Review




Structured Review

Servicebio Inc immunofluorescence staining
Bilayer hydrogel orchestrates inflammatory cell dynamics during the early inflammation phase of diabetic wound healing. a Experimental timeline for assay of early neutrophil recruitment. b Immunohistochemical staining for Ly-6G in wounds at 8 h, 1 d and 3 d after injury. Diabetic wounds were treated with SP/IL-10@Bilayer, SP@Bilayer, IL-10@Bilayer, and saline solution (Model), respectively. Healthy mice treated with saline solution were set as Normal. c Quantitative analysis of Ly-6G + cells in each group. d Relative expression of CXCL-1 on day 1. e Relative expression of MCP-1 on day 1. f Experimental timeline for assay of M1 macrophage infiltration. g <t>Immunofluorescence</t> staining for iNOS in wounds on days 1, 3 and 6 after injury. h Quantitative analysis of iNOS + cells in each group. i-k Relative expressions of macrophage-associated pro-inflammatory cytokines including TNF-α, IL-1β and IL-6 on day 3. l Schematic illustrating the dynamic modulation of inflammatory cells during the early inflammation phase of diabetic wounds by SP/IL-10@Bilayer. All data were generated from at least three independent experiments and presented as the means ± standard deviation. Statistical analysis was performed by one-way ANOVA. # means significant difference compared to the normal group. #p < 0.05, ##p < 0.01 and ###p < 0.001; ∗ means significant difference compared to the model group. ∗p < 0.05; & means significant difference compared to SP/IL-10@Bilayer. & p < 0.05 and && p < 0.01.
Immunofluorescence Staining, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunofluorescence+staining/immunofluorescence+staining/pmc12926990-310-14-20
Average 86 stars, based on 1 article reviews
immunofluorescence staining - by Bioz Stars, 2026-09
86/100 stars

Images

1) Product Images from "Skin-mimetic bilayer hydrogel normalizes diabetic wound healing by orchestrating inflammatory cell dynamics: An early intervention strategy"

Article Title: Skin-mimetic bilayer hydrogel normalizes diabetic wound healing by orchestrating inflammatory cell dynamics: An early intervention strategy

Journal: Bioactive Materials

doi: 10.1016/j.bioactmat.2026.02.025

Bilayer hydrogel orchestrates inflammatory cell dynamics during the early inflammation phase of diabetic wound healing. a Experimental timeline for assay of early neutrophil recruitment. b Immunohistochemical staining for Ly-6G in wounds at 8 h, 1 d and 3 d after injury. Diabetic wounds were treated with SP/IL-10@Bilayer, SP@Bilayer, IL-10@Bilayer, and saline solution (Model), respectively. Healthy mice treated with saline solution were set as Normal. c Quantitative analysis of Ly-6G + cells in each group. d Relative expression of CXCL-1 on day 1. e Relative expression of MCP-1 on day 1. f Experimental timeline for assay of M1 macrophage infiltration. g Immunofluorescence staining for iNOS in wounds on days 1, 3 and 6 after injury. h Quantitative analysis of iNOS + cells in each group. i-k Relative expressions of macrophage-associated pro-inflammatory cytokines including TNF-α, IL-1β and IL-6 on day 3. l Schematic illustrating the dynamic modulation of inflammatory cells during the early inflammation phase of diabetic wounds by SP/IL-10@Bilayer. All data were generated from at least three independent experiments and presented as the means ± standard deviation. Statistical analysis was performed by one-way ANOVA. # means significant difference compared to the normal group. #p < 0.05, ##p < 0.01 and ###p < 0.001; ∗ means significant difference compared to the model group. ∗p < 0.05; & means significant difference compared to SP/IL-10@Bilayer. & p < 0.05 and && p < 0.01.
Figure Legend Snippet: Bilayer hydrogel orchestrates inflammatory cell dynamics during the early inflammation phase of diabetic wound healing. a Experimental timeline for assay of early neutrophil recruitment. b Immunohistochemical staining for Ly-6G in wounds at 8 h, 1 d and 3 d after injury. Diabetic wounds were treated with SP/IL-10@Bilayer, SP@Bilayer, IL-10@Bilayer, and saline solution (Model), respectively. Healthy mice treated with saline solution were set as Normal. c Quantitative analysis of Ly-6G + cells in each group. d Relative expression of CXCL-1 on day 1. e Relative expression of MCP-1 on day 1. f Experimental timeline for assay of M1 macrophage infiltration. g Immunofluorescence staining for iNOS in wounds on days 1, 3 and 6 after injury. h Quantitative analysis of iNOS + cells in each group. i-k Relative expressions of macrophage-associated pro-inflammatory cytokines including TNF-α, IL-1β and IL-6 on day 3. l Schematic illustrating the dynamic modulation of inflammatory cells during the early inflammation phase of diabetic wounds by SP/IL-10@Bilayer. All data were generated from at least three independent experiments and presented as the means ± standard deviation. Statistical analysis was performed by one-way ANOVA. # means significant difference compared to the normal group. #p < 0.05, ##p < 0.01 and ###p < 0.001; ∗ means significant difference compared to the model group. ∗p < 0.05; & means significant difference compared to SP/IL-10@Bilayer. & p < 0.05 and && p < 0.01.

