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GE Healthcare chemiluminescence ecl ge
Chemiluminescence Ecl Ge, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 80/100, based on 9660 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/chemiluminescence ecl ge/product/GE Healthcare
Average 80 stars, based on 9660 article reviews
Price from $9.99 to $1999.99
chemiluminescence ecl ge - by Bioz Stars, 2020-04
80/100 stars

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Cytometry:

Article Title: Role of IRF4 in resistance to immunomodulatory (IMid) compounds® in Waldenström’s macroglobulinemia
Article Snippet: Staining was detected and analyzed on a CyAn ADP analyzer flow cytometer (Beckman Coulter Inc.). .. Quantification was done using ImageQuant TL software (GE Healthcare, Chicago, IL).

Enzyme-linked Immunosorbent Assay:

Article Title: Decreased Plasma Aβ in Hyperlipidemic APPSL Transgenic Mice Is Associated with BBB Dysfunction
Article Snippet: Paragraph title: Semi-quantitative ELISA ... Antibodies: anti-mouse IgG biotinylated (Eubio, BA-2000; 1:5000) + anti-streptavidin HRP conjugated (Zymed, 43-4323; 1:5000); anti-occludin (abcam, ab167161; 1:1000) + anti-rabbit IgG, HRP-linked (GE-Healthcare, NA934; 1:5000); anti-ZO1 tight junction protein (abcam, ab59720; 1:100) + anti-rabbit IgG, HRP-linked (GE-Healthcare, NA934; 1:2500); anti-Vascular Cell Adhesion Molecule 1 (VCAM-1) (Santa Cruz, sc-1504-R; 1:500) + anti-rabbit IgG, HRP-linked (GE-Healthcare, NA934; 1:5000); anti-LRP1 [alpha-2-macroglobulin receptor] (Santa Cruz, sc-57351; 1:500) + anti-mouse IgG, peroxidase linked (GE-Healthcare, NA931; 1:1000); anti-Cluster of Differentiation 31 (CD31) (abcam, ab28364; 1:250) + anti-rabbit IgG, HRP-linked (GE-Healthcare, NA934; 1:1000).

Incubation:

Article Title: Decreased Plasma Aβ in Hyperlipidemic APPSL Transgenic Mice Is Associated with BBB Dysfunction
Article Snippet: After 30 min incubation in the dark, reaction was stopped with 100 μl 0.5 M H2 SO4 and measured at 450 nm with μQuant universal microplate spectrophotometer. .. Antibodies: anti-mouse IgG biotinylated (Eubio, BA-2000; 1:5000) + anti-streptavidin HRP conjugated (Zymed, 43-4323; 1:5000); anti-occludin (abcam, ab167161; 1:1000) + anti-rabbit IgG, HRP-linked (GE-Healthcare, NA934; 1:5000); anti-ZO1 tight junction protein (abcam, ab59720; 1:100) + anti-rabbit IgG, HRP-linked (GE-Healthcare, NA934; 1:2500); anti-Vascular Cell Adhesion Molecule 1 (VCAM-1) (Santa Cruz, sc-1504-R; 1:500) + anti-rabbit IgG, HRP-linked (GE-Healthcare, NA934; 1:5000); anti-LRP1 [alpha-2-macroglobulin receptor] (Santa Cruz, sc-57351; 1:500) + anti-mouse IgG, peroxidase linked (GE-Healthcare, NA931; 1:1000); anti-Cluster of Differentiation 31 (CD31) (abcam, ab28364; 1:250) + anti-rabbit IgG, HRP-linked (GE-Healthcare, NA934; 1:1000).

Article Title: Enhanced Expression and Functional Characterization of the Recombinant Putative Lysozyme-PMAP36 Fusion Protein
Article Snippet: .. The cell cultures at mid-logarithmic phase, OD600 of 0.2, were mixed with PBS (GE Healthcare) and PMAP36-P22 lysozyme fusion protein (final concentration: 64 μM) and incubated for 10 min at 37°C. .. We added EtBr (final concentration: 6 μM) to the reaction mixture and measured the fluorescence using fluorescence spectrometer (Infinite 200® Pro, TECAN, Austria); the excitation and emission wavelengths were 545 and 600 nm, respectively.

