in vitro antigen presenting cell assay twenty four h  (Worthington Biochemical)


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    Worthington Biochemical in vitro antigen presenting cell assay twenty four h
    Vitamin D deficiency does not affect the immunogenicity of dendritic cells or the numbers of regulatory T cells in the airway-draining lymph nodes. Eight week-old male and female offspring born to vitamin D-replete or -deficient mothers and maintained on the vitamin D-containing or -null diets (respectively) were sensitized intraperitoneally with 1 µg ovalbumin <t>(OVA)</t> (0.2 mg Aluminium hydroxide (Alum)). Mice were boosted with the same OVA/Alum dose two weeks after the initial sensitization, and one week later their airways challenged with aerosolised OVA (1% in saline). Cells were assessed twenty-four h after OVA challenge. In (A), the capacity of CD11c+ cells isolated from the ADLN to process and present antigen to co-cultured CD4+ T cells from DO11.10 mice was determined by [ 3 H]-thymidine incorporation for the final 24 h of 96 h co-culture with 1 µg/ml OVA peptide. Results are shown of a representative of 3 independent experiments (mean±SEM) for CD11c+ cells cultured at various ratios with CD4+ cells. In (B), the FACS gating strategy used to determine the percentage of ClassII+CD11c+CD8− cells in ADLN for 8–9 mice per treatment pooled from 3 independent experiments (mean±SEM). In (C), the FACS gating strategy used to determine the percentage of CD3+CD4+ T cells and CD3+CD4+CD25+Foxp3+ and CD3+CD4+CD25+Foxp3− cells in the ADLN for 8–9 mice per treatment pooled from 3 independent experiments (mean±SEM). In (D), the expression of CCL5, CCL11, CCL19, CCL20, CXCL1 and IL-18RA mRNAs in CD11c+ cells isolated from pooled ADLN of ten mice per treatment with results shown from 3 independent experiments (mean+SEM). (*p
    In Vitro Antigen Presenting Cell Assay Twenty Four H, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    in vitro antigen presenting cell assay twenty four h - by Bioz Stars, 2021-03
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    1) Product Images from "Reversible Control by Vitamin D of Granulocytes and Bacteria in the Lungs of Mice: An Ovalbumin-Induced Model of Allergic Airway Disease"

    Article Title: Reversible Control by Vitamin D of Granulocytes and Bacteria in the Lungs of Mice: An Ovalbumin-Induced Model of Allergic Airway Disease

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0067823

    Vitamin D deficiency does not affect the immunogenicity of dendritic cells or the numbers of regulatory T cells in the airway-draining lymph nodes. Eight week-old male and female offspring born to vitamin D-replete or -deficient mothers and maintained on the vitamin D-containing or -null diets (respectively) were sensitized intraperitoneally with 1 µg ovalbumin (OVA) (0.2 mg Aluminium hydroxide (Alum)). Mice were boosted with the same OVA/Alum dose two weeks after the initial sensitization, and one week later their airways challenged with aerosolised OVA (1% in saline). Cells were assessed twenty-four h after OVA challenge. In (A), the capacity of CD11c+ cells isolated from the ADLN to process and present antigen to co-cultured CD4+ T cells from DO11.10 mice was determined by [ 3 H]-thymidine incorporation for the final 24 h of 96 h co-culture with 1 µg/ml OVA peptide. Results are shown of a representative of 3 independent experiments (mean±SEM) for CD11c+ cells cultured at various ratios with CD4+ cells. In (B), the FACS gating strategy used to determine the percentage of ClassII+CD11c+CD8− cells in ADLN for 8–9 mice per treatment pooled from 3 independent experiments (mean±SEM). In (C), the FACS gating strategy used to determine the percentage of CD3+CD4+ T cells and CD3+CD4+CD25+Foxp3+ and CD3+CD4+CD25+Foxp3− cells in the ADLN for 8–9 mice per treatment pooled from 3 independent experiments (mean±SEM). In (D), the expression of CCL5, CCL11, CCL19, CCL20, CXCL1 and IL-18RA mRNAs in CD11c+ cells isolated from pooled ADLN of ten mice per treatment with results shown from 3 independent experiments (mean+SEM). (*p
    Figure Legend Snippet: Vitamin D deficiency does not affect the immunogenicity of dendritic cells or the numbers of regulatory T cells in the airway-draining lymph nodes. Eight week-old male and female offspring born to vitamin D-replete or -deficient mothers and maintained on the vitamin D-containing or -null diets (respectively) were sensitized intraperitoneally with 1 µg ovalbumin (OVA) (0.2 mg Aluminium hydroxide (Alum)). Mice were boosted with the same OVA/Alum dose two weeks after the initial sensitization, and one week later their airways challenged with aerosolised OVA (1% in saline). Cells were assessed twenty-four h after OVA challenge. In (A), the capacity of CD11c+ cells isolated from the ADLN to process and present antigen to co-cultured CD4+ T cells from DO11.10 mice was determined by [ 3 H]-thymidine incorporation for the final 24 h of 96 h co-culture with 1 µg/ml OVA peptide. Results are shown of a representative of 3 independent experiments (mean±SEM) for CD11c+ cells cultured at various ratios with CD4+ cells. In (B), the FACS gating strategy used to determine the percentage of ClassII+CD11c+CD8− cells in ADLN for 8–9 mice per treatment pooled from 3 independent experiments (mean±SEM). In (C), the FACS gating strategy used to determine the percentage of CD3+CD4+ T cells and CD3+CD4+CD25+Foxp3+ and CD3+CD4+CD25+Foxp3− cells in the ADLN for 8–9 mice per treatment pooled from 3 independent experiments (mean±SEM). In (D), the expression of CCL5, CCL11, CCL19, CCL20, CXCL1 and IL-18RA mRNAs in CD11c+ cells isolated from pooled ADLN of ten mice per treatment with results shown from 3 independent experiments (mean+SEM). (*p