Techniques Used: Immunohistochemical staining, Staining, Saline, Expressing, Immunofluorescence, Generated, Standard Deviation

Bilayer hydrogel modulates M2c macrophage polarization in the later healing phase of diabetic wound. a Experimental timeline for assay of M2c macrophage polarization. b Immunofluorescence staining for CD163 in wounds on days 3, 6, 9 and 12 after injury. c Quantitative analysis of CD163 + cells in each group. d-f Relative expression of MerTK, IL-10, and TGF-β1 on day 6. g Schematic illustration of M2c macrophage polarization regulated by SP/IL-10@Bilayer and its contribution to inflammation resolution. All data were generated from three independent experiments and presented as the means ± standard deviation. Statistical analysis was performed by one-way ANOVA. # means significant difference compared to the normal group. #p < 0.05 and ##p < 0.01; ∗ means significant difference compared to model group. ∗p < 0.05, ∗∗p < 0.01 and ∗∗∗p < 0.001; & means significant difference compared to SP/IL-10@Bilayer group. & p < 0.05.
Figure Legend Snippet: Bilayer hydrogel modulates M2c macrophage polarization in the later healing phase of diabetic wound. a Experimental timeline for assay of M2c macrophage polarization. b Immunofluorescence staining for CD163 in wounds on days 3, 6, 9 and 12 after injury. c Quantitative analysis of CD163 + cells in each group. d-f Relative expression of MerTK, IL-10, and TGF-β1 on day 6. g Schematic illustration of M2c macrophage polarization regulated by SP/IL-10@Bilayer and its contribution to inflammation resolution. All data were generated from three independent experiments and presented as the means ± standard deviation. Statistical analysis was performed by one-way ANOVA. # means significant difference compared to the normal group. #p < 0.05 and ##p < 0.01; ∗ means significant difference compared to model group. ∗p < 0.05, ∗∗p < 0.01 and ∗∗∗p < 0.001; & means significant difference compared to SP/IL-10@Bilayer group. & p < 0.05.

Techniques Used: Immunofluorescence, Staining, Expressing, Generated, Standard Deviation

Related Articles

Immunofluorescence:

Article Title: Sensory nerve-derived semaphorin 3A promotes bone healing through macrophage immunomodulation.
Article Snippet: Sensory innervation has emerged as a crucial regulator of skeletal homeostasis and regeneration.. Growing evidence indicates that disruption of sensory nerves is associated with excessive inflammation and impaired tissue healing.. However, the underlying mechanisms by which neuronal signaling regulates the immune microenvironment to orchestrate bone repair remain unclear.