Article Title: Role of IRF4 in resistance to immunomodulatory (IMid) compounds® in Waldenström’s macroglobulinemia
Article Snippet: For the western blot analysis, whole cell proteic lysates were subjected to 4-12% sodium dodecyl sulfate polyacrylamide gel electrophoresis, transferred to nitrocellulose membrane (Thermo Fisher Scientific Inc., Waltham, MA) and incubated with antibodies directed against CRBN (kind gift from Dr H. Handa, Tokyo Medical University, Toko, Japan), IRF4, GAPDH (Santa Cruz Biotechnology, Santa Cruz, CA), β-actin (Sigma-Aldrich Co., St. Louis, MO), Ikaros and Aiolos (Cell Signaling, Beverly, MA). .. Quantification was done using ImageQuant TL software (GE Healthcare, Chicago, IL).

Article Title: Distinct roles of XPF-ERCC1 and Rad1-Rad10-Saw1 in replication-coupled and uncoupled inter-strand crosslink repair
Article Snippet: After a 15 min incubation at 30 °C, the reactions were loaded onto 10% native acrylamide gel and electrophoresed at 250 V in 1x TBE, for 45 min. .. Quantification of cleavage products was carried out using ImageQuant (GE).

Article Title: Cold-inducible RNA-binding protein CIRP/hnRNP A18 regulates telomerase activity in a temperature-dependent manner
Article Snippet: For EMSA, purified CIRP-GST proteins were incubated with 32 P labeled T7 capped TERC probe (Ambion) (please see Supplementary Materials for details regarding probe preparation). .. The products were resolved on 5% (wt/vol) native PAGE gel (100 V, 0.5x TBE, 180 min), visualized on a Typhoon PhosphorImager (General Electric Company) and quantified using ImageQuant (Amersham Biosciences).

Article Title: Anti-Sclerostin Antibody Inhibits Internalization of Sclerostin and Sclerostin-Mediated Antagonism of Wnt/LRP6 Signaling
Article Snippet: Lysates were incubated with the respective antisera for 2.5 hours and immune complexes were precipitated by adding protein A Sepharose (Amersham Biosciences). .. Bands were quantified using ImageQuant TL v2003.03 software (Amersham Biosciences).

Article Title: Corneal Decellularization: A Method of Recycling Unsuitable Donor Tissue for Clinical Translation?
Article Snippet: The corneas were initially decellularized using 10 mL of the following reagents; (i) hypertonic solution, 1.5 M NaCl in PBS; (ii) ionic-detergent, 0.5% w/v SDS in PBS (Amersham BioScience, Bucks, UK); or (iii) non-ionic detergent, 1% w/v Triton-X100 in PBS. .. All corneas were incubated at 4 °C with agitation for 24 h (SRT6 roller mixer, Stuart, SLS, Yorkshire, UK) followed by washing in 10 mL PBS (2 × 24 h with agitation).

Article Title: Activity, Abundance, and Localization of Quorum Sensing Receptors in Vibrio harveyi
Article Snippet: After incubation at 37°C for 15 min, membranes were collected by ultracentrifugation (100,000 × g , 15 min, 4°C). .. Quantification of fluorescence was performed using ImageQuant (GE Healthcare, München, Germany).

Activity Assay:

Article Title: Enhanced Expression and Functional Characterization of the Recombinant Putative Lysozyme-PMAP36 Fusion Protein
Article Snippet: Paragraph title: Outer membrane permeabilization activity ... The cell cultures at mid-logarithmic phase, OD600 of 0.2, were mixed with PBS (GE Healthcare) and PMAP36-P22 lysozyme fusion protein (final concentration: 64 μM) and incubated for 10 min at 37°C.

