in fusion hd ecodry cloning plus systems kit  (TaKaRa)

 
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    Name:
    In Fusion HD EcoDry Cloning Plus
    Description:
    In Fusion HD EcoDry Cloning Plus products provide our In Fusion HD cloning enzyme mix in a lyophilized ready to use format that can be stored at room temperature The cloning reagent is prealiquoted and dried down in tubes that are covered with an aluminum seal This convenient cloning enzyme format is stable at room temperature making it ideal for high throughput cloning with robotic liquid handlers
    Catalog Number:
    638915
    Price:
    None
    Size:
    96 Rxns
    Category:
    In Fusion HD EcoDry Cloning Plus In Fusion Cloning Cloning
    Buy from Supplier


    Structured Review

    TaKaRa in fusion hd ecodry cloning plus systems kit
    In Fusion HD EcoDry Cloning Plus products provide our In Fusion HD cloning enzyme mix in a lyophilized ready to use format that can be stored at room temperature The cloning reagent is prealiquoted and dried down in tubes that are covered with an aluminum seal This convenient cloning enzyme format is stable at room temperature making it ideal for high throughput cloning with robotic liquid handlers
    https://www.bioz.com/result/in fusion hd ecodry cloning plus systems kit/product/TaKaRa
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    in fusion hd ecodry cloning plus systems kit - by Bioz Stars, 2020-07
    94/100 stars

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    Related Articles

    Clone Assay:

    Article Title: C11orf95-RELA fusions drive oncogenic NF-κB signaling in ependymoma
    Article Snippet: .. The Clontech In-Fusion HD EcoDry Cloning Plus system was used to generate fusion constructs. .. All constructs were verified by sequencing and used to make retroviruses as described .

    Article Title: Discovery and Characterization of a Novel CD4-Binding Adnectin with Potent Anti-HIV Activity
    Article Snippet: .. The envelope genes were cloned into the pCMV-HA expression vector (Clontech) using the In-Fusion HD EcoDry technology (Clontech) and were used in the assay to evaluate a wide spectrum of subtypes. ..

    Article Title: TRIOBP-5 sculpts stereocilia rootlets and stiffens supporting cells enabling hearing
    Article Snippet: .. The Triobp- 5Δcc expression vector in which the coiled-coil domains were deleted was constructed using 3 fragments from the full-length Triobp-5 cDNA that were combined using In-Fusion cloning (Takara, 638912), such that sequences encoded by exons 11, 12, and 15–22 were deleted. .. For transfection into HeLa cells in 6-well dishes, we used expression vectors (equal molar amounts for a total of 2.0 μg of DNA) that separately express AcGFP1-TRIOBP-5, DsRed-TRIOBP-5, and the MYO10NANOTRAP per well.

    Article Title: Fibroblast activation protein (FAP) as a novel metabolic target
    Article Snippet: .. Amplified LIC-Sumo vector and FGF21 gene fragment were mixed together, treated using In-Fusion HD EcoDry Cloning Plus systems kit and transfected into stellar E. coli competent cells (both Clontech). .. Positive clones were selected on ampicillin/LB agar plate with single colonies picked to amplify plasmids with QIA prep miniprep kit (Qiagen).

    Article Title: Activity of the HIV-1 Attachment Inhibitor BMS-626529, the Active Component of the Prodrug BMS-663068, against CD4-Independent Viruses and HIV-1 Envelopes Resistant to Other Entry Inhibitors
    Article Snippet: .. Briefly, env clones were amplified by reverse transcriptase PCR (RT-PCR) and verified by sequencing, and the PCR products were cloned into the pCMV-HA expression vector (Clontech) by using In-Fusion HD EcoDry technology (Clontech) and transfected into cells to create effector cells. .. Cells were transfected with equal amounts of envelope-expressing plasmid DNA (50 ng/well in 6-well plates).

