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TaKaRa infected e coli xl1 blue bacterial cells
Infected E Coli Xl1 Blue Bacterial Cells, supplied by TaKaRa, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/infected e coli xl1 blue bacterial cells/product/TaKaRa
Average 87 stars, based on 1 article reviews
Price from $9.99 to $1999.99
infected e coli xl1 blue bacterial cells - by Bioz Stars, 2020-04
87/100 stars

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Clone Assay:

Article Title: Spit and venom from Scytodes spiders: a diverse and distinct cocktail
Article Snippet: Upon ligation into the λTriplEx2 vector, the cDNAs were packaged into MaxPlax™ lambda packaging extracts (Epicentre) according the manufacturer’s protocol and infected E. coli XL1-Blue bacterial cells (Clontech). .. These mixtures were incubated for 2 hrs at room temperature and stored at 4° C. From the LC library 1,588 clones were PCR screened to identify cDNA inserts ≥500 bp.

Agarose Gel Electrophoresis:

Article Title: Spit and venom from Scytodes spiders: a diverse and distinct cocktail
Article Snippet: Upon ligation into the λTriplEx2 vector, the cDNAs were packaged into MaxPlax™ lambda packaging extracts (Epicentre) according the manufacturer’s protocol and infected E. coli XL1-Blue bacterial cells (Clontech). .. Each reaction contained 1 μ l of eluted cDNA, the λTriplEx2 primers that flank the insert (Clontech, 0.2 μ M final concentration), 2X MasterAmp™ F PCR Premix (Epicentre, 1x final concentration) and Taq polymerase (New England Biolabs, 0.05 U/ μ l final concentration) with cycling parameters of 95° C for 1 min 30 sec followed by 30 cycles of 95° C for 1 min/66° C for 1 min/72° C for 1 min, and a final extension at 72° C for 7 min. PCR products were checked for size on a 1% agarose gel compared to a 1kb DNA mass ladder (Invitrogen) and stained with SYBR® Safe (Invitrogen).

Ligation:

Article Title: Solution Structures of Two Homologous Venom Peptides from Sicarius dolichocephalus
Article Snippet: .. Upon ligation into the λTriplEx2 vector, we packaged the cDNAs into MaxPlax™ lambda packaging extracts (Epicentre) according to the manufacturer's protocol and infected Escherichia coli XL1-Blue bacterial cells (Clontech). ..

Article Title: Spit and venom from Scytodes spiders: a diverse and distinct cocktail
Article Snippet: .. Upon ligation into the λTriplEx2 vector, the cDNAs were packaged into MaxPlax™ lambda packaging extracts (Epicentre) according the manufacturer’s protocol and infected E. coli XL1-Blue bacterial cells (Clontech). ..

cDNA Library Assay:

Article Title: Solution Structures of Two Homologous Venom Peptides from Sicarius dolichocephalus
Article Snippet: Using this RNA, we constructed a cDNA library using the SMART™ cDNA library construction kit (Clontech), which is optimized for making complete, full-length cDNAs from small amounts of starting material. .. Upon ligation into the λTriplEx2 vector, we packaged the cDNAs into MaxPlax™ lambda packaging extracts (Epicentre) according to the manufacturer's protocol and infected Escherichia coli XL1-Blue bacterial cells (Clontech).

Article Title: Spit and venom from Scytodes spiders: a diverse and distinct cocktail
Article Snippet: Paragraph title: Venom gland and RNA isolation, cDNA library construction and screening ... Upon ligation into the λTriplEx2 vector, the cDNAs were packaged into MaxPlax™ lambda packaging extracts (Epicentre) according the manufacturer’s protocol and infected E. coli XL1-Blue bacterial cells (Clontech).

Isolation:

Article Title: Solution Structures of Two Homologous Venom Peptides from Sicarius dolichocephalus
Article Snippet: We isolated total RNA from a single S. dolichocephalus spider using the ChargeSwitch® Total RNA Cell Kit (Invitrogen). .. Upon ligation into the λTriplEx2 vector, we packaged the cDNAs into MaxPlax™ lambda packaging extracts (Epicentre) according to the manufacturer's protocol and infected Escherichia coli XL1-Blue bacterial cells (Clontech).

Article Title: Spit and venom from Scytodes spiders: a diverse and distinct cocktail
Article Snippet: Paragraph title: Venom gland and RNA isolation, cDNA library construction and screening ... Upon ligation into the λTriplEx2 vector, the cDNAs were packaged into MaxPlax™ lambda packaging extracts (Epicentre) according the manufacturer’s protocol and infected E. coli XL1-Blue bacterial cells (Clontech).