Article Title: Distinct roles of XPF-ERCC1 and Rad1-Rad10-Saw1 in replication-coupled and uncoupled inter-strand crosslink repair
Article Snippet: To test the effect of RPA on endonuclease activity, reactions were performed in RPA binding buffer (40 mM HEPES-KOH, pH 7.5, 75 mM KCl, 5 mM MgCl2 , 1 mM DTT, 5% glycerol, and 100 μg/ml BSA). .. Quantification of cleavage products was carried out using ImageQuant (GE).

BIA-KA:

Article Title: miR-204 Controls Glucagon-Like Peptide 1 Receptor Expression and Agonist Function
Article Snippet: Protein concentrations were measured by Pierce BCA Protein Assay (Thermo Fisher Scientific), and equal amounts of protein were loaded. .. The following antibodies were used: rabbit anti-GLP1R IgG (sc-66911; Santa Cruz Biotechnology, Dallas, TX), mouse anti-actin IgG (ab3280; Abcam), goat anti-rabbit IgG (sc-2004; Santa Cruz Biotechnology), and goat anti-mouse IgG (sc-2005; Santa Cruz Biotechnology), and bands were visualized by ECL Plus (GE Healthcare, Little Chalfont, U.K.) and quantified by ImageQuant TL software (GE Healthcare).

Acrylamide Gel Assay:

Article Title: Distinct roles of XPF-ERCC1 and Rad1-Rad10-Saw1 in replication-coupled and uncoupled inter-strand crosslink repair
Article Snippet: After a 15 min incubation at 30 °C, the reactions were loaded onto 10% native acrylamide gel and electrophoresed at 250 V in 1x TBE, for 45 min. .. Quantification of cleavage products was carried out using ImageQuant (GE).

Western Blot:

Article Title: Role of IRF4 in resistance to immunomodulatory (IMid) compounds® in Waldenström’s macroglobulinemia
Article Snippet: Paragraph title: Western blotting, apoptosis and viability assays ... Quantification was done using ImageQuant TL software (GE Healthcare, Chicago, IL).

Article Title: JMJD1C Exhibits Multiple Functions in Epigenetic Regulation during Spermatogenesis
Article Snippet: .. The blotted membranes (Immobilon-XP) were immunoreacted with primary antibodies and visualized with the ECL Prime Western blotting Detection kit (GE Healthcare) containing secondary antibodies, HRP (horseradish peroxidase)-labeled anti-mouse IgG and anti-rabbit IgG, and the Quant LAS4000-mini (GE Healthcare) in accordance with the manufacturer’s instruction. .. The antibodies used for immunofluorescence and immunoblotting detection are listed in .

Recombinase Polymerase Amplification:

Article Title: Distinct roles of XPF-ERCC1 and Rad1-Rad10-Saw1 in replication-coupled and uncoupled inter-strand crosslink repair
Article Snippet: Yeast RPA (80 nm) was pre-incubated with the 3′ flap substrate for 10 min at 30 °C. .. Quantification of cleavage products was carried out using ImageQuant (GE).

Hybridization:

Article Title: Host Pah1p phosphatidate phosphatase limits viral replication by regulating phospholipid synthesis
Article Snippet: P32 -labled probes specific to BMV positive- or negative-strand RNA or 18S rRNA were used in the hybridization. .. Radioactive signals were scanned using a Typhoon FLA 7000 phosphoimager and the intensity of radioactive signals were quantified by using ImageQuant TL (GE healthcare).

Immunohistochemistry:

Article Title: JMJD1C Exhibits Multiple Functions in Epigenetic Regulation during Spermatogenesis
Article Snippet: Paragraph title: Immunohistochemical staining, X-gal staining and immunoblotting analysis ... The blotted membranes (Immobilon-XP) were immunoreacted with primary antibodies and visualized with the ECL Prime Western blotting Detection kit (GE Healthcare) containing secondary antibodies, HRP (horseradish peroxidase)-labeled anti-mouse IgG and anti-rabbit IgG, and the Quant LAS4000-mini (GE Healthcare) in accordance with the manufacturer’s instruction.

