Structured Review

GE Healthcare imagequant las 4000
( a . ( b ) Epitope mapping of Fab-6HD5 against the IgE H chain constant region. GST fusion molecules containing IgE Cε domains (Cε1-4, Cε1-2, Cε3-4, Cε1, Cε2) were run on 12.5% SDS-PAGE under reducing conditions, transferred to a PVDF membrane and probed with Fab-6HD5 followed by Pox goat anti-rat IgG. Immunoreactivity was detected using an <t>Imagequant</t> LAS 4000. ( c) . ( d ) Epitope mapping of Fab-6HD5 against the IgE Cε2 domain. GST fusion molecules containing IgE Cε fragments were run on 12.5% SDS-PAGE under reducing conditions, transferred to a PVDF membrane and probed with Fab-6HD5 followed by Pox goat anti-rat IgG. Immunoreactivity was detected using an Imagequant LAS 4000.
Imagequant Las 4000, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 783 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/imagequant las 4000/product/GE Healthcare
Average 94 stars, based on 783 article reviews
Price from $9.99 to $1999.99
imagequant las 4000 - by Bioz Stars, 2020-07
94/100 stars

Images

1) Product Images from "The Fab fragment of anti-IgE Cε2 domain prevents allergic reactions through interacting with IgE-FcεRIα complex on rat mast cells"

Article Title: The Fab fragment of anti-IgE Cε2 domain prevents allergic reactions through interacting with IgE-FcεRIα complex on rat mast cells

Journal: Scientific Reports

doi: 10.1038/s41598-018-32200-z

( a . ( b ) Epitope mapping of Fab-6HD5 against the IgE H chain constant region. GST fusion molecules containing IgE Cε domains (Cε1-4, Cε1-2, Cε3-4, Cε1, Cε2) were run on 12.5% SDS-PAGE under reducing conditions, transferred to a PVDF membrane and probed with Fab-6HD5 followed by Pox goat anti-rat IgG. Immunoreactivity was detected using an Imagequant LAS 4000. ( c) . ( d ) Epitope mapping of Fab-6HD5 against the IgE Cε2 domain. GST fusion molecules containing IgE Cε fragments were run on 12.5% SDS-PAGE under reducing conditions, transferred to a PVDF membrane and probed with Fab-6HD5 followed by Pox goat anti-rat IgG. Immunoreactivity was detected using an Imagequant LAS 4000.
Figure Legend Snippet: ( a . ( b ) Epitope mapping of Fab-6HD5 against the IgE H chain constant region. GST fusion molecules containing IgE Cε domains (Cε1-4, Cε1-2, Cε3-4, Cε1, Cε2) were run on 12.5% SDS-PAGE under reducing conditions, transferred to a PVDF membrane and probed with Fab-6HD5 followed by Pox goat anti-rat IgG. Immunoreactivity was detected using an Imagequant LAS 4000. ( c) . ( d ) Epitope mapping of Fab-6HD5 against the IgE Cε2 domain. GST fusion molecules containing IgE Cε fragments were run on 12.5% SDS-PAGE under reducing conditions, transferred to a PVDF membrane and probed with Fab-6HD5 followed by Pox goat anti-rat IgG. Immunoreactivity was detected using an Imagequant LAS 4000.

Techniques Used: SDS Page

2) Product Images from "The Fab fragment of anti-IgE Cε2 domain prevents allergic reactions through interacting with IgE-FcεRIα complex on rat mast cells"

Article Title: The Fab fragment of anti-IgE Cε2 domain prevents allergic reactions through interacting with IgE-FcεRIα complex on rat mast cells