    Article Title: Promotion of Axon Growth by the Secreted End of a Transcription Factor
    Article Snippet: .. First, the open reading frame (ORF) of CREB3L2 was subcloned into a vector containing GFP (Takara, pEGFP-C1) using the In Fusion HD Cloning Plus kit (Takara, 638913). .. Afterward, the ORF for mCherry (Takara, pmCherry-C1, 632524) was amplified and inserted into the GFP-CREB3L2 construct using the In Fusion kit.

    Article Title: PRMT1-mediated methylation of the microprocessor-associated proteins regulates microRNA biogenesis
    Article Snippet: .. To overexpress PRMT1, the cDNA of the v2 isoform was amplified starting from HeLa cDNA, using the following primers: FW 5′-GGGGATCCCCCGGGCTGCAGATGGCGGCAGCCGAGGCCGCGAACTGCA-3′ REV 5′-GAGGTTGATTGTCGACTCAGCGCATCCGGTAGTCGGTGG-3′ The cDNA of PRMT1 was cloned into the pCCLsin.CPPT.PGK.GFP.WPRE lentiviral vector after plasmid linearization with PstI and SalI using the in-fusion HD EcoDry cloning plus, according to the manufacturer's instructions (Clontech Laboratories, Inc., CA, USA). .. The GFP cassette was removed from the vector upon digestion with XhoI.

    Article Title: Multi-color imaging of the bacterial nucleoid and division proteins with blue, orange, and near-infrared fluorescent proteins
    Article Snippet: .. Plasmid and Strain Construction The plasmids were constructed using Gateway cloning kit (Invitrogen, catalog # 11789013, 11791019) and Infusion EcoDry kits (Clonetech, catalog # 638912), Coding sequences of tagYFP , tagGFP2 , tagRFP-T , ebfp2 , tagBFP , hupA , minDE , and aph frt were respectively amplified from the pTagYFP-C1, pTagGFP2-C1, pTagRFP-T, pBAD-EBFP, pTagBFP-C1, W3110 genome, W3110 genome, and pKD13, and inserted into the Gateway entry vectors through BP reactions as described in the Gateway protocol. ..

    Transfection:

    Article Title: Fibroblast activation protein (FAP) as a novel metabolic target
    Article Snippet: .. Amplified LIC-Sumo vector and FGF21 gene fragment were mixed together, treated using In-Fusion HD EcoDry Cloning Plus systems kit and transfected into stellar E. coli competent cells (both Clontech). .. Positive clones were selected on ampicillin/LB agar plate with single colonies picked to amplify plasmids with QIA prep miniprep kit (Qiagen).

    Article Title: Activity of the HIV-1 Attachment Inhibitor BMS-626529, the Active Component of the Prodrug BMS-663068, against CD4-Independent Viruses and HIV-1 Envelopes Resistant to Other Entry Inhibitors
    Article Snippet: .. Briefly, env clones were amplified by reverse transcriptase PCR (RT-PCR) and verified by sequencing, and the PCR products were cloned into the pCMV-HA expression vector (Clontech) by using In-Fusion HD EcoDry technology (Clontech) and transfected into cells to create effector cells. .. Cells were transfected with equal amounts of envelope-expressing plasmid DNA (50 ng/well in 6-well plates).

    Amplification:

    Article Title: Fibroblast activation protein (FAP) as a novel metabolic target
    Article Snippet: .. Amplified LIC-Sumo vector and FGF21 gene fragment were mixed together, treated using In-Fusion HD EcoDry Cloning Plus systems kit and transfected into stellar E. coli competent cells (both Clontech). .. Positive clones were selected on ampicillin/LB agar plate with single colonies picked to amplify plasmids with QIA prep miniprep kit (Qiagen).

    Article Title: Activity of the HIV-1 Attachment Inhibitor BMS-626529, the Active Component of the Prodrug BMS-663068, against CD4-Independent Viruses and HIV-1 Envelopes Resistant to Other Entry Inhibitors
    Article Snippet: .. Briefly, env clones were amplified by reverse transcriptase PCR (RT-PCR) and verified by sequencing, and the PCR products were cloned into the pCMV-HA expression vector (Clontech) by using In-Fusion HD EcoDry technology (Clontech) and transfected into cells to create effector cells. .. Cells were transfected with equal amounts of envelope-expressing plasmid DNA (50 ng/well in 6-well plates).

