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Affibody imac columns
Imac Columns, supplied by Affibody, used in various techniques. Bioz Stars score: 81/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/imac columns/product/Affibody
Average 81 stars, based on 1 article reviews
Price from $9.99 to $1999.99
imac columns - by Bioz Stars, 2020-02
81/100 stars

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Related Articles

Flow Cytometry:

Article Title: Preclinical PET imaging of EGFR levels: pairing a targeting with a non-targeting Sel-tagged Affibody-based tracer to estimate the specific uptake
Article Snippet: .. Cleavage mixtures were applied on IMAC columns, and Affibody molecules were recovered in the flow-through. ..

Polymerase Chain Reaction:

Article Title: Preclinical PET imaging of EGFR levels: pairing a targeting with a non-targeting Sel-tagged Affibody-based tracer to estimate the specific uptake
Article Snippet: Gene segments encoding a hexahistidyl tag (H6 ), GFP, and a cleavage site for TEV-protease were introduced upstream of the Affibody molecules using polymerase chain reaction (PCR), to encode the constructs H6 -GFP-TEV-ZEGFR:2377 -ST and H6 -GFP-TEV-ZTaq:3638 -ST. Sequence-confirmed plasmids were transformed to BL21(DE3) cells (Novagen) already harboring the pSUABC plasmid [ ]. .. Cleavage mixtures were applied on IMAC columns, and Affibody molecules were recovered in the flow-through.

Construct:

Article Title: Preclinical PET imaging of EGFR levels: pairing a targeting with a non-targeting Sel-tagged Affibody-based tracer to estimate the specific uptake
Article Snippet: Gene segments encoding a hexahistidyl tag (H6 ), GFP, and a cleavage site for TEV-protease were introduced upstream of the Affibody molecules using polymerase chain reaction (PCR), to encode the constructs H6 -GFP-TEV-ZEGFR:2377 -ST and H6 -GFP-TEV-ZTaq:3638 -ST. Sequence-confirmed plasmids were transformed to BL21(DE3) cells (Novagen) already harboring the pSUABC plasmid [ ]. .. Cleavage mixtures were applied on IMAC columns, and Affibody molecules were recovered in the flow-through.

Purification:

Article Title: Preclinical PET imaging of EGFR levels: pairing a targeting with a non-targeting Sel-tagged Affibody-based tracer to estimate the specific uptake
Article Snippet: The two fusion proteins were purified by IMAC on a HisTrap FF column (GE Healthcare) and eluted with 50 % tris(hydroxymethyl)aminomethane hydrochloride (Tris)-HCl buffer, 50 mM, pH 8.0, containing 500 mM imidazole. .. Cleavage mixtures were applied on IMAC columns, and Affibody molecules were recovered in the flow-through.

Concentration Assay:

Article Title: Preclinical PET imaging of EGFR levels: pairing a targeting with a non-targeting Sel-tagged Affibody-based tracer to estimate the specific uptake
Article Snippet: Gene expression was induced by adding isopropyl β-D-1-thiogalactopyranoside to a final concentration of 0.5 mM. l -Cysteine and selenite were added to the cultures to a final concentration of 1 μM and 5 nM, respectively, and cultivations were continued overnight at 25 °C. .. Cleavage mixtures were applied on IMAC columns, and Affibody molecules were recovered in the flow-through.

Expressing:

Article Title: Preclinical PET imaging of EGFR levels: pairing a targeting with a non-targeting Sel-tagged Affibody-based tracer to estimate the specific uptake
Article Snippet: Paragraph title: DNA constructions and expression of Sel-tagged Affibody molecules ... Cleavage mixtures were applied on IMAC columns, and Affibody molecules were recovered in the flow-through.

Sequencing:

Article Title: Preclinical PET imaging of EGFR levels: pairing a targeting with a non-targeting Sel-tagged Affibody-based tracer to estimate the specific uptake
Article Snippet: Gene segments encoding a hexahistidyl tag (H6 ), GFP, and a cleavage site for TEV-protease were introduced upstream of the Affibody molecules using polymerase chain reaction (PCR), to encode the constructs H6 -GFP-TEV-ZEGFR:2377 -ST and H6 -GFP-TEV-ZTaq:3638 -ST. Sequence-confirmed plasmids were transformed to BL21(DE3) cells (Novagen) already harboring the pSUABC plasmid [ ]. .. Cleavage mixtures were applied on IMAC columns, and Affibody molecules were recovered in the flow-through.

Reversed-phase Chromatography:

Article Title: Preclinical PET imaging of EGFR levels: pairing a targeting with a non-targeting Sel-tagged Affibody-based tracer to estimate the specific uptake
Article Snippet: Cleavage mixtures were applied on IMAC columns, and Affibody molecules were recovered in the flow-through. .. ZEGFR:2377 -ST and ZTaq:3638 -ST were further purified by reversed phase chromatography using a Resource RPC 1-ml column (GE Healthcare), using the following buffer system: buffer A, 0.1 % trifluoroacetic acid (TFA) in water, and buffer B, 0.1 % TFA in acetonitrile.

Transformation Assay:

Article Title: Preclinical PET imaging of EGFR levels: pairing a targeting with a non-targeting Sel-tagged Affibody-based tracer to estimate the specific uptake
Article Snippet: Gene segments encoding a hexahistidyl tag (H6 ), GFP, and a cleavage site for TEV-protease were introduced upstream of the Affibody molecules using polymerase chain reaction (PCR), to encode the constructs H6 -GFP-TEV-ZEGFR:2377 -ST and H6 -GFP-TEV-ZTaq:3638 -ST. Sequence-confirmed plasmids were transformed to BL21(DE3) cells (Novagen) already harboring the pSUABC plasmid [ ]. .. Cleavage mixtures were applied on IMAC columns, and Affibody molecules were recovered in the flow-through.

Plasmid Preparation:

Article Title: Preclinical PET imaging of EGFR levels: pairing a targeting with a non-targeting Sel-tagged Affibody-based tracer to estimate the specific uptake
Article Snippet: Gene segments encoding a hexahistidyl tag (H6 ), GFP, and a cleavage site for TEV-protease were introduced upstream of the Affibody molecules using polymerase chain reaction (PCR), to encode the constructs H6 -GFP-TEV-ZEGFR:2377 -ST and H6 -GFP-TEV-ZTaq:3638 -ST. Sequence-confirmed plasmids were transformed to BL21(DE3) cells (Novagen) already harboring the pSUABC plasmid [ ]. .. Cleavage mixtures were applied on IMAC columns, and Affibody molecules were recovered in the flow-through.

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    Affibody imac columns
    Imac Columns, supplied by Affibody, used in various techniques. Bioz Stars score: 81/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/imac columns/product/Affibody
    Average 81 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    imac columns - by Bioz Stars, 2020-02
    81/100 stars
      Buy from Supplier

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