immpact amec red substrate  (Vector Laboratories)


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    Name:
    ImmPACT AMEC Red Peroxidase HRP Substrate
    Description:
    ImmPACT AMEC Red Peroxidase Substrate is 5 10 times greater sensitivity than conventional AEC substratesOptimized for IHC ICC ISH and blotsAqueous mountingSuitable for single and multiple labelingWorking solutions stable for up to 14 days at 4 °COne year expiry dateSupplied as 120 ml of diluent and concentrated stock solutions in dropper bottles ImmPACT AMEC Red HRP Substrate utilizes Vector Laboratories ImmPACT technology to generate a crisper brighter red reaction product that is 5 10 times more sensitive than conventional AEC substrates and can be used as a single label or as a second color for multiple antigen labeling ImmPACT AMEC Red chromogen can be used for both manual and automated staining methods Stained slides must be aqueously mounted because the reaction product is soluble in organic solvents Precipitated ImmPACT AMEC Red is stable for at least 2 years when mounted in VectaMount AQ H 5501 ImmPACT AMEC Red consists of 120 ml of diluent and concentrated stock solutions of ImmPACT reagents in convenient dropper bottles This substrate formulation can be used on nylon or PVDF membranes but is not recommended for use on nitrocellulose membranes Working solutions are stable for up to 14 days at 4° C
    Catalog Number:
    SK-4285
    Price:
    None
    Category:
    Protein chromogenic detection reagents or kits or substrates
    Size:
    120 ml
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    Structured Review

    Vector Laboratories immpact amec red substrate
    ImmPACT AMEC Red Peroxidase HRP Substrate
    ImmPACT AMEC Red Peroxidase Substrate is 5 10 times greater sensitivity than conventional AEC substratesOptimized for IHC ICC ISH and blotsAqueous mountingSuitable for single and multiple labelingWorking solutions stable for up to 14 days at 4 °COne year expiry dateSupplied as 120 ml of diluent and concentrated stock solutions in dropper bottles ImmPACT AMEC Red HRP Substrate utilizes Vector Laboratories ImmPACT technology to generate a crisper brighter red reaction product that is 5 10 times more sensitive than conventional AEC substrates and can be used as a single label or as a second color for multiple antigen labeling ImmPACT AMEC Red chromogen can be used for both manual and automated staining methods Stained slides must be aqueously mounted because the reaction product is soluble in organic solvents Precipitated ImmPACT AMEC Red is stable for at least 2 years when mounted in VectaMount AQ H 5501 ImmPACT AMEC Red consists of 120 ml of diluent and concentrated stock solutions of ImmPACT reagents in convenient dropper bottles This substrate formulation can be used on nylon or PVDF membranes but is not recommended for use on nitrocellulose membranes Working solutions are stable for up to 14 days at 4° C
    https://www.bioz.com/result/immpact amec red substrate/product/Vector Laboratories
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immpact amec red substrate - by Bioz Stars, 2021-04
    94/100 stars

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    Related Articles

    Avidin-Biotin Assay:

    Article Title: Inhibition of macrophage proliferation dominates plaque regression in response to cholesterol lowering
    Article Snippet: Adjacent slides of the same plaques were stained for Oil-red O (Sigma-Aldrich, St. Louis, MO, USA). .. Secondary antibodies included rabbit–anti rat biotin conjugated (BA-4001) followed by ImmPACT AMEC Red Substrate (Vector Laboratories, Burlingame, CA, USA), rabbit–anti rat TRITC (PA1-28570, Thermofisher Scientific, Waltham, MA, USA), rabbit–anti rat AF647 (ab169349, Abcam, Cambridge, UK), goat–anti-rabbit (BA-1000) followed by fluorescein avidin DCS (A-2011, Vector Laboratories, Burlingame, CA, USA), donkey-anti-sheep TRITC (ab6897, Abcam, Cambridge, UK), alpaca-anti-rabbit AF488 (ChromoTek, Planegg-Martinsried, Germany), and DAPI Mounting Medium (Carl Roth, Karlsruhe, Germany) according to the manufacturers’ instructions. .. Images were recorded with the Axioplan 2 and Apotome 2 imaging light-/fluorescence microscope with an AxioCam camera (Carl Zeiss MicroImaging GmbH, Göttingen, Germany) and a confocal Leica TCS SP8 X microscope (Leica Microsystems, Wetzlar, Germany).

