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Vicam immunoaffinity column
Immunoaffinity Column, supplied by Vicam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/immunoaffinity column/product/Vicam
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
immunoaffinity column - by Bioz Stars, 2021-03
86/100 stars

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Article Title: Detoxification of Aflatoxin-Contaminated Maize by Neutral Electrolyzed Oxidizing Water
Article Snippet: Aflatoxin Quantification Aflatoxin content was determined according to the 991.31 AOAC method [ ] using antibody-based immunoaffinity columns (IAC) for aflatoxin B1 and B2 (VICAM, Milford, MA, USA).

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Article Title: Incidence and Levels of Deoxynivalenol, Fumonisins and Zearalenone Contaminants in Animal Feeds Used in Korea in 2012
Article Snippet: .. The extract was then filtered through Whatman No. 6 filter paper, and 2.5 mL of filtered extract was applied to the immunoaffinity column (IAC; Vicam, DON test) containing specific antibodies for DON for purification. ..

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    Vicam immunoaffinity columns
    Multitoxin <t>Immunoaffinity</t> Column Cleanup and Liquid Chromatographic Quantitation First Action 2008
    Immunoaffinity Columns, supplied by Vicam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunoaffinity columns/product/Vicam
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immunoaffinity columns - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Vicam don niv
    <t>NIV,</t> <t>DON</t> and DON-3G concentrations (min, lower quartile, median, upper quartile, max) found in wheat sampled in six regions of Poland. Number of positive samples/total number of samples fractions are shown under bar charts.
    Don Niv, supplied by Vicam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/don niv/product/Vicam
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    don niv - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Vicam zearalatest column
    The determination of the recovery rate of the <t>ZearalaTest™</t> column and the determination of the standard curve for the ZEN toxin. ( T1 ): the ZEN standard curve. Under chromatographic conditions, linear regression analysis was carried out with the standard concentration (X) as the horizontal coordinate and the peak area (Y) as the vertical coordinate. ( T2 ): adsorption curve of the ZearalaTest™ column immunoaffinity column. ( T3 ): Recovery rate of ZearalaTest™ column immunoaffinity column. (A: The ZEN standard solution with 1 mL concentration of 1.5 μg/mL was used for direct determination. The chromatographic peak appeared at 9.521 min, and the peak area was 2.4. B: The standard solution of ZEN with 1 mL concentration of 1.5 μg/mL was recovered by the ZearalaTest™ column, and the recovered ZEN was dissolved in 1 mL methanol for HPLC detection. The chromatographic peak appeared at 9.191 min, and the peak area was 1.7.) The peak area was brought into the linear regression equation to calculate the sample content. Percent recovery (%) = adsorbing quantity/loading quantity.
    Zearalatest Column, supplied by Vicam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zearalatest column/product/Vicam
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    zearalatest column - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

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    Multitoxin Immunoaffinity Column Cleanup and Liquid Chromatographic Quantitation First Action 2008

    Journal:

    Article Title: Determination of Aflatoxins B1, B2, G1, and G2 and Ochratoxin A in Ginseng and Ginger by Multitoxin Immunoaffinity Column Cleanup and Liquid Chromatographic Quantitation: Collaborative Study

    doi:

    Figure Lengend Snippet: Multitoxin Immunoaffinity Column Cleanup and Liquid Chromatographic Quantitation First Action 2008

    Article Snippet: Three pairs of spiked ginger samples (total 6 test samples, 2 test samples/level) at 2, 4, and 16 μg/kg total AF (AFB1 :AFB2 :AFG1 :AFG2 = 4:1:2:1) and 1, 2, and 8 μg/kg OTA. ( 2 ) Practice samples.—Eight (2 ginseng test samples and 2 ginger test samples with known concentration of aflatoxins and OTA; and 2 ginseng test samples and 2 ginger test samples with unknown toxin concentrations). ( 3 ) Blank samples.—20 g blank ginseng and 20 g blank ginger (AF < 0.1 μg/kg, OTA < 0.1 μg/kg) for additional practice purposes, in case the participants were experiencing analytical problems with practice samples. ( 4 ) Immunoaffinity columns.—Forty AflaOchraTest immunoaffinity columns (Vicam, Watertown, MA). ( 5 ) Aflatoxin stock standard solution .—In a 4 mL silanized vial (total AF 400 ng/mL in acetonitrile: AFB1 200 ng/mL, AFB2 50 ng/mL, AFG1 100 ng/mL, AFG2 50 ng/mL). ( 6 ) OTA stock standard solution.—In a 4 mL silanized vial (OTA 200 ng/mL in methanol). ( 7 ) Dry phosphate buffer powder.—One tube.

