immobilon western blotting detection kit  (Millipore)


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    Structured Review

    Millipore immobilon western blotting detection kit
    Immobilon Western Blotting Detection Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immobilon western blotting detection kit/product/Millipore
    Average 99 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    immobilon western blotting detection kit - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: Adenovirus E3-6.7K Maintains Calcium Homeostasis and Prevents Apoptosis and Arachidonic Acid Release
    Article Snippet: .. Equivalent amounts of protein from each sample were resolved on a 10% glycine-SDS-polyacrylamide gel electrophoresis (PAGE) Laemmli gel system, blotted with the Towbin vertical-transfer wet system onto a 0.45-μm-pore-size Immobilon-P polyvinylidene difluoride (PVDF) membrane (Millipore), and incubated with anti-PARP mouse monoclonal antibody (1:5,000 dilution; Pharmingen). .. After four washes in TBS containing 0.1% Tween 20 detergent (TBS-T), the blot was incubated with horseradish peroxidase-conjugated goat anti-mouse antiserum (1:50,000 dilution) and visualized by chemiluminescence with a SuperSignal West Pico kit (Pierce Chemical).

    Electrophoresis:

    Article Title: The ClpXP ATP-Dependent Protease Regulates Flagellum Synthesis in Salmonella enterica Serovar Typhimurium
    Article Snippet: .. For immunoblot analysis, proteins separated on the SDS-12% polyacrylamide gels were transferred after electrophoresis onto Immobilon-P (Millipore) as previously reported ( ). .. Proteins were reacted with either rabbit anti- S. enterica serovar Typhimurium FliC (1:25,000) or FliA (1:12,500) antiserum, followed by treatment with alkaline phosphatase-conjugated anti-rabbit immunoglobulin G. The enzymatic reactions were performed in the presence of 300 μg of nitroblue tetrazolium (Dojindo, Kumamoto, Japan)/ml and 150 μg of bromochloroindolyl phosphate (aMReSCO, Solon, Ohio)/ml.

    Immunoprecipitation:

    Article Title: Dimerization of the Polymeric Immunoglobulin Receptor Controls Its Transcytotic Trafficking
    Article Snippet: .. After the nuclei had been spun out, lysates were cleared 2× with empty protein A Sepharose (Pharmacia, Uppsala, Sweden), immunoprecipitated with anti-ζ monoclonal 6810.2 (a kind gift from Art Weiss) bound to protein A, resolved by 15% SDS-PAGE, and transferred to Immobilon P (Millipore, Bedford, MA) for Western blot with mouse monoclonal 4G10 (anti-phosphotyrosine, Upstate Biotechnology, Lake Placid, NY). ..

    Incubation:

    Article Title: Adenovirus E3-6.7K Maintains Calcium Homeostasis and Prevents Apoptosis and Arachidonic Acid Release
    Article Snippet: .. Equivalent amounts of protein from each sample were resolved on a 10% glycine-SDS-polyacrylamide gel electrophoresis (PAGE) Laemmli gel system, blotted with the Towbin vertical-transfer wet system onto a 0.45-μm-pore-size Immobilon-P polyvinylidene difluoride (PVDF) membrane (Millipore), and incubated with anti-PARP mouse monoclonal antibody (1:5,000 dilution; Pharmingen). .. After four washes in TBS containing 0.1% Tween 20 detergent (TBS-T), the blot was incubated with horseradish peroxidase-conjugated goat anti-mouse antiserum (1:50,000 dilution) and visualized by chemiluminescence with a SuperSignal West Pico kit (Pierce Chemical).

    Activity Assay:

    Article Title: Characterization of the autophosphorylation property of HflX, a ribosome‐binding GTPase from Escherichia coli
    Article Snippet: .. The Ni+2 ‐nitrilotriacetic acid resin (QIAGEN GmbH, Hilden, Germany), dNTP mix (Fermentas Life Sciences, Waltham, MA, USA), Immobilon‐P (PVDF, polyvinylidene fluoride) membrane (Millipore, Billerica, MA, USA) and [γ32 ‐P] GTP (specific activity 4500 Ci·mmol−1 ) (BRIT, Mumbai, India) were purchased from the indicated sources. .. Cellulose thin layer (100 μm) chromatography plates (polyester sigmacell type 100 cellulose) of dimensions 20 cm × 20 cm, were purchased from Sigma.

    Polyacrylamide Gel Electrophoresis:

    Article Title: Adenovirus E3-6.7K Maintains Calcium Homeostasis and Prevents Apoptosis and Arachidonic Acid Release
    Article Snippet: .. Equivalent amounts of protein from each sample were resolved on a 10% glycine-SDS-polyacrylamide gel electrophoresis (PAGE) Laemmli gel system, blotted with the Towbin vertical-transfer wet system onto a 0.45-μm-pore-size Immobilon-P polyvinylidene difluoride (PVDF) membrane (Millipore), and incubated with anti-PARP mouse monoclonal antibody (1:5,000 dilution; Pharmingen). .. After four washes in TBS containing 0.1% Tween 20 detergent (TBS-T), the blot was incubated with horseradish peroxidase-conjugated goat anti-mouse antiserum (1:50,000 dilution) and visualized by chemiluminescence with a SuperSignal West Pico kit (Pierce Chemical).

    Western Blot:

    Article Title: Dimerization of the Polymeric Immunoglobulin Receptor Controls Its Transcytotic Trafficking
    Article Snippet: .. After the nuclei had been spun out, lysates were cleared 2× with empty protein A Sepharose (Pharmacia, Uppsala, Sweden), immunoprecipitated with anti-ζ monoclonal 6810.2 (a kind gift from Art Weiss) bound to protein A, resolved by 15% SDS-PAGE, and transferred to Immobilon P (Millipore, Bedford, MA) for Western blot with mouse monoclonal 4G10 (anti-phosphotyrosine, Upstate Biotechnology, Lake Placid, NY). ..

    SDS Page:

    Article Title: Dimerization of the Polymeric Immunoglobulin Receptor Controls Its Transcytotic Trafficking
    Article Snippet: .. After the nuclei had been spun out, lysates were cleared 2× with empty protein A Sepharose (Pharmacia, Uppsala, Sweden), immunoprecipitated with anti-ζ monoclonal 6810.2 (a kind gift from Art Weiss) bound to protein A, resolved by 15% SDS-PAGE, and transferred to Immobilon P (Millipore, Bedford, MA) for Western blot with mouse monoclonal 4G10 (anti-phosphotyrosine, Upstate Biotechnology, Lake Placid, NY). ..

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  • 89
    Millipore immobilon western blotting detection kit
    Immobilon Western Blotting Detection Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immobilon western blotting detection kit/product/Millipore
    Average 89 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    immobilon western blotting detection kit - by Bioz Stars, 2020-07
    89/100 stars
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