Northern Blot:

Article Title: The output of protein-coding genes shifts to circular RNAs when the pre-mRNA processing machinery is limiting
Article Snippet: Paragraph title: Northern blotting ... Blots were viewed and quantified with the Typhoon 9500 scanner (GE Healthcare) and quantified using ImageQuant (GE Healthcare).

Article Title: Host Pah1p phosphatidate phosphatase limits viral replication by regulating phospholipid synthesis
Article Snippet: Paragraph title: RNA extraction and Northern blotting ... Radioactive signals were scanned using a Typhoon FLA 7000 phosphoimager and the intensity of radioactive signals were quantified by using ImageQuant TL (GE healthcare).

Imaging:

Article Title: Anti-Sclerostin Antibody Inhibits Internalization of Sclerostin and Sclerostin-Mediated Antagonism of Wnt/LRP6 Signaling
Article Snippet: Gels were dried and scanned with the STORM imaging system (Amersham Biosciences). .. Bands were quantified using ImageQuant TL v2003.03 software (Amersham Biosciences).

Binding Assay:

Article Title: Distinct roles of XPF-ERCC1 and Rad1-Rad10-Saw1 in replication-coupled and uncoupled inter-strand crosslink repair
Article Snippet: To test the effect of RPA on endonuclease activity, reactions were performed in RPA binding buffer (40 mM HEPES-KOH, pH 7.5, 75 mM KCl, 5 mM MgCl2 , 1 mM DTT, 5% glycerol, and 100 μg/ml BSA). .. Quantification of cleavage products was carried out using ImageQuant (GE).

Article Title: Cold-inducible RNA-binding protein CIRP/hnRNP A18 regulates telomerase activity in a temperature-dependent manner
Article Snippet: Binding reactions were performed at 25°C for 30 min in binding buffer (100 mM NaCl, 10 mMTris (pH7.5), 5% (w/v) glycerol, 1 mM MgCl2 , 1 U of RNasin Ribonuclease Inhibitor (Promega) and 0.3 pmol of 32 P-labeled TERC probe). .. The products were resolved on 5% (wt/vol) native PAGE gel (100 V, 0.5x TBE, 180 min), visualized on a Typhoon PhosphorImager (General Electric Company) and quantified using ImageQuant (Amersham Biosciences).

Article Title: Anti-Sclerostin Antibody Inhibits Internalization of Sclerostin and Sclerostin-Mediated Antagonism of Wnt/LRP6 Signaling
Article Snippet: Paragraph title: [125 I]Sclerostin Binding Assay ... Bands were quantified using ImageQuant TL v2003.03 software (Amersham Biosciences).

Immunofluorescence:

Article Title: JMJD1C Exhibits Multiple Functions in Epigenetic Regulation during Spermatogenesis
Article Snippet: The blotted membranes (Immobilon-XP) were immunoreacted with primary antibodies and visualized with the ECL Prime Western blotting Detection kit (GE Healthcare) containing secondary antibodies, HRP (horseradish peroxidase)-labeled anti-mouse IgG and anti-rabbit IgG, and the Quant LAS4000-mini (GE Healthcare) in accordance with the manufacturer’s instruction. .. The antibodies used for immunofluorescence and immunoblotting detection are listed in .

Fluorescence:

Article Title: Enhanced Expression and Functional Characterization of the Recombinant Putative Lysozyme-PMAP36 Fusion Protein
Article Snippet: The cell cultures at mid-logarithmic phase, OD600 of 0.2, were mixed with PBS (GE Healthcare) and PMAP36-P22 lysozyme fusion protein (final concentration: 64 μM) and incubated for 10 min at 37°C. .. We added EtBr (final concentration: 6 μM) to the reaction mixture and measured the fluorescence using fluorescence spectrometer (Infinite 200® Pro, TECAN, Austria); the excitation and emission wavelengths were 545 and 600 nm, respectively.

