immunopure streptavidin horseradish peroxidase  (Thermo Fisher)


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    Structured Review

    Thermo Fisher immunopure streptavidin horseradish peroxidase
    Immunopure Streptavidin Horseradish Peroxidase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunopure streptavidin horseradish peroxidase/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immunopure streptavidin horseradish peroxidase - by Bioz Stars, 2020-04
    86/100 stars

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    Blocking Assay:

    Article Title: Electrophilic Affibodies Forming Covalent Bonds to Protein Targets *
    Article Snippet: After 1 h at 25 °C on a rocker, plates were washed with PBSTM for 4 × 8 min and once quickly with PBST, and 200 μl 1 μg/ml ImmunoPure streptavidin horseradish peroxidase (Pierce) diluted in PBS with 1% BSA was added for 1.5 h at 25 °C on a rocker. .. For D , after plating and blocking as above, samples were washed with PBSTM 2× and then with PBST 2× for 5 min at 25 °C.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Human Herpesvirus 8 Envelope Glycoprotein B Mediates Cell Adhesion via Its RGD Sequence
    Article Snippet: Paragraph title: ELISA. ... Plates were washed and incubated with ImmunoPure streptavidin horseradish peroxidase (0.1 μg/ml, 100 μl per well; Pierce, Rockford, Ill.) for 1 h at room temperature, and washed and bound horseradish peroxidase was quantified by reaction with 5-bromo-4-chloro-3-indolylphosphate-nitroblue tetrazolium substrate.

    Article Title: Electrophilic Affibodies Forming Covalent Bonds to Protein Targets *
    Article Snippet: Paragraph title: ELISA ... After 1 h at 25 °C on a rocker, plates were washed with PBSTM for 4 × 8 min and once quickly with PBST, and 200 μl 1 μg/ml ImmunoPure streptavidin horseradish peroxidase (Pierce) diluted in PBS with 1% BSA was added for 1.5 h at 25 °C on a rocker.

    Immunoprecipitation:

    Article Title: DISRUPTION OF LIPID RAFTS INHIBITS P2X1 RECEPTOR MEDIATED CURRENTS AND ARTERIAL VASOCONSTRICTION.
    Article Snippet: Cells from two 80cm2 tissue flasks were processed for membrane fractionation as described above and blotted with Immunopure streptavidin/horseradish peroxidase conjugated (0.5 μg/mL) (Pierce, Perbio Science, Tattenhall, UK). .. Cells contained in a 35 mm Ø Petri dish were processed for immunoprecipitation ( ).

    Incubation:

    Article Title: Human Herpesvirus 8 Envelope Glycoprotein B Mediates Cell Adhesion via Its RGD Sequence
    Article Snippet: .. Plates were washed and incubated with ImmunoPure streptavidin horseradish peroxidase (0.1 μg/ml, 100 μl per well; Pierce, Rockford, Ill.) for 1 h at room temperature, and washed and bound horseradish peroxidase was quantified by reaction with 5-bromo-4-chloro-3-indolylphosphate-nitroblue tetrazolium substrate. .. Cells in chamber slides were serum starved for 24 h at 37°C and were incubated with 200 μl of gBΔTM and gBΔTM RGA proteins (50 ng/ml) or LPA (20 ng/ml) in serum-free DMEM for 5 min at 37°C.

    other:

    Article Title: Biochemical characterization of a novel high-affinity and specific plasma kallikrein inhibitor
    Article Snippet: ImmunoPure streptavidin horseradish peroxidase and peroxide specific fast-reacting substrate, turbo-TMB were obtained from Pierce.

    Fractionation:

    Article Title: DISRUPTION OF LIPID RAFTS INHIBITS P2X1 RECEPTOR MEDIATED CURRENTS AND ARTERIAL VASOCONSTRICTION.
    Article Snippet: .. Cells from two 80cm2 tissue flasks were processed for membrane fractionation as described above and blotted with Immunopure streptavidin/horseradish peroxidase conjugated (0.5 μg/mL) (Pierce, Perbio Science, Tattenhall, UK). .. Cells contained in a 35 mm Ø Petri dish were processed for immunoprecipitation ( ).

    Expressing:

    Article Title: DISRUPTION OF LIPID RAFTS INHIBITS P2X1 RECEPTOR MEDIATED CURRENTS AND ARTERIAL VASOCONSTRICTION.
    Article Snippet: Paragraph title: Surface cell expression and immunoprecipitations ... Cells from two 80cm2 tissue flasks were processed for membrane fractionation as described above and blotted with Immunopure streptavidin/horseradish peroxidase conjugated (0.5 μg/mL) (Pierce, Perbio Science, Tattenhall, UK).

    Binding Assay:

    Article Title: Electrophilic Affibodies Forming Covalent Bonds to Protein Targets *
    Article Snippet: Proteins were coated at the indicated concentrations in 100 μl PBS with 10 m m dithiothreitol on Costar 9018 high capacity binding EIA/RIA polystyrene plates overnight at 4 °C. .. After 1 h at 25 °C on a rocker, plates were washed with PBSTM for 4 × 8 min and once quickly with PBST, and 200 μl 1 μg/ml ImmunoPure streptavidin horseradish peroxidase (Pierce) diluted in PBS with 1% BSA was added for 1.5 h at 25 °C on a rocker.

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    Thermo Fisher immunopurer avidin horseradish peroxidase
    Immunopurer Avidin Horseradish Peroxidase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunopurer avidin horseradish peroxidase/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immunopurer avidin horseradish peroxidase - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    86
    Thermo Fisher immunopure streptavidin hrp
    Immunopure Streptavidin Hrp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunopure streptavidin hrp/product/Thermo Fisher
    Average 86 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    immunopure streptavidin hrp - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

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