il 27  (R&D Systems)

 
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    Name:
    Recombinant Human IL 27 Protein
    Description:
    The Recombinant Human IL 27 Protein from R D Systems is derived from NS0 The Recombinant Human IL 27 Protein has been validated for the following applications Bioactivity
    Catalog Number:
    2526-IL-010
    Price:
    329
    Category:
    Proteins and Enzymes
    Source:
    NS0-derived Recombinant Human IL-27 Protein
    Applications:
    Bioactivity
    Purity:
    >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.
    Conjugate:
    Unconjugated
    Size:
    10 ug
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    Structured Review

    R&D Systems il 27
    Circulating <t>IL-27</t> levels are elevated in neonates. ( a ) Blood was collected from mice in the indicated age groups and serum levels of IL-27 were measured by luminescent immunoassay. Each symbol represents an individual animal finding. Statistical significance was determined using unpaired t tests with Welch’s correction.
    The Recombinant Human IL 27 Protein from R D Systems is derived from NS0 The Recombinant Human IL 27 Protein has been validated for the following applications Bioactivity
    https://www.bioz.com/result/il 27/product/R&D Systems
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    il 27 - by Bioz Stars, 2021-05
    93/100 stars

    Images

    1) Product Images from "Murine myeloid-derived suppressor cells are a source of elevated levels of interleukin-27 in early life and compromise control of bacterial infection"

    Article Title: Murine myeloid-derived suppressor cells are a source of elevated levels of interleukin-27 in early life and compromise control of bacterial infection

    Journal: Immunology and cell biology

    doi: 10.1111/imcb.12224

    Circulating IL-27 levels are elevated in neonates. ( a ) Blood was collected from mice in the indicated age groups and serum levels of IL-27 were measured by luminescent immunoassay. Each symbol represents an individual animal finding. Statistical significance was determined using unpaired t tests with Welch’s correction.
    Figure Legend Snippet: Circulating IL-27 levels are elevated in neonates. ( a ) Blood was collected from mice in the indicated age groups and serum levels of IL-27 were measured by luminescent immunoassay. Each symbol represents an individual animal finding. Statistical significance was determined using unpaired t tests with Welch’s correction.

    Techniques Used: Mouse Assay

    Isolated MDSCs produce IL-27. MDSCs were isolated from neonatal C57BL/6 mouse spleens as described in the Methods . ( a ) The isolated cells were labeled for surface-expressed Gr-1 and intracellular IL-27 and analyzed by flow cytometry. A representative dot plot relative to isotype controls is shown. ( b ) MDSC expression of the indicated genes relative to a no template control was determined by real time PCR. Six independent measurements using MDSCs obtained from pups ranging 5–8 days of age are combined and shown. ( c ) IL-27 concentration was determined in 24 h MDSC culture supernatants by ELISA. Seven independent measurements using MDSCs obtained from pups ranging 5–8 days of age are combined and shown. ( d ) The nitrite concentration in 48 h MDSC culture supernatants was measured. Five independent measurements using MDSCs obtained from pups ranging 5–10 days of age are combined and shown. ( e ) Arginase activity was measured relative to purified standards. Eight independent measurements using MDSCs obtained from pups ranging 5–8 days of age are combined and shown. ( f ) Oxidative burst was measured in MDSCs using Dihydrorhodamine 123. Data using MDSCs obtained from six day old pups representative of three separate measurements is shown.
    Figure Legend Snippet: Isolated MDSCs produce IL-27. MDSCs were isolated from neonatal C57BL/6 mouse spleens as described in the Methods . ( a ) The isolated cells were labeled for surface-expressed Gr-1 and intracellular IL-27 and analyzed by flow cytometry. A representative dot plot relative to isotype controls is shown. ( b ) MDSC expression of the indicated genes relative to a no template control was determined by real time PCR. Six independent measurements using MDSCs obtained from pups ranging 5–8 days of age are combined and shown. ( c ) IL-27 concentration was determined in 24 h MDSC culture supernatants by ELISA. Seven independent measurements using MDSCs obtained from pups ranging 5–8 days of age are combined and shown. ( d ) The nitrite concentration in 48 h MDSC culture supernatants was measured. Five independent measurements using MDSCs obtained from pups ranging 5–10 days of age are combined and shown. ( e ) Arginase activity was measured relative to purified standards. Eight independent measurements using MDSCs obtained from pups ranging 5–8 days of age are combined and shown. ( f ) Oxidative burst was measured in MDSCs using Dihydrorhodamine 123. Data using MDSCs obtained from six day old pups representative of three separate measurements is shown.

    Techniques Used: Isolation, Labeling, Flow Cytometry, Expressing, Real-time Polymerase Chain Reaction, Concentration Assay, Enzyme-linked Immunosorbent Assay, Activity Assay, Purification

    Gr-1 + cells that produce IL-27 are more abundant in neonates. Splenocytes from C57BL/6 mice at the indicated age were labeled for surface-expressed Gr-1 and intracellular IL-27 and analyzed by flow cytometry. ( a ) Representative dot plots relative to isotype controls are shown. ( b ) The combined percent Gr-1 + IL-27 + population from at least 8 mice at each age group are shown. Statistical significance was determined using an ANOVA and Tukey’s multiple comparison’s test.
    Figure Legend Snippet: Gr-1 + cells that produce IL-27 are more abundant in neonates. Splenocytes from C57BL/6 mice at the indicated age were labeled for surface-expressed Gr-1 and intracellular IL-27 and analyzed by flow cytometry. ( a ) Representative dot plots relative to isotype controls are shown. ( b ) The combined percent Gr-1 + IL-27 + population from at least 8 mice at each age group are shown. Statistical significance was determined using an ANOVA and Tukey’s multiple comparison’s test.

    Techniques Used: Mouse Assay, Labeling, Flow Cytometry

    MDSCs compromise macrophage control of E. coli . Bone marrow-derived macrophages were infected with Escherichia coli at an MOI of 20 in the presence or absence of MDSCs (5–13 days of age) at the indicated ratio. Recovery of intracellular bacteria was enumerated at 18 h and shown as mean log 10 recovered colony forming units ± standard error ( a ). Statistical significance in a combined six experiments was determined using a paired samples t test . ( b ) MDSCs were added to cultures where shown at the indicated ratio to macrophages. A combined four experiments are shown. Statistical significance was determined using paired samples t tests . ( c ) Macrophages were cultured as indicated ± MDSCs (ratio of 1:1 with macrophages) in the presence or absence of a soluble receptor to neutralize IL-27 (sIL27R). Five combined experiments are shown. Statistical significance was determined using paired samples t tests . ( d ) The concentration of TNF-α in culture supernatants was determined by ELISA. The presence of bacteria (Ec), sIL-27R, and ratio of MDSCs to macrophages (0.1X or 1X) is indicated. Results from an individual experiment representative of multiple are shown.
    Figure Legend Snippet: MDSCs compromise macrophage control of E. coli . Bone marrow-derived macrophages were infected with Escherichia coli at an MOI of 20 in the presence or absence of MDSCs (5–13 days of age) at the indicated ratio. Recovery of intracellular bacteria was enumerated at 18 h and shown as mean log 10 recovered colony forming units ± standard error ( a ). Statistical significance in a combined six experiments was determined using a paired samples t test . ( b ) MDSCs were added to cultures where shown at the indicated ratio to macrophages. A combined four experiments are shown. Statistical significance was determined using paired samples t tests . ( c ) Macrophages were cultured as indicated ± MDSCs (ratio of 1:1 with macrophages) in the presence or absence of a soluble receptor to neutralize IL-27 (sIL27R). Five combined experiments are shown. Statistical significance was determined using paired samples t tests . ( d ) The concentration of TNF-α in culture supernatants was determined by ELISA. The presence of bacteria (Ec), sIL-27R, and ratio of MDSCs to macrophages (0.1X or 1X) is indicated. Results from an individual experiment representative of multiple are shown.

    Techniques Used: Derivative Assay, Infection, Cell Culture, Concentration Assay, Enzyme-linked Immunosorbent Assay

    2) Product Images from "PD-L1/PD-1 Pattern of Expression Within the Bone Marrow Immune Microenvironment in Smoldering Myeloma and Active Multiple Myeloma Patients"

    Article Title: PD-L1/PD-1 Pattern of Expression Within the Bone Marrow Immune Microenvironment in Smoldering Myeloma and Active Multiple Myeloma Patients

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2020.613007

    Anti-tumoral IL-27 BM serum levels are inversely correlated with PD-L1 expression on monocytes and %CD8 + PD-1 + cells. Dot plots represent median BM serum levels of IL-10 (A) , IL-6 (B) , and IL-27 (C) measured by ELISA in a subgroup of patients at different stages of disease ( p values calculated by Mann-Whitney test). (D) The graph shows a significant inverse correlation (r: −0.3886) between PD-L1 MFI on CD14+ cells and IL-27 BM serum levels in a total cohort of 33 patients with SMM and active MM. (E) IL-27 BM serum levels are inversely correlated with %CD8 + PD-1 + cells (r: −0.4292). (F) Inverse correlation between anti-tumoral IL-27 levels and immune-suppressive IL-10 is shown in the graph (r: −0.4292). ( p values calculated by Spearman’s correlation). ns, not significant.
    Figure Legend Snippet: Anti-tumoral IL-27 BM serum levels are inversely correlated with PD-L1 expression on monocytes and %CD8 + PD-1 + cells. Dot plots represent median BM serum levels of IL-10 (A) , IL-6 (B) , and IL-27 (C) measured by ELISA in a subgroup of patients at different stages of disease ( p values calculated by Mann-Whitney test). (D) The graph shows a significant inverse correlation (r: −0.3886) between PD-L1 MFI on CD14+ cells and IL-27 BM serum levels in a total cohort of 33 patients with SMM and active MM. (E) IL-27 BM serum levels are inversely correlated with %CD8 + PD-1 + cells (r: −0.4292). (F) Inverse correlation between anti-tumoral IL-27 levels and immune-suppressive IL-10 is shown in the graph (r: −0.4292). ( p values calculated by Spearman’s correlation). ns, not significant.

    Techniques Used: Expressing, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

    3) Product Images from "Interleukin-27 inhibition of HIV-1 involves an intermediate induction of type I interferon"

    Article Title: Interleukin-27 inhibition of HIV-1 involves an intermediate induction of type I interferon

    Journal:

    doi: 10.1182/blood-2009-03-211540

    IL-27 inhibits HIV infection in macrophages . (A) Monocyte-derived macrophages were infected with 10 3 TCID 50 /mL BaL for 2 hours. Cells were washed, 10% DMEM added, and IL-27 at 0, 1, 10, and 100 ng/mL added only once after infection. Cells were fed every
    Figure Legend Snippet: IL-27 inhibits HIV infection in macrophages . (A) Monocyte-derived macrophages were infected with 10 3 TCID 50 /mL BaL for 2 hours. Cells were washed, 10% DMEM added, and IL-27 at 0, 1, 10, and 100 ng/mL added only once after infection. Cells were fed every

    Techniques Used: Infection, Derivative Assay

    IFN-α up-regulates IL-27 and IL-27 receptor mRNA expression . (A) Monocyte-derived macrophages (n = 3) were stimulated with IFN-α (10 ng/mL, 4 hours) in parallel with mock-treated cultures, and RNA was extracted and processed for microarray
    Figure Legend Snippet: IFN-α up-regulates IL-27 and IL-27 receptor mRNA expression . (A) Monocyte-derived macrophages (n = 3) were stimulated with IFN-α (10 ng/mL, 4 hours) in parallel with mock-treated cultures, and RNA was extracted and processed for microarray

    Techniques Used: Expressing, Derivative Assay, Microarray

    IL-27 induction of IFN-α and IFN-β supports APOBEC3A/G expression and inhibition of HIV . (A) PBMC were treated with IL-27 at 100 ng/mL for 30 minutes to 24 hours. RNA was reverse transcribed to cDNA, and amplification was performed using
    Figure Legend Snippet: IL-27 induction of IFN-α and IFN-β supports APOBEC3A/G expression and inhibition of HIV . (A) PBMC were treated with IL-27 at 100 ng/mL for 30 minutes to 24 hours. RNA was reverse transcribed to cDNA, and amplification was performed using

    Techniques Used: Expressing, Inhibition, Amplification

    IL-27 enhances macrophage APOBEC RNA expression . Monocyte-derived macrophages were treated 18 to 24 hours with IL-27 at 100 ng/mL or IFN-α at 10 ng/mL. Cells were lysed and processed for RNA following QIAGEN's RNeasy protocol, and gene transcription
    Figure Legend Snippet: IL-27 enhances macrophage APOBEC RNA expression . Monocyte-derived macrophages were treated 18 to 24 hours with IL-27 at 100 ng/mL or IFN-α at 10 ng/mL. Cells were lysed and processed for RNA following QIAGEN's RNeasy protocol, and gene transcription

    Techniques Used: RNA Expression, Derivative Assay

    IL-27 inhibition of T-cell HIV includes an IFN intermediate . (A) IL-27 induction of IFN- α in T cells at 0.5 to 4 hours (4 hours shown; n = 2), without corresponding increase in APOBEC3A until 24 hours but no increase in APOBEC3G (inset; n = 3).
    Figure Legend Snippet: IL-27 inhibition of T-cell HIV includes an IFN intermediate . (A) IL-27 induction of IFN- α in T cells at 0.5 to 4 hours (4 hours shown; n = 2), without corresponding increase in APOBEC3A until 24 hours but no increase in APOBEC3G (inset; n = 3).

    Techniques Used: Inhibition

    Blocking IL-27 signaling reverses inhibition of HIV infection . (A) Inhibition of IL-27 signal transduction by blocking JAK activation interrupts STAT1 and STAT3 phosphorylation. Macrophages were treated with JAK inhibitor at 1 or 10 nM for 1 hour before
    Figure Legend Snippet: Blocking IL-27 signaling reverses inhibition of HIV infection . (A) Inhibition of IL-27 signal transduction by blocking JAK activation interrupts STAT1 and STAT3 phosphorylation. Macrophages were treated with JAK inhibitor at 1 or 10 nM for 1 hour before

    Techniques Used: Blocking Assay, Inhibition, Infection, Transduction, Activation Assay

    4) Product Images from "Interleukin-27 inhibition of HIV-1 involves an intermediate induction of type I interferon"

    Article Title: Interleukin-27 inhibition of HIV-1 involves an intermediate induction of type I interferon

    Journal:

    doi: 10.1182/blood-2009-03-211540

    IL-27 inhibits HIV infection in macrophages . (A) Monocyte-derived macrophages were infected with 10 3 TCID 50 /mL BaL for 2 hours. Cells were washed, 10% DMEM added, and IL-27 at 0, 1, 10, and 100 ng/mL added only once after infection. Cells were fed every
    Figure Legend Snippet: IL-27 inhibits HIV infection in macrophages . (A) Monocyte-derived macrophages were infected with 10 3 TCID 50 /mL BaL for 2 hours. Cells were washed, 10% DMEM added, and IL-27 at 0, 1, 10, and 100 ng/mL added only once after infection. Cells were fed every

    Techniques Used: Infection, Derivative Assay

    IFN-α up-regulates IL-27 and IL-27 receptor mRNA expression . (A) Monocyte-derived macrophages (n = 3) were stimulated with IFN-α (10 ng/mL, 4 hours) in parallel with mock-treated cultures, and RNA was extracted and processed for microarray
    Figure Legend Snippet: IFN-α up-regulates IL-27 and IL-27 receptor mRNA expression . (A) Monocyte-derived macrophages (n = 3) were stimulated with IFN-α (10 ng/mL, 4 hours) in parallel with mock-treated cultures, and RNA was extracted and processed for microarray

    Techniques Used: Expressing, Derivative Assay, Microarray

    IL-27 induction of IFN-α and IFN-β supports APOBEC3A/G expression and inhibition of HIV . (A) PBMC were treated with IL-27 at 100 ng/mL for 30 minutes to 24 hours. RNA was reverse transcribed to cDNA, and amplification was performed using
    Figure Legend Snippet: IL-27 induction of IFN-α and IFN-β supports APOBEC3A/G expression and inhibition of HIV . (A) PBMC were treated with IL-27 at 100 ng/mL for 30 minutes to 24 hours. RNA was reverse transcribed to cDNA, and amplification was performed using

    Techniques Used: Expressing, Inhibition, Amplification

    IL-27 enhances macrophage APOBEC RNA expression . Monocyte-derived macrophages were treated 18 to 24 hours with IL-27 at 100 ng/mL or IFN-α at 10 ng/mL. Cells were lysed and processed for RNA following QIAGEN's RNeasy protocol, and gene transcription
    Figure Legend Snippet: IL-27 enhances macrophage APOBEC RNA expression . Monocyte-derived macrophages were treated 18 to 24 hours with IL-27 at 100 ng/mL or IFN-α at 10 ng/mL. Cells were lysed and processed for RNA following QIAGEN's RNeasy protocol, and gene transcription

    Techniques Used: RNA Expression, Derivative Assay

    IL-27 inhibition of T-cell HIV includes an IFN intermediate . (A) IL-27 induction of IFN- α in T cells at 0.5 to 4 hours (4 hours shown; n = 2), without corresponding increase in APOBEC3A until 24 hours but no increase in APOBEC3G (inset; n = 3).
    Figure Legend Snippet: IL-27 inhibition of T-cell HIV includes an IFN intermediate . (A) IL-27 induction of IFN- α in T cells at 0.5 to 4 hours (4 hours shown; n = 2), without corresponding increase in APOBEC3A until 24 hours but no increase in APOBEC3G (inset; n = 3).

    Techniques Used: Inhibition

    Blocking IL-27 signaling reverses inhibition of HIV infection . (A) Inhibition of IL-27 signal transduction by blocking JAK activation interrupts STAT1 and STAT3 phosphorylation. Macrophages were treated with JAK inhibitor at 1 or 10 nM for 1 hour before
    Figure Legend Snippet: Blocking IL-27 signaling reverses inhibition of HIV infection . (A) Inhibition of IL-27 signal transduction by blocking JAK activation interrupts STAT1 and STAT3 phosphorylation. Macrophages were treated with JAK inhibitor at 1 or 10 nM for 1 hour before

    Techniques Used: Blocking Assay, Inhibition, Infection, Transduction, Activation Assay

    5) Product Images from "Activation of naïve CD4+ T cells re-tunes STAT1 signaling to deliver unique cytokine responses in memory CD4+ T cells"

    Article Title: Activation of naïve CD4+ T cells re-tunes STAT1 signaling to deliver unique cytokine responses in memory CD4+ T cells

    Journal: Nature immunology

    doi: 10.1038/s41590-019-0350-0

    CD4 + T cell subsets show different response to IL-6. (a) Representative flow cytometry analysis of STAT1 and STAT3 responses in naïve (T N ), central memory (T CM ), effector (T Eff ) and effector memory (T EM ) CD4 + T cells after 30 min IL-6 stimulation (20ng/ml). Numbers indicate the percentage of pY-STAT1 or pY STAT3 staining. Temporal changes in pY-STAT1 and pY-STAT3 are shown for each T cell subset following IL 6 stimulation (n=3). (b) Detection of pY-STAT1 and pY-STAT3 in CD4 + T N , CD4 + T CM , CD4 + T Eff and CD4 + T EM cells from WT and IL6ra -/- mice. CD4 + T cells were stimulated for 30 min with an equimolar concentration of IL-6 or an IL-6-sIL-6R fusion protein (HDS) (n=3). (c) Intracellular flow cytometry analysis of pY-STAT1 in CD4 + T cells following 30 min stimulation with IL-6, IL-27 or IFNγ (20ng/ml) (n=3). (d) Microarray expression data is presented for CD4 + T N (n=3), CD4 + T EM (n=3), and in vitro expanded CD4 + effector-like T cells (See Supplementary Fig.2a , CD4 + T EXP ) (n=4) treated with 20ng/ml IL-6 for 6 hours. Analysis was confined to genes displaying both a relative signal intensity of > 150 and > 1.5-fold alteration in expression following IL-6 treatment ( P
    Figure Legend Snippet: CD4 + T cell subsets show different response to IL-6. (a) Representative flow cytometry analysis of STAT1 and STAT3 responses in naïve (T N ), central memory (T CM ), effector (T Eff ) and effector memory (T EM ) CD4 + T cells after 30 min IL-6 stimulation (20ng/ml). Numbers indicate the percentage of pY-STAT1 or pY STAT3 staining. Temporal changes in pY-STAT1 and pY-STAT3 are shown for each T cell subset following IL 6 stimulation (n=3). (b) Detection of pY-STAT1 and pY-STAT3 in CD4 + T N , CD4 + T CM , CD4 + T Eff and CD4 + T EM cells from WT and IL6ra -/- mice. CD4 + T cells were stimulated for 30 min with an equimolar concentration of IL-6 or an IL-6-sIL-6R fusion protein (HDS) (n=3). (c) Intracellular flow cytometry analysis of pY-STAT1 in CD4 + T cells following 30 min stimulation with IL-6, IL-27 or IFNγ (20ng/ml) (n=3). (d) Microarray expression data is presented for CD4 + T N (n=3), CD4 + T EM (n=3), and in vitro expanded CD4 + effector-like T cells (See Supplementary Fig.2a , CD4 + T EXP ) (n=4) treated with 20ng/ml IL-6 for 6 hours. Analysis was confined to genes displaying both a relative signal intensity of > 150 and > 1.5-fold alteration in expression following IL-6 treatment ( P

    Techniques Used: Flow Cytometry, Staining, Mouse Assay, Concentration Assay, Microarray, Expressing, In Vitro

    6) Product Images from "Interleukin-27 inhibition of HIV-1 involves an intermediate induction of type I interferon"

    Article Title: Interleukin-27 inhibition of HIV-1 involves an intermediate induction of type I interferon

    Journal:

    doi: 10.1182/blood-2009-03-211540

    IL-27 inhibits HIV infection in macrophages . (A) Monocyte-derived macrophages were infected with 10 3 TCID 50 /mL BaL for 2 hours. Cells were washed, 10% DMEM added, and IL-27 at 0, 1, 10, and 100 ng/mL added only once after infection. Cells were fed every
    Figure Legend Snippet: IL-27 inhibits HIV infection in macrophages . (A) Monocyte-derived macrophages were infected with 10 3 TCID 50 /mL BaL for 2 hours. Cells were washed, 10% DMEM added, and IL-27 at 0, 1, 10, and 100 ng/mL added only once after infection. Cells were fed every

    Techniques Used: Infection, Derivative Assay

    IFN-α up-regulates IL-27 and IL-27 receptor mRNA expression . (A) Monocyte-derived macrophages (n = 3) were stimulated with IFN-α (10 ng/mL, 4 hours) in parallel with mock-treated cultures, and RNA was extracted and processed for microarray
    Figure Legend Snippet: IFN-α up-regulates IL-27 and IL-27 receptor mRNA expression . (A) Monocyte-derived macrophages (n = 3) were stimulated with IFN-α (10 ng/mL, 4 hours) in parallel with mock-treated cultures, and RNA was extracted and processed for microarray

    Techniques Used: Expressing, Derivative Assay, Microarray

    IL-27 induction of IFN-α and IFN-β supports APOBEC3A/G expression and inhibition of HIV . (A) PBMC were treated with IL-27 at 100 ng/mL for 30 minutes to 24 hours. RNA was reverse transcribed to cDNA, and amplification was performed using
    Figure Legend Snippet: IL-27 induction of IFN-α and IFN-β supports APOBEC3A/G expression and inhibition of HIV . (A) PBMC were treated with IL-27 at 100 ng/mL for 30 minutes to 24 hours. RNA was reverse transcribed to cDNA, and amplification was performed using

    Techniques Used: Expressing, Inhibition, Amplification

    IL-27 enhances macrophage APOBEC RNA expression . Monocyte-derived macrophages were treated 18 to 24 hours with IL-27 at 100 ng/mL or IFN-α at 10 ng/mL. Cells were lysed and processed for RNA following QIAGEN's RNeasy protocol, and gene transcription
    Figure Legend Snippet: IL-27 enhances macrophage APOBEC RNA expression . Monocyte-derived macrophages were treated 18 to 24 hours with IL-27 at 100 ng/mL or IFN-α at 10 ng/mL. Cells were lysed and processed for RNA following QIAGEN's RNeasy protocol, and gene transcription

    Techniques Used: RNA Expression, Derivative Assay

    IL-27 inhibition of T-cell HIV includes an IFN intermediate . (A) IL-27 induction of IFN- α in T cells at 0.5 to 4 hours (4 hours shown; n = 2), without corresponding increase in APOBEC3A until 24 hours but no increase in APOBEC3G (inset; n = 3).
    Figure Legend Snippet: IL-27 inhibition of T-cell HIV includes an IFN intermediate . (A) IL-27 induction of IFN- α in T cells at 0.5 to 4 hours (4 hours shown; n = 2), without corresponding increase in APOBEC3A until 24 hours but no increase in APOBEC3G (inset; n = 3).