Article Title: Activity, Abundance, and Localization of Quorum Sensing Receptors in Vibrio harveyi
Article Snippet: .. Quantification of fluorescence was performed using ImageQuant (GE Healthcare, München, Germany). .. Fluorescence microscopy Cells were grown in AB medium until the late exponential growth phase and fluorescence microscopy was conducted.

Flow Cytometry:

Article Title: Role of IRF4 in resistance to immunomodulatory (IMid) compounds® in Waldenström’s macroglobulinemia
Article Snippet: Staining was detected and analyzed on a CyAn ADP analyzer flow cytometer (Beckman Coulter Inc.). .. Quantification was done using ImageQuant TL software (GE Healthcare, Chicago, IL).

Labeling:

Article Title: Cold-inducible RNA-binding protein CIRP/hnRNP A18 regulates telomerase activity in a temperature-dependent manner
Article Snippet: For EMSA, purified CIRP-GST proteins were incubated with 32 P labeled T7 capped TERC probe (Ambion) (please see Supplementary Materials for details regarding probe preparation). .. The products were resolved on 5% (wt/vol) native PAGE gel (100 V, 0.5x TBE, 180 min), visualized on a Typhoon PhosphorImager (General Electric Company) and quantified using ImageQuant (Amersham Biosciences).

Purification:

Article Title: Cold-inducible RNA-binding protein CIRP/hnRNP A18 regulates telomerase activity in a temperature-dependent manner
Article Snippet: For EMSA, purified CIRP-GST proteins were incubated with 32 P labeled T7 capped TERC probe (Ambion) (please see Supplementary Materials for details regarding probe preparation). .. The products were resolved on 5% (wt/vol) native PAGE gel (100 V, 0.5x TBE, 180 min), visualized on a Typhoon PhosphorImager (General Electric Company) and quantified using ImageQuant (Amersham Biosciences).

Protein Purification:

Article Title: Cold-inducible RNA-binding protein CIRP/hnRNP A18 regulates telomerase activity in a temperature-dependent manner
Article Snippet: Paragraph title: Protein purification and electrophoretic mobility shift assay (EMSA) ... The products were resolved on 5% (wt/vol) native PAGE gel (100 V, 0.5x TBE, 180 min), visualized on a Typhoon PhosphorImager (General Electric Company) and quantified using ImageQuant (Amersham Biosciences).

Electrophoretic Mobility Shift Assay:

Article Title: Cold-inducible RNA-binding protein CIRP/hnRNP A18 regulates telomerase activity in a temperature-dependent manner
Article Snippet: Paragraph title: Protein purification and electrophoretic mobility shift assay (EMSA) ... The products were resolved on 5% (wt/vol) native PAGE gel (100 V, 0.5x TBE, 180 min), visualized on a Typhoon PhosphorImager (General Electric Company) and quantified using ImageQuant (Amersham Biosciences).

Polyacrylamide Gel Electrophoresis:

Article Title: Role of IRF4 in resistance to immunomodulatory (IMid) compounds® in Waldenström’s macroglobulinemia
Article Snippet: For the western blot analysis, whole cell proteic lysates were subjected to 4-12% sodium dodecyl sulfate polyacrylamide gel electrophoresis, transferred to nitrocellulose membrane (Thermo Fisher Scientific Inc., Waltham, MA) and incubated with antibodies directed against CRBN (kind gift from Dr H. Handa, Tokyo Medical University, Toko, Japan), IRF4, GAPDH (Santa Cruz Biotechnology, Santa Cruz, CA), β-actin (Sigma-Aldrich Co., St. Louis, MO), Ikaros and Aiolos (Cell Signaling, Beverly, MA). .. Quantification was done using ImageQuant TL software (GE Healthcare, Chicago, IL).