    Article Title: PRMT1-mediated methylation of the microprocessor-associated proteins regulates microRNA biogenesis
    Article Snippet: .. To overexpress PRMT1, the cDNA of the v2 isoform was amplified starting from HeLa cDNA, using the following primers: FW 5′-GGGGATCCCCCGGGCTGCAGATGGCGGCAGCCGAGGCCGCGAACTGCA-3′ REV 5′-GAGGTTGATTGTCGACTCAGCGCATCCGGTAGTCGGTGG-3′ The cDNA of PRMT1 was cloned into the pCCLsin.CPPT.PGK.GFP.WPRE lentiviral vector after plasmid linearization with PstI and SalI using the in-fusion HD EcoDry cloning plus, according to the manufacturer's instructions (Clontech Laboratories, Inc., CA, USA). .. The GFP cassette was removed from the vector upon digestion with XhoI.

    Article Title: Multi-color imaging of the bacterial nucleoid and division proteins with blue, orange, and near-infrared fluorescent proteins
    Article Snippet: .. Plasmid and Strain Construction The plasmids were constructed using Gateway cloning kit (Invitrogen, catalog # 11789013, 11791019) and Infusion EcoDry kits (Clonetech, catalog # 638912), Coding sequences of tagYFP , tagGFP2 , tagRFP-T , ebfp2 , tagBFP , hupA , minDE , and aph frt were respectively amplified from the pTagYFP-C1, pTagGFP2-C1, pTagRFP-T, pBAD-EBFP, pTagBFP-C1, W3110 genome, W3110 genome, and pKD13, and inserted into the Gateway entry vectors through BP reactions as described in the Gateway protocol. ..

    Polymerase Chain Reaction:

    Article Title: Activity of the HIV-1 Attachment Inhibitor BMS-626529, the Active Component of the Prodrug BMS-663068, against CD4-Independent Viruses and HIV-1 Envelopes Resistant to Other Entry Inhibitors
    Article Snippet: .. Briefly, env clones were amplified by reverse transcriptase PCR (RT-PCR) and verified by sequencing, and the PCR products were cloned into the pCMV-HA expression vector (Clontech) by using In-Fusion HD EcoDry technology (Clontech) and transfected into cells to create effector cells. .. Cells were transfected with equal amounts of envelope-expressing plasmid DNA (50 ng/well in 6-well plates).

    Construct:

    Article Title: C11orf95-RELA fusions drive oncogenic NF-κB signaling in ependymoma
    Article Snippet: .. The Clontech In-Fusion HD EcoDry Cloning Plus system was used to generate fusion constructs. .. All constructs were verified by sequencing and used to make retroviruses as described .

    Article Title: TRIOBP-5 sculpts stereocilia rootlets and stiffens supporting cells enabling hearing
    Article Snippet: .. The Triobp- 5Δcc expression vector in which the coiled-coil domains were deleted was constructed using 3 fragments from the full-length Triobp-5 cDNA that were combined using In-Fusion cloning (Takara, 638912), such that sequences encoded by exons 11, 12, and 15–22 were deleted. .. For transfection into HeLa cells in 6-well dishes, we used expression vectors (equal molar amounts for a total of 2.0 μg of DNA) that separately express AcGFP1-TRIOBP-5, DsRed-TRIOBP-5, and the MYO10NANOTRAP per well.