Article Title: Well-ST-seq: Cost-Effective and Near-Cellular Spatial Transcriptomics Using Deterministic Barcoded Bead Arrays.
Article Snippet: Spatial transcriptomic technologies are promising tools for elucidating fine anatomical profiles of tissues.. For methods that rely on deterministic probe arrays, balancing spatial resolution, cost, and transcript-capture sensitivity is crucial to advancing spatial transcriptomics in both basic research and clinical applications.. Here we present Well-ST-seq, a near-cellularresolution platform that integrates microwell-assembled hydrogel bead carriers with combinatorial microfluidic indexing to generate predefined spatial barcode arrays.

Article Title: An inhalable microbe-oncolytic virus consortium for lung cancer treatment
Article Snippet: .. On day 20, mice were euthanized, and lung tissues were harvested and fixed in 4% paraformaldehyde at 4°C for 24 h. Tissue paraffin embedding, sectioning, and immunofluorescence staining for CD4 + and CD8 + T cells were performed by Servicebio (Wuhan, China). ..

Article Title: Structure and Wound-Healing Activity of a Branched Levan-Type Fructan from Cyathula officinalis Roots.
Article Snippet: CD206 (Servicebio, GB113497) and CD68 (Thermo Fisher/eBioscience, clone FA-11, Cat. 14-0681-82) were used to assess macrophage-associated signals, with CD206 visualized in https://doi.org/10.3390/molecules31111981 the red channel and CD68 in the green channel. .. For single immunofluorescence staining, Ki67 (Servicebio, GB111499) was detected in the red channel. .. HIF-1α was detected using a FITC-conjugated antibody (Thermo Fisher, MA5-45251, clone ESEE122) and imaged in the green channel, with DAPI used as the nuclear counterstain.

Article Title: From clinical exosome analysis to engineered therapy: miR-21-5p-Enriched exosomes reverse thin endometrium via the YAP1 pathway.
Article Snippet: Thin endometrium (TE) is associated with reduced pregnancy rates and adverse obstetric outcomes.. While current interventions including hysteroscopic adhesiolysis and hormonal regimens offer partial solutions, functional restoration remains challenging due to the elusive pathogenesis of TE.. This work systematically decodes the miRNA landscape of uterine fluid-derived exosomes (UF-Exo) between TE patients and healthy women, uncovering a dramatic downregulation of miR-21-5p (74.0%) and miR-548aa (64.5%) in TE-derived UF-Exo that critically underpins disease progression.

Article Title: Skin-mimetic bilayer hydrogel normalizes diabetic wound healing by orchestrating inflammatory cell dynamics: An early intervention strategy
Article Snippet: Neutrophil recruitment was evaluated by immunohistochemical staining for Ly-6G (Servicebio, GB12229). .. The infiltration of pro-inflammatory (M1) macrophages and polarization of M2c macrophages were analyzed by immunofluorescence staining using antibodies against iNOS (Servicebio, GB11119) and CD163 (Servicebio, GB14027), respectively. ..

Article Title: An inhalable microbe-oncolytic virus consortium for lung cancer treatment.
Article Snippet: .. On day 20, mice were euthanized, and lung tissues were harvested and fixed in 4% paraformaldehyde at 4◦C for 24 h. Tissue paraffin embedding, sectioning, and immunofluorescence staining for CD4+ and CD8+ T cells were performed by Servicebio (Wuhan, China). ..

Staining:

Article Title: Sensory nerve-derived semaphorin 3A promotes bone healing through macrophage immunomodulation.
Article Snippet: Sensory innervation has emerged as a crucial regulator of skeletal homeostasis and regeneration.. Growing evidence indicates that disruption of sensory nerves is associated with excessive inflammation and impaired tissue healing.. However, the underlying mechanisms by which neuronal signaling regulates the immune microenvironment to orchestrate bone repair remain unclear.

