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R&D Systems c57bl 6 mice
Allogeneic MLR of bone marrow–derived DCs and splenocytes. Bone marrow cells were transduced by coculture with retroviral producer lines, CreLacZ (DC–β-gal) and CreNeu (DC–Neu), and differentiated into DCs in vitro. DCs were cocultured with allogeneic, <t>C57BL/6</t> T cells, isolated from bulk splenocytes by passing cells through an immunoaffinity column. After 3.5 d in culture, cells were pulsed with [ 3 H]thymidine as described in Materials and Methods. Results from triplicate wells were corrected for [ 3 H]thymidine incorporation by irradiated stimulators and T cells alone, and are plotted as the mean ± SEM.
C57bl 6 Mice, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 350 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/c57bl 6 mice/product/R&D Systems
Average 94 stars, based on 350 article reviews
Price from $9.99 to $1999.99
c57bl 6 mice - by Bioz Stars, 2020-09
94/100 stars

Images

1) Product Images from "Dendritic Cells Retrovirally Transduced with a Model Antigen Gene Are Therapeutically Effective against Established Pulmonary Metastases "

Article Title: Dendritic Cells Retrovirally Transduced with a Model Antigen Gene Are Therapeutically Effective against Established Pulmonary Metastases

Journal: The Journal of Experimental Medicine

doi:

Allogeneic MLR of bone marrow–derived DCs and splenocytes. Bone marrow cells were transduced by coculture with retroviral producer lines, CreLacZ (DC–β-gal) and CreNeu (DC–Neu), and differentiated into DCs in vitro. DCs were cocultured with allogeneic, C57BL/6 T cells, isolated from bulk splenocytes by passing cells through an immunoaffinity column. After 3.5 d in culture, cells were pulsed with [ 3 H]thymidine as described in Materials and Methods. Results from triplicate wells were corrected for [ 3 H]thymidine incorporation by irradiated stimulators and T cells alone, and are plotted as the mean ± SEM.
Figure Legend Snippet: Allogeneic MLR of bone marrow–derived DCs and splenocytes. Bone marrow cells were transduced by coculture with retroviral producer lines, CreLacZ (DC–β-gal) and CreNeu (DC–Neu), and differentiated into DCs in vitro. DCs were cocultured with allogeneic, C57BL/6 T cells, isolated from bulk splenocytes by passing cells through an immunoaffinity column. After 3.5 d in culture, cells were pulsed with [ 3 H]thymidine as described in Materials and Methods. Results from triplicate wells were corrected for [ 3 H]thymidine incorporation by irradiated stimulators and T cells alone, and are plotted as the mean ± SEM.

Techniques Used: Derivative Assay, In Vitro, Isolation, Irradiation

2) Product Images from "Dendritic Cells Retrovirally Transduced with a Model Antigen Gene Are Therapeutically Effective against Established Pulmonary Metastases "

Article Title: Dendritic Cells Retrovirally Transduced with a Model Antigen Gene Are Therapeutically Effective against Established Pulmonary Metastases

Journal: The Journal of Experimental Medicine

doi:

Allogeneic MLR of bone marrow–derived DCs and splenocytes. Bone marrow cells were transduced by coculture with retroviral producer lines, CreLacZ (DC–β-gal) and CreNeu (DC–Neu), and differentiated into DCs in vitro. DCs were cocultured with allogeneic, C57BL/6 T cells, isolated from bulk splenocytes by passing cells through an immunoaffinity column. After 3.5 d in culture, cells were pulsed with [ 3 H]thymidine as described in Materials and Methods. Results from triplicate wells were corrected for [ 3 H]thymidine incorporation by irradiated stimulators and T cells alone, and are plotted as the mean ± SEM.
Figure Legend Snippet: Allogeneic MLR of bone marrow–derived DCs and splenocytes. Bone marrow cells were transduced by coculture with retroviral producer lines, CreLacZ (DC–β-gal) and CreNeu (DC–Neu), and differentiated into DCs in vitro. DCs were cocultured with allogeneic, C57BL/6 T cells, isolated from bulk splenocytes by passing cells through an immunoaffinity column. After 3.5 d in culture, cells were pulsed with [ 3 H]thymidine as described in Materials and Methods. Results from triplicate wells were corrected for [ 3 H]thymidine incorporation by irradiated stimulators and T cells alone, and are plotted as the mean ± SEM.