    Techniques Used: Inhibition

    Blocking IL-27 signaling reverses inhibition of HIV infection . (A) Inhibition of IL-27 signal transduction by blocking JAK activation interrupts STAT1 and STAT3 phosphorylation. Macrophages were treated with JAK inhibitor at 1 or 10 nM for 1 hour before
    Figure Legend Snippet: Blocking IL-27 signaling reverses inhibition of HIV infection . (A) Inhibition of IL-27 signal transduction by blocking JAK activation interrupts STAT1 and STAT3 phosphorylation. Macrophages were treated with JAK inhibitor at 1 or 10 nM for 1 hour before

    Techniques Used: Blocking Assay, Inhibition, Infection, Transduction, Activation Assay

    7) Product Images from "Interleukin-27 inhibition of HIV-1 involves an intermediate induction of type I interferon"

    Article Title: Interleukin-27 inhibition of HIV-1 involves an intermediate induction of type I interferon

    Journal:

    doi: 10.1182/blood-2009-03-211540

    IL-27 inhibits HIV infection in macrophages . (A) Monocyte-derived macrophages were infected with 10 3 TCID 50 /mL BaL for 2 hours. Cells were washed, 10% DMEM added, and IL-27 at 0, 1, 10, and 100 ng/mL added only once after infection. Cells were fed every
    Figure Legend Snippet: IL-27 inhibits HIV infection in macrophages . (A) Monocyte-derived macrophages were infected with 10 3 TCID 50 /mL BaL for 2 hours. Cells were washed, 10% DMEM added, and IL-27 at 0, 1, 10, and 100 ng/mL added only once after infection. Cells were fed every

    Techniques Used: Infection, Derivative Assay

    IFN-α up-regulates IL-27 and IL-27 receptor mRNA expression . (A) Monocyte-derived macrophages (n = 3) were stimulated with IFN-α (10 ng/mL, 4 hours) in parallel with mock-treated cultures, and RNA was extracted and processed for microarray
    Figure Legend Snippet: IFN-α up-regulates IL-27 and IL-27 receptor mRNA expression . (A) Monocyte-derived macrophages (n = 3) were stimulated with IFN-α (10 ng/mL, 4 hours) in parallel with mock-treated cultures, and RNA was extracted and processed for microarray

    Techniques Used: Expressing, Derivative Assay, Microarray

    IL-27 induction of IFN-α and IFN-β supports APOBEC3A/G expression and inhibition of HIV . (A) PBMC were treated with IL-27 at 100 ng/mL for 30 minutes to 24 hours. RNA was reverse transcribed to cDNA, and amplification was performed using
    Figure Legend Snippet: IL-27 induction of IFN-α and IFN-β supports APOBEC3A/G expression and inhibition of HIV . (A) PBMC were treated with IL-27 at 100 ng/mL for 30 minutes to 24 hours. RNA was reverse transcribed to cDNA, and amplification was performed using

    Techniques Used: Expressing, Inhibition, Amplification

    IL-27 enhances macrophage APOBEC RNA expression . Monocyte-derived macrophages were treated 18 to 24 hours with IL-27 at 100 ng/mL or IFN-α at 10 ng/mL. Cells were lysed and processed for RNA following QIAGEN's RNeasy protocol, and gene transcription
    Figure Legend Snippet: IL-27 enhances macrophage APOBEC RNA expression . Monocyte-derived macrophages were treated 18 to 24 hours with IL-27 at 100 ng/mL or IFN-α at 10 ng/mL. Cells were lysed and processed for RNA following QIAGEN's RNeasy protocol, and gene transcription

    Techniques Used: RNA Expression, Derivative Assay

    IL-27 inhibition of T-cell HIV includes an IFN intermediate . (A) IL-27 induction of IFN- α in T cells at 0.5 to 4 hours (4 hours shown; n = 2), without corresponding increase in APOBEC3A until 24 hours but no increase in APOBEC3G (inset; n = 3).
    Figure Legend Snippet: IL-27 inhibition of T-cell HIV includes an IFN intermediate . (A) IL-27 induction of IFN- α in T cells at 0.5 to 4 hours (4 hours shown; n = 2), without corresponding increase in APOBEC3A until 24 hours but no increase in APOBEC3G (inset; n = 3).

    Techniques Used: Inhibition

    Blocking IL-27 signaling reverses inhibition of HIV infection . (A) Inhibition of IL-27 signal transduction by blocking JAK activation interrupts STAT1 and STAT3 phosphorylation. Macrophages were treated with JAK inhibitor at 1 or 10 nM for 1 hour before
    Figure Legend Snippet: Blocking IL-27 signaling reverses inhibition of HIV infection . (A) Inhibition of IL-27 signal transduction by blocking JAK activation interrupts STAT1 and STAT3 phosphorylation. Macrophages were treated with JAK inhibitor at 1 or 10 nM for 1 hour before

    Techniques Used: Blocking Assay, Inhibition, Infection, Transduction, Activation Assay

    8) Product Images from "Interleukin-27 inhibition of HIV-1 involves an intermediate induction of type I interferon"

    Article Title: Interleukin-27 inhibition of HIV-1 involves an intermediate induction of type I interferon

    Journal:

    doi: 10.1182/blood-2009-03-211540

    IL-27 inhibits HIV infection in macrophages . (A) Monocyte-derived macrophages were infected with 10 3 TCID 50 /mL BaL for 2 hours. Cells were washed, 10% DMEM added, and IL-27 at 0, 1, 10, and 100 ng/mL added only once after infection. Cells were fed every
    Figure Legend Snippet: IL-27 inhibits HIV infection in macrophages . (A) Monocyte-derived macrophages were infected with 10 3 TCID 50 /mL BaL for 2 hours. Cells were washed, 10% DMEM added, and IL-27 at 0, 1, 10, and 100 ng/mL added only once after infection. Cells were fed every

    Techniques Used: Infection, Derivative Assay

    IFN-α up-regulates IL-27 and IL-27 receptor mRNA expression . (A) Monocyte-derived macrophages (n = 3) were stimulated with IFN-α (10 ng/mL, 4 hours) in parallel with mock-treated cultures, and RNA was extracted and processed for microarray
    Figure Legend Snippet: IFN-α up-regulates IL-27 and IL-27 receptor mRNA expression . (A) Monocyte-derived macrophages (n = 3) were stimulated with IFN-α (10 ng/mL, 4 hours) in parallel with mock-treated cultures, and RNA was extracted and processed for microarray

    Techniques Used: Expressing, Derivative Assay, Microarray

    IL-27 induction of IFN-α and IFN-β supports APOBEC3A/G expression and inhibition of HIV . (A) PBMC were treated with IL-27 at 100 ng/mL for 30 minutes to 24 hours. RNA was reverse transcribed to cDNA, and amplification was performed using
    Figure Legend Snippet: IL-27 induction of IFN-α and IFN-β supports APOBEC3A/G expression and inhibition of HIV . (A) PBMC were treated with IL-27 at 100 ng/mL for 30 minutes to 24 hours. RNA was reverse transcribed to cDNA, and amplification was performed using

    Techniques Used: Expressing, Inhibition, Amplification

    IL-27 enhances macrophage APOBEC RNA expression . Monocyte-derived macrophages were treated 18 to 24 hours with IL-27 at 100 ng/mL or IFN-α at 10 ng/mL. Cells were lysed and processed for RNA following QIAGEN's RNeasy protocol, and gene transcription
    Figure Legend Snippet: IL-27 enhances macrophage APOBEC RNA expression . Monocyte-derived macrophages were treated 18 to 24 hours with IL-27 at 100 ng/mL or IFN-α at 10 ng/mL. Cells were lysed and processed for RNA following QIAGEN's RNeasy protocol, and gene transcription

    Techniques Used: RNA Expression, Derivative Assay

    IL-27 inhibition of T-cell HIV includes an IFN intermediate . (A) IL-27 induction of IFN- α in T cells at 0.5 to 4 hours (4 hours shown; n = 2), without corresponding increase in APOBEC3A until 24 hours but no increase in APOBEC3G (inset; n = 3).
    Figure Legend Snippet: IL-27 inhibition of T-cell HIV includes an IFN intermediate . (A) IL-27 induction of IFN- α in T cells at 0.5 to 4 hours (4 hours shown; n = 2), without corresponding increase in APOBEC3A until 24 hours but no increase in APOBEC3G (inset; n = 3).

    Techniques Used: Inhibition

    Blocking IL-27 signaling reverses inhibition of HIV infection . (A) Inhibition of IL-27 signal transduction by blocking JAK activation interrupts STAT1 and STAT3 phosphorylation. Macrophages were treated with JAK inhibitor at 1 or 10 nM for 1 hour before
    Figure Legend Snippet: Blocking IL-27 signaling reverses inhibition of HIV infection . (A) Inhibition of IL-27 signal transduction by blocking JAK activation interrupts STAT1 and STAT3 phosphorylation. Macrophages were treated with JAK inhibitor at 1 or 10 nM for 1 hour before

    Techniques Used: Blocking Assay, Inhibition, Infection, Transduction, Activation Assay

    9) Product Images from "Interleukin-27 inhibition of HIV-1 involves an intermediate induction of type I interferon"

    Article Title: Interleukin-27 inhibition of HIV-1 involves an intermediate induction of type I interferon

    Journal:

    doi: 10.1182/blood-2009-03-211540

    IL-27 inhibits HIV infection in macrophages . (A) Monocyte-derived macrophages were infected with 10 3 TCID 50 /mL BaL for 2 hours. Cells were washed, 10% DMEM added, and IL-27 at 0, 1, 10, and 100 ng/mL added only once after infection. Cells were fed every
    Figure Legend Snippet: IL-27 inhibits HIV infection in macrophages . (A) Monocyte-derived macrophages were infected with 10 3 TCID 50 /mL BaL for 2 hours. Cells were washed, 10% DMEM added, and IL-27 at 0, 1, 10, and 100 ng/mL added only once after infection. Cells were fed every

    Techniques Used: Infection, Derivative Assay

    IFN-α up-regulates IL-27 and IL-27 receptor mRNA expression . (A) Monocyte-derived macrophages (n = 3) were stimulated with IFN-α (10 ng/mL, 4 hours) in parallel with mock-treated cultures, and RNA was extracted and processed for microarray
    Figure Legend Snippet: IFN-α up-regulates IL-27 and IL-27 receptor mRNA expression . (A) Monocyte-derived macrophages (n = 3) were stimulated with IFN-α (10 ng/mL, 4 hours) in parallel with mock-treated cultures, and RNA was extracted and processed for microarray

    Techniques Used: Expressing, Derivative Assay, Microarray

    IL-27 induction of IFN-α and IFN-β supports APOBEC3A/G expression and inhibition of HIV . (A) PBMC were treated with IL-27 at 100 ng/mL for 30 minutes to 24 hours. RNA was reverse transcribed to cDNA, and amplification was performed using
    Figure Legend Snippet: IL-27 induction of IFN-α and IFN-β supports APOBEC3A/G expression and inhibition of HIV . (A) PBMC were treated with IL-27 at 100 ng/mL for 30 minutes to 24 hours. RNA was reverse transcribed to cDNA, and amplification was performed using

    Techniques Used: Expressing, Inhibition, Amplification

    IL-27 enhances macrophage APOBEC RNA expression . Monocyte-derived macrophages were treated 18 to 24 hours with IL-27 at 100 ng/mL or IFN-α at 10 ng/mL. Cells were lysed and processed for RNA following QIAGEN's RNeasy protocol, and gene transcription
    Figure Legend Snippet: IL-27 enhances macrophage APOBEC RNA expression . Monocyte-derived macrophages were treated 18 to 24 hours with IL-27 at 100 ng/mL or IFN-α at 10 ng/mL. Cells were lysed and processed for RNA following QIAGEN's RNeasy protocol, and gene transcription

    Techniques Used: RNA Expression, Derivative Assay

    IL-27 inhibition of T-cell HIV includes an IFN intermediate . (A) IL-27 induction of IFN- α in T cells at 0.5 to 4 hours (4 hours shown; n = 2), without corresponding increase in APOBEC3A until 24 hours but no increase in APOBEC3G (inset; n = 3).
    Figure Legend Snippet: IL-27 inhibition of T-cell HIV includes an IFN intermediate . (A) IL-27 induction of IFN- α in T cells at 0.5 to 4 hours (4 hours shown; n = 2), without corresponding increase in APOBEC3A until 24 hours but no increase in APOBEC3G (inset; n = 3).

    Techniques Used: Inhibition

    Blocking IL-27 signaling reverses inhibition of HIV infection . (A) Inhibition of IL-27 signal transduction by blocking JAK activation interrupts STAT1 and STAT3 phosphorylation. Macrophages were treated with JAK inhibitor at 1 or 10 nM for 1 hour before
    Figure Legend Snippet: Blocking IL-27 signaling reverses inhibition of HIV infection . (A) Inhibition of IL-27 signal transduction by blocking JAK activation interrupts STAT1 and STAT3 phosphorylation. Macrophages were treated with JAK inhibitor at 1 or 10 nM for 1 hour before

    Techniques Used: Blocking Assay, Inhibition, Infection, Transduction, Activation Assay

    10) Product Images from "Immunobiotic Lactobacillus jensenii TL2937 Alleviates Dextran Sodium Sulfate-Induced Colitis by Differentially Modulating the Transcriptomic Response of Intestinal Epithelial Cells"

    Article Title: Immunobiotic Lactobacillus jensenii TL2937 Alleviates Dextran Sodium Sulfate-Induced Colitis by Differentially Modulating the Transcriptomic Response of Intestinal Epithelial Cells

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2020.02174

    Effect of immunobiotic Lactobacillus jensenii TL2937 on the inflammatory response induced in mice by dextran sodium sulfate (DSS) administration. L. jensenii TL2937 was orally administered to different groups of mice (10 8 cells/mouse/day) before (PRE DSS+TL2937 group) the administration of DSS for 7 days. Untreated mice challenged only with DSS were used as controls. The animals were sacrificed at different time points: the end of immunobiotic administration (day −1), the start of DSS administration (day 1), the end of DSS administration (day 7), and 7 days after the last DSS administration (day 14). (A) Colon inflammatory cytokines and chemokines (IL-1β, IL-6, CXCL1, IL-15, MCP-1), (B) colon IFN-γ and IL-17, and (C) colon regulatory cytokines (TGF-β, IL-10, IL-27) were evaluated at the indicated days. The results represent three independent experiments. Significant differences when compared to the DSS control group: * P
    Figure Legend Snippet: Effect of immunobiotic Lactobacillus jensenii TL2937 on the inflammatory response induced in mice by dextran sodium sulfate (DSS) administration. L. jensenii TL2937 was orally administered to different groups of mice (10 8 cells/mouse/day) before (PRE DSS+TL2937 group) the administration of DSS for 7 days. Untreated mice challenged only with DSS were used as controls. The animals were sacrificed at different time points: the end of immunobiotic administration (day −1), the start of DSS administration (day 1), the end of DSS administration (day 7), and 7 days after the last DSS administration (day 14). (A) Colon inflammatory cytokines and chemokines (IL-1β, IL-6, CXCL1, IL-15, MCP-1), (B) colon IFN-γ and IL-17, and (C) colon regulatory cytokines (TGF-β, IL-10, IL-27) were evaluated at the indicated days. The results represent three independent experiments. Significant differences when compared to the DSS control group: * P

    Techniques Used: Mouse Assay

    11) Product Images from "Murine myeloid-derived suppressor cells are a source of elevated levels of interleukin-27 in early life and compromise control of bacterial infection"

    Article Title: Murine myeloid-derived suppressor cells are a source of elevated levels of interleukin-27 in early life and compromise control of bacterial infection

    Journal: Immunology and cell biology

    doi: 10.1111/imcb.12224

    Circulating IL-27 levels are elevated in neonates. ( a ) Blood was collected from mice in the indicated age groups and serum levels of IL-27 were measured by luminescent immunoassay. Each symbol represents an individual animal finding. Statistical significance was determined using unpaired t tests with Welch’s correction.
    Figure Legend Snippet: Circulating IL-27 levels are elevated in neonates. ( a ) Blood was collected from mice in the indicated age groups and serum levels of IL-27 were measured by luminescent immunoassay. Each symbol represents an individual animal finding. Statistical significance was determined using unpaired t tests with Welch’s correction.

    Techniques Used: Mouse Assay

    Isolated MDSCs produce IL-27. MDSCs were isolated from neonatal C57BL/6 mouse spleens as described in the Methods . ( a ) The isolated cells were labeled for surface-expressed Gr-1 and intracellular IL-27 and analyzed by flow cytometry. A representative dot plot relative to isotype controls is shown. ( b ) MDSC expression of the indicated genes relative to a no template control was determined by real time PCR. Six independent measurements using MDSCs obtained from pups ranging 5–8 days of age are combined and shown. ( c ) IL-27 concentration was determined in 24 h MDSC culture supernatants by ELISA. Seven independent measurements using MDSCs obtained from pups ranging 5–8 days of age are combined and shown. ( d ) The nitrite concentration in 48 h MDSC culture supernatants was measured. Five independent measurements using MDSCs obtained from pups ranging 5–10 days of age are combined and shown. ( e ) Arginase activity was measured relative to purified standards. Eight independent measurements using MDSCs obtained from pups ranging 5–8 days of age are combined and shown. ( f ) Oxidative burst was measured in MDSCs using Dihydrorhodamine 123. Data using MDSCs obtained from six day old pups representative of three separate measurements is shown.
    Figure Legend Snippet: Isolated MDSCs produce IL-27. MDSCs were isolated from neonatal C57BL/6 mouse spleens as described in the Methods . ( a ) The isolated cells were labeled for surface-expressed Gr-1 and intracellular IL-27 and analyzed by flow cytometry. A representative dot plot relative to isotype controls is shown. ( b ) MDSC expression of the indicated genes relative to a no template control was determined by real time PCR. Six independent measurements using MDSCs obtained from pups ranging 5–8 days of age are combined and shown. ( c ) IL-27 concentration was determined in 24 h MDSC culture supernatants by ELISA. Seven independent measurements using MDSCs obtained from pups ranging 5–8 days of age are combined and shown. ( d ) The nitrite concentration in 48 h MDSC culture supernatants was measured. Five independent measurements using MDSCs obtained from pups ranging 5–10 days of age are combined and shown. ( e ) Arginase activity was measured relative to purified standards. Eight independent measurements using MDSCs obtained from pups ranging 5–8 days of age are combined and shown. ( f ) Oxidative burst was measured in MDSCs using Dihydrorhodamine 123. Data using MDSCs obtained from six day old pups representative of three separate measurements is shown.

    Techniques Used: Isolation, Labeling, Flow Cytometry, Expressing, Real-time Polymerase Chain Reaction, Concentration Assay, Enzyme-linked Immunosorbent Assay, Activity Assay, Purification

    Gr-1 + cells that produce IL-27 are more abundant in neonates. Splenocytes from C57BL/6 mice at the indicated age were labeled for surface-expressed Gr-1 and intracellular IL-27 and analyzed by flow cytometry. ( a ) Representative dot plots relative to isotype controls are shown. ( b ) The combined percent Gr-1 + IL-27 + population from at least 8 mice at each age group are shown. Statistical significance was determined using an ANOVA and Tukey’s multiple comparison’s test.
    Figure Legend Snippet: Gr-1 + cells that produce IL-27 are more abundant in neonates. Splenocytes from C57BL/6 mice at the indicated age were labeled for surface-expressed Gr-1 and intracellular IL-27 and analyzed by flow cytometry. ( a ) Representative dot plots relative to isotype controls are shown. ( b ) The combined percent Gr-1 + IL-27 + population from at least 8 mice at each age group are shown. Statistical significance was determined using an ANOVA and Tukey’s multiple comparison’s test.