Article Title: Well-ST-seq: Cost-Effective and Near-Cellular Spatial Transcriptomics Using Deterministic Barcoded Bead Arrays.
Article Snippet: Spatial transcriptomic technologies are promising tools for elucidating fine anatomical profiles of tissues.. For methods that rely on deterministic probe arrays, balancing spatial resolution, cost, and transcript-capture sensitivity is crucial to advancing spatial transcriptomics in both basic research and clinical applications.. Here we present Well-ST-seq, a near-cellularresolution platform that integrates microwell-assembled hydrogel bead carriers with combinatorial microfluidic indexing to generate predefined spatial barcode arrays.

Article Title: An inhalable microbe-oncolytic virus consortium for lung cancer treatment
Article Snippet: .. On day 20, mice were euthanized, and lung tissues were harvested and fixed in 4% paraformaldehyde at 4°C for 24 h. Tissue paraffin embedding, sectioning, and immunofluorescence staining for CD4 + and CD8 + T cells were performed by Servicebio (Wuhan, China). ..

Article Title: Structure and Wound-Healing Activity of a Branched Levan-Type Fructan from Cyathula officinalis Roots.
Article Snippet: CD206 (Servicebio, GB113497) and CD68 (Thermo Fisher/eBioscience, clone FA-11, Cat. 14-0681-82) were used to assess macrophage-associated signals, with CD206 visualized in https://doi.org/10.3390/molecules31111981 the red channel and CD68 in the green channel. .. For single immunofluorescence staining, Ki67 (Servicebio, GB111499) was detected in the red channel. .. HIF-1α was detected using a FITC-conjugated antibody (Thermo Fisher, MA5-45251, clone ESEE122) and imaged in the green channel, with DAPI used as the nuclear counterstain.

Article Title: From clinical exosome analysis to engineered therapy: miR-21-5p-Enriched exosomes reverse thin endometrium via the YAP1 pathway.
Article Snippet: Thin endometrium (TE) is associated with reduced pregnancy rates and adverse obstetric outcomes.. While current interventions including hysteroscopic adhesiolysis and hormonal regimens offer partial solutions, functional restoration remains challenging due to the elusive pathogenesis of TE.. This work systematically decodes the miRNA landscape of uterine fluid-derived exosomes (UF-Exo) between TE patients and healthy women, uncovering a dramatic downregulation of miR-21-5p (74.0%) and miR-548aa (64.5%) in TE-derived UF-Exo that critically underpins disease progression.

Article Title: Skin-mimetic bilayer hydrogel normalizes diabetic wound healing by orchestrating inflammatory cell dynamics: An early intervention strategy
Article Snippet: Neutrophil recruitment was evaluated by immunohistochemical staining for Ly-6G (Servicebio, GB12229). .. The infiltration of pro-inflammatory (M1) macrophages and polarization of M2c macrophages were analyzed by immunofluorescence staining using antibodies against iNOS (Servicebio, GB11119) and CD163 (Servicebio, GB14027), respectively. ..

Article Title: An inhalable microbe-oncolytic virus consortium for lung cancer treatment.
Article Snippet: .. On day 20, mice were euthanized, and lung tissues were harvested and fixed in 4% paraformaldehyde at 4◦C for 24 h. Tissue paraffin embedding, sectioning, and immunofluorescence staining for CD4+ and CD8+ T cells were performed by Servicebio (Wuhan, China). ..

Incubation:

Article Title: Sensory nerve-derived semaphorin 3A promotes bone healing through macrophage immunomodulation.
Article Snippet: Sensory innervation has emerged as a crucial regulator of skeletal homeostasis and regeneration.. Growing evidence indicates that disruption of sensory nerves is associated with excessive inflammation and impaired tissue healing.. However, the underlying mechanisms by which neuronal signaling regulates the immune microenvironment to orchestrate bone repair remain unclear.

