in vitro transcription translation analysis in vitro translation transcription reactions  (New England Biolabs)


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    New England Biolabs in vitro transcription translation analysis in vitro translation transcription reactions
    <t>In</t> <t>vitro</t> <t>transcription-translation</t> <t>analysis</t> of H 2 S n activation of OxyR and its mutants.
    In Vitro Transcription Translation Analysis In Vitro Translation Transcription Reactions, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/in vitro transcription translation analysis in vitro translation transcription reactions/product/New England Biolabs
    Average 86 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    in vitro transcription translation analysis in vitro translation transcription reactions - by Bioz Stars, 2022-10
    86/100 stars

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    1) Product Images from "OxyR senses reactive sulfane sulfur and activates genes for its removal inEscherichia coli"

    Article Title: OxyR senses reactive sulfane sulfur and activates genes for its removal inEscherichia coli

    Journal: bioRxiv

    doi: 10.1101/561019

    In vitro transcription-translation analysis of H 2 S n activation of OxyR and its mutants.
    Figure Legend Snippet: In vitro transcription-translation analysis of H 2 S n activation of OxyR and its mutants.

    Techniques Used: In Vitro, Activation Assay

    2) Product Images from "OxyR senses sulfane sulfur and activates the genes for its removal in Escherichia coli"

    Article Title: OxyR senses sulfane sulfur and activates the genes for its removal in Escherichia coli

    Journal: Redox Biology

    doi: 10.1016/j.redox.2019.101293

    In vitro transcription-translation analysis of H 2 S n activation of OxyR and its mutants. Purified OxyR and its mutants were treated with DTT to ensure their thiols were in the reduce form; The proteins were then treated with H 2 S n to generate H 2 S n modified protein. The in vitro transcription-translation system contained P trxC -mKate DNA fragment (200 ng) and DTT-reduced or H 2 S n -treated OxyR (500 ng), and the expressed mKate was analyzed with the fluorescence photometer Synergy H1. (n ≥ 3 for each group) Data information: Data are presented as mean ± SEM.
    Figure Legend Snippet: In vitro transcription-translation analysis of H 2 S n activation of OxyR and its mutants. Purified OxyR and its mutants were treated with DTT to ensure their thiols were in the reduce form; The proteins were then treated with H 2 S n to generate H 2 S n modified protein. The in vitro transcription-translation system contained P trxC -mKate DNA fragment (200 ng) and DTT-reduced or H 2 S n -treated OxyR (500 ng), and the expressed mKate was analyzed with the fluorescence photometer Synergy H1. (n ≥ 3 for each group) Data information: Data are presented as mean ± SEM.

    Techniques Used: In Vitro, Activation Assay, Purification, Modification, Fluorescence

    Comparison of the activation effect of H 2 S n and H 2 O 2 . A-C H 2 S n or H 2 O 2 (100–600 μM) was used to treat E. coli wt strains containing reporter plasmids. (n ≥ 3 for each group) D Purified OxyR and its mutants were treated with DTT to ensure their thiols were in the reduce form; The proteins were then treated with H 2 S n or H 2 O 2 to generate H 2 S n - or H 2 O 2 -modified OxyR. The in vitro transcription-translation system contained P trxC -mKate DNA fragment (200 ng) and DTT-reduced, H 2 S n - or H 2 O 2 -treated OxyR (500 ng) and the expressed mKate was analyzed with the fluorescence photometer Synergy H1. (n ≥ 3 for each group) E H 2 S n or H 2 O 2 (400 μM) was used to treat E. coli wt. RT-qPCR was used to quantify the expression of trxC . (n ≥ 3 for each group).
    Figure Legend Snippet: Comparison of the activation effect of H 2 S n and H 2 O 2 . A-C H 2 S n or H 2 O 2 (100–600 μM) was used to treat E. coli wt strains containing reporter plasmids. (n ≥ 3 for each group) D Purified OxyR and its mutants were treated with DTT to ensure their thiols were in the reduce form; The proteins were then treated with H 2 S n or H 2 O 2 to generate H 2 S n - or H 2 O 2 -modified OxyR. The in vitro transcription-translation system contained P trxC -mKate DNA fragment (200 ng) and DTT-reduced, H 2 S n - or H 2 O 2 -treated OxyR (500 ng) and the expressed mKate was analyzed with the fluorescence photometer Synergy H1. (n ≥ 3 for each group) E H 2 S n or H 2 O 2 (400 μM) was used to treat E. coli wt. RT-qPCR was used to quantify the expression of trxC . (n ≥ 3 for each group).