    Techniques Used: Mouse Assay, Labeling, Flow Cytometry

    MDSCs compromise macrophage control of E. coli . Bone marrow-derived macrophages were infected with Escherichia coli at an MOI of 20 in the presence or absence of MDSCs (5–13 days of age) at the indicated ratio. Recovery of intracellular bacteria was enumerated at 18 h and shown as mean log 10 recovered colony forming units ± standard error ( a ). Statistical significance in a combined six experiments was determined using a paired samples t test . ( b ) MDSCs were added to cultures where shown at the indicated ratio to macrophages. A combined four experiments are shown. Statistical significance was determined using paired samples t tests . ( c ) Macrophages were cultured as indicated ± MDSCs (ratio of 1:1 with macrophages) in the presence or absence of a soluble receptor to neutralize IL-27 (sIL27R). Five combined experiments are shown. Statistical significance was determined using paired samples t tests . ( d ) The concentration of TNF-α in culture supernatants was determined by ELISA. The presence of bacteria (Ec), sIL-27R, and ratio of MDSCs to macrophages (0.1X or 1X) is indicated. Results from an individual experiment representative of multiple are shown.
    Figure Legend Snippet: MDSCs compromise macrophage control of E. coli . Bone marrow-derived macrophages were infected with Escherichia coli at an MOI of 20 in the presence or absence of MDSCs (5–13 days of age) at the indicated ratio. Recovery of intracellular bacteria was enumerated at 18 h and shown as mean log 10 recovered colony forming units ± standard error ( a ). Statistical significance in a combined six experiments was determined using a paired samples t test . ( b ) MDSCs were added to cultures where shown at the indicated ratio to macrophages. A combined four experiments are shown. Statistical significance was determined using paired samples t tests . ( c ) Macrophages were cultured as indicated ± MDSCs (ratio of 1:1 with macrophages) in the presence or absence of a soluble receptor to neutralize IL-27 (sIL27R). Five combined experiments are shown. Statistical significance was determined using paired samples t tests . ( d ) The concentration of TNF-α in culture supernatants was determined by ELISA. The presence of bacteria (Ec), sIL-27R, and ratio of MDSCs to macrophages (0.1X or 1X) is indicated. Results from an individual experiment representative of multiple are shown.

    Techniques Used: Derivative Assay, Infection, Cell Culture, Concentration Assay, Enzyme-linked Immunosorbent Assay

    12) Product Images from "Interleukin-27 inhibition of HIV-1 involves an intermediate induction of type I interferon"

    Article Title: Interleukin-27 inhibition of HIV-1 involves an intermediate induction of type I interferon

    Journal:

    doi: 10.1182/blood-2009-03-211540

    IL-27 inhibits HIV infection in macrophages . (A) Monocyte-derived macrophages were infected with 10 3 TCID 50 /mL BaL for 2 hours. Cells were washed, 10% DMEM added, and IL-27 at 0, 1, 10, and 100 ng/mL added only once after infection. Cells were fed every
    Figure Legend Snippet: IL-27 inhibits HIV infection in macrophages . (A) Monocyte-derived macrophages were infected with 10 3 TCID 50 /mL BaL for 2 hours. Cells were washed, 10% DMEM added, and IL-27 at 0, 1, 10, and 100 ng/mL added only once after infection. Cells were fed every

    Techniques Used: Infection, Derivative Assay

    IFN-α up-regulates IL-27 and IL-27 receptor mRNA expression . (A) Monocyte-derived macrophages (n = 3) were stimulated with IFN-α (10 ng/mL, 4 hours) in parallel with mock-treated cultures, and RNA was extracted and processed for microarray
    Figure Legend Snippet: IFN-α up-regulates IL-27 and IL-27 receptor mRNA expression . (A) Monocyte-derived macrophages (n = 3) were stimulated with IFN-α (10 ng/mL, 4 hours) in parallel with mock-treated cultures, and RNA was extracted and processed for microarray

    Techniques Used: Expressing, Derivative Assay, Microarray

    IL-27 induction of IFN-α and IFN-β supports APOBEC3A/G expression and inhibition of HIV . (A) PBMC were treated with IL-27 at 100 ng/mL for 30 minutes to 24 hours. RNA was reverse transcribed to cDNA, and amplification was performed using
    Figure Legend Snippet: IL-27 induction of IFN-α and IFN-β supports APOBEC3A/G expression and inhibition of HIV . (A) PBMC were treated with IL-27 at 100 ng/mL for 30 minutes to 24 hours. RNA was reverse transcribed to cDNA, and amplification was performed using

    Techniques Used: Expressing, Inhibition, Amplification

    IL-27 enhances macrophage APOBEC RNA expression . Monocyte-derived macrophages were treated 18 to 24 hours with IL-27 at 100 ng/mL or IFN-α at 10 ng/mL. Cells were lysed and processed for RNA following QIAGEN's RNeasy protocol, and gene transcription
    Figure Legend Snippet: IL-27 enhances macrophage APOBEC RNA expression . Monocyte-derived macrophages were treated 18 to 24 hours with IL-27 at 100 ng/mL or IFN-α at 10 ng/mL. Cells were lysed and processed for RNA following QIAGEN's RNeasy protocol, and gene transcription

    Techniques Used: RNA Expression, Derivative Assay

    IL-27 inhibition of T-cell HIV includes an IFN intermediate . (A) IL-27 induction of IFN- α in T cells at 0.5 to 4 hours (4 hours shown; n = 2), without corresponding increase in APOBEC3A until 24 hours but no increase in APOBEC3G (inset; n = 3).
    Figure Legend Snippet: IL-27 inhibition of T-cell HIV includes an IFN intermediate . (A) IL-27 induction of IFN- α in T cells at 0.5 to 4 hours (4 hours shown; n = 2), without corresponding increase in APOBEC3A until 24 hours but no increase in APOBEC3G (inset; n = 3).

    Techniques Used: Inhibition

    Blocking IL-27 signaling reverses inhibition of HIV infection . (A) Inhibition of IL-27 signal transduction by blocking JAK activation interrupts STAT1 and STAT3 phosphorylation. Macrophages were treated with JAK inhibitor at 1 or 10 nM for 1 hour before
    Figure Legend Snippet: Blocking IL-27 signaling reverses inhibition of HIV infection . (A) Inhibition of IL-27 signal transduction by blocking JAK activation interrupts STAT1 and STAT3 phosphorylation. Macrophages were treated with JAK inhibitor at 1 or 10 nM for 1 hour before

    Techniques Used: Blocking Assay, Inhibition, Infection, Transduction, Activation Assay

    13) Product Images from "Interleukin-27 inhibition of HIV-1 involves an intermediate induction of type I interferon"

    Article Title: Interleukin-27 inhibition of HIV-1 involves an intermediate induction of type I interferon

    Journal:

    doi: 10.1182/blood-2009-03-211540

    IL-27 inhibits HIV infection in macrophages . (A) Monocyte-derived macrophages were infected with 10 3 TCID 50 /mL BaL for 2 hours. Cells were washed, 10% DMEM added, and IL-27 at 0, 1, 10, and 100 ng/mL added only once after infection. Cells were fed every
    Figure Legend Snippet: IL-27 inhibits HIV infection in macrophages . (A) Monocyte-derived macrophages were infected with 10 3 TCID 50 /mL BaL for 2 hours. Cells were washed, 10% DMEM added, and IL-27 at 0, 1, 10, and 100 ng/mL added only once after infection. Cells were fed every

    Techniques Used: Infection, Derivative Assay

    IFN-α up-regulates IL-27 and IL-27 receptor mRNA expression . (A) Monocyte-derived macrophages (n = 3) were stimulated with IFN-α (10 ng/mL, 4 hours) in parallel with mock-treated cultures, and RNA was extracted and processed for microarray
    Figure Legend Snippet: IFN-α up-regulates IL-27 and IL-27 receptor mRNA expression . (A) Monocyte-derived macrophages (n = 3) were stimulated with IFN-α (10 ng/mL, 4 hours) in parallel with mock-treated cultures, and RNA was extracted and processed for microarray

    Techniques Used: Expressing, Derivative Assay, Microarray

    IL-27 induction of IFN-α and IFN-β supports APOBEC3A/G expression and inhibition of HIV . (A) PBMC were treated with IL-27 at 100 ng/mL for 30 minutes to 24 hours. RNA was reverse transcribed to cDNA, and amplification was performed using
    Figure Legend Snippet: IL-27 induction of IFN-α and IFN-β supports APOBEC3A/G expression and inhibition of HIV . (A) PBMC were treated with IL-27 at 100 ng/mL for 30 minutes to 24 hours. RNA was reverse transcribed to cDNA, and amplification was performed using

    Techniques Used: Expressing, Inhibition, Amplification

    IL-27 enhances macrophage APOBEC RNA expression . Monocyte-derived macrophages were treated 18 to 24 hours with IL-27 at 100 ng/mL or IFN-α at 10 ng/mL. Cells were lysed and processed for RNA following QIAGEN's RNeasy protocol, and gene transcription
    Figure Legend Snippet: IL-27 enhances macrophage APOBEC RNA expression . Monocyte-derived macrophages were treated 18 to 24 hours with IL-27 at 100 ng/mL or IFN-α at 10 ng/mL. Cells were lysed and processed for RNA following QIAGEN's RNeasy protocol, and gene transcription

    Techniques Used: RNA Expression, Derivative Assay

    IL-27 inhibition of T-cell HIV includes an IFN intermediate . (A) IL-27 induction of IFN- α in T cells at 0.5 to 4 hours (4 hours shown; n = 2), without corresponding increase in APOBEC3A until 24 hours but no increase in APOBEC3G (inset; n = 3).
    Figure Legend Snippet: IL-27 inhibition of T-cell HIV includes an IFN intermediate . (A) IL-27 induction of IFN- α in T cells at 0.5 to 4 hours (4 hours shown; n = 2), without corresponding increase in APOBEC3A until 24 hours but no increase in APOBEC3G (inset; n = 3).

    Techniques Used: Inhibition

    Blocking IL-27 signaling reverses inhibition of HIV infection . (A) Inhibition of IL-27 signal transduction by blocking JAK activation interrupts STAT1 and STAT3 phosphorylation. Macrophages were treated with JAK inhibitor at 1 or 10 nM for 1 hour before
    Figure Legend Snippet: Blocking IL-27 signaling reverses inhibition of HIV infection . (A) Inhibition of IL-27 signal transduction by blocking JAK activation interrupts STAT1 and STAT3 phosphorylation. Macrophages were treated with JAK inhibitor at 1 or 10 nM for 1 hour before

    Techniques Used: Blocking Assay, Inhibition, Infection, Transduction, Activation Assay

    14) Product Images from "Interleukin-27 inhibition of HIV-1 involves an intermediate induction of type I interferon"

    Article Title: Interleukin-27 inhibition of HIV-1 involves an intermediate induction of type I interferon

    Journal:

    doi: 10.1182/blood-2009-03-211540

    IL-27 inhibits HIV infection in macrophages . (A) Monocyte-derived macrophages were infected with 10 3 TCID 50 /mL BaL for 2 hours. Cells were washed, 10% DMEM added, and IL-27 at 0, 1, 10, and 100 ng/mL added only once after infection. Cells were fed every
    Figure Legend Snippet: IL-27 inhibits HIV infection in macrophages . (A) Monocyte-derived macrophages were infected with 10 3 TCID 50 /mL BaL for 2 hours. Cells were washed, 10% DMEM added, and IL-27 at 0, 1, 10, and 100 ng/mL added only once after infection. Cells were fed every

    Techniques Used: Infection, Derivative Assay

    IFN-α up-regulates IL-27 and IL-27 receptor mRNA expression . (A) Monocyte-derived macrophages (n = 3) were stimulated with IFN-α (10 ng/mL, 4 hours) in parallel with mock-treated cultures, and RNA was extracted and processed for microarray
    Figure Legend Snippet: IFN-α up-regulates IL-27 and IL-27 receptor mRNA expression . (A) Monocyte-derived macrophages (n = 3) were stimulated with IFN-α (10 ng/mL, 4 hours) in parallel with mock-treated cultures, and RNA was extracted and processed for microarray

    Techniques Used: Expressing, Derivative Assay, Microarray

    IL-27 induction of IFN-α and IFN-β supports APOBEC3A/G expression and inhibition of HIV . (A) PBMC were treated with IL-27 at 100 ng/mL for 30 minutes to 24 hours. RNA was reverse transcribed to cDNA, and amplification was performed using
    Figure Legend Snippet: IL-27 induction of IFN-α and IFN-β supports APOBEC3A/G expression and inhibition of HIV . (A) PBMC were treated with IL-27 at 100 ng/mL for 30 minutes to 24 hours. RNA was reverse transcribed to cDNA, and amplification was performed using

    Techniques Used: Expressing, Inhibition, Amplification

    IL-27 enhances macrophage APOBEC RNA expression . Monocyte-derived macrophages were treated 18 to 24 hours with IL-27 at 100 ng/mL or IFN-α at 10 ng/mL. Cells were lysed and processed for RNA following QIAGEN's RNeasy protocol, and gene transcription
    Figure Legend Snippet: IL-27 enhances macrophage APOBEC RNA expression . Monocyte-derived macrophages were treated 18 to 24 hours with IL-27 at 100 ng/mL or IFN-α at 10 ng/mL. Cells were lysed and processed for RNA following QIAGEN's RNeasy protocol, and gene transcription

    Techniques Used: RNA Expression, Derivative Assay

    IL-27 inhibition of T-cell HIV includes an IFN intermediate . (A) IL-27 induction of IFN- α in T cells at 0.5 to 4 hours (4 hours shown; n = 2), without corresponding increase in APOBEC3A until 24 hours but no increase in APOBEC3G (inset; n = 3).
    Figure Legend Snippet: IL-27 inhibition of T-cell HIV includes an IFN intermediate . (A) IL-27 induction of IFN- α in T cells at 0.5 to 4 hours (4 hours shown; n = 2), without corresponding increase in APOBEC3A until 24 hours but no increase in APOBEC3G (inset; n = 3).

    Techniques Used: Inhibition

    Blocking IL-27 signaling reverses inhibition of HIV infection . (A) Inhibition of IL-27 signal transduction by blocking JAK activation interrupts STAT1 and STAT3 phosphorylation. Macrophages were treated with JAK inhibitor at 1 or 10 nM for 1 hour before
    Figure Legend Snippet: Blocking IL-27 signaling reverses inhibition of HIV infection . (A) Inhibition of IL-27 signal transduction by blocking JAK activation interrupts STAT1 and STAT3 phosphorylation. Macrophages were treated with JAK inhibitor at 1 or 10 nM for 1 hour before

    Techniques Used: Blocking Assay, Inhibition, Infection, Transduction, Activation Assay

    15) Product Images from "Interleukin-27 inhibition of HIV-1 involves an intermediate induction of type I interferon"

    Article Title: Interleukin-27 inhibition of HIV-1 involves an intermediate induction of type I interferon

    Journal:

    doi: 10.1182/blood-2009-03-211540

    IL-27 inhibits HIV infection in macrophages . (A) Monocyte-derived macrophages were infected with 10 3 TCID 50 /mL BaL for 2 hours. Cells were washed, 10% DMEM added, and IL-27 at 0, 1, 10, and 100 ng/mL added only once after infection. Cells were fed every
    Figure Legend Snippet: IL-27 inhibits HIV infection in macrophages . (A) Monocyte-derived macrophages were infected with 10 3 TCID 50 /mL BaL for 2 hours. Cells were washed, 10% DMEM added, and IL-27 at 0, 1, 10, and 100 ng/mL added only once after infection. Cells were fed every

    Techniques Used: Infection, Derivative Assay

    IFN-α up-regulates IL-27 and IL-27 receptor mRNA expression . (A) Monocyte-derived macrophages (n = 3) were stimulated with IFN-α (10 ng/mL, 4 hours) in parallel with mock-treated cultures, and RNA was extracted and processed for microarray
    Figure Legend Snippet: IFN-α up-regulates IL-27 and IL-27 receptor mRNA expression . (A) Monocyte-derived macrophages (n = 3) were stimulated with IFN-α (10 ng/mL, 4 hours) in parallel with mock-treated cultures, and RNA was extracted and processed for microarray

    Techniques Used: Expressing, Derivative Assay, Microarray

    IL-27 induction of IFN-α and IFN-β supports APOBEC3A/G expression and inhibition of HIV . (A) PBMC were treated with IL-27 at 100 ng/mL for 30 minutes to 24 hours. RNA was reverse transcribed to cDNA, and amplification was performed using
    Figure Legend Snippet: IL-27 induction of IFN-α and IFN-β supports APOBEC3A/G expression and inhibition of HIV . (A) PBMC were treated with IL-27 at 100 ng/mL for 30 minutes to 24 hours. RNA was reverse transcribed to cDNA, and amplification was performed using

    Techniques Used: Expressing, Inhibition, Amplification

    IL-27 enhances macrophage APOBEC RNA expression . Monocyte-derived macrophages were treated 18 to 24 hours with IL-27 at 100 ng/mL or IFN-α at 10 ng/mL. Cells were lysed and processed for RNA following QIAGEN's RNeasy protocol, and gene transcription
    Figure Legend Snippet: IL-27 enhances macrophage APOBEC RNA expression . Monocyte-derived macrophages were treated 18 to 24 hours with IL-27 at 100 ng/mL or IFN-α at 10 ng/mL. Cells were lysed and processed for RNA following QIAGEN's RNeasy protocol, and gene transcription

    Techniques Used: RNA Expression, Derivative Assay

    IL-27 inhibition of T-cell HIV includes an IFN intermediate . (A) IL-27 induction of IFN- α in T cells at 0.5 to 4 hours (4 hours shown; n = 2), without corresponding increase in APOBEC3A until 24 hours but no increase in APOBEC3G (inset; n = 3).
    Figure Legend Snippet: IL-27 inhibition of T-cell HIV includes an IFN intermediate . (A) IL-27 induction of IFN- α in T cells at 0.5 to 4 hours (4 hours shown; n = 2), without corresponding increase in APOBEC3A until 24 hours but no increase in APOBEC3G (inset; n = 3).

    Techniques Used: Inhibition

    Blocking IL-27 signaling reverses inhibition of HIV infection . (A) Inhibition of IL-27 signal transduction by blocking JAK activation interrupts STAT1 and STAT3 phosphorylation. Macrophages were treated with JAK inhibitor at 1 or 10 nM for 1 hour before
    Figure Legend Snippet: Blocking IL-27 signaling reverses inhibition of HIV infection . (A) Inhibition of IL-27 signal transduction by blocking JAK activation interrupts STAT1 and STAT3 phosphorylation. Macrophages were treated with JAK inhibitor at 1 or 10 nM for 1 hour before

    Techniques Used: Blocking Assay, Inhibition, Infection, Transduction, Activation Assay

    16) Product Images from "Murine myeloid-derived suppressor cells are a source of elevated levels of interleukin-27 in early life and compromise control of bacterial infection"

    Article Title: Murine myeloid-derived suppressor cells are a source of elevated levels of interleukin-27 in early life and compromise control of bacterial infection

    Journal: Immunology and cell biology

    doi: 10.1111/imcb.12224

    Circulating IL-27 levels are elevated in neonates. ( a ) Blood was collected from mice in the indicated age groups and serum levels of IL-27 were measured by luminescent immunoassay. Each symbol represents an individual animal finding. Statistical significance was determined using unpaired t tests with Welch’s correction.
    Figure Legend Snippet: Circulating IL-27 levels are elevated in neonates. ( a ) Blood was collected from mice in the indicated age groups and serum levels of IL-27 were measured by luminescent immunoassay. Each symbol represents an individual animal finding. Statistical significance was determined using unpaired t tests with Welch’s correction.

    Techniques Used: Mouse Assay

    Isolated MDSCs produce IL-27. MDSCs were isolated from neonatal C57BL/6 mouse spleens as described in the Methods . ( a ) The isolated cells were labeled for surface-expressed Gr-1 and intracellular IL-27 and analyzed by flow cytometry. A representative dot plot relative to isotype controls is shown. ( b ) MDSC expression of the indicated genes relative to a no template control was determined by real time PCR. Six independent measurements using MDSCs obtained from pups ranging 5–8 days of age are combined and shown. ( c ) IL-27 concentration was determined in 24 h MDSC culture supernatants by ELISA. Seven independent measurements using MDSCs obtained from pups ranging 5–8 days of age are combined and shown. ( d ) The nitrite concentration in 48 h MDSC culture supernatants was measured. Five independent measurements using MDSCs obtained from pups ranging 5–10 days of age are combined and shown. ( e ) Arginase activity was measured relative to purified standards. Eight independent measurements using MDSCs obtained from pups ranging 5–8 days of age are combined and shown. ( f ) Oxidative burst was measured in MDSCs using Dihydrorhodamine 123. Data using MDSCs obtained from six day old pups representative of three separate measurements is shown.
    Figure Legend Snippet: Isolated MDSCs produce IL-27. MDSCs were isolated from neonatal C57BL/6 mouse spleens as described in the Methods . ( a ) The isolated cells were labeled for surface-expressed Gr-1 and intracellular IL-27 and analyzed by flow cytometry. A representative dot plot relative to isotype controls is shown. ( b ) MDSC expression of the indicated genes relative to a no template control was determined by real time PCR. Six independent measurements using MDSCs obtained from pups ranging 5–8 days of age are combined and shown. ( c ) IL-27 concentration was determined in 24 h MDSC culture supernatants by ELISA. Seven independent measurements using MDSCs obtained from pups ranging 5–8 days of age are combined and shown. ( d ) The nitrite concentration in 48 h MDSC culture supernatants was measured. Five independent measurements using MDSCs obtained from pups ranging 5–10 days of age are combined and shown. ( e ) Arginase activity was measured relative to purified standards. Eight independent measurements using MDSCs obtained from pups ranging 5–8 days of age are combined and shown. ( f ) Oxidative burst was measured in MDSCs using Dihydrorhodamine 123. Data using MDSCs obtained from six day old pups representative of three separate measurements is shown.

