immunopure streptavidin horseradish peroxidase conjugate  (Thermo Fisher)


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    Thermo Fisher immunopure streptavidin horseradish peroxidase conjugate
    Immunopure Streptavidin Horseradish Peroxidase Conjugate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunopure streptavidin horseradish peroxidase conjugate/product/Thermo Fisher
    Average 79 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    immunopure streptavidin horseradish peroxidase conjugate - by Bioz Stars, 2020-03
    79/100 stars

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    Incubation:

    Article Title: Identification of cytokeratin 1 as a binding protein and presentation receptor for kininogens on endothelial cells
    Article Snippet: .. After washing, the bound biotin–HK was detected by incubation with a 1/500 dilution of ImmunoPure streptavidin horseradish peroxidase conjugate (Pierce) for 1 h followed by washing and the addition of 3,3′,5,5′-tetramethylbenzidine dihydrochloride peroxidase substrate (Pierce). .. Nonspecific binding was determined by the level of binding seen in the presence of a 50-fold molar excess unlabeled HK in the presence of Zn2+ .

    Article Title: Identification of cytokeratin 1 as a binding protein and presentation receptor for kininogens on endothelial cells
    Article Snippet: The cells were incubated with 20 nM biotin–HK in Hepes–Tyrode’s buffer containing 50 μM Zn2+ at 37°C for 1 h to achieve equilibrium. .. Cell-associated biotin–HK was detected by using ImmunoPure streptavidin horseradish peroxidase conjugate (Pierce) and fast-acting 3,3′,5,5′-tetramethylbenzidine dihydrochloride (TMB-Turbo) peroxidase substrate (Pierce) as reported ( , , , ).

    Binding Assay:

    Article Title: Identification of cytokeratin 1 as a binding protein and presentation receptor for kininogens on endothelial cells
    Article Snippet: Paragraph title: Binding of Biotin–HK to Cytokeratin. ... After washing, the bound biotin–HK was detected by incubation with a 1/500 dilution of ImmunoPure streptavidin horseradish peroxidase conjugate (Pierce) for 1 h followed by washing and the addition of 3,3′,5,5′-tetramethylbenzidine dihydrochloride peroxidase substrate (Pierce).

    Blocking Assay:

    Article Title: Identification of cytokeratin 1 as a binding protein and presentation receptor for kininogens on endothelial cells
    Article Snippet: After blocking the wells with 1% BSA for 1 h at 37°C, the wells were washed with 0.01 M sodium phosphate and 0.15 M NaCl (pH 7.4) followed by incubation of biotin–HK (20 nM) in the same buffer containing 1% BSA and 0.01% Tween 20 in the presence or absence of 50 μM Zn2+ with or without competitors for 1 h at 37°C. .. After washing, the bound biotin–HK was detected by incubation with a 1/500 dilution of ImmunoPure streptavidin horseradish peroxidase conjugate (Pierce) for 1 h followed by washing and the addition of 3,3′,5,5′-tetramethylbenzidine dihydrochloride peroxidase substrate (Pierce).

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  • 79
    Thermo Fisher immunopurer avidin horseradish peroxidase
    Immunopurer Avidin Horseradish Peroxidase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunopurer avidin horseradish peroxidase/product/Thermo Fisher
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immunopurer avidin horseradish peroxidase - by Bioz Stars, 2020-03
    79/100 stars
      Buy from Supplier

    80
    Thermo Fisher immunopure streptavidin hrp
    Immunopure Streptavidin Hrp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunopure streptavidin hrp/product/Thermo Fisher
    Average 80 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    immunopure streptavidin hrp - by Bioz Stars, 2020-03
    80/100 stars
      Buy from Supplier

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