SDS Page:

Article Title: JMJD1C Exhibits Multiple Functions in Epigenetic Regulation during Spermatogenesis
Article Snippet: For the immunoblotting analysis, testicular cell extracts (15 μg per lane) were separated by SDS-PAGE. .. The blotted membranes (Immobilon-XP) were immunoreacted with primary antibodies and visualized with the ECL Prime Western blotting Detection kit (GE Healthcare) containing secondary antibodies, HRP (horseradish peroxidase)-labeled anti-mouse IgG and anti-rabbit IgG, and the Quant LAS4000-mini (GE Healthcare) in accordance with the manufacturer’s instruction.

Article Title: Anti-Sclerostin Antibody Inhibits Internalization of Sclerostin and Sclerostin-Mediated Antagonism of Wnt/LRP6 Signaling
Article Snippet: Samples were washed, boiled in SDS sample buffer and subjected to SDS-PAGE. .. Bands were quantified using ImageQuant TL v2003.03 software (Amersham Biosciences).

Article Title: Activity, Abundance, and Localization of Quorum Sensing Receptors in Vibrio harveyi
Article Snippet: SDS-PAGE was performed according to Laemmli (Laemmli, ) and run at 150 V for 2 h in the dark to avoid bleaching of the fluorophore. .. Quantification of fluorescence was performed using ImageQuant (GE Healthcare, München, Germany).

Software:

Article Title: miR-204 Controls Glucagon-Like Peptide 1 Receptor Expression and Agonist Function
Article Snippet: .. The following antibodies were used: rabbit anti-GLP1R IgG (sc-66911; Santa Cruz Biotechnology, Dallas, TX), mouse anti-actin IgG (ab3280; Abcam), goat anti-rabbit IgG (sc-2004; Santa Cruz Biotechnology), and goat anti-mouse IgG (sc-2005; Santa Cruz Biotechnology), and bands were visualized by ECL Plus (GE Healthcare, Little Chalfont, U.K.) and quantified by ImageQuant TL software (GE Healthcare). ..

Article Title: Role of IRF4 in resistance to immunomodulatory (IMid) compounds® in Waldenström’s macroglobulinemia
Article Snippet: .. Quantification was done using ImageQuant TL software (GE Healthcare, Chicago, IL). .. Quantitative RT-PCR Total RNA was purified (Nucleospin RNA isolation kit, Macherey-Nagel GmbH, Düren, Germany) and then reverse transcribed into cDNA using the Superscript® VILO cDNA synthesis kit (Thermo Fisher Scientific Inc.) following the manufacturer's instructions.

Article Title: JMJD1C Exhibits Multiple Functions in Epigenetic Regulation during Spermatogenesis
Article Snippet: Fluorescent immunohistochemical images were obtained with an Olympus IX71 and the MetaMorph software. .. The blotted membranes (Immobilon-XP) were immunoreacted with primary antibodies and visualized with the ECL Prime Western blotting Detection kit (GE Healthcare) containing secondary antibodies, HRP (horseradish peroxidase)-labeled anti-mouse IgG and anti-rabbit IgG, and the Quant LAS4000-mini (GE Healthcare) in accordance with the manufacturer’s instruction.

Article Title: Anti-Sclerostin Antibody Inhibits Internalization of Sclerostin and Sclerostin-Mediated Antagonism of Wnt/LRP6 Signaling
Article Snippet: .. Bands were quantified using ImageQuant TL v2003.03 software (Amersham Biosciences). ..

RNA Extraction:

Article Title: Host Pah1p phosphatidate phosphatase limits viral replication by regulating phospholipid synthesis
Article Snippet: Paragraph title: RNA extraction and Northern blotting ... Radioactive signals were scanned using a Typhoon FLA 7000 phosphoimager and the intensity of radioactive signals were quantified by using ImageQuant TL (GE healthcare).

Recombinant:

Article Title: Anti-Sclerostin Antibody Inhibits Internalization of Sclerostin and Sclerostin-Mediated Antagonism of Wnt/LRP6 Signaling
Article Snippet: [125 I]Sclerostin Binding Assay Iodination of recombinant murine full-length Sclerostin was performed according to the chloramine T method and cells were subsequently affinity-labeled with the radioactive ligand as described before [57,58]. .. Bands were quantified using ImageQuant TL v2003.03 software (Amersham Biosciences).