    Techniques Used: Mouse Assay, Isolation, Cell Culture, Co-Culture Assay, FACS, Expressing

    Vitamin D supplementation suppresses circulating neutrophil and basophil levels in male mice. Male and female offspring born to vitamin D-replete or -deficient mothers and maintained on the vitamin D-replete or null diets (respectively), or vitamin D-deficient offspring switched to a vitamin D-replete diet from 8 weeks of age (VitD− to vitD+, −/+), were sensitised at 12 weeks of age intraperitoneally with 1 µg ovalbumin (OVA) (0.2 mg Aluminium hydroxide (Alum)). Mice were boosted with the same OVA/Alum dose two weeks after the initial sensitisation, and one week later their airways challenged with aerosolised OVA (1% in saline). Twenty-four h after the OVA challenge, the proportions of eosinophils, neutrophils and basophils of circulating white blood cells (WBC) is shown as mean + SEM for n = 5 mice/treatment for a representative experiment.
    Figure Legend Snippet: Vitamin D supplementation suppresses circulating neutrophil and basophil levels in male mice. Male and female offspring born to vitamin D-replete or -deficient mothers and maintained on the vitamin D-replete or null diets (respectively), or vitamin D-deficient offspring switched to a vitamin D-replete diet from 8 weeks of age (VitD− to vitD+, −/+), were sensitised at 12 weeks of age intraperitoneally with 1 µg ovalbumin (OVA) (0.2 mg Aluminium hydroxide (Alum)). Mice were boosted with the same OVA/Alum dose two weeks after the initial sensitisation, and one week later their airways challenged with aerosolised OVA (1% in saline). Twenty-four h after the OVA challenge, the proportions of eosinophils, neutrophils and basophils of circulating white blood cells (WBC) is shown as mean + SEM for n = 5 mice/treatment for a representative experiment.

    Techniques Used: Mouse Assay

    Related Articles

    In Vitro:

    Article Title: Reversible Control by Vitamin D of Granulocytes and Bacteria in the Lungs of Mice: An Ovalbumin-Induced Model of Allergic Airway Disease
    Article Snippet: At day 20 (24 h before OVA challenge) of the OVA-induced allergic airway disease model, sera were tested for the presence of GM-CSF by ELISA as previously described , , using antibody pairs from eBiosciences. .. In vitro antigen-presenting cell assay Twenty-four h after OVA challenge, ADLN were removed and digested with collagenase type 4 (1 mg/ml, Worthington Biochemical, Lakewood, NJ) and DNAse (0.1 mg/ml, Sigma) for 30 min at 37°C. .. CD11c microbeads (Miltenyi Biotech) were used with the autoMACS separator (Miltenyi Biotech) to positively select for CD11c+ cells.

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