    Techniques Used: Isolation, Labeling, Flow Cytometry, Expressing, Real-time Polymerase Chain Reaction, Concentration Assay, Enzyme-linked Immunosorbent Assay, Activity Assay, Purification

    Gr-1 + cells that produce IL-27 are more abundant in neonates. Splenocytes from C57BL/6 mice at the indicated age were labeled for surface-expressed Gr-1 and intracellular IL-27 and analyzed by flow cytometry. ( a ) Representative dot plots relative to isotype controls are shown. ( b ) The combined percent Gr-1 + IL-27 + population from at least 8 mice at each age group are shown. Statistical significance was determined using an ANOVA and Tukey’s multiple comparison’s test.
    Figure Legend Snippet: Gr-1 + cells that produce IL-27 are more abundant in neonates. Splenocytes from C57BL/6 mice at the indicated age were labeled for surface-expressed Gr-1 and intracellular IL-27 and analyzed by flow cytometry. ( a ) Representative dot plots relative to isotype controls are shown. ( b ) The combined percent Gr-1 + IL-27 + population from at least 8 mice at each age group are shown. Statistical significance was determined using an ANOVA and Tukey’s multiple comparison’s test.

    Techniques Used: Mouse Assay, Labeling, Flow Cytometry

    MDSCs compromise macrophage control of E. coli . Bone marrow-derived macrophages were infected with Escherichia coli at an MOI of 20 in the presence or absence of MDSCs (5–13 days of age) at the indicated ratio. Recovery of intracellular bacteria was enumerated at 18 h and shown as mean log 10 recovered colony forming units ± standard error ( a ). Statistical significance in a combined six experiments was determined using a paired samples t test . ( b ) MDSCs were added to cultures where shown at the indicated ratio to macrophages. A combined four experiments are shown. Statistical significance was determined using paired samples t tests . ( c ) Macrophages were cultured as indicated ± MDSCs (ratio of 1:1 with macrophages) in the presence or absence of a soluble receptor to neutralize IL-27 (sIL27R). Five combined experiments are shown. Statistical significance was determined using paired samples t tests . ( d ) The concentration of TNF-α in culture supernatants was determined by ELISA. The presence of bacteria (Ec), sIL-27R, and ratio of MDSCs to macrophages (0.1X or 1X) is indicated. Results from an individual experiment representative of multiple are shown.
    Figure Legend Snippet: MDSCs compromise macrophage control of E. coli . Bone marrow-derived macrophages were infected with Escherichia coli at an MOI of 20 in the presence or absence of MDSCs (5–13 days of age) at the indicated ratio. Recovery of intracellular bacteria was enumerated at 18 h and shown as mean log 10 recovered colony forming units ± standard error ( a ). Statistical significance in a combined six experiments was determined using a paired samples t test . ( b ) MDSCs were added to cultures where shown at the indicated ratio to macrophages. A combined four experiments are shown. Statistical significance was determined using paired samples t tests . ( c ) Macrophages were cultured as indicated ± MDSCs (ratio of 1:1 with macrophages) in the presence or absence of a soluble receptor to neutralize IL-27 (sIL27R). Five combined experiments are shown. Statistical significance was determined using paired samples t tests . ( d ) The concentration of TNF-α in culture supernatants was determined by ELISA. The presence of bacteria (Ec), sIL-27R, and ratio of MDSCs to macrophages (0.1X or 1X) is indicated. Results from an individual experiment representative of multiple are shown.

    Techniques Used: Derivative Assay, Infection, Cell Culture, Concentration Assay, Enzyme-linked Immunosorbent Assay

    17) Product Images from "Noninfectious papilloma virus-like particles inhibit HIV-1 replication: implications for immune control of HIV-1 infection by IL-27"

    Article Title: Noninfectious papilloma virus-like particles inhibit HIV-1 replication: implications for immune control of HIV-1 infection by IL-27

    Journal:

    doi: 10.1182/blood-2006-02-001578

    Identification of IL-27 as a novel antiviral cytokine
    Figure Legend Snippet: Identification of IL-27 as a novel antiviral cytokine

    Techniques Used:

    IL-27 suppresses HIV replication. (A) PHA-stimulated CD4 + T cells infected with X4 virus and PHA-stimulated PBMCs infected with R5 virus were cultured for 7 days in the absence or presence of 12.5% of VLP-Sup from PBMCs. To neutralize IFNs (IFN-α
    Figure Legend Snippet: IL-27 suppresses HIV replication. (A) PHA-stimulated CD4 + T cells infected with X4 virus and PHA-stimulated PBMCs infected with R5 virus were cultured for 7 days in the absence or presence of 12.5% of VLP-Sup from PBMCs. To neutralize IFNs (IFN-α

    Techniques Used: Infection, Cell Culture

    18) Product Images from "Interleukin-27 inhibition of HIV-1 involves an intermediate induction of type I interferon"

    Article Title: Interleukin-27 inhibition of HIV-1 involves an intermediate induction of type I interferon

    Journal:

    doi: 10.1182/blood-2009-03-211540

    IL-27 inhibits HIV infection in macrophages . (A) Monocyte-derived macrophages were infected with 10 3 TCID 50 /mL BaL for 2 hours. Cells were washed, 10% DMEM added, and IL-27 at 0, 1, 10, and 100 ng/mL added only once after infection. Cells were fed every
    Figure Legend Snippet: IL-27 inhibits HIV infection in macrophages . (A) Monocyte-derived macrophages were infected with 10 3 TCID 50 /mL BaL for 2 hours. Cells were washed, 10% DMEM added, and IL-27 at 0, 1, 10, and 100 ng/mL added only once after infection. Cells were fed every

    Techniques Used: Infection, Derivative Assay

    IFN-α up-regulates IL-27 and IL-27 receptor mRNA expression . (A) Monocyte-derived macrophages (n = 3) were stimulated with IFN-α (10 ng/mL, 4 hours) in parallel with mock-treated cultures, and RNA was extracted and processed for microarray
    Figure Legend Snippet: IFN-α up-regulates IL-27 and IL-27 receptor mRNA expression . (A) Monocyte-derived macrophages (n = 3) were stimulated with IFN-α (10 ng/mL, 4 hours) in parallel with mock-treated cultures, and RNA was extracted and processed for microarray

    Techniques Used: Expressing, Derivative Assay, Microarray

    IL-27 induction of IFN-α and IFN-β supports APOBEC3A/G expression and inhibition of HIV . (A) PBMC were treated with IL-27 at 100 ng/mL for 30 minutes to 24 hours. RNA was reverse transcribed to cDNA, and amplification was performed using
    Figure Legend Snippet: IL-27 induction of IFN-α and IFN-β supports APOBEC3A/G expression and inhibition of HIV . (A) PBMC were treated with IL-27 at 100 ng/mL for 30 minutes to 24 hours. RNA was reverse transcribed to cDNA, and amplification was performed using

    Techniques Used: Expressing, Inhibition, Amplification

    IL-27 enhances macrophage APOBEC RNA expression . Monocyte-derived macrophages were treated 18 to 24 hours with IL-27 at 100 ng/mL or IFN-α at 10 ng/mL. Cells were lysed and processed for RNA following QIAGEN's RNeasy protocol, and gene transcription
    Figure Legend Snippet: IL-27 enhances macrophage APOBEC RNA expression . Monocyte-derived macrophages were treated 18 to 24 hours with IL-27 at 100 ng/mL or IFN-α at 10 ng/mL. Cells were lysed and processed for RNA following QIAGEN's RNeasy protocol, and gene transcription

    Techniques Used: RNA Expression, Derivative Assay

    IL-27 inhibition of T-cell HIV includes an IFN intermediate . (A) IL-27 induction of IFN- α in T cells at 0.5 to 4 hours (4 hours shown; n = 2), without corresponding increase in APOBEC3A until 24 hours but no increase in APOBEC3G (inset; n = 3).
    Figure Legend Snippet: IL-27 inhibition of T-cell HIV includes an IFN intermediate . (A) IL-27 induction of IFN- α in T cells at 0.5 to 4 hours (4 hours shown; n = 2), without corresponding increase in APOBEC3A until 24 hours but no increase in APOBEC3G (inset; n = 3).

    Techniques Used: Inhibition

    Blocking IL-27 signaling reverses inhibition of HIV infection . (A) Inhibition of IL-27 signal transduction by blocking JAK activation interrupts STAT1 and STAT3 phosphorylation. Macrophages were treated with JAK inhibitor at 1 or 10 nM for 1 hour before
    Figure Legend Snippet: Blocking IL-27 signaling reverses inhibition of HIV infection . (A) Inhibition of IL-27 signal transduction by blocking JAK activation interrupts STAT1 and STAT3 phosphorylation. Macrophages were treated with JAK inhibitor at 1 or 10 nM for 1 hour before

    Techniques Used: Blocking Assay, Inhibition, Infection, Transduction, Activation Assay

    19) Product Images from "The Inhibition of Group II Innate Lymphoid Cell Response by IL-27 in Allergic Rhinitis"

    Article Title: The Inhibition of Group II Innate Lymphoid Cell Response by IL-27 in Allergic Rhinitis

    Journal: Journal of Immunology Research

    doi: 10.1155/2020/6661524

    The IL-27 receptor expression by ILC2 regulated by IL-27. (a, b) The mRNA expression of gp130 and WSX-1 by ILC2 after IL-27 stimulation by polymerase chain reaction. (c) The protein expression of gp130 and WSX-1 by ILC2 after IL-27 stimulation by flow cytometry. Three independent tests were performed for every experiment. ∗ Compared with IL-27 (10 ng/mL), P
    Figure Legend Snippet: The IL-27 receptor expression by ILC2 regulated by IL-27. (a, b) The mRNA expression of gp130 and WSX-1 by ILC2 after IL-27 stimulation by polymerase chain reaction. (c) The protein expression of gp130 and WSX-1 by ILC2 after IL-27 stimulation by flow cytometry. Three independent tests were performed for every experiment. ∗ Compared with IL-27 (10 ng/mL), P

    Techniques Used: Expressing, Polymerase Chain Reaction, Flow Cytometry

    IL-27 inhibited ILC2 cell proliferation and cytokine expression. (a) Proliferation of ILC2 was assessed by tritiated thymidine incorporation under IL-27 stimulation. (b, c) The mRNA expression of GATA-3 and ROR α by ILC2 detected by PCR. (d, e) Type II cytokine protein expression by ILCA2 measured by ELISA. Three independent tests were performed for every experiment. ∗ Compared with PBS, P
    Figure Legend Snippet: IL-27 inhibited ILC2 cell proliferation and cytokine expression. (a) Proliferation of ILC2 was assessed by tritiated thymidine incorporation under IL-27 stimulation. (b, c) The mRNA expression of GATA-3 and ROR α by ILC2 detected by PCR. (d, e) Type II cytokine protein expression by ILCA2 measured by ELISA. Three independent tests were performed for every experiment. ∗ Compared with PBS, P

    Techniques Used: Expressing, Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

    IL-27 inhibits allergic responses in mouse model. (a) Levels of Der p1-specific IgE by enzyme-linked immunosorbent assay after IL-27 stimulation. (b, c) The proportions of nasal ILC2 in total cells of inferior turbinate tissue after IL-27 treatment by flow cytometry. (d–f) The proportions of nasal IL-5+ILC2 and IL-13+ILC2 in total cells of inferior turbinate tissue. After IL-27 treatment by flow cytometry. Ten mice were allocated into every group. Three independent tests were performed for every experiment. ∗ Compared with PBS, P
    Figure Legend Snippet: IL-27 inhibits allergic responses in mouse model. (a) Levels of Der p1-specific IgE by enzyme-linked immunosorbent assay after IL-27 stimulation. (b, c) The proportions of nasal ILC2 in total cells of inferior turbinate tissue after IL-27 treatment by flow cytometry. (d–f) The proportions of nasal IL-5+ILC2 and IL-13+ILC2 in total cells of inferior turbinate tissue. After IL-27 treatment by flow cytometry. Ten mice were allocated into every group. Three independent tests were performed for every experiment. ∗ Compared with PBS, P

    Techniques Used: Enzyme-linked Immunosorbent Assay, Flow Cytometry, Mouse Assay

    The serum expression of IL-27 and the proportion of ILC2 in PBMCs between AR and controls. (a, b) Expression of serum IL-27 mRNA and protein levels between AR and controls by polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. (c) Frequency of ILC2 in PBMCs between controls and AR as well as isotype and FMO controls by flow cytometry. AR: allergic rhinitis; FMO: fluorescence minus one; HC: healthy control; ILC2: group II innate lymphoid cells; PBMC: peripheral blood mononuclear cells.
    Figure Legend Snippet: The serum expression of IL-27 and the proportion of ILC2 in PBMCs between AR and controls. (a, b) Expression of serum IL-27 mRNA and protein levels between AR and controls by polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. (c) Frequency of ILC2 in PBMCs between controls and AR as well as isotype and FMO controls by flow cytometry. AR: allergic rhinitis; FMO: fluorescence minus one; HC: healthy control; ILC2: group II innate lymphoid cells; PBMC: peripheral blood mononuclear cells.

    Techniques Used: Expressing, Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Fluorescence

    Correlation between of IL-27 protein expression, the proportion of ILC2 in PBMCs, and TNSS score in AR. (a, b) Correlation between IL-27 protein levels and ILC2 frequency and TNSS score. (c) Correlation between ILC2 frequency and TNSS score. AR: allergic rhinitis; ILC2: group II innate lymphoid cells; PBMC: peripheral blood mononuclear cells; TNSS: total nasal symptom score.
    Figure Legend Snippet: Correlation between of IL-27 protein expression, the proportion of ILC2 in PBMCs, and TNSS score in AR. (a, b) Correlation between IL-27 protein levels and ILC2 frequency and TNSS score. (c) Correlation between ILC2 frequency and TNSS score. AR: allergic rhinitis; ILC2: group II innate lymphoid cells; PBMC: peripheral blood mononuclear cells; TNSS: total nasal symptom score.

    Techniques Used: Expressing

    20) Product Images from "Murine myeloid-derived suppressor cells are a source of elevated levels of interleukin-27 in early life and compromise control of bacterial infection"

    Article Title: Murine myeloid-derived suppressor cells are a source of elevated levels of interleukin-27 in early life and compromise control of bacterial infection

    Journal: Immunology and cell biology

    doi: 10.1111/imcb.12224

    Circulating IL-27 levels are elevated in neonates. ( a ) Blood was collected from mice in the indicated age groups and serum levels of IL-27 were measured by luminescent immunoassay. Each symbol represents an individual animal finding. Statistical significance was determined using unpaired t tests with Welch’s correction.
    Figure Legend Snippet: Circulating IL-27 levels are elevated in neonates. ( a ) Blood was collected from mice in the indicated age groups and serum levels of IL-27 were measured by luminescent immunoassay. Each symbol represents an individual animal finding. Statistical significance was determined using unpaired t tests with Welch’s correction.

    Techniques Used: Mouse Assay

    Isolated MDSCs produce IL-27. MDSCs were isolated from neonatal C57BL/6 mouse spleens as described in the Methods . ( a ) The isolated cells were labeled for surface-expressed Gr-1 and intracellular IL-27 and analyzed by flow cytometry. A representative dot plot relative to isotype controls is shown. ( b ) MDSC expression of the indicated genes relative to a no template control was determined by real time PCR. Six independent measurements using MDSCs obtained from pups ranging 5–8 days of age are combined and shown. ( c ) IL-27 concentration was determined in 24 h MDSC culture supernatants by ELISA. Seven independent measurements using MDSCs obtained from pups ranging 5–8 days of age are combined and shown. ( d ) The nitrite concentration in 48 h MDSC culture supernatants was measured. Five independent measurements using MDSCs obtained from pups ranging 5–10 days of age are combined and shown. ( e ) Arginase activity was measured relative to purified standards. Eight independent measurements using MDSCs obtained from pups ranging 5–8 days of age are combined and shown. ( f ) Oxidative burst was measured in MDSCs using Dihydrorhodamine 123. Data using MDSCs obtained from six day old pups representative of three separate measurements is shown.
    Figure Legend Snippet: Isolated MDSCs produce IL-27. MDSCs were isolated from neonatal C57BL/6 mouse spleens as described in the Methods . ( a ) The isolated cells were labeled for surface-expressed Gr-1 and intracellular IL-27 and analyzed by flow cytometry. A representative dot plot relative to isotype controls is shown. ( b ) MDSC expression of the indicated genes relative to a no template control was determined by real time PCR. Six independent measurements using MDSCs obtained from pups ranging 5–8 days of age are combined and shown. ( c ) IL-27 concentration was determined in 24 h MDSC culture supernatants by ELISA. Seven independent measurements using MDSCs obtained from pups ranging 5–8 days of age are combined and shown. ( d ) The nitrite concentration in 48 h MDSC culture supernatants was measured. Five independent measurements using MDSCs obtained from pups ranging 5–10 days of age are combined and shown. ( e ) Arginase activity was measured relative to purified standards. Eight independent measurements using MDSCs obtained from pups ranging 5–8 days of age are combined and shown. ( f ) Oxidative burst was measured in MDSCs using Dihydrorhodamine 123. Data using MDSCs obtained from six day old pups representative of three separate measurements is shown.

    Techniques Used: Isolation, Labeling, Flow Cytometry, Expressing, Real-time Polymerase Chain Reaction, Concentration Assay, Enzyme-linked Immunosorbent Assay, Activity Assay, Purification

    Gr-1 + cells that produce IL-27 are more abundant in neonates. Splenocytes from C57BL/6 mice at the indicated age were labeled for surface-expressed Gr-1 and intracellular IL-27 and analyzed by flow cytometry. ( a ) Representative dot plots relative to isotype controls are shown. ( b ) The combined percent Gr-1 + IL-27 + population from at least 8 mice at each age group are shown. Statistical significance was determined using an ANOVA and Tukey’s multiple comparison’s test.
    Figure Legend Snippet: Gr-1 + cells that produce IL-27 are more abundant in neonates. Splenocytes from C57BL/6 mice at the indicated age were labeled for surface-expressed Gr-1 and intracellular IL-27 and analyzed by flow cytometry. ( a ) Representative dot plots relative to isotype controls are shown. ( b ) The combined percent Gr-1 + IL-27 + population from at least 8 mice at each age group are shown. Statistical significance was determined using an ANOVA and Tukey’s multiple comparison’s test.

    Techniques Used: Mouse Assay, Labeling, Flow Cytometry

    MDSCs compromise macrophage control of E. coli . Bone marrow-derived macrophages were infected with Escherichia coli at an MOI of 20 in the presence or absence of MDSCs (5–13 days of age) at the indicated ratio. Recovery of intracellular bacteria was enumerated at 18 h and shown as mean log 10 recovered colony forming units ± standard error ( a ). Statistical significance in a combined six experiments was determined using a paired samples t test . ( b ) MDSCs were added to cultures where shown at the indicated ratio to macrophages. A combined four experiments are shown. Statistical significance was determined using paired samples t tests . ( c ) Macrophages were cultured as indicated ± MDSCs (ratio of 1:1 with macrophages) in the presence or absence of a soluble receptor to neutralize IL-27 (sIL27R). Five combined experiments are shown. Statistical significance was determined using paired samples t tests . ( d ) The concentration of TNF-α in culture supernatants was determined by ELISA. The presence of bacteria (Ec), sIL-27R, and ratio of MDSCs to macrophages (0.1X or 1X) is indicated. Results from an individual experiment representative of multiple are shown.
    Figure Legend Snippet: MDSCs compromise macrophage control of E. coli . Bone marrow-derived macrophages were infected with Escherichia coli at an MOI of 20 in the presence or absence of MDSCs (5–13 days of age) at the indicated ratio. Recovery of intracellular bacteria was enumerated at 18 h and shown as mean log 10 recovered colony forming units ± standard error ( a ). Statistical significance in a combined six experiments was determined using a paired samples t test . ( b ) MDSCs were added to cultures where shown at the indicated ratio to macrophages. A combined four experiments are shown. Statistical significance was determined using paired samples t tests . ( c ) Macrophages were cultured as indicated ± MDSCs (ratio of 1:1 with macrophages) in the presence or absence of a soluble receptor to neutralize IL-27 (sIL27R). Five combined experiments are shown. Statistical significance was determined using paired samples t tests . ( d ) The concentration of TNF-α in culture supernatants was determined by ELISA. The presence of bacteria (Ec), sIL-27R, and ratio of MDSCs to macrophages (0.1X or 1X) is indicated. Results from an individual experiment representative of multiple are shown.