Size-exclusion Chromatography:

Article Title: Spit and venom from Scytodes spiders: a diverse and distinct cocktail
Article Snippet: Upon ligation into the λTriplEx2 vector, the cDNAs were packaged into MaxPlax™ lambda packaging extracts (Epicentre) according the manufacturer’s protocol and infected E. coli XL1-Blue bacterial cells (Clontech). .. Each reaction contained 1 μ l of eluted cDNA, the λTriplEx2 primers that flank the insert (Clontech, 0.2 μ M final concentration), 2X MasterAmp™ F PCR Premix (Epicentre, 1x final concentration) and Taq polymerase (New England Biolabs, 0.05 U/ μ l final concentration) with cycling parameters of 95° C for 1 min 30 sec followed by 30 cycles of 95° C for 1 min/66° C for 1 min/72° C for 1 min, and a final extension at 72° C for 7 min. PCR products were checked for size on a 1% agarose gel compared to a 1kb DNA mass ladder (Invitrogen) and stained with SYBR® Safe (Invitrogen).

Construct:

Article Title: Solution Structures of Two Homologous Venom Peptides from Sicarius dolichocephalus
Article Snippet: Using this RNA, we constructed a cDNA library using the SMART™ cDNA library construction kit (Clontech), which is optimized for making complete, full-length cDNAs from small amounts of starting material. .. Upon ligation into the λTriplEx2 vector, we packaged the cDNAs into MaxPlax™ lambda packaging extracts (Epicentre) according to the manufacturer's protocol and infected Escherichia coli XL1-Blue bacterial cells (Clontech).

Article Title: Spit and venom from Scytodes spiders: a diverse and distinct cocktail
Article Snippet: The LC venom gland cDNA library was constructed using the SMART™ cDNA library construction kit (Clontech), which is optimized for making complete, full-length cDNAs. .. Upon ligation into the λTriplEx2 vector, the cDNAs were packaged into MaxPlax™ lambda packaging extracts (Epicentre) according the manufacturer’s protocol and infected E. coli XL1-Blue bacterial cells (Clontech).

Purification:

Article Title: Solution Structures of Two Homologous Venom Peptides from Sicarius dolichocephalus
Article Snippet: We used 72 ng of total RNA from S. dolichocephalus for first strand synthesis of cDNA and followed the manufacturer's protocol for library construction, with the exception of using CHROMA SPIN™ columns (Clontech) for size fractionation and purification of cDNAs. .. Upon ligation into the λTriplEx2 vector, we packaged the cDNAs into MaxPlax™ lambda packaging extracts (Epicentre) according to the manufacturer's protocol and infected Escherichia coli XL1-Blue bacterial cells (Clontech).

Concentration Assay:

Article Title: Solution Structures of Two Homologous Venom Peptides from Sicarius dolichocephalus
Article Snippet: Upon ligation into the λTriplEx2 vector, we packaged the cDNAs into MaxPlax™ lambda packaging extracts (Epicentre) according to the manufacturer's protocol and infected Escherichia coli XL1-Blue bacterial cells (Clontech). .. We identified cDNA inserts larger than 500 bp with a PCR screen that used λTriplEx2 primers that flank the insert (Clontech, 0.2 µM final concentration), 2X MasterAmp™ F PCR Premix (Epicentre, 1× final concentration) and Taq polymerase (New England Biolabs, 0.05 U/µL final concentration).

Article Title: Spit and venom from Scytodes spiders: a diverse and distinct cocktail
Article Snippet: Upon ligation into the λTriplEx2 vector, the cDNAs were packaged into MaxPlax™ lambda packaging extracts (Epicentre) according the manufacturer’s protocol and infected E. coli XL1-Blue bacterial cells (Clontech). .. Each reaction contained 1 μ l of eluted cDNA, the λTriplEx2 primers that flank the insert (Clontech, 0.2 μ M final concentration), 2X MasterAmp™ F PCR Premix (Epicentre, 1x final concentration) and Taq polymerase (New England Biolabs, 0.05 U/ μ l final concentration) with cycling parameters of 95° C for 1 min 30 sec followed by 30 cycles of 95° C for 1 min/66° C for 1 min/72° C for 1 min, and a final extension at 72° C for 7 min. PCR products were checked for size on a 1% agarose gel compared to a 1kb DNA mass ladder (Invitrogen) and stained with SYBR® Safe (Invitrogen).

Incubation:

Article Title: Spit and venom from Scytodes spiders: a diverse and distinct cocktail
Article Snippet: Upon ligation into the λTriplEx2 vector, the cDNAs were packaged into MaxPlax™ lambda packaging extracts (Epicentre) according the manufacturer’s protocol and infected E. coli XL1-Blue bacterial cells (Clontech). .. These mixtures were incubated for 2 hrs at room temperature and stored at 4° C. From the LC library 1,588 clones were PCR screened to identify cDNA inserts ≥500 bp.

Polymerase Chain Reaction:

Article Title: Solution Structures of Two Homologous Venom Peptides from Sicarius dolichocephalus
Article Snippet: Upon ligation into the λTriplEx2 vector, we packaged the cDNAs into MaxPlax™ lambda packaging extracts (Epicentre) according to the manufacturer's protocol and infected Escherichia coli XL1-Blue bacterial cells (Clontech). .. We identified cDNA inserts larger than 500 bp with a PCR screen that used λTriplEx2 primers that flank the insert (Clontech, 0.2 µM final concentration), 2X MasterAmp™ F PCR Premix (Epicentre, 1× final concentration) and Taq polymerase (New England Biolabs, 0.05 U/µL final concentration).

