immulon 4hbx 96 well plates  (Thermo Fisher)


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    Structured Review

    Thermo Fisher immulon 4hbx 96 well plates
    Immulon 4hbx 96 Well Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immulon 4hbx 96 well plates/product/Thermo Fisher
    Average 93 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    immulon 4hbx 96 well plates - by Bioz Stars, 2020-03
    93/100 stars

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    Related Articles

    Binding Assay:

    Article Title: Characterization of the Effects of Aryl-Azido Compounds and UVA Irradiation on the Viral Proteins and Infectivity of Human Immunodeficiency Virus Type 1
    Article Snippet: Neutralizing antibodies against gp120 (b12 and 2g12) or gp41(4e10) were plated on Immulon 4HBX 96-well plates (Thermo Scientific) according to previously published procedures. ( ) In brief, 100 microliters of 10 ug/mL (b12 or 2g12) or 20 ug/mL (4e10) of each antibody in carbonate buffer was added per well. .. Wells to assess background binding were also prepared using only carbonate buffer with no antibody added.

    Blocking Assay:

    Article Title: Vaccination of Rabbits with an Alkylated Toxoid Rapidly Elicits Potent Neutralizing Antibodies against Botulinum Neurotoxin Serotype B ▿
    Article Snippet: Purified active BoNT/B (Metabiologics, Inc.) was diluted to 2 μg/ml in coating buffer (PBS) and added to Immulon 4HBX 96-well plates (ThermoElectron, Rockford, IL) at 100 μl/well. .. The wells were then blocked with 100 μl blocking buffer (PBST plus 1% bovine serum albumin) for 1 h. The blocking buffer was removed, and the plates were again washed four times with PBST at 200 μl/well.

    Purification:

    Article Title: Phase I Study of a Poxviral TRICOM-Based Vaccine Directed Against the Transcription Factor Brachyury
    Article Snippet: .. Immulon 4HBX 96-well plates (Thermo Scientific) were coated with 1 μg/mL of a purified, recombinant brachyury protein or 5% BSA in PBS for 4 hours at room temperature. ..

    Article Title: Vaccination of Rabbits with an Alkylated Toxoid Rapidly Elicits Potent Neutralizing Antibodies against Botulinum Neurotoxin Serotype B ▿
    Article Snippet: .. Purified active BoNT/B (Metabiologics, Inc.) was diluted to 2 μg/ml in coating buffer (PBS) and added to Immulon 4HBX 96-well plates (ThermoElectron, Rockford, IL) at 100 μl/well. ..

    Article Title: An Immunogenic and Protective Alphavirus Replicon Particle-Based Dengue Vaccine Overcomes Maternal Antibody Interference in Weanling Mice ▿
    Article Snippet: The DENV antigen used to coat the plates consisted of DENV2 NGC purified by iodixanol density gradient centrifugation as described above. .. Immulon 4HBX 96-well plates (Thermo Electron Corp.) were coated with DENV2 antigen at 0.5 μg/ml in carbonate buffer (pH 9) and incubated overnight at 4°C.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Primary tumors induce neutrophil extracellular traps with targetable metastasis-promoting effects
    Article Snippet: Paragraph title: NETs ELISA. ... Immulon 4HBX 96-well plates (Thermo Fisher Scientific) were coated with mouse or human MPO antibody (clone 266-6K1, 1:20, Hycult Biotechnology) overnight at 4°C.

    Article Title: Vaccination of Rabbits with an Alkylated Toxoid Rapidly Elicits Potent Neutralizing Antibodies against Botulinum Neurotoxin Serotype B ▿
    Article Snippet: Paragraph title: Enzyme-linked immunosorbent assay (ELISA). ... Purified active BoNT/B (Metabiologics, Inc.) was diluted to 2 μg/ml in coating buffer (PBS) and added to Immulon 4HBX 96-well plates (ThermoElectron, Rockford, IL) at 100 μl/well.

    Article Title: Immune Responses and Hypercoagulation in ERT for Pompe Disease Are Mutation and rhGAA Dose Dependent
    Article Snippet: Paragraph title: ELISA ... Immulon 4HBX 96-well plates (Thermo-Scientific) were coated with rhGAA protein and incubated overnight at 4ο C. The following standards- IgG1κ (4000 ng/mL–62.5 ng/mL), IgG2a and IgG2b (1000 ng/mL–15 ng/mL), IgM (400 ng/mL – 6.25 ng/mL) were coated overnight at 4ο C at 2-fold dilutions.

    Article Title: Sustained Correction of Motoneuron Histopathology Following Intramuscular Delivery of AAV in Pompe Mice
    Article Snippet: Enzyme-linked immunosorbent assay for IgG1 and IgG2 were developed and optimized. .. Immulon 4HBX 96-well plates (Thermo Fisher Scientific, Waltham, MA) were coated with rhGAA protein and incubated overnight at 4 °C.