    Techniques Used: Derivative Assay, Infection, Cell Culture, Concentration Assay, Enzyme-linked Immunosorbent Assay

    21) Product Images from "Interleukin-27 inhibition of HIV-1 involves an intermediate induction of type I interferon"

    Article Title: Interleukin-27 inhibition of HIV-1 involves an intermediate induction of type I interferon

    Journal:

    doi: 10.1182/blood-2009-03-211540

    IL-27 inhibits HIV infection in macrophages . (A) Monocyte-derived macrophages were infected with 10 3 TCID 50 /mL BaL for 2 hours. Cells were washed, 10% DMEM added, and IL-27 at 0, 1, 10, and 100 ng/mL added only once after infection. Cells were fed every
    Figure Legend Snippet: IL-27 inhibits HIV infection in macrophages . (A) Monocyte-derived macrophages were infected with 10 3 TCID 50 /mL BaL for 2 hours. Cells were washed, 10% DMEM added, and IL-27 at 0, 1, 10, and 100 ng/mL added only once after infection. Cells were fed every

    Techniques Used: Infection, Derivative Assay

    IFN-α up-regulates IL-27 and IL-27 receptor mRNA expression . (A) Monocyte-derived macrophages (n = 3) were stimulated with IFN-α (10 ng/mL, 4 hours) in parallel with mock-treated cultures, and RNA was extracted and processed for microarray
    Figure Legend Snippet: IFN-α up-regulates IL-27 and IL-27 receptor mRNA expression . (A) Monocyte-derived macrophages (n = 3) were stimulated with IFN-α (10 ng/mL, 4 hours) in parallel with mock-treated cultures, and RNA was extracted and processed for microarray

    Techniques Used: Expressing, Derivative Assay, Microarray

    IL-27 induction of IFN-α and IFN-β supports APOBEC3A/G expression and inhibition of HIV . (A) PBMC were treated with IL-27 at 100 ng/mL for 30 minutes to 24 hours. RNA was reverse transcribed to cDNA, and amplification was performed using
    Figure Legend Snippet: IL-27 induction of IFN-α and IFN-β supports APOBEC3A/G expression and inhibition of HIV . (A) PBMC were treated with IL-27 at 100 ng/mL for 30 minutes to 24 hours. RNA was reverse transcribed to cDNA, and amplification was performed using

    Techniques Used: Expressing, Inhibition, Amplification

    IL-27 enhances macrophage APOBEC RNA expression . Monocyte-derived macrophages were treated 18 to 24 hours with IL-27 at 100 ng/mL or IFN-α at 10 ng/mL. Cells were lysed and processed for RNA following QIAGEN's RNeasy protocol, and gene transcription
    Figure Legend Snippet: IL-27 enhances macrophage APOBEC RNA expression . Monocyte-derived macrophages were treated 18 to 24 hours with IL-27 at 100 ng/mL or IFN-α at 10 ng/mL. Cells were lysed and processed for RNA following QIAGEN's RNeasy protocol, and gene transcription

    Techniques Used: RNA Expression, Derivative Assay

    IL-27 inhibition of T-cell HIV includes an IFN intermediate . (A) IL-27 induction of IFN- α in T cells at 0.5 to 4 hours (4 hours shown; n = 2), without corresponding increase in APOBEC3A until 24 hours but no increase in APOBEC3G (inset; n = 3).
    Figure Legend Snippet: IL-27 inhibition of T-cell HIV includes an IFN intermediate . (A) IL-27 induction of IFN- α in T cells at 0.5 to 4 hours (4 hours shown; n = 2), without corresponding increase in APOBEC3A until 24 hours but no increase in APOBEC3G (inset; n = 3).

    Techniques Used: Inhibition

    Blocking IL-27 signaling reverses inhibition of HIV infection . (A) Inhibition of IL-27 signal transduction by blocking JAK activation interrupts STAT1 and STAT3 phosphorylation. Macrophages were treated with JAK inhibitor at 1 or 10 nM for 1 hour before
    Figure Legend Snippet: Blocking IL-27 signaling reverses inhibition of HIV infection . (A) Inhibition of IL-27 signal transduction by blocking JAK activation interrupts STAT1 and STAT3 phosphorylation. Macrophages were treated with JAK inhibitor at 1 or 10 nM for 1 hour before

    Techniques Used: Blocking Assay, Inhibition, Infection, Transduction, Activation Assay

    22) Product Images from "Interleukin-27 inhibition of HIV-1 involves an intermediate induction of type I interferon"

    Article Title: Interleukin-27 inhibition of HIV-1 involves an intermediate induction of type I interferon

    Journal:

    doi: 10.1182/blood-2009-03-211540

    IL-27 inhibits HIV infection in macrophages . (A) Monocyte-derived macrophages were infected with 10 3 TCID 50 /mL BaL for 2 hours. Cells were washed, 10% DMEM added, and IL-27 at 0, 1, 10, and 100 ng/mL added only once after infection. Cells were fed every
    Figure Legend Snippet: IL-27 inhibits HIV infection in macrophages . (A) Monocyte-derived macrophages were infected with 10 3 TCID 50 /mL BaL for 2 hours. Cells were washed, 10% DMEM added, and IL-27 at 0, 1, 10, and 100 ng/mL added only once after infection. Cells were fed every

    Techniques Used: Infection, Derivative Assay

    IFN-α up-regulates IL-27 and IL-27 receptor mRNA expression . (A) Monocyte-derived macrophages (n = 3) were stimulated with IFN-α (10 ng/mL, 4 hours) in parallel with mock-treated cultures, and RNA was extracted and processed for microarray
    Figure Legend Snippet: IFN-α up-regulates IL-27 and IL-27 receptor mRNA expression . (A) Monocyte-derived macrophages (n = 3) were stimulated with IFN-α (10 ng/mL, 4 hours) in parallel with mock-treated cultures, and RNA was extracted and processed for microarray

    Techniques Used: Expressing, Derivative Assay, Microarray

    IL-27 induction of IFN-α and IFN-β supports APOBEC3A/G expression and inhibition of HIV . (A) PBMC were treated with IL-27 at 100 ng/mL for 30 minutes to 24 hours. RNA was reverse transcribed to cDNA, and amplification was performed using
    Figure Legend Snippet: IL-27 induction of IFN-α and IFN-β supports APOBEC3A/G expression and inhibition of HIV . (A) PBMC were treated with IL-27 at 100 ng/mL for 30 minutes to 24 hours. RNA was reverse transcribed to cDNA, and amplification was performed using

    Techniques Used: Expressing, Inhibition, Amplification

    IL-27 enhances macrophage APOBEC RNA expression . Monocyte-derived macrophages were treated 18 to 24 hours with IL-27 at 100 ng/mL or IFN-α at 10 ng/mL. Cells were lysed and processed for RNA following QIAGEN's RNeasy protocol, and gene transcription
    Figure Legend Snippet: IL-27 enhances macrophage APOBEC RNA expression . Monocyte-derived macrophages were treated 18 to 24 hours with IL-27 at 100 ng/mL or IFN-α at 10 ng/mL. Cells were lysed and processed for RNA following QIAGEN's RNeasy protocol, and gene transcription

    Techniques Used: RNA Expression, Derivative Assay

    IL-27 inhibition of T-cell HIV includes an IFN intermediate . (A) IL-27 induction of IFN- α in T cells at 0.5 to 4 hours (4 hours shown; n = 2), without corresponding increase in APOBEC3A until 24 hours but no increase in APOBEC3G (inset; n = 3).
    Figure Legend Snippet: IL-27 inhibition of T-cell HIV includes an IFN intermediate . (A) IL-27 induction of IFN- α in T cells at 0.5 to 4 hours (4 hours shown; n = 2), without corresponding increase in APOBEC3A until 24 hours but no increase in APOBEC3G (inset; n = 3).

    Techniques Used: Inhibition

    Blocking IL-27 signaling reverses inhibition of HIV infection . (A) Inhibition of IL-27 signal transduction by blocking JAK activation interrupts STAT1 and STAT3 phosphorylation. Macrophages were treated with JAK inhibitor at 1 or 10 nM for 1 hour before
    Figure Legend Snippet: Blocking IL-27 signaling reverses inhibition of HIV infection . (A) Inhibition of IL-27 signal transduction by blocking JAK activation interrupts STAT1 and STAT3 phosphorylation. Macrophages were treated with JAK inhibitor at 1 or 10 nM for 1 hour before

    Techniques Used: Blocking Assay, Inhibition, Infection, Transduction, Activation Assay

    23) Product Images from "Autocrine TGF-β1 Maintains the Stability of Foxp3+ Regulatory T Cells via IL-12Rβ2 Downregulation"

    Article Title: Autocrine TGF-β1 Maintains the Stability of Foxp3+ Regulatory T Cells via IL-12Rβ2 Downregulation

    Journal: Biomolecules

    doi: 10.3390/biom10060819

    Ablation of TGF-β1 induces excessive IFN-γ production of Treg cells after IL-12 stimulation. ( A ) Sorted Treg cells from Foxp3 YFP-Cre Tgfb1 fl/+ and Foxp3 YFP-Cre Tgfb1 fl/fl mice were stimulated with anti-CD3ε with (+ group) or without IL-12 (− group). Representative contour plots and quantification of IFN-γ- and IL-17A-expressing cells. ( B ) Sorted Treg cells from Foxp3 YFP-Cre Tgfb1 fl/+ and Foxp3 YFP-Cre Tgfb1 fl/fl mice were stimulated with anti-CD3ε with (+ group) or without IL-27 (− group). Representative contour plots and quantification of IFN-γ- and IL-17A-expressing cells. Data are representative of four ( A , B ) independent experiments, and values are expressed as the mean ± SD ( A , B ). * p ≤ 0.05, ** p ≤ 0.01, and **** p ≤ 0.0001 compared to TGF-β1-sufficient Treg cells based on Student’s t -test. ( C ) Schematic model of the proposed mechanism.
    Figure Legend Snippet: Ablation of TGF-β1 induces excessive IFN-γ production of Treg cells after IL-12 stimulation. ( A ) Sorted Treg cells from Foxp3 YFP-Cre Tgfb1 fl/+ and Foxp3 YFP-Cre Tgfb1 fl/fl mice were stimulated with anti-CD3ε with (+ group) or without IL-12 (− group). Representative contour plots and quantification of IFN-γ- and IL-17A-expressing cells. ( B ) Sorted Treg cells from Foxp3 YFP-Cre Tgfb1 fl/+ and Foxp3 YFP-Cre Tgfb1 fl/fl mice were stimulated with anti-CD3ε with (+ group) or without IL-27 (− group). Representative contour plots and quantification of IFN-γ- and IL-17A-expressing cells. Data are representative of four ( A , B ) independent experiments, and values are expressed as the mean ± SD ( A , B ). * p ≤ 0.05, ** p ≤ 0.01, and **** p ≤ 0.0001 compared to TGF-β1-sufficient Treg cells based on Student’s t -test. ( C ) Schematic model of the proposed mechanism.

    Techniques Used: Mouse Assay, Expressing

    24) Product Images from "Profiles of Long Non-Coding RNAs and mRNA Expression in Human Macrophages Regulated by Interleukin-27"

    Article Title: Profiles of Long Non-Coding RNAs and mRNA Expression in Human Macrophages Regulated by Interleukin-27

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms20246207

    The differential expression profiles for Interleukin-27 (IL-27 regulated mRNAs. ( A ) The hierarchical clustering of differentially regulated mRNAs for iMac vs. mMac in the IMAC experiment. ( B ) The hierarchical clustering of differentially regulated mRNAs for Abi vs. Ab in the ABI experiment. ( C ) Venn diagram indicates the number of overlapping and non-overlapping differential mRNAs in the IMAC and ABI experiments. Values in the heatmap are the fold change for each donor. The color scale shown at the right illustrates the relative expression level of the indicated mRNA in each sample: green denotes downregulated (log 2 fold change
    Figure Legend Snippet: The differential expression profiles for Interleukin-27 (IL-27 regulated mRNAs. ( A ) The hierarchical clustering of differentially regulated mRNAs for iMac vs. mMac in the IMAC experiment. ( B ) The hierarchical clustering of differentially regulated mRNAs for Abi vs. Ab in the ABI experiment. ( C ) Venn diagram indicates the number of overlapping and non-overlapping differential mRNAs in the IMAC and ABI experiments. Values in the heatmap are the fold change for each donor. The color scale shown at the right illustrates the relative expression level of the indicated mRNA in each sample: green denotes downregulated (log 2 fold change

    Techniques Used: Expressing

    The differential expression profiles for Interleukin (IL)-27 regulated long non-coding RNAs (lncRNAs). ( A ) The hierarchical clustering of differentially regulated lncRNAs for iMac vs. mMac in the IMAC experiment. ( B ) The hierarchical clustering of differentially regulated lncRNAs for Abi vs. Ab in the ABI experiment. ( C ) Venn diagram indicates the number of overlapping and non-overlapping differential lncRNAs in the IMAC and ABI experiments. Values in the heatmap indicate the fold change for each donor. The color scale shown at the right illustrates the relative expression level of the indicated lncRNA in each sample: green denotes downregulated (log 2 fold change
    Figure Legend Snippet: The differential expression profiles for Interleukin (IL)-27 regulated long non-coding RNAs (lncRNAs). ( A ) The hierarchical clustering of differentially regulated lncRNAs for iMac vs. mMac in the IMAC experiment. ( B ) The hierarchical clustering of differentially regulated lncRNAs for Abi vs. Ab in the ABI experiment. ( C ) Venn diagram indicates the number of overlapping and non-overlapping differential lncRNAs in the IMAC and ABI experiments. Values in the heatmap indicate the fold change for each donor. The color scale shown at the right illustrates the relative expression level of the indicated lncRNA in each sample: green denotes downregulated (log 2 fold change

    Techniques Used: Expressing

    25) Product Images from "PD-L1 up-regulation restrains Th17 cell differentiation in STAT3 loss- and STAT1 gain-of-function patients"

    Article Title: PD-L1 up-regulation restrains Th17 cell differentiation in STAT3 loss- and STAT1 gain-of-function patients

    Journal: The Journal of Experimental Medicine

    doi: 10.1084/jem.20161427

    STAT3 LOF and STAT1 GOF cells have enhanced pSTAT1 that is partially dependent on impaired induction of SOCS3. (A) pSTAT1 (left) and pSTAT3 (right) in PBMCs after stimulation with IL-6, IL-21, or IL-27 for 15 min. (B) Total STAT1 in PBMCS before stimulation (left) and after cytokine stimulation (right) for 15 min. (C and D) SOCS3 and SOCS1 mRNA (C) and SOCS3 protein (D) levels in naive CD4 T cells after stimulation with IL-6, IL-21, or IL-27 for 1 h. (E and F) Naive (CD45RO − ) CD4 + T cells were transfected with SOCS3–GFP or GFP–control or SOCS3 –RNAi or scramble RNAi–control. IL-27 was added 5 h after transfection, and pSTAT1 levels were analyzed 18–24 h later. One-way ANOVA (A–D) and paired and Student’s t tests (E and F) were performed. *, P
    Figure Legend Snippet: STAT3 LOF and STAT1 GOF cells have enhanced pSTAT1 that is partially dependent on impaired induction of SOCS3. (A) pSTAT1 (left) and pSTAT3 (right) in PBMCs after stimulation with IL-6, IL-21, or IL-27 for 15 min. (B) Total STAT1 in PBMCS before stimulation (left) and after cytokine stimulation (right) for 15 min. (C and D) SOCS3 and SOCS1 mRNA (C) and SOCS3 protein (D) levels in naive CD4 T cells after stimulation with IL-6, IL-21, or IL-27 for 1 h. (E and F) Naive (CD45RO − ) CD4 + T cells were transfected with SOCS3–GFP or GFP–control or SOCS3 –RNAi or scramble RNAi–control. IL-27 was added 5 h after transfection, and pSTAT1 levels were analyzed 18–24 h later. One-way ANOVA (A–D) and paired and Student’s t tests (E and F) were performed. *, P

    Techniques Used: Transfection

    High pSTAT1 drives increased PD-L1 expression in STAT3 LOF and STAT1 GOF cells. (A) PD-L1 expression on naive (CD45RO − ) T cells from HCs and patients after 24 h of stimulation with IL-27. Naive (CD45RO − ) CD4 + T cells were transfected with STAT1 RNAi or scramble RNAi (B and C), SOCS3-GFP or GFP-control (D), or SOCS3 RNAi or scramble RNAi (E). (B) STAT1 expression after transfection with STAT1 RNAi. (C–E) PD-L1 expression 41 h after IL-27 stimulation. One-way ANOVA (A) and paired Student’s t tests (C–E) were performed. *, P
    Figure Legend Snippet: High pSTAT1 drives increased PD-L1 expression in STAT3 LOF and STAT1 GOF cells. (A) PD-L1 expression on naive (CD45RO − ) T cells from HCs and patients after 24 h of stimulation with IL-27. Naive (CD45RO − ) CD4 + T cells were transfected with STAT1 RNAi or scramble RNAi (B and C), SOCS3-GFP or GFP-control (D), or SOCS3 RNAi or scramble RNAi (E). (B) STAT1 expression after transfection with STAT1 RNAi. (C–E) PD-L1 expression 41 h after IL-27 stimulation. One-way ANOVA (A) and paired Student’s t tests (C–E) were performed. *, P

    Techniques Used: Expressing, Transfection

    26) Product Images from "Interferon‐β regulates the production of IL‐10 by toll‐like receptor‐activated microglia. Interferon‐ β regulates the production of IL‐10 by toll‐like receptor‐activated microglia"

    Article Title: Interferon‐β regulates the production of IL‐10 by toll‐like receptor‐activated microglia. Interferon‐ β regulates the production of IL‐10 by toll‐like receptor‐activated microglia

    Journal: Glia

    doi: 10.1002/glia.23172

    TLR3 co‐stimulation of microglia does not lead to overshooting responses. (a–k) WT microglial cells were stimulated for 24 hr with TLR2 or TLR3 agonists alone or in combination. Cell culture supernatants were collected and cytokine production was measured by multiplex assay (IL‐12p70, IL‐23, IL‐27, IL‐22, IFN‐γ, IL‐4, and IL‐5) or ELISA (TNF, IL‐6, IFN‐β, and IL‐1β). Significant statistical differences relative to TLR2 are represented by *; to TLR3 by #. (l) WT and IFNAR−/− microglial cells were stimulated for 24 hr with the TLR2 or TLR3 agonists alone or in combination. Cell culture supernatants were collected and IL‐27 production was measured by multiplex. (m) WT microglial cells were stimulated for 24 hr with the TLR2 agonist, IFN‐β or IL‐27 alone or in combination. Cell culture supernatants were collected and IL‐10 production was measured by ELISA. Significant statistical differences relative to TLR2 are represented by *; to TLR2 + IFN‐β by #; to TLR2 + IL‐27 by $. The detection limit for each cytokine is represented as a dotted line in each graph. Represented are the mean ± SD for triplicate wells per condition set after mixed cultures generated from independent mice. Statistical differences were assessed by student's t test or one‐way ANOVA. Significant statistical differences are represented by one symbol, p
    Figure Legend Snippet: TLR3 co‐stimulation of microglia does not lead to overshooting responses. (a–k) WT microglial cells were stimulated for 24 hr with TLR2 or TLR3 agonists alone or in combination. Cell culture supernatants were collected and cytokine production was measured by multiplex assay (IL‐12p70, IL‐23, IL‐27, IL‐22, IFN‐γ, IL‐4, and IL‐5) or ELISA (TNF, IL‐6, IFN‐β, and IL‐1β). Significant statistical differences relative to TLR2 are represented by *; to TLR3 by #. (l) WT and IFNAR−/− microglial cells were stimulated for 24 hr with the TLR2 or TLR3 agonists alone or in combination. Cell culture supernatants were collected and IL‐27 production was measured by multiplex. (m) WT microglial cells were stimulated for 24 hr with the TLR2 agonist, IFN‐β or IL‐27 alone or in combination. Cell culture supernatants were collected and IL‐10 production was measured by ELISA. Significant statistical differences relative to TLR2 are represented by *; to TLR2 + IFN‐β by #; to TLR2 + IL‐27 by $. The detection limit for each cytokine is represented as a dotted line in each graph. Represented are the mean ± SD for triplicate wells per condition set after mixed cultures generated from independent mice. Statistical differences were assessed by student's t test or one‐way ANOVA. Significant statistical differences are represented by one symbol, p

    Techniques Used: Cell Culture, Multiplex Assay, Enzyme-linked Immunosorbent Assay, Generated, Mouse Assay

    Inflammatory landscape of TLR‐stimulated microglia. (a–k). Primary microglial cell cultures were left unstimulated or stimulated for 24 hr with chemical TLR agonists for TLR2, TLR3, TLR4, or TLR9, as described in Section 2 . Cell culture supernatants were collected and cytokine production was measured by multiplex assay (IL‐10, TNF, IL‐12p70, IL‐23, IL‐27, IL‐22, IFN‐γ, IL‐4, and IL‐5) or ELISA (IL‐6 and IFN‐β). Unstimulated cells did not produce detectable amounts of cytokines. The detection limit for each cytokine is represented as a dotted line in each graph. Represented are the mean ± SD for triplicate wells per condition set after mixed cultures generated from independent mice. Statistical differences were assessed by one‐way ANOVA or student's t test. Significant statistical differences relative to TLR2 are represented by *; to TLR3 by #; to TLR4 by $. One symbol, p
    Figure Legend Snippet: Inflammatory landscape of TLR‐stimulated microglia. (a–k). Primary microglial cell cultures were left unstimulated or stimulated for 24 hr with chemical TLR agonists for TLR2, TLR3, TLR4, or TLR9, as described in Section 2 . Cell culture supernatants were collected and cytokine production was measured by multiplex assay (IL‐10, TNF, IL‐12p70, IL‐23, IL‐27, IL‐22, IFN‐γ, IL‐4, and IL‐5) or ELISA (IL‐6 and IFN‐β). Unstimulated cells did not produce detectable amounts of cytokines. The detection limit for each cytokine is represented as a dotted line in each graph. Represented are the mean ± SD for triplicate wells per condition set after mixed cultures generated from independent mice. Statistical differences were assessed by one‐way ANOVA or student's t test. Significant statistical differences relative to TLR2 are represented by *; to TLR3 by #; to TLR4 by $. One symbol, p

    Techniques Used: Cell Culture, Multiplex Assay, Enzyme-linked Immunosorbent Assay, Generated, Mouse Assay

    27) Product Images from "Profiles of MicroRNAs in Interleukin–27-Induced HIV-Resistant T Cells: Identification of a Novel Antiviral MicroRNA"

    Article Title: Profiles of MicroRNAs in Interleukin–27-Induced HIV-Resistant T Cells: Identification of a Novel Antiviral MicroRNA

    Journal: Journal of Acquired Immune Deficiency Syndromes (1999)

    doi: 10.1097/QAI.0000000000002565

    Comparison of baseline novel miRNA expression in T cells and MDMs. CD4(+) T cells and CD14(+) monocytes were isolated from peripheral blood mononuclear cells of 3 independent healthy donors. A, T cells were stimulated with phytohemagglutinin for 3 days and then cultured for additional 3 days without (Ctrl-Tc) or with 100 ng/mLIL-27 (27-Tc) in the presence of 20 units/mL of IL-2. B, Monocytes were differentiated into macrophages in the presence of 25 ng/mL M-CSF alone (MDMs) or 25 ng/mL M-CSF with 100 ng/mL IL-27 (I-Mac) for 7 days. Total RNA from Ctrl-Tc, 27-Tc, MDMs, and I-Mac were extracted, and the expression of each novel miRNA was quantified by real time qRT-PCR. Gene-specific probes were custom-made by Thermo Fisher Scientific. As an internal control, the small nuclear protein RNU44 probe was used. Base line of the expression of each miRNA was calculated by comparing the Ct values of each miRNA with the Ct value of RNU44 and then subtracting this from 40 (40-delta Ct). 74 Results represent mean ± SE (n = 3) of 3 independent assays.
    Figure Legend Snippet: Comparison of baseline novel miRNA expression in T cells and MDMs. CD4(+) T cells and CD14(+) monocytes were isolated from peripheral blood mononuclear cells of 3 independent healthy donors. A, T cells were stimulated with phytohemagglutinin for 3 days and then cultured for additional 3 days without (Ctrl-Tc) or with 100 ng/mLIL-27 (27-Tc) in the presence of 20 units/mL of IL-2. B, Monocytes were differentiated into macrophages in the presence of 25 ng/mL M-CSF alone (MDMs) or 25 ng/mL M-CSF with 100 ng/mL IL-27 (I-Mac) for 7 days. Total RNA from Ctrl-Tc, 27-Tc, MDMs, and I-Mac were extracted, and the expression of each novel miRNA was quantified by real time qRT-PCR. Gene-specific probes were custom-made by Thermo Fisher Scientific. As an internal control, the small nuclear protein RNU44 probe was used. Base line of the expression of each miRNA was calculated by comparing the Ct values of each miRNA with the Ct value of RNU44 and then subtracting this from 40 (40-delta Ct). 74 Results represent mean ± SE (n = 3) of 3 independent assays.