    Techniques: Quantitation Assay

    Multitoxin Immunoaffinity Column Cleanup and Liquid Chromatographic Quantitation First Action 2008

    Journal:

    Article Title: Determination of Aflatoxins B1, B2, G1, and G2 and Ochratoxin A in Ginseng and Ginger by Multitoxin Immunoaffinity Column Cleanup and Liquid Chromatographic Quantitation: Collaborative Study

    doi:

    Figure Lengend Snippet: Multitoxin Immunoaffinity Column Cleanup and Liquid Chromatographic Quantitation First Action 2008

    Article Snippet: Three pairs of spiked ginger samples (total 6 test samples, 2 test samples/level) at 2, 4, and 16 μg/kg total AF (AFB1 :AFB2 :AFG1 :AFG2 = 4:1:2:1) and 1, 2, and 8 μg/kg OTA. ( 2 ) Practice samples.—Eight (2 ginseng test samples and 2 ginger test samples with known concentration of aflatoxins and OTA; and 2 ginseng test samples and 2 ginger test samples with unknown toxin concentrations). ( 3 ) Blank samples.—20 g blank ginseng and 20 g blank ginger (AF < 0.1 μg/kg, OTA < 0.1 μg/kg) for additional practice purposes, in case the participants were experiencing analytical problems with practice samples. ( 4 ) Immunoaffinity columns.—Forty AflaOchraTest immunoaffinity columns (Vicam, Watertown, MA). ( 5 ) Aflatoxin stock standard solution .—In a 4 mL silanized vial (total AF 400 ng/mL in acetonitrile: AFB1 200 ng/mL, AFB2 50 ng/mL, AFG1 100 ng/mL, AFG2 50 ng/mL). ( 6 ) OTA stock standard solution.—In a 4 mL silanized vial (OTA 200 ng/mL in methanol). ( 7 ) Dry phosphate buffer powder.—One tube.

    Techniques: Quantitation Assay

    NIV, DON and DON-3G concentrations (min, lower quartile, median, upper quartile, max) found in wheat sampled in six regions of Poland. Number of positive samples/total number of samples fractions are shown under bar charts.

    Journal: Toxins

    Article Title: Natural Occurrence of Nivalenol, Deoxynivalenol, and Deoxynivalenol-3-Glucoside in Polish Winter Wheat

    doi: 10.3390/toxins10020081

    Figure Lengend Snippet: NIV, DON and DON-3G concentrations (min, lower quartile, median, upper quartile, max) found in wheat sampled in six regions of Poland. Number of positive samples/total number of samples fractions are shown under bar charts.

    Article Snippet: Instruments, Warsaw, Poland) at 10,730 × g for 10 min. 3 mL of the extract dissolved with 3 mL of phosphate buffered saline (PBS) was again centrifuged at 10,730 × g for 10 min. 5 mL of the extract was passed through a DON-NIV (wide-bore, WB) immunoaffinity column (Vicam, Watertown, MA, USA) at a speed of 1–2 drops/s.

    Techniques:

    Black: chromatogram taken from a naturally contaminated wheat sample. Red: chromatogram of 480 µg/kg NIV, DON, and DON-3G standards.

    Journal: Toxins

    Article Title: Natural Occurrence of Nivalenol, Deoxynivalenol, and Deoxynivalenol-3-Glucoside in Polish Winter Wheat

    doi: 10.3390/toxins10020081

    Figure Lengend Snippet: Black: chromatogram taken from a naturally contaminated wheat sample. Red: chromatogram of 480 µg/kg NIV, DON, and DON-3G standards.

    Article Snippet: Instruments, Warsaw, Poland) at 10,730 × g for 10 min. 3 mL of the extract dissolved with 3 mL of phosphate buffered saline (PBS) was again centrifuged at 10,730 × g for 10 min. 5 mL of the extract was passed through a DON-NIV (wide-bore, WB) immunoaffinity column (Vicam, Watertown, MA, USA) at a speed of 1–2 drops/s.

    Techniques:

    The determination of the recovery rate of the ZearalaTest™ column and the determination of the standard curve for the ZEN toxin. ( T1 ): the ZEN standard curve. Under chromatographic conditions, linear regression analysis was carried out with the standard concentration (X) as the horizontal coordinate and the peak area (Y) as the vertical coordinate. ( T2 ): adsorption curve of the ZearalaTest™ column immunoaffinity column. ( T3 ): Recovery rate of ZearalaTest™ column immunoaffinity column. (A: The ZEN standard solution with 1 mL concentration of 1.5 μg/mL was used for direct determination. The chromatographic peak appeared at 9.521 min, and the peak area was 2.4. B: The standard solution of ZEN with 1 mL concentration of 1.5 μg/mL was recovered by the ZearalaTest™ column, and the recovered ZEN was dissolved in 1 mL methanol for HPLC detection. The chromatographic peak appeared at 9.191 min, and the peak area was 1.7.) The peak area was brought into the linear regression equation to calculate the sample content. Percent recovery (%) = adsorbing quantity/loading quantity.