    Article Title: Copackaged AAV9 Vectors Promote Simultaneous Immune Tolerance and Phenotypic Correction of Pompe Disease
    Article Snippet: Paragraph title: Enzyme-Linked Immunosorbent Assay ... Immulon 4HBX 96-well plates (Thermo: 3855) were coated with rhGAA at 1 μg/ml or 5 × 1013 vlp/ml AAV8 or AAV9 overnight at 4°C.

    Article Title: Rapid, automated, parallel quantitative immunoassays using highly integrated microfluidics and AlphaLISA
    Article Snippet: Paragraph title: ELISA ... Briefly, Immulon 4HBX 96-well plates (Thermo Scientific, Waltham, MA) were coated with 2 μg mL−1 capture antibody in the DPBS buffer (137.0 mM NaCl, 8.0 mM Na2 HPO4 , 1.5 mM KH2 PO4 , 2.7 mM KCl, 0.1% ProClin™, pH = 7.4) over night at 4 °C.

    Article Title: An Immunogenic and Protective Alphavirus Replicon Particle-Based Dengue Vaccine Overcomes Maternal Antibody Interference in Weanling Mice ▿
    Article Snippet: Paragraph title: DENV-specific ELISA for IgG and IgG subclasses. ... Immulon 4HBX 96-well plates (Thermo Electron Corp.) were coated with DENV2 antigen at 0.5 μg/ml in carbonate buffer (pH 9) and incubated overnight at 4°C.

    Incubation:

    Article Title: Phase I Study of a Poxviral TRICOM-Based Vaccine Directed Against the Transcription Factor Brachyury
    Article Snippet: Immulon 4HBX 96-well plates (Thermo Scientific) were coated with 1 μg/mL of a purified, recombinant brachyury protein or 5% BSA in PBS for 4 hours at room temperature. .. Following overnight incubation at 4°C, plates were washed four times with PBST buffer (PBS + 0.05 % Tween-20).

    Article Title: Characterization of Zika virus binding and enhancement potential of a large panel of flavivirus murine monoclonal antibodies
    Article Snippet: Immulon 4HBX 96-well plates (ThermoScientific, Rochester, NY) were coated overnight at 4C with concentrated PRVABC59 diluted in PBS, or PBS alone. .. Plates were blocked with 3% BSA in PBS for 2h at room temperature, then washed 5x in deionized water. mAbs were serially diluted in 1% BSA in PBS, added to the plates, and incubated for 2h at room temperature.

    Article Title: Vaccination of Rabbits with an Alkylated Toxoid Rapidly Elicits Potent Neutralizing Antibodies against Botulinum Neurotoxin Serotype B ▿
    Article Snippet: Purified active BoNT/B (Metabiologics, Inc.) was diluted to 2 μg/ml in coating buffer (PBS) and added to Immulon 4HBX 96-well plates (ThermoElectron, Rockford, IL) at 100 μl/well. .. Rabbit serum was serially diluted (2-fold dilutions) in blocking buffer, added at 100 μl/well, and incubated for 1 h. Unbound antibody was removed by four washes with 200 μl/well PBST, and anti-rabbit IgG-horseradish peroxidase-mouse monoclonal antibody (Sigma) diluted 1:10,000 in blocking buffer was added at 100 μl/well.

    Article Title: Immune Responses and Hypercoagulation in ERT for Pompe Disease Are Mutation and rhGAA Dose Dependent
    Article Snippet: .. Immulon 4HBX 96-well plates (Thermo-Scientific) were coated with rhGAA protein and incubated overnight at 4ο C. The following standards- IgG1κ (4000 ng/mL–62.5 ng/mL), IgG2a and IgG2b (1000 ng/mL–15 ng/mL), IgM (400 ng/mL – 6.25 ng/mL) were coated overnight at 4ο C at 2-fold dilutions. .. Experimental mouse plasma at a 1:50 dilution was used for IgG1, IgG2a and IgG2b ELISA while 1:20 dilution was used for IgM ELISA.

    Article Title: Sustained Correction of Motoneuron Histopathology Following Intramuscular Delivery of AAV in Pompe Mice
    Article Snippet: .. Immulon 4HBX 96-well plates (Thermo Fisher Scientific, Waltham, MA) were coated with rhGAA protein and incubated overnight at 4 °C. ..

    Article Title: Copackaged AAV9 Vectors Promote Simultaneous Immune Tolerance and Phenotypic Correction of Pompe Disease
    Article Snippet: Immulon 4HBX 96-well plates (Thermo: 3855) were coated with rhGAA at 1 μg/ml or 5 × 1013 vlp/ml AAV8 or AAV9 overnight at 4°C. .. Samples were diluted 1:50 and incubated for 2 hr at 37°C and washed with PBS-T (0.05% Tween-20).

    Article Title: Rapid, automated, parallel quantitative immunoassays using highly integrated microfluidics and AlphaLISA
    Article Snippet: Briefly, Immulon 4HBX 96-well plates (Thermo Scientific, Waltham, MA) were coated with 2 μg mL−1 capture antibody in the DPBS buffer (137.0 mM NaCl, 8.0 mM Na2 HPO4 , 1.5 mM KH2 PO4 , 2.7 mM KCl, 0.1% ProClin™, pH = 7.4) over night at 4 °C. .. Microtiter plates were then loaded with recombinant human TNF-α samples spiked in the DPBS buffer and incubated at room temperature for 2 hr with 0.32 μg mL−1 detection antibody.