    Techniques Used: Expressing, Isolation, Cell Culture, Quantitative RT-PCR

    28) Product Images from "PD-L1 up-regulation restrains Th17 cell differentiation in STAT3 loss- and STAT1 gain-of-function patients"

    Article Title: PD-L1 up-regulation restrains Th17 cell differentiation in STAT3 loss- and STAT1 gain-of-function patients

    Journal: The Journal of Experimental Medicine

    doi: 10.1084/jem.20161427

    STAT3 LOF and STAT1 GOF cells have enhanced pSTAT1 that is partially dependent on impaired induction of SOCS3. (A) pSTAT1 (left) and pSTAT3 (right) in PBMCs after stimulation with IL-6, IL-21, or IL-27 for 15 min. (B) Total STAT1 in PBMCS before stimulation (left) and after cytokine stimulation (right) for 15 min. (C and D) SOCS3 and SOCS1 mRNA (C) and SOCS3 protein (D) levels in naive CD4 T cells after stimulation with IL-6, IL-21, or IL-27 for 1 h. (E and F) Naive (CD45RO − ) CD4 + T cells were transfected with SOCS3–GFP or GFP–control or SOCS3 –RNAi or scramble RNAi–control. IL-27 was added 5 h after transfection, and pSTAT1 levels were analyzed 18–24 h later. One-way ANOVA (A–D) and paired and Student’s t tests (E and F) were performed. *, P
    Figure Legend Snippet: STAT3 LOF and STAT1 GOF cells have enhanced pSTAT1 that is partially dependent on impaired induction of SOCS3. (A) pSTAT1 (left) and pSTAT3 (right) in PBMCs after stimulation with IL-6, IL-21, or IL-27 for 15 min. (B) Total STAT1 in PBMCS before stimulation (left) and after cytokine stimulation (right) for 15 min. (C and D) SOCS3 and SOCS1 mRNA (C) and SOCS3 protein (D) levels in naive CD4 T cells after stimulation with IL-6, IL-21, or IL-27 for 1 h. (E and F) Naive (CD45RO − ) CD4 + T cells were transfected with SOCS3–GFP or GFP–control or SOCS3 –RNAi or scramble RNAi–control. IL-27 was added 5 h after transfection, and pSTAT1 levels were analyzed 18–24 h later. One-way ANOVA (A–D) and paired and Student’s t tests (E and F) were performed. *, P

    Techniques Used: Transfection

    High pSTAT1 drives increased PD-L1 expression in STAT3 LOF and STAT1 GOF cells. (A) PD-L1 expression on naive (CD45RO − ) T cells from HCs and patients after 24 h of stimulation with IL-27. Naive (CD45RO − ) CD4 + T cells were transfected with STAT1 RNAi or scramble RNAi (B and C), SOCS3-GFP or GFP-control (D), or SOCS3 RNAi or scramble RNAi (E). (B) STAT1 expression after transfection with STAT1 RNAi. (C–E) PD-L1 expression 41 h after IL-27 stimulation. One-way ANOVA (A) and paired Student’s t tests (C–E) were performed. *, P
    Figure Legend Snippet: High pSTAT1 drives increased PD-L1 expression in STAT3 LOF and STAT1 GOF cells. (A) PD-L1 expression on naive (CD45RO − ) T cells from HCs and patients after 24 h of stimulation with IL-27. Naive (CD45RO − ) CD4 + T cells were transfected with STAT1 RNAi or scramble RNAi (B and C), SOCS3-GFP or GFP-control (D), or SOCS3 RNAi or scramble RNAi (E). (B) STAT1 expression after transfection with STAT1 RNAi. (C–E) PD-L1 expression 41 h after IL-27 stimulation. One-way ANOVA (A) and paired Student’s t tests (C–E) were performed. *, P

    Techniques Used: Expressing, Transfection

    29) Product Images from "HIV-1 infection activates endogenous retroviral promoters regulating antiviral gene expression"

    Article Title: HIV-1 infection activates endogenous retroviral promoters regulating antiviral gene expression

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkaa832

    LTR12C repeats are associated with responsiveness of GBPs to cytokine stimulation. ( A ) HEK293T cells were co-transfected with the indicated firefly luciferase promoter reporter plasmids, a Gaussia luciferase control vector and increasing amounts of the HIV-1 STCO1 clone. Two days post transfection, luciferase activities were determined. Mean values of 4–9 independent experiments ± SEM are shown. ( B ) Primary human CD4 + T cells were stimulated with IFN-α2 (500 U/ml), IFN-γ (200 U/ml) or IL-27 (5 ng/ml) for 3 days or left untreated, and GBP2 and GBP5 expression was analyzed by Western blotting. One representative Western blot is shown. ( C ) Primary human CD4 + T cells were stimulated with IFN-α14 (50 ng/ml) or IL-27 (5 ng/ml) for 3 days or left untreated, and GBP2 and GBP5 mRNA levels were determined by qPCR. Mean values of three independent donors ± SEM are shown. ( D ) Primary human PBMCs were stimulated with IFN-α2 (500 U/ml) IFN-γ (200 U/ml) or IL-27 (50 ng/ml) for 3 days or left untreated, and GBP1, GBP2 and GBP5 expression was analyzed by flow cytometry. Mean values of three independent donors ± SEM are shown. ( E ) Alignment of the LTR12C integration sites upstream of GBP2 and GBP5 in humans (hum), chimpanzees (cpz), bonobos (bon), gorillas (gor), orangutans (oru), African green monkeys (agm), rhesus macaques (rhe) and marmosets (mar). ( F ) Primary rhesus macaque PBMCs were stimulated with IFN-α2 (500 U/ml), IFN-γ (200 U/ml) or IL-27 (50 ng/ml) for 3 days or left untreated, and GBP2 expression was analyzed by flow cytometry. Mean values of four independent donors ± SEM are shown. (* P
    Figure Legend Snippet: LTR12C repeats are associated with responsiveness of GBPs to cytokine stimulation. ( A ) HEK293T cells were co-transfected with the indicated firefly luciferase promoter reporter plasmids, a Gaussia luciferase control vector and increasing amounts of the HIV-1 STCO1 clone. Two days post transfection, luciferase activities were determined. Mean values of 4–9 independent experiments ± SEM are shown. ( B ) Primary human CD4 + T cells were stimulated with IFN-α2 (500 U/ml), IFN-γ (200 U/ml) or IL-27 (5 ng/ml) for 3 days or left untreated, and GBP2 and GBP5 expression was analyzed by Western blotting. One representative Western blot is shown. ( C ) Primary human CD4 + T cells were stimulated with IFN-α14 (50 ng/ml) or IL-27 (5 ng/ml) for 3 days or left untreated, and GBP2 and GBP5 mRNA levels were determined by qPCR. Mean values of three independent donors ± SEM are shown. ( D ) Primary human PBMCs were stimulated with IFN-α2 (500 U/ml) IFN-γ (200 U/ml) or IL-27 (50 ng/ml) for 3 days or left untreated, and GBP1, GBP2 and GBP5 expression was analyzed by flow cytometry. Mean values of three independent donors ± SEM are shown. ( E ) Alignment of the LTR12C integration sites upstream of GBP2 and GBP5 in humans (hum), chimpanzees (cpz), bonobos (bon), gorillas (gor), orangutans (oru), African green monkeys (agm), rhesus macaques (rhe) and marmosets (mar). ( F ) Primary rhesus macaque PBMCs were stimulated with IFN-α2 (500 U/ml), IFN-γ (200 U/ml) or IL-27 (50 ng/ml) for 3 days or left untreated, and GBP2 expression was analyzed by flow cytometry. Mean values of four independent donors ± SEM are shown. (* P

    Techniques Used: Transfection, Luciferase, Plasmid Preparation, Expressing, Western Blot, Real-time Polymerase Chain Reaction, Flow Cytometry

    30) Product Images from "Interleukin-27 inhibition of HIV-1 involves an intermediate induction of type I interferon"

    Article Title: Interleukin-27 inhibition of HIV-1 involves an intermediate induction of type I interferon

    Journal:

    doi: 10.1182/blood-2009-03-211540

    IL-27 inhibits HIV infection in macrophages . (A) Monocyte-derived macrophages were infected with 10 3 TCID 50 /mL BaL for 2 hours. Cells were washed, 10% DMEM added, and IL-27 at 0, 1, 10, and 100 ng/mL added only once after infection. Cells were fed every
    Figure Legend Snippet: IL-27 inhibits HIV infection in macrophages . (A) Monocyte-derived macrophages were infected with 10 3 TCID 50 /mL BaL for 2 hours. Cells were washed, 10% DMEM added, and IL-27 at 0, 1, 10, and 100 ng/mL added only once after infection. Cells were fed every

    Techniques Used: Infection, Derivative Assay

    IFN-α up-regulates IL-27 and IL-27 receptor mRNA expression . (A) Monocyte-derived macrophages (n = 3) were stimulated with IFN-α (10 ng/mL, 4 hours) in parallel with mock-treated cultures, and RNA was extracted and processed for microarray
    Figure Legend Snippet: IFN-α up-regulates IL-27 and IL-27 receptor mRNA expression . (A) Monocyte-derived macrophages (n = 3) were stimulated with IFN-α (10 ng/mL, 4 hours) in parallel with mock-treated cultures, and RNA was extracted and processed for microarray

    Techniques Used: Expressing, Derivative Assay, Microarray

    IL-27 induction of IFN-α and IFN-β supports APOBEC3A/G expression and inhibition of HIV . (A) PBMC were treated with IL-27 at 100 ng/mL for 30 minutes to 24 hours. RNA was reverse transcribed to cDNA, and amplification was performed using
    Figure Legend Snippet: IL-27 induction of IFN-α and IFN-β supports APOBEC3A/G expression and inhibition of HIV . (A) PBMC were treated with IL-27 at 100 ng/mL for 30 minutes to 24 hours. RNA was reverse transcribed to cDNA, and amplification was performed using

    Techniques Used: Expressing, Inhibition, Amplification

    IL-27 enhances macrophage APOBEC RNA expression . Monocyte-derived macrophages were treated 18 to 24 hours with IL-27 at 100 ng/mL or IFN-α at 10 ng/mL. Cells were lysed and processed for RNA following QIAGEN's RNeasy protocol, and gene transcription
    Figure Legend Snippet: IL-27 enhances macrophage APOBEC RNA expression . Monocyte-derived macrophages were treated 18 to 24 hours with IL-27 at 100 ng/mL or IFN-α at 10 ng/mL. Cells were lysed and processed for RNA following QIAGEN's RNeasy protocol, and gene transcription

    Techniques Used: RNA Expression, Derivative Assay

    IL-27 inhibition of T-cell HIV includes an IFN intermediate . (A) IL-27 induction of IFN- α in T cells at 0.5 to 4 hours (4 hours shown; n = 2), without corresponding increase in APOBEC3A until 24 hours but no increase in APOBEC3G (inset; n = 3).
    Figure Legend Snippet: IL-27 inhibition of T-cell HIV includes an IFN intermediate . (A) IL-27 induction of IFN- α in T cells at 0.5 to 4 hours (4 hours shown; n = 2), without corresponding increase in APOBEC3A until 24 hours but no increase in APOBEC3G (inset; n = 3).

    Techniques Used: Inhibition

    Blocking IL-27 signaling reverses inhibition of HIV infection . (A) Inhibition of IL-27 signal transduction by blocking JAK activation interrupts STAT1 and STAT3 phosphorylation. Macrophages were treated with JAK inhibitor at 1 or 10 nM for 1 hour before
    Figure Legend Snippet: Blocking IL-27 signaling reverses inhibition of HIV infection . (A) Inhibition of IL-27 signal transduction by blocking JAK activation interrupts STAT1 and STAT3 phosphorylation. Macrophages were treated with JAK inhibitor at 1 or 10 nM for 1 hour before

    Techniques Used: Blocking Assay, Inhibition, Infection, Transduction, Activation Assay

    31) Product Images from "Autocrine TGF-β1 Maintains the Stability of Foxp3+ Regulatory T Cells via IL-12Rβ2 Downregulation"

    Article Title: Autocrine TGF-β1 Maintains the Stability of Foxp3+ Regulatory T Cells via IL-12Rβ2 Downregulation

    Journal: Biomolecules

    doi: 10.3390/biom10060819

    Ablation of TGF-β1 induces excessive IFN-γ production of Treg cells after IL-12 stimulation. ( A ) Sorted Treg cells from Foxp3 YFP-Cre Tgfb1 fl/+ and Foxp3 YFP-Cre Tgfb1 fl/fl mice were stimulated with anti-CD3ε with (+ group) or without IL-12 (− group). Representative contour plots and quantification of IFN-γ- and IL-17A-expressing cells. ( B ) Sorted Treg cells from Foxp3 YFP-Cre Tgfb1 fl/+ and Foxp3 YFP-Cre Tgfb1 fl/fl mice were stimulated with anti-CD3ε with (+ group) or without IL-27 (− group). Representative contour plots and quantification of IFN-γ- and IL-17A-expressing cells. Data are representative of four ( A , B ) independent experiments, and values are expressed as the mean ± SD ( A , B ). * p ≤ 0.05, ** p ≤ 0.01, and **** p ≤ 0.0001 compared to TGF-β1-sufficient Treg cells based on Student’s t -test. ( C ) Schematic model of the proposed mechanism.
    Figure Legend Snippet: Ablation of TGF-β1 induces excessive IFN-γ production of Treg cells after IL-12 stimulation. ( A ) Sorted Treg cells from Foxp3 YFP-Cre Tgfb1 fl/+ and Foxp3 YFP-Cre Tgfb1 fl/fl mice were stimulated with anti-CD3ε with (+ group) or without IL-12 (− group). Representative contour plots and quantification of IFN-γ- and IL-17A-expressing cells. ( B ) Sorted Treg cells from Foxp3 YFP-Cre Tgfb1 fl/+ and Foxp3 YFP-Cre Tgfb1 fl/fl mice were stimulated with anti-CD3ε with (+ group) or without IL-27 (− group). Representative contour plots and quantification of IFN-γ- and IL-17A-expressing cells. Data are representative of four ( A , B ) independent experiments, and values are expressed as the mean ± SD ( A , B ). * p ≤ 0.05, ** p ≤ 0.01, and **** p ≤ 0.0001 compared to TGF-β1-sufficient Treg cells based on Student’s t -test. ( C ) Schematic model of the proposed mechanism.

    Techniques Used: Mouse Assay, Expressing

    32) Product Images from "Interleukin-27 inhibition of HIV-1 involves an intermediate induction of type I interferon"

    Article Title: Interleukin-27 inhibition of HIV-1 involves an intermediate induction of type I interferon

    Journal:

    doi: 10.1182/blood-2009-03-211540

    IL-27 inhibits HIV infection in macrophages . (A) Monocyte-derived macrophages were infected with 10 3 TCID 50 /mL BaL for 2 hours. Cells were washed, 10% DMEM added, and IL-27 at 0, 1, 10, and 100 ng/mL added only once after infection. Cells were fed every
    Figure Legend Snippet: IL-27 inhibits HIV infection in macrophages . (A) Monocyte-derived macrophages were infected with 10 3 TCID 50 /mL BaL for 2 hours. Cells were washed, 10% DMEM added, and IL-27 at 0, 1, 10, and 100 ng/mL added only once after infection. Cells were fed every

    Techniques Used: Infection, Derivative Assay

    IFN-α up-regulates IL-27 and IL-27 receptor mRNA expression . (A) Monocyte-derived macrophages (n = 3) were stimulated with IFN-α (10 ng/mL, 4 hours) in parallel with mock-treated cultures, and RNA was extracted and processed for microarray
    Figure Legend Snippet: IFN-α up-regulates IL-27 and IL-27 receptor mRNA expression . (A) Monocyte-derived macrophages (n = 3) were stimulated with IFN-α (10 ng/mL, 4 hours) in parallel with mock-treated cultures, and RNA was extracted and processed for microarray

    Techniques Used: Expressing, Derivative Assay, Microarray

    IL-27 induction of IFN-α and IFN-β supports APOBEC3A/G expression and inhibition of HIV . (A) PBMC were treated with IL-27 at 100 ng/mL for 30 minutes to 24 hours. RNA was reverse transcribed to cDNA, and amplification was performed using
    Figure Legend Snippet: IL-27 induction of IFN-α and IFN-β supports APOBEC3A/G expression and inhibition of HIV . (A) PBMC were treated with IL-27 at 100 ng/mL for 30 minutes to 24 hours. RNA was reverse transcribed to cDNA, and amplification was performed using

    Techniques Used: Expressing, Inhibition, Amplification

    IL-27 enhances macrophage APOBEC RNA expression . Monocyte-derived macrophages were treated 18 to 24 hours with IL-27 at 100 ng/mL or IFN-α at 10 ng/mL. Cells were lysed and processed for RNA following QIAGEN's RNeasy protocol, and gene transcription
    Figure Legend Snippet: IL-27 enhances macrophage APOBEC RNA expression . Monocyte-derived macrophages were treated 18 to 24 hours with IL-27 at 100 ng/mL or IFN-α at 10 ng/mL. Cells were lysed and processed for RNA following QIAGEN's RNeasy protocol, and gene transcription

    Techniques Used: RNA Expression, Derivative Assay

    IL-27 inhibition of T-cell HIV includes an IFN intermediate . (A) IL-27 induction of IFN- α in T cells at 0.5 to 4 hours (4 hours shown; n = 2), without corresponding increase in APOBEC3A until 24 hours but no increase in APOBEC3G (inset; n = 3).
    Figure Legend Snippet: IL-27 inhibition of T-cell HIV includes an IFN intermediate . (A) IL-27 induction of IFN- α in T cells at 0.5 to 4 hours (4 hours shown; n = 2), without corresponding increase in APOBEC3A until 24 hours but no increase in APOBEC3G (inset; n = 3).