Spectrophotometry:

Article Title: Decreased Plasma Aβ in Hyperlipidemic APPSL Transgenic Mice Is Associated with BBB Dysfunction
Article Snippet: After 30 min incubation in the dark, reaction was stopped with 100 μl 0.5 M H2 SO4 and measured at 450 nm with μQuant universal microplate spectrophotometer. .. Antibodies: anti-mouse IgG biotinylated (Eubio, BA-2000; 1:5000) + anti-streptavidin HRP conjugated (Zymed, 43-4323; 1:5000); anti-occludin (abcam, ab167161; 1:1000) + anti-rabbit IgG, HRP-linked (GE-Healthcare, NA934; 1:5000); anti-ZO1 tight junction protein (abcam, ab59720; 1:100) + anti-rabbit IgG, HRP-linked (GE-Healthcare, NA934; 1:2500); anti-Vascular Cell Adhesion Molecule 1 (VCAM-1) (Santa Cruz, sc-1504-R; 1:500) + anti-rabbit IgG, HRP-linked (GE-Healthcare, NA934; 1:5000); anti-LRP1 [alpha-2-macroglobulin receptor] (Santa Cruz, sc-57351; 1:500) + anti-mouse IgG, peroxidase linked (GE-Healthcare, NA931; 1:1000); anti-Cluster of Differentiation 31 (CD31) (abcam, ab28364; 1:250) + anti-rabbit IgG, HRP-linked (GE-Healthcare, NA934; 1:1000).

Concentration Assay:

Article Title: Enhanced Expression and Functional Characterization of the Recombinant Putative Lysozyme-PMAP36 Fusion Protein
Article Snippet: .. The cell cultures at mid-logarithmic phase, OD600 of 0.2, were mixed with PBS (GE Healthcare) and PMAP36-P22 lysozyme fusion protein (final concentration: 64 μM) and incubated for 10 min at 37°C. .. We added EtBr (final concentration: 6 μM) to the reaction mixture and measured the fluorescence using fluorescence spectrometer (Infinite 200® Pro, TECAN, Austria); the excitation and emission wavelengths were 545 and 600 nm, respectively.

Staining:

Article Title: Role of IRF4 in resistance to immunomodulatory (IMid) compounds® in Waldenström’s macroglobulinemia
Article Snippet: Staining was detected and analyzed on a CyAn ADP analyzer flow cytometer (Beckman Coulter Inc.). .. Quantification was done using ImageQuant TL software (GE Healthcare, Chicago, IL).

Article Title: JMJD1C Exhibits Multiple Functions in Epigenetic Regulation during Spermatogenesis
Article Snippet: Paragraph title: Immunohistochemical staining, X-gal staining and immunoblotting analysis ... The blotted membranes (Immobilon-XP) were immunoreacted with primary antibodies and visualized with the ECL Prime Western blotting Detection kit (GE Healthcare) containing secondary antibodies, HRP (horseradish peroxidase)-labeled anti-mouse IgG and anti-rabbit IgG, and the Quant LAS4000-mini (GE Healthcare) in accordance with the manufacturer’s instruction.

Clear Native PAGE:

Article Title: Cold-inducible RNA-binding protein CIRP/hnRNP A18 regulates telomerase activity in a temperature-dependent manner
Article Snippet: .. The products were resolved on 5% (wt/vol) native PAGE gel (100 V, 0.5x TBE, 180 min), visualized on a Typhoon PhosphorImager (General Electric Company) and quantified using ImageQuant (Amersham Biosciences). .. Telomeric restriction fragment (TRF) analysis Average length of telomeres was determined using the TRF assay analysis as described previously ( ).

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    GE Healthcare general electric image quant las 4000 mini scanner
    General Electric Image Quant Las 4000 Mini Scanner, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/general electric image quant las 4000 mini scanner/product/GE Healthcare
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    general electric image quant las 4000 mini scanner - by Bioz Stars, 2020-04
    92/100 stars
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