    Techniques Used: Activation Assay, Purification, Modification, In Vitro, Fluorescence, Quantitative RT-PCR, Expressing

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    New England Biolabs in vitro transcription translation analysis in vitro translation transcription reactions
    <t>In</t> <t>vitro</t> <t>transcription-translation</t> <t>analysis</t> of H 2 S n activation of OxyR and its mutants. Purified OxyR and its mutants were treated with DTT to ensure their thiols were in the reduce form; The proteins were then treated with H 2 S n to generate H 2 S n modified protein. The in vitro transcription-translation system contained P trxC -mKate DNA fragment (200 ng) and DTT-reduced or H 2 S n -treated OxyR (500 ng), and the expressed mKate was analyzed with the fluorescence photometer Synergy H1. (n ≥ 3 for each group) Data information: Data are presented as mean ± SEM.
    In Vitro Transcription Translation Analysis In Vitro Translation Transcription Reactions, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/in vitro transcription translation analysis in vitro translation transcription reactions/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    in vitro transcription translation analysis in vitro translation transcription reactions - by Bioz Stars, 2022-10
    86/100 stars
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    In vitro transcription-translation analysis of H 2 S n activation of OxyR and its mutants. Purified OxyR and its mutants were treated with DTT to ensure their thiols were in the reduce form; The proteins were then treated with H 2 S n to generate H 2 S n modified protein. The in vitro transcription-translation system contained P trxC -mKate DNA fragment (200 ng) and DTT-reduced or H 2 S n -treated OxyR (500 ng), and the expressed mKate was analyzed with the fluorescence photometer Synergy H1. (n ≥ 3 for each group) Data information: Data are presented as mean ± SEM.

    Journal: Redox Biology

    Article Title: OxyR senses sulfane sulfur and activates the genes for its removal in Escherichia coli

    doi: 10.1016/j.redox.2019.101293

    Figure Lengend Snippet: In vitro transcription-translation analysis of H 2 S n activation of OxyR and its mutants. Purified OxyR and its mutants were treated with DTT to ensure their thiols were in the reduce form; The proteins were then treated with H 2 S n to generate H 2 S n modified protein. The in vitro transcription-translation system contained P trxC -mKate DNA fragment (200 ng) and DTT-reduced or H 2 S n -treated OxyR (500 ng), and the expressed mKate was analyzed with the fluorescence photometer Synergy H1. (n ≥ 3 for each group) Data information: Data are presented as mean ± SEM.

    Article Snippet: 2.8 In vitro transcription-translation analysis In vitro translation-transcription reactions were performed using the Purexpress In Vitro Protein Synthesis system (NEB #E6800).

    Techniques: In Vitro, Activation Assay, Purification, Modification, Fluorescence

    Comparison of the activation effect of H 2 S n and H 2 O 2 . A-C H 2 S n or H 2 O 2 (100–600 μM) was used to treat E. coli wt strains containing reporter plasmids. (n ≥ 3 for each group) D Purified OxyR and its mutants were treated with DTT to ensure their thiols were in the reduce form; The proteins were then treated with H 2 S n or H 2 O 2 to generate H 2 S n - or H 2 O 2 -modified OxyR. The in vitro transcription-translation system contained P trxC -mKate DNA fragment (200 ng) and DTT-reduced, H 2 S n - or H 2 O 2 -treated OxyR (500 ng) and the expressed mKate was analyzed with the fluorescence photometer Synergy H1. (n ≥ 3 for each group) E H 2 S n or H 2 O 2 (400 μM) was used to treat E. coli wt. RT-qPCR was used to quantify the expression of trxC . (n ≥ 3 for each group).

    Journal: Redox Biology

    Article Title: OxyR senses sulfane sulfur and activates the genes for its removal in Escherichia coli

    doi: 10.1016/j.redox.2019.101293

    Figure Lengend Snippet: Comparison of the activation effect of H 2 S n and H 2 O 2 . A-C H 2 S n or H 2 O 2 (100–600 μM) was used to treat E. coli wt strains containing reporter plasmids. (n ≥ 3 for each group) D Purified OxyR and its mutants were treated with DTT to ensure their thiols were in the reduce form; The proteins were then treated with H 2 S n or H 2 O 2 to generate H 2 S n - or H 2 O 2 -modified OxyR. The in vitro transcription-translation system contained P trxC -mKate DNA fragment (200 ng) and DTT-reduced, H 2 S n - or H 2 O 2 -treated OxyR (500 ng) and the expressed mKate was analyzed with the fluorescence photometer Synergy H1. (n ≥ 3 for each group) E H 2 S n or H 2 O 2 (400 μM) was used to treat E. coli wt. RT-qPCR was used to quantify the expression of trxC . (n ≥ 3 for each group).

    Article Snippet: 2.8 In vitro transcription-translation analysis In vitro translation-transcription reactions were performed using the Purexpress In Vitro Protein Synthesis system (NEB #E6800).

    Techniques: Activation Assay, Purification, Modification, In Vitro, Fluorescence, Quantitative RT-PCR, Expressing