    Techniques Used: Inhibition

    Blocking IL-27 signaling reverses inhibition of HIV infection . (A) Inhibition of IL-27 signal transduction by blocking JAK activation interrupts STAT1 and STAT3 phosphorylation. Macrophages were treated with JAK inhibitor at 1 or 10 nM for 1 hour before
    Figure Legend Snippet: Blocking IL-27 signaling reverses inhibition of HIV infection . (A) Inhibition of IL-27 signal transduction by blocking JAK activation interrupts STAT1 and STAT3 phosphorylation. Macrophages were treated with JAK inhibitor at 1 or 10 nM for 1 hour before

    Techniques Used: Blocking Assay, Inhibition, Infection, Transduction, Activation Assay

    33) Product Images from "HSP70-Homolog DnaK of Pseudomonas aeruginosa Increases the Production of IL-27 through Expression of EBI3 via TLR4-Dependent NF-κB and TLR4-Independent Akt Signaling"

    Article Title: HSP70-Homolog DnaK of Pseudomonas aeruginosa Increases the Production of IL-27 through Expression of EBI3 via TLR4-Dependent NF-κB and TLR4-Independent Akt Signaling

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms21239194

    IL-27 produced in response to DnaK increases expression of IL10 . dTHP-1 cells were treated with 1 μg/mL rDnaK for 16 h, and the supernatant was collected as conditioned medium (rDnaK-treated Sup). Conditioned media were incubated in the presence and absence of IL-27 antibody for 1 h at room temperature. These conditioned media were transferred to newly seeded dTHP-1 cells, and then the cells were treated with Hk PAK at an MOI of 5 or 10 for 4 h. After treatment, the mRNA levels of IL10 ( A ) and IL1β ( B ) were measured by qRT-PCR. Data are expressed as means ± SD ( n = 3). **, p
    Figure Legend Snippet: IL-27 produced in response to DnaK increases expression of IL10 . dTHP-1 cells were treated with 1 μg/mL rDnaK for 16 h, and the supernatant was collected as conditioned medium (rDnaK-treated Sup). Conditioned media were incubated in the presence and absence of IL-27 antibody for 1 h at room temperature. These conditioned media were transferred to newly seeded dTHP-1 cells, and then the cells were treated with Hk PAK at an MOI of 5 or 10 for 4 h. After treatment, the mRNA levels of IL10 ( A ) and IL1β ( B ) were measured by qRT-PCR. Data are expressed as means ± SD ( n = 3). **, p

    Techniques Used: Produced, Expressing, Incubation, Quantitative RT-PCR

    DnaK-induced EBI3 is involved in formation of IL-27. ( A , B ) dTHP-1 cells were treated with rDnaK at the indicated concentrations for the indicated times. ( C ) dTHP-1 cells were treated with 0.5 μg/mL rDnaK for the indicated times. ( D , E ) dTHP-1 cells were treated with the indicated concentrations of rDnaK for 4 h ( D ) and 0.5 μg/mL rDnaK for the indicated times ( E ). After treatment, protein levels of IL-35 and IL-27 released from dTHP-1 cells were measured by ELISA ( A – C ), and the p28 mRNA level was quantified by qRT-PCR ( D , E ). Data are expressed as means ± SD ( n = 3). ***, p
    Figure Legend Snippet: DnaK-induced EBI3 is involved in formation of IL-27. ( A , B ) dTHP-1 cells were treated with rDnaK at the indicated concentrations for the indicated times. ( C ) dTHP-1 cells were treated with 0.5 μg/mL rDnaK for the indicated times. ( D , E ) dTHP-1 cells were treated with the indicated concentrations of rDnaK for 4 h ( D ) and 0.5 μg/mL rDnaK for the indicated times ( E ). After treatment, protein levels of IL-35 and IL-27 released from dTHP-1 cells were measured by ELISA ( A – C ), and the p28 mRNA level was quantified by qRT-PCR ( D , E ). Data are expressed as means ± SD ( n = 3). ***, p

    Techniques Used: Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR

    34) Product Images from "Immunoregulatory and tissue-residency programs modulated by c-MAF in human TH17 cells"

    Article Title: Immunoregulatory and tissue-residency programs modulated by c-MAF in human TH17 cells

    Journal: Nature immunology

    doi: 10.1038/s41590-018-0200-5

    IL-27 and IL-lβ antagonistically promote the expression of T H 17-IL-10 + and T H 17-IL-10 - associated genes. a,b. T H 17-IL-10 + cells were polyclonally activated with or without IL-1β (10 ng/ml) and the expression of representative immunoregulatory, tissue- residency and pro-inflammatory genes was measured on Day 5 at mRNA level by qPCR, after 2 h re-stimulation with CD3/CD28 antibodies ( a , mean + s.e.m; n > 3), and at protein level by flow cytometry ( b , mean + 95% c.i.; CXCR6, CD25 and CCR7, n = 8; IL-10, IFN-γ and c-MAF, n = 7; CD69 and PD-1, n = 6; CTLA-4, n = 5; BLIMP-1 and FOXP3, n = 4), after 5 h stimulation with PMA+I. c. T H 17-IL-10 - cells were polyclonally activated in presence of IL-27 (25 ng/ml), TGF-β (2 ng/ml) or a combination of the two cytokines and the expression of the indicated proteins was assessed on Day 5 by flow cytometry, after 5 h stimulation with PMA+I (mean + 95% c.i.; n = 6; surface markers TGF-β+IL-27, n = 5). d. T H 17-IL-10 + (n = 4) and T H 17-IL-10 - (n = 6) cells were polyclonally activated in the presence of AHR antagonist (CH-223191, 3 μΜ) or agonist (FICZ, 100 nM) and IL-10 production was measured on Day 5 by flow cytometry, following 5 h PMA+I stimulation (mean + s.e.m.). *P
    Figure Legend Snippet: IL-27 and IL-lβ antagonistically promote the expression of T H 17-IL-10 + and T H 17-IL-10 - associated genes. a,b. T H 17-IL-10 + cells were polyclonally activated with or without IL-1β (10 ng/ml) and the expression of representative immunoregulatory, tissue- residency and pro-inflammatory genes was measured on Day 5 at mRNA level by qPCR, after 2 h re-stimulation with CD3/CD28 antibodies ( a , mean + s.e.m; n > 3), and at protein level by flow cytometry ( b , mean + 95% c.i.; CXCR6, CD25 and CCR7, n = 8; IL-10, IFN-γ and c-MAF, n = 7; CD69 and PD-1, n = 6; CTLA-4, n = 5; BLIMP-1 and FOXP3, n = 4), after 5 h stimulation with PMA+I. c. T H 17-IL-10 - cells were polyclonally activated in presence of IL-27 (25 ng/ml), TGF-β (2 ng/ml) or a combination of the two cytokines and the expression of the indicated proteins was assessed on Day 5 by flow cytometry, after 5 h stimulation with PMA+I (mean + 95% c.i.; n = 6; surface markers TGF-β+IL-27, n = 5). d. T H 17-IL-10 + (n = 4) and T H 17-IL-10 - (n = 6) cells were polyclonally activated in the presence of AHR antagonist (CH-223191, 3 μΜ) or agonist (FICZ, 100 nM) and IL-10 production was measured on Day 5 by flow cytometry, following 5 h PMA+I stimulation (mean + s.e.m.). *P

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Flow Cytometry

    35) Product Images from "The Inhibition of Group II Innate Lymphoid Cell Response by IL-27 in Allergic Rhinitis"

    Article Title: The Inhibition of Group II Innate Lymphoid Cell Response by IL-27 in Allergic Rhinitis

    Journal: Journal of Immunology Research

    doi: 10.1155/2020/6661524

    The IL-27 receptor expression by ILC2 regulated by IL-27. (a, b) The mRNA expression of gp130 and WSX-1 by ILC2 after IL-27 stimulation by polymerase chain reaction. (c) The protein expression of gp130 and WSX-1 by ILC2 after IL-27 stimulation by flow cytometry. Three independent tests were performed for every experiment. ∗ Compared with IL-27 (10 ng/mL), P
    Figure Legend Snippet: The IL-27 receptor expression by ILC2 regulated by IL-27. (a, b) The mRNA expression of gp130 and WSX-1 by ILC2 after IL-27 stimulation by polymerase chain reaction. (c) The protein expression of gp130 and WSX-1 by ILC2 after IL-27 stimulation by flow cytometry. Three independent tests were performed for every experiment. ∗ Compared with IL-27 (10 ng/mL), P

    Techniques Used: Expressing, Polymerase Chain Reaction, Flow Cytometry

    IL-27 inhibited ILC2 cell proliferation and cytokine expression. (a) Proliferation of ILC2 was assessed by tritiated thymidine incorporation under IL-27 stimulation. (b, c) The mRNA expression of GATA-3 and ROR α by ILC2 detected by PCR. (d, e) Type II cytokine protein expression by ILCA2 measured by ELISA. Three independent tests were performed for every experiment. ∗ Compared with PBS, P
    Figure Legend Snippet: IL-27 inhibited ILC2 cell proliferation and cytokine expression. (a) Proliferation of ILC2 was assessed by tritiated thymidine incorporation under IL-27 stimulation. (b, c) The mRNA expression of GATA-3 and ROR α by ILC2 detected by PCR. (d, e) Type II cytokine protein expression by ILCA2 measured by ELISA. Three independent tests were performed for every experiment. ∗ Compared with PBS, P

    Techniques Used: Expressing, Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

    IL-27 inhibits allergic responses in mouse model. (a) Levels of Der p1-specific IgE by enzyme-linked immunosorbent assay after IL-27 stimulation. (b, c) The proportions of nasal ILC2 in total cells of inferior turbinate tissue after IL-27 treatment by flow cytometry. (d–f) The proportions of nasal IL-5+ILC2 and IL-13+ILC2 in total cells of inferior turbinate tissue. After IL-27 treatment by flow cytometry. Ten mice were allocated into every group. Three independent tests were performed for every experiment. ∗ Compared with PBS, P
    Figure Legend Snippet: IL-27 inhibits allergic responses in mouse model. (a) Levels of Der p1-specific IgE by enzyme-linked immunosorbent assay after IL-27 stimulation. (b, c) The proportions of nasal ILC2 in total cells of inferior turbinate tissue after IL-27 treatment by flow cytometry. (d–f) The proportions of nasal IL-5+ILC2 and IL-13+ILC2 in total cells of inferior turbinate tissue. After IL-27 treatment by flow cytometry. Ten mice were allocated into every group. Three independent tests were performed for every experiment. ∗ Compared with PBS, P

    Techniques Used: Enzyme-linked Immunosorbent Assay, Flow Cytometry, Mouse Assay

    The serum expression of IL-27 and the proportion of ILC2 in PBMCs between AR and controls. (a, b) Expression of serum IL-27 mRNA and protein levels between AR and controls by polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. (c) Frequency of ILC2 in PBMCs between controls and AR as well as isotype and FMO controls by flow cytometry. AR: allergic rhinitis; FMO: fluorescence minus one; HC: healthy control; ILC2: group II innate lymphoid cells; PBMC: peripheral blood mononuclear cells.
    Figure Legend Snippet: The serum expression of IL-27 and the proportion of ILC2 in PBMCs between AR and controls. (a, b) Expression of serum IL-27 mRNA and protein levels between AR and controls by polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. (c) Frequency of ILC2 in PBMCs between controls and AR as well as isotype and FMO controls by flow cytometry. AR: allergic rhinitis; FMO: fluorescence minus one; HC: healthy control; ILC2: group II innate lymphoid cells; PBMC: peripheral blood mononuclear cells.

    Techniques Used: Expressing, Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Fluorescence

    Correlation between of IL-27 protein expression, the proportion of ILC2 in PBMCs, and TNSS score in AR. (a, b) Correlation between IL-27 protein levels and ILC2 frequency and TNSS score. (c) Correlation between ILC2 frequency and TNSS score. AR: allergic rhinitis; ILC2: group II innate lymphoid cells; PBMC: peripheral blood mononuclear cells; TNSS: total nasal symptom score.
    Figure Legend Snippet: Correlation between of IL-27 protein expression, the proportion of ILC2 in PBMCs, and TNSS score in AR. (a, b) Correlation between IL-27 protein levels and ILC2 frequency and TNSS score. (c) Correlation between ILC2 frequency and TNSS score. AR: allergic rhinitis; ILC2: group II innate lymphoid cells; PBMC: peripheral blood mononuclear cells; TNSS: total nasal symptom score.

    Techniques Used: Expressing

    Related Articles

    Recombinant:

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    Article Snippet: All RT-PCR primers and reagents were obtained from Applied Biosystems Life Technologies, Carlsbad California, USA. .. Recombinant human IL-27, IL-12 and IL-15 were obtained from R & D systems, Minneapolis, USA. .. Purification of human NK cell subsets PBMCs were obtained by Ficoll density gradient.

    Article Title: Persistent loss of IL-27 responsiveness in CD8+ memory T cells abrogates IL-10 expression in a recall response
    Article Snippet: Cryopreserved human PBMCs were purchased from Cellular Technology Ltd. .. Cells were labeled with a Pro5 Recombinant Murine MHC Pentamer specific for the influenza A M1 epitope (Proimmune), stimulated for 15 min at 37 °C with 20 ng/mL recombinant human IL-27 (R & D Systems), and stained for pSTAT1 and pSTAT3 as above. .. Surface antigens were assessed using fluorochrome-labeled antibodies against CD8 (clone RPA-T8), CD19 (HIB19), CD27 (O323), and CD45RA (HI100) (eBioscience).

    Article Title: IL-27 Promotes Proliferation of Human Leukemic Cell Lines Through the MAPK/ERK Signaling Pathway and Suppresses Sensitivity to Chemotherapeutic Drugs
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    Article Title: Proteomic analysis uncovers common effects of IFN-γ and IL-27 on the HLA class I antigen presentation machinery in human cancer cells
    Article Snippet: .. The day after, the culture medium was replaced with fresh medium with or without recombinant human IFN-γ (1,000 IU/ml, PeproTech, 300-02) or IL-27 (100 ng/ml, R & D System, 2526-IL-010). .. The culture was then carried on for 48 hours and cells, detached by 2 mM EDTA in PBS, were washed and analyzed by flow cytometry.

    Article Title: IL-27 and IL-21 are Associated with T Cell IL-10 Responses in Human Visceral Leishmaniasis
    Article Snippet: Whole blood cells were cultured in the absence of antigen or stimulated with L. donovani soluble antigen (SLA, 10µg/ml) prepared from L. donovani stationary-phage promastigotes (prepared as described previously, ( )). .. Where indicated, recombinant human IL-27 (100ng/ml) (R & D Systems) or human IL-21 (25ng/ml) (PeproTech) alone or in combination, were added to the whole blood cultures. ..

    Article Title: Production of interleukin 27 by human neutrophils regulates their function in response to bacterial infection
    Article Snippet: Then neutrophils were isolated as previously described [ ] and CD14+ , CD4+ and CD8+ cells were separated by using immune-magnetic bead sorting (Miltenyi Biotec), according to the manufacturer’s instruction. .. Whole blood samples from healthy subjects were stimulated in vitro with B. pseudomallei at MOI of 10 or with 100 ng/ml lipopolysaccharide of Escherichia coli ( E. coli LPS) (Sigma Aldrich) in the presence of recombinant human IL-27 (rIL-27, R & D Systems) at 0, 10, 30 or 100 ng/ml for 30 min at 37°C; then analyzed for oxidative burst by flow cytometry ( ) as previously described by Chanchamroen et al. [ ]. .. Purified neutrophils were treated with 0, 30, 100 or 300 ng/ml of rIL-27 before co-culturing with B. pseudomallei at a MOI of 10 at 37°C for 30 min.

    Article Title: Anti-Inflammatory Effects of IL-27 in Zymosan-Induced Peritonitis: Inhibition of Neutrophil Recruitment Partially Explained by Impaired Mobilization from Bone Marrow and Reduced Chemokine Levels
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    Article Title: IL-27 inhibits human osteoclastogenesis by abrogating RANKL-mediated induction of NFATc1 and suppressing proximal RANK signaling
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    Labeling:

    Article Title: Persistent loss of IL-27 responsiveness in CD8+ memory T cells abrogates IL-10 expression in a recall response
    Article Snippet: Cryopreserved human PBMCs were purchased from Cellular Technology Ltd. .. Cells were labeled with a Pro5 Recombinant Murine MHC Pentamer specific for the influenza A M1 epitope (Proimmune), stimulated for 15 min at 37 °C with 20 ng/mL recombinant human IL-27 (R & D Systems), and stained for pSTAT1 and pSTAT3 as above. .. Surface antigens were assessed using fluorochrome-labeled antibodies against CD8 (clone RPA-T8), CD19 (HIB19), CD27 (O323), and CD45RA (HI100) (eBioscience).

    Staining:

    Article Title: Persistent loss of IL-27 responsiveness in CD8+ memory T cells abrogates IL-10 expression in a recall response
    Article Snippet: Cryopreserved human PBMCs were purchased from Cellular Technology Ltd. .. Cells were labeled with a Pro5 Recombinant Murine MHC Pentamer specific for the influenza A M1 epitope (Proimmune), stimulated for 15 min at 37 °C with 20 ng/mL recombinant human IL-27 (R & D Systems), and stained for pSTAT1 and pSTAT3 as above. .. Surface antigens were assessed using fluorochrome-labeled antibodies against CD8 (clone RPA-T8), CD19 (HIB19), CD27 (O323), and CD45RA (HI100) (eBioscience).

    Functional Assay:

    Article Title: IL-27 Promotes Proliferation of Human Leukemic Cell Lines Through the MAPK/ERK Signaling Pathway and Suppresses Sensitivity to Chemotherapeutic Drugs
    Article Snippet: .. Reagents Recombinant human IL-27, recombinant human TNF-α, recombinant human IL27Rα-Fc chimera, recombinant human gp130-Fc chimera, and functional blocking antibody against human gp130 were purchased from R & D Systems (Abingdon, United Kingdom). .. Cell culture media, cell dissociation solution, phorbol 12-myristate 13-acetate (PMA), U0126, wortmannin, cytarabine, and daunorubicin were purchased from Sigma-Aldrich (Dorset, United Kingdom).

    Blocking Assay:

    Article Title: IL-27 Promotes Proliferation of Human Leukemic Cell Lines Through the MAPK/ERK Signaling Pathway and Suppresses Sensitivity to Chemotherapeutic Drugs
    Article Snippet: .. Reagents Recombinant human IL-27, recombinant human TNF-α, recombinant human IL27Rα-Fc chimera, recombinant human gp130-Fc chimera, and functional blocking antibody against human gp130 were purchased from R & D Systems (Abingdon, United Kingdom). .. Cell culture media, cell dissociation solution, phorbol 12-myristate 13-acetate (PMA), U0126, wortmannin, cytarabine, and daunorubicin were purchased from Sigma-Aldrich (Dorset, United Kingdom).

    In Vitro:

    Article Title: Production of interleukin 27 by human neutrophils regulates their function in response to bacterial infection
    Article Snippet: Then neutrophils were isolated as previously described [ ] and CD14+ , CD4+ and CD8+ cells were separated by using immune-magnetic bead sorting (Miltenyi Biotec), according to the manufacturer’s instruction. .. Whole blood samples from healthy subjects were stimulated in vitro with B. pseudomallei at MOI of 10 or with 100 ng/ml lipopolysaccharide of Escherichia coli ( E. coli LPS) (Sigma Aldrich) in the presence of recombinant human IL-27 (rIL-27, R & D Systems) at 0, 10, 30 or 100 ng/ml for 30 min at 37°C; then analyzed for oxidative burst by flow cytometry ( ) as previously described by Chanchamroen et al. [ ]. .. Purified neutrophils were treated with 0, 30, 100 or 300 ng/ml of rIL-27 before co-culturing with B. pseudomallei at a MOI of 10 at 37°C for 30 min.

    Flow Cytometry:

    Article Title: Production of interleukin 27 by human neutrophils regulates their function in response to bacterial infection
    Article Snippet: Then neutrophils were isolated as previously described [ ] and CD14+ , CD4+ and CD8+ cells were separated by using immune-magnetic bead sorting (Miltenyi Biotec), according to the manufacturer’s instruction. .. Whole blood samples from healthy subjects were stimulated in vitro with B. pseudomallei at MOI of 10 or with 100 ng/ml lipopolysaccharide of Escherichia coli ( E. coli LPS) (Sigma Aldrich) in the presence of recombinant human IL-27 (rIL-27, R & D Systems) at 0, 10, 30 or 100 ng/ml for 30 min at 37°C; then analyzed for oxidative burst by flow cytometry ( ) as previously described by Chanchamroen et al. [ ]. .. Purified neutrophils were treated with 0, 30, 100 or 300 ng/ml of rIL-27 before co-culturing with B. pseudomallei at a MOI of 10 at 37°C for 30 min.

    Cytometry:

    Article Title: Production of interleukin 27 by human neutrophils regulates their function in response to bacterial infection
    Article Snippet: Then neutrophils were isolated as previously described [ ] and CD14+ , CD4+ and CD8+ cells were separated by using immune-magnetic bead sorting (Miltenyi Biotec), according to the manufacturer’s instruction. .. Whole blood samples from healthy subjects were stimulated in vitro with B. pseudomallei at MOI of 10 or with 100 ng/ml lipopolysaccharide of Escherichia coli ( E. coli LPS) (Sigma Aldrich) in the presence of recombinant human IL-27 (rIL-27, R & D Systems) at 0, 10, 30 or 100 ng/ml for 30 min at 37°C; then analyzed for oxidative burst by flow cytometry ( ) as previously described by Chanchamroen et al. [ ]. .. Purified neutrophils were treated with 0, 30, 100 or 300 ng/ml of rIL-27 before co-culturing with B. pseudomallei at a MOI of 10 at 37°C for 30 min.

    Injection:

    Article Title: Anti-Inflammatory Effects of IL-27 in Zymosan-Induced Peritonitis: Inhibition of Neutrophil Recruitment Partially Explained by Impaired Mobilization from Bone Marrow and Reduced Chemokine Levels
    Article Snippet: Peritonitis induction and application of IL-27 Zymosan A (Sigma Z-4250, Saccharomyces cerevisiae) powder was suspended in PBS (1 mg/ml), sonicated and 1ml was injected intraperitoneally (i.p.) after the mice were anesthetized by isoflurane (1.5–2% in 2:3 oxygen/nitric oxide, inhaled). .. 200 ng recombinant IL-27 (R & D Systems) was injected i.p. 12 h prior to the administration of zymosan for the preIL-27 group and simultaneously for the IL-27 group. ..

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    R&D Systems cytokine cocktail mix
    Multiparametric functional assessment of EBV- and CMV-specific CD8 + T cells in SLE patients. ( A ) Representative cytofluorometric detection (left) and functional analysis (right) of CD8 + T cells specific for one of the lytic EBV antigens tested (BZLF1) in a healthy control (upper panel) and in an inactive SLE patient (lover panel) post peptide antigen stimulation of PBMC. Lytic EBV and CMV antigen-specific cells were detected with peptide/MHC tetramer and anti-CD8 antibody (red box) and simultaneously analyzed for intra-cellular IFN-γ, TNF-α, IL-2 and MIP-1β content. <t>Cytokine/chemokine</t> gates were positioned according to control stains of non-stimulated virus-specific T cells. (B) Magnitude and (C) functionality of EBV- (upper panel) and CMV-specific (lower panel) responses in healthy controls (H, n = 26 and 15, respectively), inactive (i, n = 19 and 10) and active (a, n = 27 and 11) SLE patients. (D) EBV-specific T cells (upper panel) are strikingly less polyfunctional in inactive (iSLE) and active (aSLE) SLE patients compared to controls (healthy), while polyfunctionality of CMV-specific responses (lower panel) is preserved. Pie representations of virus-specific CD8 + T cells represent the fraction of individual cells secreting none (0) or any (1, 2, 3 or 4) of the four cytokines IFN-γ, TNF-α, IL-2 and MIP-1β (color coded as indicated). E.g. the red pie slice indicates the proportion of cells producing four cytokines (IFN-γ, TNF-α, IL-2 and MIP-1β). P -values monitoring differences between healthy donors and SLE patients are calculated using a non-parametric Mann-Whitney test and pie comparison statistics of the Spice software.
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    <t>IL-27</t> inhibits HIV infection in macrophages . (A) Monocyte-derived macrophages were infected with 10 3 TCID 50 /mL BaL for 2 hours. Cells were washed, 10% DMEM added, and IL-27 at 0, 1, 10, and 100 ng/mL added only once after infection. Cells were fed every
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    Label-free quantified proteins represented as relative protein levels. The calprotectin significant modulatory effects on mutual proteins in HUVECs stimulated with <t>IL27</t> (30 ng/mL) ± calprotectin (1 μ g/mL) are shown for 6, 12, and 24 h ( n = 9). Significance is indicated by q -value: ∗ q
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    Multiparametric functional assessment of EBV- and CMV-specific CD8 + T cells in SLE patients. ( A ) Representative cytofluorometric detection (left) and functional analysis (right) of CD8 + T cells specific for one of the lytic EBV antigens tested (BZLF1) in a healthy control (upper panel) and in an inactive SLE patient (lover panel) post peptide antigen stimulation of PBMC. Lytic EBV and CMV antigen-specific cells were detected with peptide/MHC tetramer and anti-CD8 antibody (red box) and simultaneously analyzed for intra-cellular IFN-γ, TNF-α, IL-2 and MIP-1β content. Cytokine/chemokine gates were positioned according to control stains of non-stimulated virus-specific T cells. (B) Magnitude and (C) functionality of EBV- (upper panel) and CMV-specific (lower panel) responses in healthy controls (H, n = 26 and 15, respectively), inactive (i, n = 19 and 10) and active (a, n = 27 and 11) SLE patients. (D) EBV-specific T cells (upper panel) are strikingly less polyfunctional in inactive (iSLE) and active (aSLE) SLE patients compared to controls (healthy), while polyfunctionality of CMV-specific responses (lower panel) is preserved. Pie representations of virus-specific CD8 + T cells represent the fraction of individual cells secreting none (0) or any (1, 2, 3 or 4) of the four cytokines IFN-γ, TNF-α, IL-2 and MIP-1β (color coded as indicated). E.g. the red pie slice indicates the proportion of cells producing four cytokines (IFN-γ, TNF-α, IL-2 and MIP-1β). P -values monitoring differences between healthy donors and SLE patients are calculated using a non-parametric Mann-Whitney test and pie comparison statistics of the Spice software.