Fluorescence In Situ Hybridization:

Article Title: Well-ST-seq: Cost-Effective and Near-Cellular Spatial Transcriptomics Using Deterministic Barcoded Bead Arrays.
Article Snippet: Spatial transcriptomic technologies are promising tools for elucidating fine anatomical profiles of tissues.. For methods that rely on deterministic probe arrays, balancing spatial resolution, cost, and transcript-capture sensitivity is crucial to advancing spatial transcriptomics in both basic research and clinical applications.. Here we present Well-ST-seq, a near-cellularresolution platform that integrates microwell-assembled hydrogel bead carriers with combinatorial microfluidic indexing to generate predefined spatial barcode arrays.

Double Immunofluorescence Staining:




Similar Products

99
Oxford Instruments immunofluorescence staining images
Immunofluorescence Staining Images, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunofluorescence+staining/Imaris/us12655088-635-1-7
Average 99 stars, based on 1 article reviews
immunofluorescence staining images - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier

86
Servicebio Inc immunofluorescence staining
Bilayer hydrogel orchestrates inflammatory cell dynamics during the early inflammation phase of diabetic wound healing. a Experimental timeline for assay of early neutrophil recruitment. b Immunohistochemical staining for Ly-6G in wounds at 8 h, 1 d and 3 d after injury. Diabetic wounds were treated with SP/IL-10@Bilayer, SP@Bilayer, IL-10@Bilayer, and saline solution (Model), respectively. Healthy mice treated with saline solution were set as Normal. c Quantitative analysis of Ly-6G + cells in each group. d Relative expression of CXCL-1 on day 1. e Relative expression of MCP-1 on day 1. f Experimental timeline for assay of M1 macrophage infiltration. g <t>Immunofluorescence</t> staining for iNOS in wounds on days 1, 3 and 6 after injury. h Quantitative analysis of iNOS + cells in each group. i-k Relative expressions of macrophage-associated pro-inflammatory cytokines including TNF-α, IL-1β and IL-6 on day 3. l Schematic illustrating the dynamic modulation of inflammatory cells during the early inflammation phase of diabetic wounds by SP/IL-10@Bilayer. All data were generated from at least three independent experiments and presented as the means ± standard deviation. Statistical analysis was performed by one-way ANOVA. # means significant difference compared to the normal group. #p < 0.05, ##p < 0.01 and ###p < 0.001; ∗ means significant difference compared to the model group. ∗p < 0.05; & means significant difference compared to SP/IL-10@Bilayer. & p < 0.05 and && p < 0.01.
Immunofluorescence Staining, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunofluorescence+staining/immunofluorescence+staining/pmc12926990-310-14-20
Average 86 stars, based on 1 article reviews
immunofluorescence staining - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

86
Servicebio Inc tunel immunofluorescence staining
Bilayer hydrogel orchestrates inflammatory cell dynamics during the early inflammation phase of diabetic wound healing. a Experimental timeline for assay of early neutrophil recruitment. b Immunohistochemical staining for Ly-6G in wounds at 8 h, 1 d and 3 d after injury. Diabetic wounds were treated with SP/IL-10@Bilayer, SP@Bilayer, IL-10@Bilayer, and saline solution (Model), respectively. Healthy mice treated with saline solution were set as Normal. c Quantitative analysis of Ly-6G + cells in each group. d Relative expression of CXCL-1 on day 1. e Relative expression of MCP-1 on day 1. f Experimental timeline for assay of M1 macrophage infiltration. g <t>Immunofluorescence</t> staining for iNOS in wounds on days 1, 3 and 6 after injury. h Quantitative analysis of iNOS + cells in each group. i-k Relative expressions of macrophage-associated pro-inflammatory cytokines including TNF-α, IL-1β and IL-6 on day 3. l Schematic illustrating the dynamic modulation of inflammatory cells during the early inflammation phase of diabetic wounds by SP/IL-10@Bilayer. All data were generated from at least three independent experiments and presented as the means ± standard deviation. Statistical analysis was performed by one-way ANOVA. # means significant difference compared to the normal group. #p < 0.05, ##p < 0.01 and ###p < 0.001; ∗ means significant difference compared to the model group. ∗p < 0.05; & means significant difference compared to SP/IL-10@Bilayer. & p < 0.05 and && p < 0.01.
Tunel Immunofluorescence Staining, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunofluorescence+staining/immunofluorescence+staining/pm42310753-392-16-20
Average 86 stars, based on 1 article reviews
tunel immunofluorescence staining - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