    Amplification:

    Article Title: Macrophage ATP citrate lyase deficiency stabilizes atherosclerotic plaques
    Article Snippet: MOMA-2, Ly6G, and Acly were captured using biotin-labeled rabbit anti-rat antibody (Vector Laboratories) as secondary antibody. .. Hereafter, the signal was amplified using the ABC kit (Vector Laboratories) and subsequently visualized using the ImmPACT AMEC Red substrate kit (Vector Laboratories). .. Combined MOMA-2 and TGF-β staining was visualized using Alexa-555-labeled goat anti-rat antibody (Molecular probes, 1:400) for MOMA-2 and Alexa647-labeled goat anti-rabbit antibody (Molecular probes, 1:400) for TGF-β.

    Incubation:

    Article Title: Transcriptomic signature of gut microbiome-contacting cells in colon of spontaneously hypertensive rats
    Article Snippet: Briefly, sections were washed with PBS with 0.3% Triton X-100, followed by incubation in peroxidase block solution (BLOXALL, Vector Laboratories) for 10 min. After being blocked for 1 h in normal horse serum, samples were incubated overnight at 4°C with primary antibody. .. The next day, sections were washed, incubated for 1 h at room temperature in biotinylated horse anti-rabbit/mouse secondary antibody, followed by R.T.U VECTASTAIN Elite ABC Reagent, rinsed, and then exposed to 3-3′diaminobenzidine (DAB) as the chromogenic peroxidase substrate. .. As control of immunohistochemistry detection, we evaluated the absence of primary antibody or omission of secondary antibody incubation and no immunostaining was detected under these conditions.

    Article Title: MAP kinase phosphatase 2 deficient mice develop attenuated experimental autoimmune encephalomyelitis through regulating dendritic cells and T cells
    Article Snippet: .. Frozen spinal cord tissue sections were then stained with standard haematoxylin and eosin (H & E) staining or with anti-CD45, anti-CD4, anti-CD8, anti-CD11b and anti-CD11c antibodies (all purchased from eBioscience) followed by incubation with an appropriate biotin-conjugated secondary antibody (eBioscience, UK), horseradish peroxidise and ImmPACT AMEC red peroxidase substrate (Vector Lab, UK) for detection. .. Isotypes with matching IgG were used as negative controls for all antibody staining.

    Plasmid Preparation:

    Article Title: Inhibition of PFKFB3 Hampers the Progression of Atherosclerosis and Promotes Plaque Stability
    Article Snippet: Secondary goat anti-mouse biotinylated antibody (Dako, E0433) and rabbit anti-rat biotinylated antibody (Vector, BA-4001) were used at 1:300 for 30 min. .. Slides were covered in 1:200 ABC-HRP kit (Vector laboratories) for 30 min. Epitopes were visualized with ImmPACT AMEC red (Vector, SK-4285). .. Analyses of the slides was performed on a Leica DM3000 microscope with a DFC295 camera and further analysis was done with Adobe Photoshop CS6, Image J, and Las 4.1 software (Leica).

    Staining:

    Article Title: MAP kinase phosphatase 2 deficient mice develop attenuated experimental autoimmune encephalomyelitis through regulating dendritic cells and T cells
    Article Snippet: .. Frozen spinal cord tissue sections were then stained with standard haematoxylin and eosin (H & E) staining or with anti-CD45, anti-CD4, anti-CD8, anti-CD11b and anti-CD11c antibodies (all purchased from eBioscience) followed by incubation with an appropriate biotin-conjugated secondary antibody (eBioscience, UK), horseradish peroxidise and ImmPACT AMEC red peroxidase substrate (Vector Lab, UK) for detection. .. Isotypes with matching IgG were used as negative controls for all antibody staining.

    Activity Assay:

    Article Title: Reducing GBA2 Activity Ameliorates Neuropathology in Niemann-Pick Type C Mice
    Article Snippet: Subsequently, sections were incubated with normal goat serum, rabbit anti-GBA2, and HRP-conjugated goat anti-rabbit IgG. .. HRP activity was detected using ImmPACT AMEC Red substrate (SK-4285; Vector Laboratories). .. Sections were counterstained with methyl green and mounted with VectaMount (Vector Laboratories).

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  • 94
    Vector Laboratories immpact amec red substrate
    Immpact Amec Red Substrate, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immpact amec red substrate/product/Vector Laboratories
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immpact amec red substrate - by Bioz Stars, 2021-04
    94/100 stars
      Buy from Supplier

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