Journal: Scientific Reports

doi: 10.1038/s41598-018-32200-z

( a ) GST-IgE H chain constant region fusion molecules. cDNA was prepared from the mouse IgE-producing hybridoma SPE-7. The resulting cDNA was amplified for the coding gene of the IgE H chain constant region (Cε1-4) using the PCR primer sets listed in Supplementary Table 1 . ( b ) Epitope mapping of Fab-6HD5 against the IgE H chain constant region. GST fusion molecules containing IgE Cε domains (Cε1-4, Cε1-2, Cε3-4, Cε1, Cε2) were run on 12.5% SDS-PAGE under reducing conditions, transferred to a PVDF membrane and probed with Fab-6HD5 followed by Pox goat anti-rat IgG. Immunoreactivity was detected using an Imagequant LAS 4000. ( c) GST fusion molecules containing IgE Cε2 fragments. cDNA was prepared from the mouse IgE producing hybridoma, SPE-7. The resulting cDNA was amplified for the coding gene of IgE Cε2 fragments using the PCR primer sets listed in Supplementary Table 1 . ( d ) Epitope mapping of Fab-6HD5 against the IgE Cε2 domain. GST fusion molecules containing IgE Cε fragments were run on 12.5% SDS-PAGE under reducing conditions, transferred to a PVDF membrane and probed with Fab-6HD5 followed by Pox goat anti-rat IgG. Immunoreactivity was detected using an Imagequant LAS 4000.
Figure Legend Snippet: ( a ) GST-IgE H chain constant region fusion molecules. cDNA was prepared from the mouse IgE-producing hybridoma SPE-7. The resulting cDNA was amplified for the coding gene of the IgE H chain constant region (Cε1-4) using the PCR primer sets listed in Supplementary Table 1 . ( b ) Epitope mapping of Fab-6HD5 against the IgE H chain constant region. GST fusion molecules containing IgE Cε domains (Cε1-4, Cε1-2, Cε3-4, Cε1, Cε2) were run on 12.5% SDS-PAGE under reducing conditions, transferred to a PVDF membrane and probed with Fab-6HD5 followed by Pox goat anti-rat IgG. Immunoreactivity was detected using an Imagequant LAS 4000. ( c) GST fusion molecules containing IgE Cε2 fragments. cDNA was prepared from the mouse IgE producing hybridoma, SPE-7. The resulting cDNA was amplified for the coding gene of IgE Cε2 fragments using the PCR primer sets listed in Supplementary Table 1 . ( d ) Epitope mapping of Fab-6HD5 against the IgE Cε2 domain. GST fusion molecules containing IgE Cε fragments were run on 12.5% SDS-PAGE under reducing conditions, transferred to a PVDF membrane and probed with Fab-6HD5 followed by Pox goat anti-rat IgG. Immunoreactivity was detected using an Imagequant LAS 4000.

Techniques Used: Amplification, Polymerase Chain Reaction, SDS Page

3) Product Images from "Deletion of DDB1- and CUL4- associated factor-17 (Dcaf17) gene causes spermatogenesis defects and male infertility in mice"

Article Title: Deletion of DDB1- and CUL4- associated factor-17 (Dcaf17) gene causes spermatogenesis defects and male infertility in mice

Journal: Scientific Reports

doi: 10.1038/s41598-018-27379-0

Diagrammatic representation (A) of Dcaf17 gene targeting approach in mouse by homologous recombination and genotyping ( B , C ) of different alleles of Dcaf17 in mice. ( A ) Homologous recombination strategy in mouse ES cells. The Dcaf17 targeting vector (top) was constructed to replace wild type exon 4 and introduce neomycin drug selection marker, LoxP and FRT sites. ( B ) Agarose gel image of PCR genotyping of representative Dcaf17 mutant mice. PCR amplification of wild type genotype gives 1 kbps amplicon (1, C3), heterozygous genotype for Dcaf17 mutation gives 1 kbps and 193 bps amplicons (3–5, C1) and homozygous genotype for Dcaf17 mutation gives 193 bps amplicon (2, 6 and C2). 1–6 – genomic DNA samples of different Dcaf17 genotypes; C1-C3 – Different Dcaf17 genotype controls; −Ve – no template control. ( C ) Agarose gel image of RT-PCR of different Dcaf17 genotypes. PCR products of various Dcaf17 alleles and β-actin were run on the same agarose gel and single image was taken. +/+ - Dcaf17 +/+ (WT); +/− - Dcaf17 +/− (heterozygous Dcaf17 mutant); −/− - Dcaf17 −/− (homozygous Dcaf17 mutant); −Ve – no template control; M – DNA ladder. PCR fragment size for β-actin is 190 bps; for Dcaf17 +/+ is 284 bps and for Dcaf17 −/− is 148 bps. Gel images were taken using ImageQuant LAS 4000 imaging system.
Figure Legend Snippet: Diagrammatic representation (A) of Dcaf17 gene targeting approach in mouse by homologous recombination and genotyping ( B , C ) of different alleles of Dcaf17 in mice. ( A ) Homologous recombination strategy in mouse ES cells. The Dcaf17 targeting vector (top) was constructed to replace wild type exon 4 and introduce neomycin drug selection marker, LoxP and FRT sites. ( B ) Agarose gel image of PCR genotyping of representative Dcaf17 mutant mice. PCR amplification of wild type genotype gives 1 kbps amplicon (1, C3), heterozygous genotype for Dcaf17 mutation gives 1 kbps and 193 bps amplicons (3–5, C1) and homozygous genotype for Dcaf17 mutation gives 193 bps amplicon (2, 6 and C2). 1–6 – genomic DNA samples of different Dcaf17 genotypes; C1-C3 – Different Dcaf17 genotype controls; −Ve – no template control. ( C ) Agarose gel image of RT-PCR of different Dcaf17 genotypes. PCR products of various Dcaf17 alleles and β-actin were run on the same agarose gel and single image was taken. +/+ - Dcaf17 +/+ (WT); +/− - Dcaf17 +/− (heterozygous Dcaf17 mutant); −/− - Dcaf17 −/− (homozygous Dcaf17 mutant); −Ve – no template control; M – DNA ladder. PCR fragment size for β-actin is 190 bps; for Dcaf17 +/+ is 284 bps and for Dcaf17 −/− is 148 bps. Gel images were taken using ImageQuant LAS 4000 imaging system.