Techniques Used: Derivative Assay, In Vitro, Isolation, Irradiation

3) Product Images from "C9orf72, a protein associated with amyotrophic lateral sclerosis (ALS) is a guanine nucleotide exchange factor"

Article Title: C9orf72, a protein associated with amyotrophic lateral sclerosis (ALS) is a guanine nucleotide exchange factor

Journal: PeerJ

doi: 10.7717/peerj.5815

Analysis of expression optimization of C9orf72 in Sf9 cells. pOPINF-C9orf72 construct selected for expression in Sf9 cells was tested for (A) total volume of virus used by SDS–PAGE analysis. The two series of volumes (1, 10 and 25 μl) shown represent volumes of viruses generated from using two different concentrations of DNA during transfection. (B) Total time of infection (3d, 3 days and 5d, 5 days). Expression was analyzed by western blotting as described in the materials and methods section. Detection was carried out using a monoclonal mouse anti-His (primary) antibody (1:5,000) from R D Systems (MAB050) in combination with an HRP-conjugated polyclonal mouse (secondary) antibody (1:10,000), raised in goat and Luminata™ Forte western HRP substrate. The lanes labeled M in both panels contain the PageRuler™ Plus Prestained Protein ladder (Fermentas). Molecular marker masses are indicated in kilodalton.
Figure Legend Snippet: Analysis of expression optimization of C9orf72 in Sf9 cells. pOPINF-C9orf72 construct selected for expression in Sf9 cells was tested for (A) total volume of virus used by SDS–PAGE analysis. The two series of volumes (1, 10 and 25 μl) shown represent volumes of viruses generated from using two different concentrations of DNA during transfection. (B) Total time of infection (3d, 3 days and 5d, 5 days). Expression was analyzed by western blotting as described in the materials and methods section. Detection was carried out using a monoclonal mouse anti-His (primary) antibody (1:5,000) from R D Systems (MAB050) in combination with an HRP-conjugated polyclonal mouse (secondary) antibody (1:10,000), raised in goat and Luminata™ Forte western HRP substrate. The lanes labeled M in both panels contain the PageRuler™ Plus Prestained Protein ladder (Fermentas). Molecular marker masses are indicated in kilodalton.

Techniques Used: Expressing, Construct, SDS Page, Generated, Transfection, Infection, Western Blot, Labeling, Marker

4) Product Images from "C9orf72, a protein associated with amyotrophic lateral sclerosis (ALS) is a guanine nucleotide exchange factor"

Article Title: C9orf72, a protein associated with amyotrophic lateral sclerosis (ALS) is a guanine nucleotide exchange factor

Journal: PeerJ

doi: 10.7717/peerj.5815

Analysis of expression optimization of C9orf72 in Sf9 cells. pOPINF-C9orf72 construct selected for expression in Sf9 cells was tested for (A) total volume of virus used by SDS–PAGE analysis. The two series of volumes (1, 10 and 25 μl) shown represent volumes of viruses generated from using two different concentrations of DNA during transfection. (B) Total time of infection (3d, 3 days and 5d, 5 days). Expression was analyzed by western blotting as described in the materials and methods section. Detection was carried out using a monoclonal mouse anti-His (primary) antibody (1:5,000) from R D Systems (MAB050) in combination with an HRP-conjugated polyclonal mouse (secondary) antibody (1:10,000), raised in goat and Luminata™ Forte western HRP substrate. The lanes labeled M in both panels contain the PageRuler™ Plus Prestained Protein ladder (Fermentas). Molecular marker masses are indicated in kilodalton.
Figure Legend Snippet: Analysis of expression optimization of C9orf72 in Sf9 cells. pOPINF-C9orf72 construct selected for expression in Sf9 cells was tested for (A) total volume of virus used by SDS–PAGE analysis. The two series of volumes (1, 10 and 25 μl) shown represent volumes of viruses generated from using two different concentrations of DNA during transfection. (B) Total time of infection (3d, 3 days and 5d, 5 days). Expression was analyzed by western blotting as described in the materials and methods section. Detection was carried out using a monoclonal mouse anti-His (primary) antibody (1:5,000) from R D Systems (MAB050) in combination with an HRP-conjugated polyclonal mouse (secondary) antibody (1:10,000), raised in goat and Luminata™ Forte western HRP substrate. The lanes labeled M in both panels contain the PageRuler™ Plus Prestained Protein ladder (Fermentas). Molecular marker masses are indicated in kilodalton.