86
Human Protein Atlas immunofluorescence staining
Bilayer hydrogel orchestrates inflammatory cell dynamics during the early inflammation phase of diabetic wound healing. a Experimental timeline for assay of early neutrophil recruitment. b Immunohistochemical staining for Ly-6G in wounds at 8 h, 1 d and 3 d after injury. Diabetic wounds were treated with SP/IL-10@Bilayer, SP@Bilayer, IL-10@Bilayer, and saline solution (Model), respectively. Healthy mice treated with saline solution were set as Normal. c Quantitative analysis of Ly-6G + cells in each group. d Relative expression of CXCL-1 on day 1. e Relative expression of MCP-1 on day 1. f Experimental timeline for assay of M1 macrophage infiltration. g <t>Immunofluorescence</t> staining for iNOS in wounds on days 1, 3 and 6 after injury. h Quantitative analysis of iNOS + cells in each group. i-k Relative expressions of macrophage-associated pro-inflammatory cytokines including TNF-α, IL-1β and IL-6 on day 3. l Schematic illustrating the dynamic modulation of inflammatory cells during the early inflammation phase of diabetic wounds by SP/IL-10@Bilayer. All data were generated from at least three independent experiments and presented as the means ± standard deviation. Statistical analysis was performed by one-way ANOVA. # means significant difference compared to the normal group. #p < 0.05, ##p < 0.01 and ###p < 0.001; ∗ means significant difference compared to the model group. ∗p < 0.05; & means significant difference compared to SP/IL-10@Bilayer. & p < 0.05 and && p < 0.01.
Immunofluorescence Staining, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunofluorescence+staining/data+immunofluorescence/pm42287003-41-13-19
Average 86 stars, based on 1 article reviews
immunofluorescence staining - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

86
Human Protein Atlas immunofluorescence if staining
Bilayer hydrogel orchestrates inflammatory cell dynamics during the early inflammation phase of diabetic wound healing. a Experimental timeline for assay of early neutrophil recruitment. b Immunohistochemical staining for Ly-6G in wounds at 8 h, 1 d and 3 d after injury. Diabetic wounds were treated with SP/IL-10@Bilayer, SP@Bilayer, IL-10@Bilayer, and saline solution (Model), respectively. Healthy mice treated with saline solution were set as Normal. c Quantitative analysis of Ly-6G + cells in each group. d Relative expression of CXCL-1 on day 1. e Relative expression of MCP-1 on day 1. f Experimental timeline for assay of M1 macrophage infiltration. g <t>Immunofluorescence</t> staining for iNOS in wounds on days 1, 3 and 6 after injury. h Quantitative analysis of iNOS + cells in each group. i-k Relative expressions of macrophage-associated pro-inflammatory cytokines including TNF-α, IL-1β and IL-6 on day 3. l Schematic illustrating the dynamic modulation of inflammatory cells during the early inflammation phase of diabetic wounds by SP/IL-10@Bilayer. All data were generated from at least three independent experiments and presented as the means ± standard deviation. Statistical analysis was performed by one-way ANOVA. # means significant difference compared to the normal group. #p < 0.05, ##p < 0.01 and ###p < 0.001; ∗ means significant difference compared to the model group. ∗p < 0.05; & means significant difference compared to SP/IL-10@Bilayer. & p < 0.05 and && p < 0.01.
Immunofluorescence If Staining, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunofluorescence+staining/ihc+staining/pm42242683-97-55-64
Average 86 stars, based on 1 article reviews
immunofluorescence if staining - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