Techniques Used: Homologous Recombination, Mouse Assay, Plasmid Preparation, Construct, Introduce, Selection, Marker, Agarose Gel Electrophoresis, Polymerase Chain Reaction, Mutagenesis, Amplification, Reverse Transcription Polymerase Chain Reaction, Imaging

4) Product Images from "Cyclic AMP Inhibits the Activity and Promotes the Acetylation of Acetyl-CoA Synthetase through Competitive Binding to the ATP/AMP Pocket *"

Article Title: Cyclic AMP Inhibits the Activity and Promotes the Acetylation of Acetyl-CoA Synthetase through Competitive Binding to the ATP/AMP Pocket *

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.M116.753640

cAMP promotes acetylation of Se Acs Lys 609 in vitro . A, the antibody used is specific to acetylated Se Acs Lys 609 . The same amounts of Se Acs isolated from cobB -null or pat -null S. enterica strains were separated by SDS-PAGE, transferred to the nitrocellulose membrane, then blotted with the antibody specific to the acetylated Se Acs Lys 609 in the presence or absence of unmodified peptide (KTRSGKIMRRI), or acetylated peptide (KTRSG(K ac )IMRRI), and detected using an ImageQuant LAS 4000 (GE Healthcare). Coomassie Brilliant Blue staining was used for loading controls. B, semiquantification of the acetylation level of Se Acs Lys 609 purified from CobB -null or pat -null S. enterica cells. C, cAMP activates Se Pat-dependent acetylation and enhances the activity inhibition upon Se Acs. Recombinant Se Acs purified from the pat -null S. enterica strain is treated by acetylation in the presence or absence of cAMP followed by activity measurement and Western blotting detection. The Se Acs activity in the absence of cAMP, acetyl-CoA, and Se Pat ( Lane 1 ) was treated as 100%. Coomassie Brilliant Blue-stained proteins on SDS-PAGE gels are shown below as a loading control. D, cAMP inhibits Se Cob-dependent deacetylation and weakens the activity recovery of Se Acs. Recombinant Se Acs purified from a cobB -null S. enterica strain was treated by deacetylation in the presence or absence of cAMP followed by activity measurement and Western blotting detection. The Se Acs activity ( lane 4 ) was treated as 100%. Each experiment was repeated in triplicate, and the data are presented as mean ± S.D., ***, p
Figure Legend Snippet: cAMP promotes acetylation of Se Acs Lys 609 in vitro . A, the antibody used is specific to acetylated Se Acs Lys 609 . The same amounts of Se Acs isolated from cobB -null or pat -null S. enterica strains were separated by SDS-PAGE, transferred to the nitrocellulose membrane, then blotted with the antibody specific to the acetylated Se Acs Lys 609 in the presence or absence of unmodified peptide (KTRSGKIMRRI), or acetylated peptide (KTRSG(K ac )IMRRI), and detected using an ImageQuant LAS 4000 (GE Healthcare). Coomassie Brilliant Blue staining was used for loading controls. B, semiquantification of the acetylation level of Se Acs Lys 609 purified from CobB -null or pat -null S. enterica cells. C, cAMP activates Se Pat-dependent acetylation and enhances the activity inhibition upon Se Acs. Recombinant Se Acs purified from the pat -null S. enterica strain is treated by acetylation in the presence or absence of cAMP followed by activity measurement and Western blotting detection. The Se Acs activity in the absence of cAMP, acetyl-CoA, and Se Pat ( Lane 1 ) was treated as 100%. Coomassie Brilliant Blue-stained proteins on SDS-PAGE gels are shown below as a loading control. D, cAMP inhibits Se Cob-dependent deacetylation and weakens the activity recovery of Se Acs. Recombinant Se Acs purified from a cobB -null S. enterica strain was treated by deacetylation in the presence or absence of cAMP followed by activity measurement and Western blotting detection. The Se Acs activity ( lane 4 ) was treated as 100%. Each experiment was repeated in triplicate, and the data are presented as mean ± S.D., ***, p