    Journal: Toxins

    Article Title: The Protective Role of Bacillus velezensis A2 on the Biochemical and Hepatic Toxicity of Zearalenone in Mice

    doi: 10.3390/toxins10110449

    Figure Lengend Snippet: The determination of the recovery rate of the ZearalaTest™ column and the determination of the standard curve for the ZEN toxin. ( T1 ): the ZEN standard curve. Under chromatographic conditions, linear regression analysis was carried out with the standard concentration (X) as the horizontal coordinate and the peak area (Y) as the vertical coordinate. ( T2 ): adsorption curve of the ZearalaTest™ column immunoaffinity column. ( T3 ): Recovery rate of ZearalaTest™ column immunoaffinity column. (A: The ZEN standard solution with 1 mL concentration of 1.5 μg/mL was used for direct determination. The chromatographic peak appeared at 9.521 min, and the peak area was 2.4. B: The standard solution of ZEN with 1 mL concentration of 1.5 μg/mL was recovered by the ZearalaTest™ column, and the recovered ZEN was dissolved in 1 mL methanol for HPLC detection. The chromatographic peak appeared at 9.191 min, and the peak area was 1.7.) The peak area was brought into the linear regression equation to calculate the sample content. Percent recovery (%) = adsorbing quantity/loading quantity.

    Article Snippet: Because the ZearalaTest™ column (an immunoaffinity chromatographic column, VICAM, Milford, MA, USA.

    Techniques: Concentration Assay, Adsorption, High Performance Liquid Chromatography

    The determination of the recovery rate of the ZearalaTest™ column and the determination of the standard curve for the ZEN toxin. ( T1 ): the ZEN standard curve. Under chromatographic conditions, linear regression analysis was carried out with the standard concentration (X) as the horizontal coordinate and the peak area (Y) as the vertical coordinate. ( T2 ): adsorption curve of the ZearalaTest™ column immunoaffinity column. ( T3 ): Recovery rate of ZearalaTest™ column immunoaffinity column. (A: The ZEN standard solution with 1 mL concentration of 1.5 μg/mL was used for direct determination. The chromatographic peak appeared at 9.521 min, and the peak area was 2.4. B: The standard solution of ZEN with 1 mL concentration of 1.5 μg/mL was recovered by the ZearalaTest™ column, and the recovered ZEN was dissolved in 1 mL methanol for HPLC detection. The chromatographic peak appeared at 9.191 min, and the peak area was 1.7.) The peak area was brought into the linear regression equation to calculate the sample content. Percent recovery (%) = adsorbing quantity/loading quantity.

    Journal: Toxins

    Article Title: The Protective Role of Bacillus velezensis A2 on the Biochemical and Hepatic Toxicity of Zearalenone in Mice

    doi: 10.3390/toxins10110449

    Figure Lengend Snippet: The determination of the recovery rate of the ZearalaTest™ column and the determination of the standard curve for the ZEN toxin. ( T1 ): the ZEN standard curve. Under chromatographic conditions, linear regression analysis was carried out with the standard concentration (X) as the horizontal coordinate and the peak area (Y) as the vertical coordinate. ( T2 ): adsorption curve of the ZearalaTest™ column immunoaffinity column. ( T3 ): Recovery rate of ZearalaTest™ column immunoaffinity column. (A: The ZEN standard solution with 1 mL concentration of 1.5 μg/mL was used for direct determination. The chromatographic peak appeared at 9.521 min, and the peak area was 2.4. B: The standard solution of ZEN with 1 mL concentration of 1.5 μg/mL was recovered by the ZearalaTest™ column, and the recovered ZEN was dissolved in 1 mL methanol for HPLC detection. The chromatographic peak appeared at 9.191 min, and the peak area was 1.7.) The peak area was brought into the linear regression equation to calculate the sample content. Percent recovery (%) = adsorbing quantity/loading quantity.

    Article Snippet: Because the ZearalaTest™ column (an immunoaffinity chromatographic column, VICAM, Milford, MA, USA.

    Techniques: Concentration Assay, Adsorption, High Performance Liquid Chromatography