    Article Title: Multi-color imaging of the bacterial nucleoid and division proteins with blue, orange, and near-infrared fluorescent proteins
    Article Snippet: .. Plasmid and Strain Construction The plasmids were constructed using Gateway cloning kit (Invitrogen, catalog # 11789013, 11791019) and Infusion EcoDry kits (Clonetech, catalog # 638912), Coding sequences of tagYFP , tagGFP2 , tagRFP-T , ebfp2 , tagBFP , hupA , minDE , and aph frt were respectively amplified from the pTagYFP-C1, pTagGFP2-C1, pTagRFP-T, pBAD-EBFP, pTagBFP-C1, W3110 genome, W3110 genome, and pKD13, and inserted into the Gateway entry vectors through BP reactions as described in the Gateway protocol. ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Activity of the HIV-1 Attachment Inhibitor BMS-626529, the Active Component of the Prodrug BMS-663068, against CD4-Independent Viruses and HIV-1 Envelopes Resistant to Other Entry Inhibitors
    Article Snippet: .. Briefly, env clones were amplified by reverse transcriptase PCR (RT-PCR) and verified by sequencing, and the PCR products were cloned into the pCMV-HA expression vector (Clontech) by using In-Fusion HD EcoDry technology (Clontech) and transfected into cells to create effector cells. .. Cells were transfected with equal amounts of envelope-expressing plasmid DNA (50 ng/well in 6-well plates).

    Expressing:

    Article Title: Discovery and Characterization of a Novel CD4-Binding Adnectin with Potent Anti-HIV Activity
    Article Snippet: .. The envelope genes were cloned into the pCMV-HA expression vector (Clontech) using the In-Fusion HD EcoDry technology (Clontech) and were used in the assay to evaluate a wide spectrum of subtypes. ..

    Article Title: TRIOBP-5 sculpts stereocilia rootlets and stiffens supporting cells enabling hearing
    Article Snippet: .. The Triobp- 5Δcc expression vector in which the coiled-coil domains were deleted was constructed using 3 fragments from the full-length Triobp-5 cDNA that were combined using In-Fusion cloning (Takara, 638912), such that sequences encoded by exons 11, 12, and 15–22 were deleted. .. For transfection into HeLa cells in 6-well dishes, we used expression vectors (equal molar amounts for a total of 2.0 μg of DNA) that separately express AcGFP1-TRIOBP-5, DsRed-TRIOBP-5, and the MYO10NANOTRAP per well.

    Article Title: Activity of the HIV-1 Attachment Inhibitor BMS-626529, the Active Component of the Prodrug BMS-663068, against CD4-Independent Viruses and HIV-1 Envelopes Resistant to Other Entry Inhibitors
    Article Snippet: .. Briefly, env clones were amplified by reverse transcriptase PCR (RT-PCR) and verified by sequencing, and the PCR products were cloned into the pCMV-HA expression vector (Clontech) by using In-Fusion HD EcoDry technology (Clontech) and transfected into cells to create effector cells. .. Cells were transfected with equal amounts of envelope-expressing plasmid DNA (50 ng/well in 6-well plates).

    Sequencing:

    Article Title: Activity of the HIV-1 Attachment Inhibitor BMS-626529, the Active Component of the Prodrug BMS-663068, against CD4-Independent Viruses and HIV-1 Envelopes Resistant to Other Entry Inhibitors
    Article Snippet: .. Briefly, env clones were amplified by reverse transcriptase PCR (RT-PCR) and verified by sequencing, and the PCR products were cloned into the pCMV-HA expression vector (Clontech) by using In-Fusion HD EcoDry technology (Clontech) and transfected into cells to create effector cells. .. Cells were transfected with equal amounts of envelope-expressing plasmid DNA (50 ng/well in 6-well plates).

    Plasmid Preparation:

    Article Title: Discovery and Characterization of a Novel CD4-Binding Adnectin with Potent Anti-HIV Activity
    Article Snippet: .. The envelope genes were cloned into the pCMV-HA expression vector (Clontech) using the In-Fusion HD EcoDry technology (Clontech) and were used in the assay to evaluate a wide spectrum of subtypes. ..

    Article Title: TRIOBP-5 sculpts stereocilia rootlets and stiffens supporting cells enabling hearing
    Article Snippet: .. The Triobp- 5Δcc expression vector in which the coiled-coil domains were deleted was constructed using 3 fragments from the full-length Triobp-5 cDNA that were combined using In-Fusion cloning (Takara, 638912), such that sequences encoded by exons 11, 12, and 15–22 were deleted. .. For transfection into HeLa cells in 6-well dishes, we used expression vectors (equal molar amounts for a total of 2.0 μg of DNA) that separately express AcGFP1-TRIOBP-5, DsRed-TRIOBP-5, and the MYO10NANOTRAP per well.