86
Fisher Scientific 263 immunofluorescent staining 264 sterile
Bilayer hydrogel orchestrates inflammatory cell dynamics during the early inflammation phase of diabetic wound healing. a Experimental timeline for assay of early neutrophil recruitment. b Immunohistochemical staining for Ly-6G in wounds at 8 h, 1 d and 3 d after injury. Diabetic wounds were treated with SP/IL-10@Bilayer, SP@Bilayer, IL-10@Bilayer, and saline solution (Model), respectively. Healthy mice treated with saline solution were set as Normal. c Quantitative analysis of Ly-6G + cells in each group. d Relative expression of CXCL-1 on day 1. e Relative expression of MCP-1 on day 1. f Experimental timeline for assay of M1 macrophage infiltration. g <t>Immunofluorescence</t> staining for iNOS in wounds on days 1, 3 and 6 after injury. h Quantitative analysis of iNOS + cells in each group. i-k Relative expressions of macrophage-associated pro-inflammatory cytokines including TNF-α, IL-1β and IL-6 on day 3. l Schematic illustrating the dynamic modulation of inflammatory cells during the early inflammation phase of diabetic wounds by SP/IL-10@Bilayer. All data were generated from at least three independent experiments and presented as the means ± standard deviation. Statistical analysis was performed by one-way ANOVA. # means significant difference compared to the normal group. #p < 0.05, ##p < 0.01 and ###p < 0.001; ∗ means significant difference compared to the model group. ∗p < 0.05; & means significant difference compared to SP/IL-10@Bilayer. & p < 0.05 and && p < 0.01.
263 Immunofluorescent Staining 264 Sterile, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunofluorescence+staining/263+264+immunofluorescent+staining+sterile/pm42250762-144-0-7
Average 86 stars, based on 1 article reviews
263 immunofluorescent staining 264 sterile - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

Image Search Results


Bilayer hydrogel orchestrates inflammatory cell dynamics during the early inflammation phase of diabetic wound healing. a Experimental timeline for assay of early neutrophil recruitment. b Immunohistochemical staining for Ly-6G in wounds at 8 h, 1 d and 3 d after injury. Diabetic wounds were treated with SP/IL-10@Bilayer, SP@Bilayer, IL-10@Bilayer, and saline solution (Model), respectively. Healthy mice treated with saline solution were set as Normal. c Quantitative analysis of Ly-6G + cells in each group. d Relative expression of CXCL-1 on day 1. e Relative expression of MCP-1 on day 1. f Experimental timeline for assay of M1 macrophage infiltration. g Immunofluorescence staining for iNOS in wounds on days 1, 3 and 6 after injury. h Quantitative analysis of iNOS + cells in each group. i-k Relative expressions of macrophage-associated pro-inflammatory cytokines including TNF-α, IL-1β and IL-6 on day 3. l Schematic illustrating the dynamic modulation of inflammatory cells during the early inflammation phase of diabetic wounds by SP/IL-10@Bilayer. All data were generated from at least three independent experiments and presented as the means ± standard deviation. Statistical analysis was performed by one-way ANOVA. # means significant difference compared to the normal group. #p < 0.05, ##p < 0.01 and ###p < 0.001; ∗ means significant difference compared to the model group. ∗p < 0.05; & means significant difference compared to SP/IL-10@Bilayer. & p < 0.05 and && p < 0.01.

Journal: Bioactive Materials

Article Title: Skin-mimetic bilayer hydrogel normalizes diabetic wound healing by orchestrating inflammatory cell dynamics: An early intervention strategy

