Journal: Nature Communications
Article Title: DNA computing function switching by programming base stacking interactions with minimal molecular architecture changes
doi: 10.1038/s41467-025-64406-x
Figure Lengend Snippet: a Schematic representation of the SMALL microRNA logic gate for endogenous microRNA imaging. b Schematic diagram of the SMALL microRNA gates with different stacking interactions combinations. c Confocal laser scanning microscopy (CLSM) imaging results of FAM green fluorescence in the MCF-7 cells after treatment with the AND and OR SMALL microRNA gates. The selective microRNA control groups were implemented by pretreating specific inhibitors for microRNAs 21 and 17. d FAM green fluorescence intensity statistics of the MCF-7 cells after treatment with the dual-input AND and OR gates, as shown in Figure ( c ) with 20 um scale bars (48 h, mean ± SD, n = 4 from biologically independent experiments). e , f Schematic illustration and the design details of the multi-input SMALL microRNA AND gate regulated by four stacking interactions 1, 2, 3 and 4. g CLSM imaging results of FAM green fluorescence in the HeLa cells after treatment with the four-input SMALL microRNA AND gate. h FAM green fluorescence intensity statistics of the HeLa cells after treatment with the four-input AND gate, as shown in Figure ( g ) with 20 um scale bars (48 h, mean ± SD, n = 4 from biologically independent experiments). i Flow cytometry analysis of FAM green fluorescence in the HeLa cells treated with the four-input AND gate in different microRNA control groups. The data were analyzed using GraphPad Prism 8.0 and SPSS 17.0. All error bars indicate the standard deviation (SD) of the mean. One-way ANOVA was applied for multiple comparisons, and the two-sided test was used for comparison between two groups: * P < 0.05; ** P < 0.01; and *** P < 0.001. Source data are provided as a Source Data file.
Article Snippet: The captured images were subjected to further analysis utilizing the Imaris microscopy image analysis software.
Techniques: Imaging, Confocal Laser Scanning Microscopy, Fluorescence, Control, Flow Cytometry, Standard Deviation, Comparison