Techniques Used: Expressing, Construct, SDS Page, Generated, Transfection, Infection, Western Blot, Labeling, Marker

Related Articles

Clone Assay:

Article Title: C9orf72, a protein associated with amyotrophic lateral sclerosis (ALS) is a guanine nucleotide exchange factor
Article Snippet: .. In-Fusion cloning kit (638916), monoclonal mouse anti-His (primary) antibody (MAB050) and HisTrap HP (five ml) column for affinity chromatography were sourced from R & D Systems (Minneapolis, MN, USA), Clontech (Takara Bio USA, Inc., Mountain View, CA, USA) and GE Healthcare (Buckinghamshire, UK), respectively. ..

Affinity Chromatography:

Article Title: C9orf72, a protein associated with amyotrophic lateral sclerosis (ALS) is a guanine nucleotide exchange factor
Article Snippet: .. In-Fusion cloning kit (638916), monoclonal mouse anti-His (primary) antibody (MAB050) and HisTrap HP (five ml) column for affinity chromatography were sourced from R & D Systems (Minneapolis, MN, USA), Clontech (Takara Bio USA, Inc., Mountain View, CA, USA) and GE Healthcare (Buckinghamshire, UK), respectively. ..

Labeling:

Article Title: Improving the developability of an anti-EphA2 single-chain variable fragment for nanoparticle targeting
Article Snippet: .. The antigen bound cells were then incubated with 2 μg/mL M2 anti-FLAG antibody (Sigma, F1804) labeled with Alexa647 (Life Technologies, A-30679) and 2 μg/mL anti-His6 antibody (R & D Systems, MAB050) labeled with Alexa 488 (Thermo Fisher Scientific, A-30676) for 30 minutes. .. After two washes, the cells were re-suspended in FACS buffer and the fluorescent signal was measured using a FACSCalibur (Becton Dickinson; Franklin Lakes, NJ).

Enzyme-linked Immunosorbent Assay:

Article Title: The HIV-1 Envelope Glycoprotein C3/V4 Region Defines a Prevalent Neutralization Epitope following Immunization
Article Snippet: .. For BG505 SOSIP.664 ELISA, mouse anti-His tag mAb (R & D Systems, MAB050) at 2 μg/ml in 100 μL of phosphate buffered saline (PBS) was coated at 4°C overnight. .. After incubating with blocking buffer for 1 hr at 37°C, 2 μg/ml of BG505 SOSIP.664 Env proteins were added into each well and incubated for 1 hr at room temperature.

Incubation:

Article Title: Improving the developability of an anti-EphA2 single-chain variable fragment for nanoparticle targeting
Article Snippet: .. The antigen bound cells were then incubated with 2 μg/mL M2 anti-FLAG antibody (Sigma, F1804) labeled with Alexa647 (Life Technologies, A-30679) and 2 μg/mL anti-His6 antibody (R & D Systems, MAB050) labeled with Alexa 488 (Thermo Fisher Scientific, A-30676) for 30 minutes. .. After two washes, the cells were re-suspended in FACS buffer and the fluorescent signal was measured using a FACSCalibur (Becton Dickinson; Franklin Lakes, NJ).

Western Blot:

Article Title: A novel immune resistance mechanism of melanoma cells controlled by the ADAR1 enzyme
Article Snippet: .. Western blotting WB using ADAR1 (Sigma-Aldrich, cat#HPA003890), polyHistidine (R & D Systems, cat#MAB050), and β-actin (MP Biomedicals, cat#0869100) antibodies was performed according to manufacturer's recommendations and as previously described [ ]. .. Determination of apoptosis 624mel ADAR1-p110 and Mock cells were stained with both annexin V–FITC and PI according to the manufacturer's instructions (eBioscience, cat#BMS500FI).

Chloramphenicol Acetyltransferase Assay:

Article Title: A novel immune resistance mechanism of melanoma cells controlled by the ADAR1 enzyme
Article Snippet: .. Western blotting WB using ADAR1 (Sigma-Aldrich, catHPA003890), polyHistidine (R & D Systems, cat#MAB050), and β-actin (MP Biomedicals, cat#0869100) antibodies was performed according to manufacturer's recommendations and as previously described [ ]. .. Determination of apoptosis 624mel ADAR1-p110 and Mock cells were stained with both annexin V–FITC and PI according to the manufacturer's instructions (eBioscience, cat#BMS500FI).