    Article Title: An Immunogenic and Protective Alphavirus Replicon Particle-Based Dengue Vaccine Overcomes Maternal Antibody Interference in Weanling Mice ▿
    Article Snippet: .. Immulon 4HBX 96-well plates (Thermo Electron Corp.) were coated with DENV2 antigen at 0.5 μg/ml in carbonate buffer (pH 9) and incubated overnight at 4°C. .. After excess antigen was removed, the plates were blocked using 0.05% Tween 20 in PBS with 10% Sigmablock (Sigma) for 3 h at 4°C.

    Article Title: Characterization of the Effects of Aryl-Azido Compounds and UVA Irradiation on the Viral Proteins and Infectivity of Human Immunodeficiency Virus Type 1
    Article Snippet: Neutralizing antibodies against gp120 (b12 and 2g12) or gp41(4e10) were plated on Immulon 4HBX 96-well plates (Thermo Scientific) according to previously published procedures. ( ) In brief, 100 microliters of 10 ug/mL (b12 or 2g12) or 20 ug/mL (4e10) of each antibody in carbonate buffer was added per well. .. After incubation at 4°C overnight, the wells were washed with PBS and blocked with 3% BSA for 1 hour.

    Activity Assay:

    Article Title: Sustained Correction of Motoneuron Histopathology Following Intramuscular Delivery of AAV in Pompe Mice
    Article Snippet: Homogenates were centrifuged at 14,000 rpm for 10 minutes at 4 °C and the resulting supernatant was assayed for GAA activity by measuring cleavage of 4-methylumbelliferyl-α-D-glucopyranoside after incubation for 1 hour at 37 °C. .. Immulon 4HBX 96-well plates (Thermo Fisher Scientific, Waltham, MA) were coated with rhGAA protein and incubated overnight at 4 °C.

    Spectrophotometry:

    Article Title: Copackaged AAV9 Vectors Promote Simultaneous Immune Tolerance and Phenotypic Correction of Pompe Disease
    Article Snippet: Immulon 4HBX 96-well plates (Thermo: 3855) were coated with rhGAA at 1 μg/ml or 5 × 1013 vlp/ml AAV8 or AAV9 overnight at 4°C. .. Plates were developed with Sigmafast OPD tablets (Sigma: P9187) and read using a μQuant microplate spectrophotometer (BioTek Instruments).

    Protein Concentration:

    Article Title: Sustained Correction of Motoneuron Histopathology Following Intramuscular Delivery of AAV in Pompe Mice
    Article Snippet: Protein concentration was measured using the Bio-Rad DC protein assay kit per manufacturer's instructions. .. Immulon 4HBX 96-well plates (Thermo Fisher Scientific, Waltham, MA) were coated with rhGAA protein and incubated overnight at 4 °C.

    Recombinant:

    Article Title: Phase I Study of a Poxviral TRICOM-Based Vaccine Directed Against the Transcription Factor Brachyury
    Article Snippet: .. Immulon 4HBX 96-well plates (Thermo Scientific) were coated with 1 μg/mL of a purified, recombinant brachyury protein or 5% BSA in PBS for 4 hours at room temperature. ..

    Article Title: Rapid, automated, parallel quantitative immunoassays using highly integrated microfluidics and AlphaLISA
    Article Snippet: Briefly, Immulon 4HBX 96-well plates (Thermo Scientific, Waltham, MA) were coated with 2 μg mL−1 capture antibody in the DPBS buffer (137.0 mM NaCl, 8.0 mM Na2 HPO4 , 1.5 mM KH2 PO4 , 2.7 mM KCl, 0.1% ProClin™, pH = 7.4) over night at 4 °C. .. Microtiter plates were then loaded with recombinant human TNF-α samples spiked in the DPBS buffer and incubated at room temperature for 2 hr with 0.32 μg mL−1 detection antibody.

    Gradient Centrifugation:

    Article Title: An Immunogenic and Protective Alphavirus Replicon Particle-Based Dengue Vaccine Overcomes Maternal Antibody Interference in Weanling Mice ▿
    Article Snippet: The DENV antigen used to coat the plates consisted of DENV2 NGC purified by iodixanol density gradient centrifugation as described above. .. Immulon 4HBX 96-well plates (Thermo Electron Corp.) were coated with DENV2 antigen at 0.5 μg/ml in carbonate buffer (pH 9) and incubated overnight at 4°C.

    DC Protein Assay:

    Article Title: Sustained Correction of Motoneuron Histopathology Following Intramuscular Delivery of AAV in Pompe Mice
    Article Snippet: Protein concentration was measured using the Bio-Rad DC protein assay kit per manufacturer's instructions. .. Immulon 4HBX 96-well plates (Thermo Fisher Scientific, Waltham, MA) were coated with rhGAA protein and incubated overnight at 4 °C.

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    Thermo Fisher elisa plates
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