turn, SCFA-producing bacterial strains and SCFAs stimulate the activation of M2 macrophages, which produce anti-inflammatory cytokines (i.e., IL-10) to inhibit JAK/STAT3/FOXO3 axis activation and improve gut barrier function

Article Snippet: To evaluate the effect of the STAT3 inhibitor S3I-201 (Abcam, UK) and the JAK2 inhibitor fedratinib (SAR302503, Selleck, China) on JAK/STAT3/FOXO3 signaling, cells were incubated with 7-mix (2 − 2 , namely 2.5 × 10 7 CFU), mix-sup (2 − 4 ), S3I-201 (50 µM), fedratinib (10 µM), or S3I-201 + fedratinib for 4 h.

Techniques: Activation Assay

Journal: International Journal of Molecular Sciences

Article Title: Regulation of Cathelicidin Antimicrobial Peptide (CAMP) Gene Expression by TNFα and cfDNA in Adipocytes

doi: 10.3390/ijms242115820

Figure Lengend Snippet: TNFα-induced CAMP gene expression is antagonized by inhibitor of PI3K signaling: TNFα induces CAMP expression in 3T3-L1 adipocytes ( A ). Inhibition of the PI3K pathway by 5 µM LY294002 antagonizes TNFα-induced CAMP expression, whereas inhibition of the NF-κB (5 µM BAY11-7085) ( B ), STAT3 (50 µM S3I-201), MAPK (5 µM SB239063), and MEK-1/-2 (5 µM U0126) pathways does not inhibit TNFα-induced CAMP expression ( C ). Basal CAMP expression is not significantly modified by inhibition of classical signal transduction pathways ( D ) (* p < 0.05, ** p < 0.01). N = 9–18 samples were investigated. Gene expression levels are given in relative gene expression as compared to control.

Article Snippet: Furthermore, co-stimulation experiments were performed with 10 ng/mL TNFα and inhibitors of different signal transduction pathways (NF-κB inhibitor BAY-11 (5 mM), STAT3 inhibitor S3I-201 (50 mM), selective MAPK inhibitor SB239063 (5 mM), MEK-1/-2 inhibitor U0126 (5 mM), and phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 (5 mM), all purchased from Merck).

Techniques: Expressing, Inhibition, Modification, Transduction