Chromatin Immunoprecipitation:

Article Title: Generation of a high-fidelity antibody against nerve growth factor using library scanning mutagenesis and validation with structures of the initial and optimized Fab-antigen complexes
Article Snippet: .. Surface preparation was the result of immobilization of mouse monoclonal anti-HIS onto CM5 sensor surface Anti-HIS surfaces were prepared by amine-coupling of a mouse monoclonal IgG1 anti-HIS tag antibody (R & D Systems; Catalog# MAB050) to a Biacore CM5 sensor chip surface at 25 °C on a Biacore 2000 SPR instrument. ..

SPR Assay:

Article Title: Generation of a high-fidelity antibody against nerve growth factor using library scanning mutagenesis and validation with structures of the initial and optimized Fab-antigen complexes
Article Snippet: .. Surface preparation was the result of immobilization of mouse monoclonal anti-HIS onto CM5 sensor surface Anti-HIS surfaces were prepared by amine-coupling of a mouse monoclonal IgG1 anti-HIS tag antibody (R & D Systems; Catalog# MAB050) to a Biacore CM5 sensor chip surface at 25 °C on a Biacore 2000 SPR instrument. ..

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    R&D Systems immunoaffinity column
    Allogeneic MLR of bone marrow–derived DCs and splenocytes. Bone marrow cells were transduced by coculture with retroviral producer lines, CreLacZ (DC–β-gal) and CreNeu (DC–Neu), and differentiated into DCs in vitro. DCs were cocultured with allogeneic, C57BL/6 T cells, isolated from bulk splenocytes by passing cells through an <t>immunoaffinity</t> column. After 3.5 d in culture, cells were pulsed with [ 3 H]thymidine as described in Materials and Methods. Results from triplicate wells were corrected for [ 3 H]thymidine incorporation by irradiated stimulators and T cells alone, and are plotted as the mean ± SEM.
    Immunoaffinity Column, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunoaffinity column/product/R&D Systems
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    immunoaffinity column - by Bioz Stars, 2020-09
    91/100 stars
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    Allogeneic MLR of bone marrow–derived DCs and splenocytes. Bone marrow cells were transduced by coculture with retroviral producer lines, CreLacZ (DC–β-gal) and CreNeu (DC–Neu), and differentiated into DCs in vitro. DCs were cocultured with allogeneic, C57BL/6 T cells, isolated from bulk splenocytes by passing cells through an immunoaffinity column. After 3.5 d in culture, cells were pulsed with [ 3 H]thymidine as described in Materials and Methods. Results from triplicate wells were corrected for [ 3 H]thymidine incorporation by irradiated stimulators and T cells alone, and are plotted as the mean ± SEM.

    Journal: The Journal of Experimental Medicine

    Article Title: Dendritic Cells Retrovirally Transduced with a Model Antigen Gene Are Therapeutically Effective against Established Pulmonary Metastases

    doi:

    Figure Lengend Snippet: Allogeneic MLR of bone marrow–derived DCs and splenocytes. Bone marrow cells were transduced by coculture with retroviral producer lines, CreLacZ (DC–β-gal) and CreNeu (DC–Neu), and differentiated into DCs in vitro. DCs were cocultured with allogeneic, C57BL/6 T cells, isolated from bulk splenocytes by passing cells through an immunoaffinity column. After 3.5 d in culture, cells were pulsed with [ 3 H]thymidine as described in Materials and Methods. Results from triplicate wells were corrected for [ 3 H]thymidine incorporation by irradiated stimulators and T cells alone, and are plotted as the mean ± SEM.

    Article Snippet: Bone marrow–derived DCs (transduced and nontransduced) or freshly prepared splenocytes were irradiated (2,000 rads) and plated in graded doses with 2 × 105 allogeneic T cells in 200 μl of RPMI-1640 media supplemented with 10% heat-inactivated fetal calf serum, 2 mmol/liter glutamine, 100 U/ml penicillin, 100 μg/ml streptomycin, 1× nonessential amino acids, 1 mM sodium pyruvate (all from Biofluids), 1.25 μg/ml amphotericin B (Fungizone; GIBCO BRL ), and 50 μg/ml gentamicin sulfate ( GIBCO BRL ) (mCM) in flat-bottomed 96-well tissue culture plates and incubated at 37°C, 5% CO2 for 3.5 d. Allogeneic T cells were prepared from C57BL/6 mice by passing RBC-depleted splenocytes through an immunoaffinity column (R & D Systems, Minneapolis, MN).

    Techniques: Derivative Assay, In Vitro, Isolation, Irradiation