Article Title: Spit and venom from Scytodes spiders: a diverse and distinct cocktail
Article Snippet: Upon ligation into the λTriplEx2 vector, the cDNAs were packaged into MaxPlax™ lambda packaging extracts (Epicentre) according the manufacturer’s protocol and infected E. coli XL1-Blue bacterial cells (Clontech). .. These mixtures were incubated for 2 hrs at room temperature and stored at 4° C. From the LC library 1,588 clones were PCR screened to identify cDNA inserts ≥500 bp.

Fractionation:

Article Title: Solution Structures of Two Homologous Venom Peptides from Sicarius dolichocephalus
Article Snippet: We used 72 ng of total RNA from S. dolichocephalus for first strand synthesis of cDNA and followed the manufacturer's protocol for library construction, with the exception of using CHROMA SPIN™ columns (Clontech) for size fractionation and purification of cDNAs. .. Upon ligation into the λTriplEx2 vector, we packaged the cDNAs into MaxPlax™ lambda packaging extracts (Epicentre) according to the manufacturer's protocol and infected Escherichia coli XL1-Blue bacterial cells (Clontech).

Infection:

Article Title: Solution Structures of Two Homologous Venom Peptides from Sicarius dolichocephalus
Article Snippet: .. Upon ligation into the λTriplEx2 vector, we packaged the cDNAs into MaxPlax™ lambda packaging extracts (Epicentre) according to the manufacturer's protocol and infected Escherichia coli XL1-Blue bacterial cells (Clontech). ..

Article Title: Spit and venom from Scytodes spiders: a diverse and distinct cocktail
Article Snippet: .. Upon ligation into the λTriplEx2 vector, the cDNAs were packaged into MaxPlax™ lambda packaging extracts (Epicentre) according the manufacturer’s protocol and infected E. coli XL1-Blue bacterial cells (Clontech). ..

Sequencing:

Article Title: Solution Structures of Two Homologous Venom Peptides from Sicarius dolichocephalus
Article Snippet: Upon ligation into the λTriplEx2 vector, we packaged the cDNAs into MaxPlax™ lambda packaging extracts (Epicentre) according to the manufacturer's protocol and infected Escherichia coli XL1-Blue bacterial cells (Clontech). .. We performed PCR with an initial denaturing step of 95°C for 1.5 min followed by 30 cycles of 95°C for 1 min/66°C for 1 min/72°C for 1 min, and then a final extension at 72°C for 7 min. For sequencing we converted the λ vector-containing products to plasmid products (pTriplEx2) using E. coli BM25.8 cells provided with the library kit (Clontech).

Staining:

Article Title: Spit and venom from Scytodes spiders: a diverse and distinct cocktail
Article Snippet: Upon ligation into the λTriplEx2 vector, the cDNAs were packaged into MaxPlax™ lambda packaging extracts (Epicentre) according the manufacturer’s protocol and infected E. coli XL1-Blue bacterial cells (Clontech). .. Each reaction contained 1 μ l of eluted cDNA, the λTriplEx2 primers that flank the insert (Clontech, 0.2 μ M final concentration), 2X MasterAmp™ F PCR Premix (Epicentre, 1x final concentration) and Taq polymerase (New England Biolabs, 0.05 U/ μ l final concentration) with cycling parameters of 95° C for 1 min 30 sec followed by 30 cycles of 95° C for 1 min/66° C for 1 min/72° C for 1 min, and a final extension at 72° C for 7 min. PCR products were checked for size on a 1% agarose gel compared to a 1kb DNA mass ladder (Invitrogen) and stained with SYBR® Safe (Invitrogen).

Plasmid Preparation:

Article Title: Solution Structures of Two Homologous Venom Peptides from Sicarius dolichocephalus
Article Snippet: .. Upon ligation into the λTriplEx2 vector, we packaged the cDNAs into MaxPlax™ lambda packaging extracts (Epicentre) according to the manufacturer's protocol and infected Escherichia coli XL1-Blue bacterial cells (Clontech). ..

Article Title: Spit and venom from Scytodes spiders: a diverse and distinct cocktail
Article Snippet: .. Upon ligation into the λTriplEx2 vector, the cDNAs were packaged into MaxPlax™ lambda packaging extracts (Epicentre) according the manufacturer’s protocol and infected E. coli XL1-Blue bacterial cells (Clontech). ..

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    TaKaRa infected escherichia coli xl1 blue bacterial cells
    Infected Escherichia Coli Xl1 Blue Bacterial Cells, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/infected escherichia coli xl1 blue bacterial cells/product/TaKaRa
    Average 88 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    infected escherichia coli xl1 blue bacterial cells - by Bioz Stars, 2020-04
    88/100 stars
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