    Article Title: Fibroblast activation protein (FAP) as a novel metabolic target
    Article Snippet: .. Amplified LIC-Sumo vector and FGF21 gene fragment were mixed together, treated using In-Fusion HD EcoDry Cloning Plus systems kit and transfected into stellar E. coli competent cells (both Clontech). .. Positive clones were selected on ampicillin/LB agar plate with single colonies picked to amplify plasmids with QIA prep miniprep kit (Qiagen).

    Article Title: Activity of the HIV-1 Attachment Inhibitor BMS-626529, the Active Component of the Prodrug BMS-663068, against CD4-Independent Viruses and HIV-1 Envelopes Resistant to Other Entry Inhibitors
    Article Snippet: .. Briefly, env clones were amplified by reverse transcriptase PCR (RT-PCR) and verified by sequencing, and the PCR products were cloned into the pCMV-HA expression vector (Clontech) by using In-Fusion HD EcoDry technology (Clontech) and transfected into cells to create effector cells. .. Cells were transfected with equal amounts of envelope-expressing plasmid DNA (50 ng/well in 6-well plates).

    Article Title: Promotion of Axon Growth by the Secreted End of a Transcription Factor
    Article Snippet: .. First, the open reading frame (ORF) of CREB3L2 was subcloned into a vector containing GFP (Takara, pEGFP-C1) using the In Fusion HD Cloning Plus kit (Takara, 638913). .. Afterward, the ORF for mCherry (Takara, pmCherry-C1, 632524) was amplified and inserted into the GFP-CREB3L2 construct using the In Fusion kit.

    Article Title: PRMT1-mediated methylation of the microprocessor-associated proteins regulates microRNA biogenesis
    Article Snippet: .. To overexpress PRMT1, the cDNA of the v2 isoform was amplified starting from HeLa cDNA, using the following primers: FW 5′-GGGGATCCCCCGGGCTGCAGATGGCGGCAGCCGAGGCCGCGAACTGCA-3′ REV 5′-GAGGTTGATTGTCGACTCAGCGCATCCGGTAGTCGGTGG-3′ The cDNA of PRMT1 was cloned into the pCCLsin.CPPT.PGK.GFP.WPRE lentiviral vector after plasmid linearization with PstI and SalI using the in-fusion HD EcoDry cloning plus, according to the manufacturer's instructions (Clontech Laboratories, Inc., CA, USA). .. The GFP cassette was removed from the vector upon digestion with XhoI.

    Article Title: Multi-color imaging of the bacterial nucleoid and division proteins with blue, orange, and near-infrared fluorescent proteins
    Article Snippet: .. Plasmid and Strain Construction The plasmids were constructed using Gateway cloning kit (Invitrogen, catalog # 11789013, 11791019) and Infusion EcoDry kits (Clonetech, catalog # 638912), Coding sequences of tagYFP , tagGFP2 , tagRFP-T , ebfp2 , tagBFP , hupA , minDE , and aph frt were respectively amplified from the pTagYFP-C1, pTagGFP2-C1, pTagRFP-T, pBAD-EBFP, pTagBFP-C1, W3110 genome, W3110 genome, and pKD13, and inserted into the Gateway entry vectors through BP reactions as described in the Gateway protocol. ..

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  • About
  • News
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  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    TaKaRa in fusion hd ecodry cloning plus systems kit
    In Fusion Hd Ecodry Cloning Plus Systems Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/in fusion hd ecodry cloning plus systems kit/product/TaKaRa
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    in fusion hd ecodry cloning plus systems kit - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    94
    TaKaRa in fusion hd plus ecodry cloning system
    In Fusion Hd Plus Ecodry Cloning System, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/in fusion hd plus ecodry cloning system/product/TaKaRa
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    in fusion hd plus ecodry cloning system - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

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