immobilon western detection kit  (Millipore)


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  • 90

    Structured Review

    Millipore immobilon western detection kit
    Immobilon Western Detection Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immobilon western detection kit/product/Millipore
    Average 90 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    immobilon western detection kit - by Bioz Stars, 2020-07
    90/100 stars

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    Related Articles

    Western Blot:

    Article Title: Fecal transplant modifies urine chemistry risk factors for urinary stone disease. Fecal transplant modifies urine chemistry risk factors for urinary stone disease
    Article Snippet: .. Protein bands were detected on the Azure c600 Bioanalytical Imaging System (Azure Biosystem, Dublin, CA) using the Immobilon Western detection kit (Millipore, MA). .. Densitometric analyses were performed using ImageJ (NIH) software.

    Article Title: A high-throughput FRET-based assay for determination of Atg4 activity
    Article Snippet: .. After the application of the appropriate antibodies, signals were developed using Immobilon Western detection kit (Millipore, WBKLS0500) according to the manufacturer’s instructions. .. Analysis of the Atg4-mediated cleavage of FRET-substrates by SDS-PAGE Purified FRET-LC3B, FRET-LC3BG120A and FRET-GATE-16 (5 µg) were mixed with 0.25 µg of purified Atg4A, Atg4B or Atg4BC74S or 25 µg of HEK-293A cell lysate in Buffer B (150 mM NaCl, 1 mM EDTA, 50 mM TRIS-HCl, pH 7.5).

    Article Title: The adipokine lipocalin-2 in the context of the osteoarthritic osteochondral junction
    Article Snippet: .. The antibody binding was visualized by enhanced chemiluminescence with the corresponding peroxidase-conjugated secondary antibody and Immobilon Western Detection kit (EMD Millipore, Billerica, MA, USA). .. To confirm equal loading of each sample, the expression of LCN2 was normalized to actin expression.

    Article Title: Kinetics Comparisons of Mammalian Atg4 Homologues Indicate Selective Preferences toward Diverse Atg8 Substrates *
    Article Snippet: .. After the application of the appropriate antibodies, signals were developed using an Immobilon Western detection kit (Millipore, Billerica, MA) according to the manufacturer's instructions. .. To determine and to compare the enzymatic characteristics of the Atg4 homologues, we prepared recombinant proteins using a bacterial expression system.

    Article Title: SERPINE2 Inhibits IL-1α-Induced MMP-13 Expression in Human Chondrocytes: Involvement of ERK/NF-κB/AP-1 Pathways
    Article Snippet: .. Immunoblots have been visualized with Immobilon Western Detection kit (Millipore, MA) using horseradish peroxidase-labeled secondary antibody. .. To confirm equal loading in each sample, the membranes were stripped in stripping buffer (100 mM β-mercaptoethanol, 2% SDS, 62.5 mM Tris-HCl pH 6.7) and re-blotted with anti-β-actin (Sigma, MO, USA) or anti-lamin-β1 antibody (GeneTex, CA, USA).

    Article Title: Connexin43 and Pannexin1 Channels in Osteoblasts: Who is the "hemichannel"?
    Article Snippet: .. Collagenase Type II was purchased from Worthington Biochemical Corporation (Lakewood, NJ, USA), protease inhibitor cocktail was purchased from Roche (Mannheim, Germany), nitrocellulose membranes were purchased from Whatman GmbH (Dassel, Germany) and Immobilon Western detection kit were purchased from Millipore (Billerica, MA, USA). ..

    Article Title: Role of ER Stress in Ventricular Contractile Dysfunction in Type 2 Diabetes
    Article Snippet: .. Immunoblotted proteins were visualized by using an Immobilon Western detection kit (Millipore, Billerica, MA) and quantified by a lumino-image analyzer, LAS-2000mini (Fujifilm, Tokyo, Japan). .. Immunoprecipitation Study An equal amount of protein (500 µg) from the sarcoplasmic reticulum fraction used in immunoblotting was solubilized in a CelLytic™ MT Mammalian Tissue Lysis/Extraction Reagent (Sigma Aldrich) including protease inhibitor cocktail (Nakalai Tesque, Kyoto, Japan) and a proteasome inhibitor, MG132, (10 µM) for 60 min at 4°C.

    Article Title: Role of pannexin 1 channels in load-induced skeletal response
    Article Snippet: .. The protein bands were detected with the In Vivo FX PRO imaging system (Carestream, NY) using the Immobilon western detection kit (Millipore, MA), as previously described. .. Densitometric analyses were performed using ImageJ (NIH) software.

    In Vivo:

    Article Title: Role of pannexin 1 channels in load-induced skeletal response
    Article Snippet: .. The protein bands were detected with the In Vivo FX PRO imaging system (Carestream, NY) using the Immobilon western detection kit (Millipore, MA), as previously described. .. Densitometric analyses were performed using ImageJ (NIH) software.

    Binding Assay:

    Article Title: The adipokine lipocalin-2 in the context of the osteoarthritic osteochondral junction
    Article Snippet: .. The antibody binding was visualized by enhanced chemiluminescence with the corresponding peroxidase-conjugated secondary antibody and Immobilon Western Detection kit (EMD Millipore, Billerica, MA, USA). .. To confirm equal loading of each sample, the expression of LCN2 was normalized to actin expression.

    Imaging:

    Article Title: Fecal transplant modifies urine chemistry risk factors for urinary stone disease. Fecal transplant modifies urine chemistry risk factors for urinary stone disease
    Article Snippet: .. Protein bands were detected on the Azure c600 Bioanalytical Imaging System (Azure Biosystem, Dublin, CA) using the Immobilon Western detection kit (Millipore, MA). .. Densitometric analyses were performed using ImageJ (NIH) software.

    Article Title: Role of pannexin 1 channels in load-induced skeletal response
    Article Snippet: .. The protein bands were detected with the In Vivo FX PRO imaging system (Carestream, NY) using the Immobilon western detection kit (Millipore, MA), as previously described. .. Densitometric analyses were performed using ImageJ (NIH) software.

    Protease Inhibitor:

    Article Title: Connexin43 and Pannexin1 Channels in Osteoblasts: Who is the "hemichannel"?
    Article Snippet: .. Collagenase Type II was purchased from Worthington Biochemical Corporation (Lakewood, NJ, USA), protease inhibitor cocktail was purchased from Roche (Mannheim, Germany), nitrocellulose membranes were purchased from Whatman GmbH (Dassel, Germany) and Immobilon Western detection kit were purchased from Millipore (Billerica, MA, USA). ..

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    Millipore immobilon western chemiluminescent hrp substrate detection kit
    Immobilon Western Chemiluminescent Hrp Substrate Detection Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immobilon western chemiluminescent hrp substrate detection kit/product/Millipore
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    immobilon western chemiluminescent hrp substrate detection kit - by Bioz Stars, 2020-07
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    Millipore immobilon western blotting detection kit
    Immobilon Western Blotting Detection Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immobilon western blotting detection kit/product/Millipore
    Average 89 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    immobilon western blotting detection kit - by Bioz Stars, 2020-07
    89/100 stars
      Buy from Supplier

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    Millipore chemiluminescence detection kit
    Chemiluminescence Detection Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 100 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chemiluminescence detection kit/product/Millipore
    Average 99 stars, based on 100 article reviews
    Price from $9.99 to $1999.99
    chemiluminescence detection kit - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

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