Techniques Used: In Vitro, Isolation, SDS Page, Staining, Purification, Activity Assay, Inhibition, Recombinant, Western Blot

Related Articles

Incubation:

Article Title: DNA methyltransferase inhibition overcomes diphthamide pathway deficiencies underlying CD123-targeted treatment resistance
Article Snippet: .. The blots were washed 3 times in TBS with 0.1% Tween before being incubated with either rabbit (Santa Cruz Biotechnology, SC-2004) or mouse (Santa Cruz Biotechnology, SC-2005) secondary HRP-conjugated antibodies before being imaged using ECL substrate (Bio-Rad, 170-5061) on an ImageQuant LAS-4000 (GE Healthcare, 28-9607-59AB). .. Lysates were prepared from cell lines in RIPA buffer (Boston BioProducts, BP-115) with protease inhibitor (Thermo Fisher Scientific, 1862209) and sonicated before quantification with BCA assay (Thermo Fisher Scientific, 23225).

Agarose Gel Electrophoresis:

Article Title: Deletion of DDB1- and CUL4- associated factor-17 (Dcaf17) gene causes spermatogenesis defects and male infertility in mice
Article Snippet: .. Agarose gel images were taken using ImageQuant LAS 4000 (GE Healthcare Life Sciences, Pittsburgh,PA, USA) gel imaging system under standard UV exposure of 1/8 seconds. .. Adobe Photoshop software (Adobe Inc., San Jose, CA, USA) and Microsoft PowerPoint software were used to assemble images into figures.

Imaging:

Article Title: Deletion of DDB1- and CUL4- associated factor-17 (Dcaf17) gene causes spermatogenesis defects and male infertility in mice
Article Snippet: .. Agarose gel images were taken using ImageQuant LAS 4000 (GE Healthcare Life Sciences, Pittsburgh,PA, USA) gel imaging system under standard UV exposure of 1/8 seconds. .. Adobe Photoshop software (Adobe Inc., San Jose, CA, USA) and Microsoft PowerPoint software were used to assemble images into figures.

SDS Page:

Article Title: Cyclic AMP Inhibits the Activity and Promotes the Acetylation of Acetyl-CoA Synthetase through Competitive Binding to the ATP/AMP Pocket *
Article Snippet: .. Proteins were separated by SDS-PAGE, transferred onto NC membrane (Millipore), subjected to immunoblotting with the homemade antibody specific to the acetylated Se Acs Lys609 , and detected by ImageQuant LAS 4000 (GE Healthcare). .. Coomassie Blue staining (SDS-PAGE) was used for loading controls.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 89
    GE Healthcare imagequanttm las 4000 mini
    Imagequanttm Las 4000 Mini, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 89/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/imagequanttm las 4000 mini/product/GE Healthcare
    Average 89 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    imagequanttm las 4000 mini - by Bioz Stars, 2020-07
    89/100 stars
      Buy from Supplier

    88
    GE Healthcare imagequanttm las 4000 mini biomolecular imager
    Imagequanttm Las 4000 Mini Biomolecular Imager, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/imagequanttm las 4000 mini biomolecular imager/product/GE Healthcare
    Average 88 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    imagequanttm las 4000 mini biomolecular imager - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    88
    GE Healthcare imagequanttm las 4000
    Imagequanttm Las 4000, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/imagequanttm las 4000/product/GE Healthcare
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    imagequanttm las 4000 - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    Image Search Results