doi: 10.1016/j.bioactmat.2026.02.025

Figure Lengend Snippet: Bilayer hydrogel orchestrates inflammatory cell dynamics during the early inflammation phase of diabetic wound healing. a Experimental timeline for assay of early neutrophil recruitment. b Immunohistochemical staining for Ly-6G in wounds at 8 h, 1 d and 3 d after injury. Diabetic wounds were treated with SP/IL-10@Bilayer, SP@Bilayer, IL-10@Bilayer, and saline solution (Model), respectively. Healthy mice treated with saline solution were set as Normal. c Quantitative analysis of Ly-6G + cells in each group. d Relative expression of CXCL-1 on day 1. e Relative expression of MCP-1 on day 1. f Experimental timeline for assay of M1 macrophage infiltration. g Immunofluorescence staining for iNOS in wounds on days 1, 3 and 6 after injury. h Quantitative analysis of iNOS + cells in each group. i-k Relative expressions of macrophage-associated pro-inflammatory cytokines including TNF-α, IL-1β and IL-6 on day 3. l Schematic illustrating the dynamic modulation of inflammatory cells during the early inflammation phase of diabetic wounds by SP/IL-10@Bilayer. All data were generated from at least three independent experiments and presented as the means ± standard deviation. Statistical analysis was performed by one-way ANOVA. # means significant difference compared to the normal group. #p < 0.05, ##p < 0.01 and ###p < 0.001; ∗ means significant difference compared to the model group. ∗p < 0.05; & means significant difference compared to SP/IL-10@Bilayer. & p < 0.05 and && p < 0.01.

Article Snippet: The infiltration of pro-inflammatory (M1) macrophages and polarization of M2c macrophages were analyzed by immunofluorescence staining using antibodies against iNOS (Servicebio, GB11119) and CD163 (Servicebio, GB14027), respectively.

Techniques: Immunohistochemical staining, Staining, Saline, Expressing, Immunofluorescence, Generated, Standard Deviation

Bilayer hydrogel modulates M2c macrophage polarization in the later healing phase of diabetic wound. a Experimental timeline for assay of M2c macrophage polarization. b Immunofluorescence staining for CD163 in wounds on days 3, 6, 9 and 12 after injury. c Quantitative analysis of CD163 + cells in each group. d-f Relative expression of MerTK, IL-10, and TGF-β1 on day 6. g Schematic illustration of M2c macrophage polarization regulated by SP/IL-10@Bilayer and its contribution to inflammation resolution. All data were generated from three independent experiments and presented as the means ± standard deviation. Statistical analysis was performed by one-way ANOVA. # means significant difference compared to the normal group. #p < 0.05 and ##p < 0.01; ∗ means significant difference compared to model group. ∗p < 0.05, ∗∗p < 0.01 and ∗∗∗p < 0.001; & means significant difference compared to SP/IL-10@Bilayer group. & p < 0.05.

Journal: Bioactive Materials

Article Title: Skin-mimetic bilayer hydrogel normalizes diabetic wound healing by orchestrating inflammatory cell dynamics: An early intervention strategy

doi: 10.1016/j.bioactmat.2026.02.025

Figure Lengend Snippet: Bilayer hydrogel modulates M2c macrophage polarization in the later healing phase of diabetic wound. a Experimental timeline for assay of M2c macrophage polarization. b Immunofluorescence staining for CD163 in wounds on days 3, 6, 9 and 12 after injury. c Quantitative analysis of CD163 + cells in each group. d-f Relative expression of MerTK, IL-10, and TGF-β1 on day 6. g Schematic illustration of M2c macrophage polarization regulated by SP/IL-10@Bilayer and its contribution to inflammation resolution. All data were generated from three independent experiments and presented as the means ± standard deviation. Statistical analysis was performed by one-way ANOVA. # means significant difference compared to the normal group. #p < 0.05 and ##p < 0.01; ∗ means significant difference compared to model group. ∗p < 0.05, ∗∗p < 0.01 and ∗∗∗p < 0.001; & means significant difference compared to SP/IL-10@Bilayer group. & p < 0.05.

Article Snippet: The infiltration of pro-inflammatory (M1) macrophages and polarization of M2c macrophages were analyzed by immunofluorescence staining using antibodies against iNOS (Servicebio, GB11119) and CD163 (Servicebio, GB14027), respectively.

Techniques: Immunofluorescence, Staining, Expressing, Generated, Standard Deviation