    Journal: PLoS Pathogens

    Article Title: Exhausted Cytotoxic Control of Epstein-Barr Virus in Human Lupus

    doi: 10.1371/journal.ppat.1002328

    Figure Lengend Snippet: Multiparametric functional assessment of EBV- and CMV-specific CD8 + T cells in SLE patients. ( A ) Representative cytofluorometric detection (left) and functional analysis (right) of CD8 + T cells specific for one of the lytic EBV antigens tested (BZLF1) in a healthy control (upper panel) and in an inactive SLE patient (lover panel) post peptide antigen stimulation of PBMC. Lytic EBV and CMV antigen-specific cells were detected with peptide/MHC tetramer and anti-CD8 antibody (red box) and simultaneously analyzed for intra-cellular IFN-γ, TNF-α, IL-2 and MIP-1β content. Cytokine/chemokine gates were positioned according to control stains of non-stimulated virus-specific T cells. (B) Magnitude and (C) functionality of EBV- (upper panel) and CMV-specific (lower panel) responses in healthy controls (H, n = 26 and 15, respectively), inactive (i, n = 19 and 10) and active (a, n = 27 and 11) SLE patients. (D) EBV-specific T cells (upper panel) are strikingly less polyfunctional in inactive (iSLE) and active (aSLE) SLE patients compared to controls (healthy), while polyfunctionality of CMV-specific responses (lower panel) is preserved. Pie representations of virus-specific CD8 + T cells represent the fraction of individual cells secreting none (0) or any (1, 2, 3 or 4) of the four cytokines IFN-γ, TNF-α, IL-2 and MIP-1β (color coded as indicated). E.g. the red pie slice indicates the proportion of cells producing four cytokines (IFN-γ, TNF-α, IL-2 and MIP-1β). P -values monitoring differences between healthy donors and SLE patients are calculated using a non-parametric Mann-Whitney test and pie comparison statistics of the Spice software.

    Article Snippet: Blockade of PD-1 signal pathway PBMCs were cultured for 10 days at 37°C 5% C02, in RPMI supplemented with 5% human serum and a cytokine cocktail mix (20 ng/ml of IL-7 and 20 ng/ml IL-2 (R & D Systems, Minneapolis, MN)).

    Techniques: Functional Assay, MANN-WHITNEY, Software

    IL-27 inhibits HIV infection in macrophages . (A) Monocyte-derived macrophages were infected with 10 3 TCID 50 /mL BaL for 2 hours. Cells were washed, 10% DMEM added, and IL-27 at 0, 1, 10, and 100 ng/mL added only once after infection. Cells were fed every

    Journal:

    Article Title: Interleukin-27 inhibition of HIV-1 involves an intermediate induction of type I interferon

    doi: 10.1182/blood-2009-03-211540

    Figure Lengend Snippet: IL-27 inhibits HIV infection in macrophages . (A) Monocyte-derived macrophages were infected with 10 3 TCID 50 /mL BaL for 2 hours. Cells were washed, 10% DMEM added, and IL-27 at 0, 1, 10, and 100 ng/mL added only once after infection. Cells were fed every

    Article Snippet: Critically, the ability of IL-27 to enforce an HIV blockade is also impaired in the presence of the IFNAR antibody.

    Techniques: Infection, Derivative Assay

    IFN-α up-regulates IL-27 and IL-27 receptor mRNA expression . (A) Monocyte-derived macrophages (n = 3) were stimulated with IFN-α (10 ng/mL, 4 hours) in parallel with mock-treated cultures, and RNA was extracted and processed for microarray

    Journal:

    Article Title: Interleukin-27 inhibition of HIV-1 involves an intermediate induction of type I interferon

    doi: 10.1182/blood-2009-03-211540

    Figure Lengend Snippet: IFN-α up-regulates IL-27 and IL-27 receptor mRNA expression . (A) Monocyte-derived macrophages (n = 3) were stimulated with IFN-α (10 ng/mL, 4 hours) in parallel with mock-treated cultures, and RNA was extracted and processed for microarray

    Article Snippet: Critically, the ability of IL-27 to enforce an HIV blockade is also impaired in the presence of the IFNAR antibody.

    Techniques: Expressing, Derivative Assay, Microarray

    IL-27 induction of IFN-α and IFN-β supports APOBEC3A/G expression and inhibition of HIV . (A) PBMC were treated with IL-27 at 100 ng/mL for 30 minutes to 24 hours. RNA was reverse transcribed to cDNA, and amplification was performed using

    Journal:

    Article Title: Interleukin-27 inhibition of HIV-1 involves an intermediate induction of type I interferon

    doi: 10.1182/blood-2009-03-211540

    Figure Lengend Snippet: IL-27 induction of IFN-α and IFN-β supports APOBEC3A/G expression and inhibition of HIV . (A) PBMC were treated with IL-27 at 100 ng/mL for 30 minutes to 24 hours. RNA was reverse transcribed to cDNA, and amplification was performed using

    Article Snippet: Critically, the ability of IL-27 to enforce an HIV blockade is also impaired in the presence of the IFNAR antibody.

    Techniques: Expressing, Inhibition, Amplification

    IL-27 enhances macrophage APOBEC RNA expression . Monocyte-derived macrophages were treated 18 to 24 hours with IL-27 at 100 ng/mL or IFN-α at 10 ng/mL. Cells were lysed and processed for RNA following QIAGEN's RNeasy protocol, and gene transcription

    Journal:

    Article Title: Interleukin-27 inhibition of HIV-1 involves an intermediate induction of type I interferon

    doi: 10.1182/blood-2009-03-211540

    Figure Lengend Snippet: IL-27 enhances macrophage APOBEC RNA expression . Monocyte-derived macrophages were treated 18 to 24 hours with IL-27 at 100 ng/mL or IFN-α at 10 ng/mL. Cells were lysed and processed for RNA following QIAGEN's RNeasy protocol, and gene transcription

    Article Snippet: Critically, the ability of IL-27 to enforce an HIV blockade is also impaired in the presence of the IFNAR antibody.

    Techniques: RNA Expression, Derivative Assay

    IL-27 inhibition of T-cell HIV includes an IFN intermediate . (A) IL-27 induction of IFN- α in T cells at 0.5 to 4 hours (4 hours shown; n = 2), without corresponding increase in APOBEC3A until 24 hours but no increase in APOBEC3G (inset; n = 3).

    Journal:

    Article Title: Interleukin-27 inhibition of HIV-1 involves an intermediate induction of type I interferon

    doi: 10.1182/blood-2009-03-211540

    Figure Lengend Snippet: IL-27 inhibition of T-cell HIV includes an IFN intermediate . (A) IL-27 induction of IFN- α in T cells at 0.5 to 4 hours (4 hours shown; n = 2), without corresponding increase in APOBEC3A until 24 hours but no increase in APOBEC3G (inset; n = 3).

    Article Snippet: Critically, the ability of IL-27 to enforce an HIV blockade is also impaired in the presence of the IFNAR antibody.

    Techniques: Inhibition

    Blocking IL-27 signaling reverses inhibition of HIV infection . (A) Inhibition of IL-27 signal transduction by blocking JAK activation interrupts STAT1 and STAT3 phosphorylation. Macrophages were treated with JAK inhibitor at 1 or 10 nM for 1 hour before

    Journal:

    Article Title: Interleukin-27 inhibition of HIV-1 involves an intermediate induction of type I interferon

    doi: 10.1182/blood-2009-03-211540

    Figure Lengend Snippet: Blocking IL-27 signaling reverses inhibition of HIV infection . (A) Inhibition of IL-27 signal transduction by blocking JAK activation interrupts STAT1 and STAT3 phosphorylation. Macrophages were treated with JAK inhibitor at 1 or 10 nM for 1 hour before

    Article Snippet: Critically, the ability of IL-27 to enforce an HIV blockade is also impaired in the presence of the IFNAR antibody.

    Techniques: Blocking Assay, Inhibition, Infection, Transduction, Activation Assay

    eNOS expressions in the urinary bladders of E. coli endotoxin-treated mice. Urinary bladders were isolated from mice at 1 h (A) or 6 h (B) after the intraperitoneal injection of E. coli endotoxin (LPS) in the presence or absence of anti-IL-6 antibody

    Journal:

    Article Title: Uropathogenic Escherichia coli-Induced Inflammation Alters Mouse Urinary Bladder Contraction via an Interleukin-6-Activated Inducible Nitric Oxide Synthase-Related Pathway ▿

    doi: 10.1128/IAI.00013-09

    Figure Lengend Snippet: eNOS expressions in the urinary bladders of E. coli endotoxin-treated mice. Urinary bladders were isolated from mice at 1 h (A) or 6 h (B) after the intraperitoneal injection of E. coli endotoxin (LPS) in the presence or absence of anti-IL-6 antibody

    Article Snippet: In some experiments, mice were intraperitoneally injected or intravesically instilled with anti-mouse IL-6 neutralizing antibody (anti-IL-6Ab [R & D Systems]; 1 μg/kg or 0.1 μg in 100 μl of sterile PBS), normal goat immunoglobulin G (IgG; a negative control for IL-6Ab [R & D Systems]; 1 μg/kg or 0.1 μg in 100 μl of sterile PBS), N G -nitro- l -arginine methyl ester (L-NAME; an NOS inhibitor; 10 mg/kg), aminoguanidine (an iNOS inhibitor; 50 mg/kg or 10 mg in 100 μl of sterile PBS), l - N 6 -(1-iminoethyl)lysine (L-NIL; an iNOS inhibitor; 10 mg/kg), or 1400W ( N -[(3-aminomethyl)benzyl]acetamidine [Sigma]; a potent selective iNOS inhibitor, 5 mg/kg), 30 min after LPS or J96 treatment.

    Techniques: Mouse Assay, Isolation, Injection

    Levels of IL-6 in the urinary bladders of E. coli - or endotoxin-treated mice. (A) Urinary bladders were isolated from mice 1, 6, and 24 h after intraperitoneal injection of E. coli endotoxin (LPS). (B) Bladders were isolated from mice 6 h after the intravesical

    Journal:

    Article Title: Uropathogenic Escherichia coli-Induced Inflammation Alters Mouse Urinary Bladder Contraction via an Interleukin-6-Activated Inducible Nitric Oxide Synthase-Related Pathway ▿

    doi: 10.1128/IAI.00013-09

    Figure Lengend Snippet: Levels of IL-6 in the urinary bladders of E. coli - or endotoxin-treated mice. (A) Urinary bladders were isolated from mice 1, 6, and 24 h after intraperitoneal injection of E. coli endotoxin (LPS). (B) Bladders were isolated from mice 6 h after the intravesical

    Article Snippet: In some experiments, mice were intraperitoneally injected or intravesically instilled with anti-mouse IL-6 neutralizing antibody (anti-IL-6Ab [R & D Systems]; 1 μg/kg or 0.1 μg in 100 μl of sterile PBS), normal goat immunoglobulin G (IgG; a negative control for IL-6Ab [R & D Systems]; 1 μg/kg or 0.1 μg in 100 μl of sterile PBS), N G -nitro- l -arginine methyl ester (L-NAME; an NOS inhibitor; 10 mg/kg), aminoguanidine (an iNOS inhibitor; 50 mg/kg or 10 mg in 100 μl of sterile PBS), l - N 6 -(1-iminoethyl)lysine (L-NIL; an iNOS inhibitor; 10 mg/kg), or 1400W ( N -[(3-aminomethyl)benzyl]acetamidine [Sigma]; a potent selective iNOS inhibitor, 5 mg/kg), 30 min after LPS or J96 treatment.

    Techniques: Mouse Assay, Isolation, Injection

    Induction of iNOS and IL-6 mRNA expression by E. coli endotoxin in the mouse urinary bladder. Urinary bladders were isolated from mice 3 to 24 h after intraperitoneal injection of E. coli endotoxin (LPS) in the presence or absence of iNOS inhibitors aminoguanidine

    Journal:

    Article Title: Uropathogenic Escherichia coli-Induced Inflammation Alters Mouse Urinary Bladder Contraction via an Interleukin-6-Activated Inducible Nitric Oxide Synthase-Related Pathway ▿

    doi: 10.1128/IAI.00013-09

    Figure Lengend Snippet: Induction of iNOS and IL-6 mRNA expression by E. coli endotoxin in the mouse urinary bladder. Urinary bladders were isolated from mice 3 to 24 h after intraperitoneal injection of E. coli endotoxin (LPS) in the presence or absence of iNOS inhibitors aminoguanidine

    Article Snippet: In some experiments, mice were intraperitoneally injected or intravesically instilled with anti-mouse IL-6 neutralizing antibody (anti-IL-6Ab [R & D Systems]; 1 μg/kg or 0.1 μg in 100 μl of sterile PBS), normal goat immunoglobulin G (IgG; a negative control for IL-6Ab [R & D Systems]; 1 μg/kg or 0.1 μg in 100 μl of sterile PBS), N G -nitro- l -arginine methyl ester (L-NAME; an NOS inhibitor; 10 mg/kg), aminoguanidine (an iNOS inhibitor; 50 mg/kg or 10 mg in 100 μl of sterile PBS), l - N 6 -(1-iminoethyl)lysine (L-NIL; an iNOS inhibitor; 10 mg/kg), or 1400W ( N -[(3-aminomethyl)benzyl]acetamidine [Sigma]; a potent selective iNOS inhibitor, 5 mg/kg), 30 min after LPS or J96 treatment.

    Techniques: Expressing, Isolation, Mouse Assay, Injection

    Label-free quantified proteins represented as relative protein levels. The calprotectin significant modulatory effects on mutual proteins in HUVECs stimulated with IL27 (30 ng/mL) ± calprotectin (1 μ g/mL) are shown for 6, 12, and 24 h ( n = 9). Significance is indicated by q -value: ∗ q

    Journal: Mediators of Inflammation

    Article Title: Role of Calprotectin as a Modulator of the IL27-Mediated Proinflammatory Effect on Endothelial Cells

    doi: 10.1155/2015/737310

    Figure Lengend Snippet: Label-free quantified proteins represented as relative protein levels. The calprotectin significant modulatory effects on mutual proteins in HUVECs stimulated with IL27 (30 ng/mL) ± calprotectin (1 μ g/mL) are shown for 6, 12, and 24 h ( n = 9). Significance is indicated by q -value: ∗ q

    Article Snippet: This scavenger function does not directly imply a prevention of receptor binding and subsequent activation of the signaling pathway, as it depends on the availability of free binding sites of IL27.

    Techniques:

    Top 20 biological functions from a comparative gene enrichment analysis of IL27 and calprotectin-mediated gene expression. The biological function gene enrichment analysis was carried out by IPA and represents the z -score (activation ≥ 2).

    Journal: Mediators of Inflammation

    Article Title: Role of Calprotectin as a Modulator of the IL27-Mediated Proinflammatory Effect on Endothelial Cells

    doi: 10.1155/2015/737310

    Figure Lengend Snippet: Top 20 biological functions from a comparative gene enrichment analysis of IL27 and calprotectin-mediated gene expression. The biological function gene enrichment analysis was carried out by IPA and represents the z -score (activation ≥ 2).

    Article Snippet: This scavenger function does not directly imply a prevention of receptor binding and subsequent activation of the signaling pathway, as it depends on the availability of free binding sites of IL27.

    Techniques: Expressing, Indirect Immunoperoxidase Assay, Activation Assay

    Effects of IL27, calprotectin, and IL27 + calprotectin cotreatment on STAT1/3 phosphorylation. Relative protein levels of Tyr701-phosphorylated pSTAT1 (a) and Tyr705-pSTAT3 (b) in IL27 (30 ng/mL) ± calprotectin (1 μ g/mL)-stimulated HUVECs for 3, 6, 12, and 24 h performed by western blot. Values are represented as mean ± SEM. Normalization was performed against tubulin. Presented statistical significances are between IL27 and IL27 + calprotectin and the indicated p values correspond to ∗∗∗ p

    Journal: Mediators of Inflammation

    Article Title: Role of Calprotectin as a Modulator of the IL27-Mediated Proinflammatory Effect on Endothelial Cells

    doi: 10.1155/2015/737310

    Figure Lengend Snippet: Effects of IL27, calprotectin, and IL27 + calprotectin cotreatment on STAT1/3 phosphorylation. Relative protein levels of Tyr701-phosphorylated pSTAT1 (a) and Tyr705-pSTAT3 (b) in IL27 (30 ng/mL) ± calprotectin (1 μ g/mL)-stimulated HUVECs for 3, 6, 12, and 24 h performed by western blot. Values are represented as mean ± SEM. Normalization was performed against tubulin. Presented statistical significances are between IL27 and IL27 + calprotectin and the indicated p values correspond to ∗∗∗ p

    Article Snippet: This scavenger function does not directly imply a prevention of receptor binding and subsequent activation of the signaling pathway, as it depends on the availability of free binding sites of IL27.

    Techniques: Western Blot

    Hypothetical model of the calprotectin modulatory effects on IL27-mediated gene and protein expression based on our experimental results and literature data. GBP1, guanylate binding protein 1; NID1, nidogen-1; PECAM1, platelet endothelial cell adhesion molecule; TPM1, tropomyosin 1.

    Journal: Mediators of Inflammation

    Article Title: Role of Calprotectin as a Modulator of the IL27-Mediated Proinflammatory Effect on Endothelial Cells

    doi: 10.1155/2015/737310

    Figure Lengend Snippet: Hypothetical model of the calprotectin modulatory effects on IL27-mediated gene and protein expression based on our experimental results and literature data. GBP1, guanylate binding protein 1; NID1, nidogen-1; PECAM1, platelet endothelial cell adhesion molecule; TPM1, tropomyosin 1.

    Article Snippet: This scavenger function does not directly imply a prevention of receptor binding and subsequent activation of the signaling pathway, as it depends on the availability of free binding sites of IL27.

    Techniques: Expressing, Binding Assay

    Effects of IL27, calprotectin, and IL27/calprotectin cotreatment on gene expression. Relative mRNA levels of IL7, IL15, CXCL10, and CXCL11 of IL27 (30 ng/mL) ± calprotectin (1 μ g/mL)-stimulated HUVECs for 3, 6, 12, and 24 h are represented as mean ± SEM ( n = 6). Indicated p values are corresponding to significant differences between IL27 and IL27 + calprotectin: ∗ p

    Journal: Mediators of Inflammation

    Article Title: Role of Calprotectin as a Modulator of the IL27-Mediated Proinflammatory Effect on Endothelial Cells

    doi: 10.1155/2015/737310

    Figure Lengend Snippet: Effects of IL27, calprotectin, and IL27/calprotectin cotreatment on gene expression. Relative mRNA levels of IL7, IL15, CXCL10, and CXCL11 of IL27 (30 ng/mL) ± calprotectin (1 μ g/mL)-stimulated HUVECs for 3, 6, 12, and 24 h are represented as mean ± SEM ( n = 6). Indicated p values are corresponding to significant differences between IL27 and IL27 + calprotectin: ∗ p

    Article Snippet: This scavenger function does not directly imply a prevention of receptor binding and subsequent activation of the signaling pathway, as it depends on the availability of free binding sites of IL27.

    Techniques: Expressing

    Venn diagrams on IL27- and calprotectin-stimulated HUVECs including significant regulated genes with p value p

    Journal: Mediators of Inflammation

    Article Title: Role of Calprotectin as a Modulator of the IL27-Mediated Proinflammatory Effect on Endothelial Cells

    doi: 10.1155/2015/737310

    Figure Lengend Snippet: Venn diagrams on IL27- and calprotectin-stimulated HUVECs including significant regulated genes with p value p

    Article Snippet: This scavenger function does not directly imply a prevention of receptor binding and subsequent activation of the signaling pathway, as it depends on the availability of free binding sites of IL27.

    Techniques:

    Qualitative representation of the calprotectin modulatory effects on IL27-mediated protein and gene expression in HUVECs. Colour code: red: upregulation, bold red (arrow up): increased upregulation compared to IL27 stimulation only, dark red (arrow down): decreased upregulation compared to IL27 stimulation only, and green: downregulation. IL27 receptor: gp130/WSX-1 and calprotectin receptors: RAGE (advanced glucated end product receptor) and TLR4 (Toll-like receptor 4).

    Journal: Mediators of Inflammation

    Article Title: Role of Calprotectin as a Modulator of the IL27-Mediated Proinflammatory Effect on Endothelial Cells

    doi: 10.1155/2015/737310

    Figure Lengend Snippet: Qualitative representation of the calprotectin modulatory effects on IL27-mediated protein and gene expression in HUVECs. Colour code: red: upregulation, bold red (arrow up): increased upregulation compared to IL27 stimulation only, dark red (arrow down): decreased upregulation compared to IL27 stimulation only, and green: downregulation. IL27 receptor: gp130/WSX-1 and calprotectin receptors: RAGE (advanced glucated end product receptor) and TLR4 (Toll-like receptor 4).

    Article Snippet: This scavenger function does not directly imply a prevention of receptor binding and subsequent activation of the signaling pathway, as it depends on the availability of free binding sites of IL27.

    Techniques: Expressing